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The map label for this gene is pleD [H]

Identifier: 197116776

GI number: 197116776

Start: 453811

End: 454425

Strand: Reverse

Name: pleD [H]

Synonym: Gbem_0378

Alternate gene names: 197116776

Gene position: 454425-453811 (Counterclockwise)

Preceding gene: 197116777

Following gene: 197116771

Centisome position: 9.85

GC content: 57.72

Gene sequence:

>615_bases
GTGGTCAAGAAAAAGCTGCTGGTTGTCGACGACACGCCGGAAAACCTGATCATTCTCTACAAGATCTTGCGCAAGGAGTA
CGAGGTCCTTGGAGCCAACAGCGGCAAGGAAGCGCTGCTGGCCCTGGCCGAGAATCGTCCCGACCTGATCCTGCTCGACG
TCATGATGCCCGAAATGGACGGACTGGAGGTCTGCCGCATCCTGAAGGACGATTCCAGATACCGGGACATCCCGGTCATC
TTCGTAACCGCGCTGAGCGACGAAGTGGACGAGTCACGGGGATTCAAAGCCGGCGCGGTAGACTTCCTCTCCAAGCCGGT
CAATCCCGTCATCCTGAGCCATCGTGTCGCAGTGCATCTAGAACTGCAGTCCCAAAAAGAAGCACTGACCCGGAAGAACC
AGGAACTGCAGGAAGCCCTCTCCAGGGTCAAGGAGCTATCCGGGCTGCTCCCCATCTGCATGACCTGCAAAAAGATCCGT
GACGACCAGGGGTATTGGAACCAGTTGGAGAGCTACATCTCGCTCCACTCGGAGGCCCTTTTCAGCCACGGCTACTGTCC
GGAATGCGCCGCTGCCGCGATGAAAAAGATCCTACCGGTGCCGAACTTGCGATAG

Upstream 100 bases:

>100_bases
TTGCCAAAGCGCTCTTAATACAGTCGGACAAATCCTCCTGAATTACGACATCCACTCCTGATGCAGTTTCTGTGCATTGC
CACAGAGAAATGAGGATACC

Downstream 100 bases:

>100_bases
CAGCACCCTCCCCCCCGCACCGCCCGGCGCAGGCCTACCTCGCCAGTACGAAATCGGCTATTGCATGGGCGATGCGGTCG
GTGGGGCAGCGGTCGTTGTT

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MVKKKLLVVDDTPENLIILYKILRKEYEVLGANSGKEALLALAENRPDLILLDVMMPEMDGLEVCRILKDDSRYRDIPVI
FVTALSDEVDESRGFKAGAVDFLSKPVNPVILSHRVAVHLELQSQKEALTRKNQELQEALSRVKELSGLLPICMTCKKIR
DDQGYWNQLESYISLHSEALFSHGYCPECAAAAMKKILPVPNLR

Sequences:

>Translated_204_residues
MVKKKLLVVDDTPENLIILYKILRKEYEVLGANSGKEALLALAENRPDLILLDVMMPEMDGLEVCRILKDDSRYRDIPVI
FVTALSDEVDESRGFKAGAVDFLSKPVNPVILSHRVAVHLELQSQKEALTRKNQELQEALSRVKELSGLLPICMTCKKIR
DDQGYWNQLESYISLHSEALFSHGYCPECAAAAMKKILPVPNLR
>Mature_204_residues
MVKKKLLVVDDTPENLIILYKILRKEYEVLGANSGKEALLALAENRPDLILLDVMMPEMDGLEVCRILKDDSRYRDIPVI
FVTALSDEVDESRGFKAGAVDFLSKPVNPVILSHRVAVHLELQSQKEALTRKNQELQEALSRVKELSGLLPICMTCKKIR
DDQGYWNQLESYISLHSEALFSHGYCPECAAAAMKKILPVPNLR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=116, Percent_Identity=35.3448275862069, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1788394, Length=132, Percent_Identity=34.0909090909091, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788550, Length=160, Percent_Identity=29.375, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=34.1463414634146, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=40.3846153846154, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 23020; Mature: 23020

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKKLLVVDDTPENLIILYKILRKEYEVLGANSGKEALLALAENRPDLILLDVMMPEMD
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCC
GLEVCRILKDDSRYRDIPVIFVTALSDEVDESRGFKAGAVDFLSKPVNPVILSHRVAVHL
HHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHCCCCHHEECCEEEEEE
ELQSQKEALTRKNQELQEALSRVKELSGLLPICMTCKKIRDDQGYWNQLESYISLHSEAL
EEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FSHGYCPECAAAAMKKILPVPNLR
HHCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVKKKLLVVDDTPENLIILYKILRKEYEVLGANSGKEALLALAENRPDLILLDVMMPEMD
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCC
GLEVCRILKDDSRYRDIPVIFVTALSDEVDESRGFKAGAVDFLSKPVNPVILSHRVAVHL
HHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHCCCCHHEECCEEEEEE
ELQSQKEALTRKNQELQEALSRVKELSGLLPICMTCKKIRDDQGYWNQLESYISLHSEAL
EEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FSHGYCPECAAAAMKKILPVPNLR
HHCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA