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The map label for this gene is hemA

Identifier: 197116805

GI number: 197116805

Start: 482440

End: 483744

Strand: Direct

Name: hemA

Synonym: Gbem_0407

Alternate gene names: 197116805

Gene position: 482440-483744 (Clockwise)

Preceding gene: 197116804

Following gene: 197116806

Centisome position: 10.45

GC content: 60.69

Gene sequence:

>1305_bases
ATGAATATTATTGTGGTTGGGCTGTCGCACAAAACTGCCGCAGTTGAAATAAGGGAAAAAGTAGCCTTCGCCCCGACCCA
GATGGAAAAACCGCTCCAGGCTGTCGTGGCGCTGCCCGACATCACCGAGGCGGTCATCGTCTCCACCTGCAACAGGGTCG
AGATCTACGCCACCACGCGCGACGTCGCTGGGGGGATGGCGAGGATCAAGCGTTTCCTGGCGGATTACCACAACGTCCCC
CTGGAGTTGCTGGAGAAGCACCTCTACAGCTACCACGGCGAGGACGCCACCCGTCACGTCTTCCGGGTCGCCTCCAGCCT
CGATTCCATGGTGGTCGGCGAGCCGCAGATACTCGGGCAGATCAAGACCTCCTACGGTTACGCCGCCGAGTACAAGAGCT
CCGGCATCATCCTGAACCGCTTCCTGCACAAGGCCTTCTCCGTTGCCAAGAGGGTCCGCACCGAGACCAAAATCGCCTCC
TCCGCCGTATCGGTCGCCTTTGCAGCCGTCGAGTTGGCCAAGAAAATCTTCGGGGAACTCTCCGACAAGACCGTCCTTCT
GATCGGCGCCGGCGAGATGTGCGAGCTTGCCGCCAAGCACTTCATCAACACCGGCGTGCGCGGCGTCATGGTCACCAACC
GCACCTACGAGCGCGCCGAAAAGCTGGCCGAGGAATTCGACGCGAAGCCGGTTCATTTCGAGGCGTTGATGGAGACCCTT
CCCAAGGCCGACATCATCCTCTCCTCCACCGGCGCGCCGCACTTCATCATCCACGAAAAGGATATGGAGGAAGTGCTGCG
CCGCCGCAAGCACAAGCCCATGTTCTTCATCGACATCGCCGTGCCGCGCGACATCGACCCCAAGGTCAACAACGTGGAGA
ACTGTTATCTCTACACCGTCGACGATCTGAACGGCGTCGTCGCCACCAACCTGGAACAGCGCAAGGTCGAGGCGGCCAAG
GCCGAGGCCATCGTCGAGCAGGAGATCGGGCAGTTCTTCAAGTGGCTCTCCTCGCTGGAGGTCACCCCGACCATCGTGGC
GCTCAGGACCCACTTCGACGAGATCCGCAAGGCCGAGCTCTCCAAGACCATCTCCGGGTGGAAGGACCTTCCCCCCGGCG
CCGAGAAGAAGCTCGATGCGCTCACCAACGCCATCATGAACAAGCTGTTGCACCAGCCCACCAGCGTGCTGAAGAGGATC
GACCAGGGAAACCGCAACGACCTCTATGTCGACGCGCTGAGGAACCTATTCGACCTCGAAGTCGGGGGGGAGTCGCAGGA
CAACATGATGGAACTGGAAGACTGA

Upstream 100 bases:

>100_bases
CGTGAATCTTCTCCTGTCGGGGCTGCACAGCTACAAGTAAGGGCAACAAGAGGCGGCAACCGCTTTTCGGTTGTGATTCT
CAGGAAGAGGCAGGTTTGTT

Downstream 100 bases:

>100_bases
GAAGCAGGGGCTAGGGGCTGAAAAACCGGGGCTAGGGGCTGGGGGCTGGAACTGCCTGAACCGGCGACGGGTCCGGTCAA
TATCACTTTATCCAAGGGAT

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNIIVVGLSHKTAAVEIREKVAFAPTQMEKPLQAVVALPDITEAVIVSTCNRVEIYATTRDVAGGMARIKRFLADYHNVP
LELLEKHLYSYHGEDATRHVFRVASSLDSMVVGEPQILGQIKTSYGYAAEYKSSGIILNRFLHKAFSVAKRVRTETKIAS
SAVSVAFAAVELAKKIFGELSDKTVLLIGAGEMCELAAKHFINTGVRGVMVTNRTYERAEKLAEEFDAKPVHFEALMETL
PKADIILSSTGAPHFIIHEKDMEEVLRRRKHKPMFFIDIAVPRDIDPKVNNVENCYLYTVDDLNGVVATNLEQRKVEAAK
AEAIVEQEIGQFFKWLSSLEVTPTIVALRTHFDEIRKAELSKTISGWKDLPPGAEKKLDALTNAIMNKLLHQPTSVLKRI
DQGNRNDLYVDALRNLFDLEVGGESQDNMMELED

Sequences:

>Translated_434_residues
MNIIVVGLSHKTAAVEIREKVAFAPTQMEKPLQAVVALPDITEAVIVSTCNRVEIYATTRDVAGGMARIKRFLADYHNVP
LELLEKHLYSYHGEDATRHVFRVASSLDSMVVGEPQILGQIKTSYGYAAEYKSSGIILNRFLHKAFSVAKRVRTETKIAS
SAVSVAFAAVELAKKIFGELSDKTVLLIGAGEMCELAAKHFINTGVRGVMVTNRTYERAEKLAEEFDAKPVHFEALMETL
PKADIILSSTGAPHFIIHEKDMEEVLRRRKHKPMFFIDIAVPRDIDPKVNNVENCYLYTVDDLNGVVATNLEQRKVEAAK
AEAIVEQEIGQFFKWLSSLEVTPTIVALRTHFDEIRKAELSKTISGWKDLPPGAEKKLDALTNAIMNKLLHQPTSVLKRI
DQGNRNDLYVDALRNLFDLEVGGESQDNMMELED
>Mature_434_residues
MNIIVVGLSHKTAAVEIREKVAFAPTQMEKPLQAVVALPDITEAVIVSTCNRVEIYATTRDVAGGMARIKRFLADYHNVP
LELLEKHLYSYHGEDATRHVFRVASSLDSMVVGEPQILGQIKTSYGYAAEYKSSGIILNRFLHKAFSVAKRVRTETKIAS
SAVSVAFAAVELAKKIFGELSDKTVLLIGAGEMCELAAKHFINTGVRGVMVTNRTYERAEKLAEEFDAKPVHFEALMETL
PKADIILSSTGAPHFIIHEKDMEEVLRRRKHKPMFFIDIAVPRDIDPKVNNVENCYLYTVDDLNGVVATNLEQRKVEAAK
AEAIVEQEIGQFFKWLSSLEVTPTIVALRTHFDEIRKAELSKTISGWKDLPPGAEKKLDALTNAIMNKLLHQPTSVLKRI
DQGNRNDLYVDALRNLFDLEVGGESQDNMMELED

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=422, Percent_Identity=41.4691943127962, Blast_Score=318, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_GEOBB (B5EBG7)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137232.1
- ProteinModelPortal:   B5EBG7
- SMR:   B5EBG7
- GeneID:   6783233
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0407
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- ProtClustDB:   PRK00045
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR003462
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR13812
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 48493; Mature: 48493

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIVVGLSHKTAAVEIREKVAFAPTQMEKPLQAVVALPDITEAVIVSTCNRVEIYATTR
CCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECH
DVAGGMARIKRFLADYHNVPLELLEKHLYSYHGEDATRHVFRVASSLDSMVVGEPQILGQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
IKTSYGYAAEYKSSGIILNRFLHKAFSVAKRVRTETKIASSAVSVAFAAVELAKKIFGEL
HHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SDKTVLLIGAGEMCELAAKHFINTGVRGVMVTNRTYERAEKLAEEFDAKPVHFEALMETL
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHC
PKADIILSSTGAPHFIIHEKDMEEVLRRRKHKPMFFIDIAVPRDIDPKVNNVENCYLYTV
CCCCEEEECCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEE
DDLNGVVATNLEQRKVEAAKAEAIVEQEIGQFFKWLSSLEVTPTIVALRTHFDEIRKAEL
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SKTISGWKDLPPGAEKKLDALTNAIMNKLLHQPTSVLKRIDQGNRNDLYVDALRNLFDLE
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHCCC
VGGESQDNMMELED
CCCCCCCCCEECCC
>Mature Secondary Structure
MNIIVVGLSHKTAAVEIREKVAFAPTQMEKPLQAVVALPDITEAVIVSTCNRVEIYATTR
CCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECH
DVAGGMARIKRFLADYHNVPLELLEKHLYSYHGEDATRHVFRVASSLDSMVVGEPQILGQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
IKTSYGYAAEYKSSGIILNRFLHKAFSVAKRVRTETKIASSAVSVAFAAVELAKKIFGEL
HHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SDKTVLLIGAGEMCELAAKHFINTGVRGVMVTNRTYERAEKLAEEFDAKPVHFEALMETL
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHC
PKADIILSSTGAPHFIIHEKDMEEVLRRRKHKPMFFIDIAVPRDIDPKVNNVENCYLYTV
CCCCEEEECCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEE
DDLNGVVATNLEQRKVEAAKAEAIVEQEIGQFFKWLSSLEVTPTIVALRTHFDEIRKAEL
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SKTISGWKDLPPGAEKKLDALTNAIMNKLLHQPTSVLKRIDQGNRNDLYVDALRNLFDLE
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHCCC
VGGESQDNMMELED
CCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA