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The map label for this gene is rne [H]

Identifier: 195953000

GI number: 195953000

Start: 558285

End: 559439

Strand: Direct

Name: rne [H]

Synonym: HY04AAS1_0625

Alternate gene names: 195953000

Gene position: 558285-559439 (Clockwise)

Preceding gene: 195952999

Following gene: 195953001

Centisome position: 35.8

GC content: 29.96

Gene sequence:

>1155_bases
ATGAAATTGGTAGTATATAAAAAGAATGATGTAATATTTTCAATGCTTTTTAAAAGTAACGAACTTTTAGATATTAAAGT
AGACAATTTAAACGATCCTGAATATAGCTCATCTATTTTTTTAGGTAAGATAAAATCTATCGCAAACGGAGTAGAAGGAT
ATTTTGTTGATATAGGTTTAAAAAAAGATGGGTATCTGCCTTTCAAAGAAGCTGATAAAGAATATAAAATAGGAGATTAT
GTTGTTGTTCAAGTAAAAAAAGAACCATCCAAATTTAAAGGAGCTAAATTAACCACCAAACTCTCTTTTAAAGGAAAGTA
CATAATATATTATGAGGCTGATAAATCTGTAAAAACCTCACATCTTTTTGATGAGAATTTTAAAAGCGATTACATTGATA
AAGTTTATAGGCTTTTAAGAGAAAAAGAATCTGTAATTATTAGGACTCAAGCTAAAAATGTAGACATATCGAGAATAGGA
CAAGAGTTGGAATTTTATAGGAAAAAAGCAAAACTCATAAGAGACATATCTCACAAAGCTATTATGCAATTAGAACCTAA
AGAGCCATGCTATGTATCAATGATAAAAGATTATGTTGGTGGTATAGAGAAAATTGTAGTATCTGATATAAAAGTTTACA
ACAATATAGTGGGTTTCATATTGTCAAACGATTTAAATATAAACGTTGAAATGATTATTGATAAAGAGAGTTTAGAAAAC
TTATACGAAGTAGAAAAGCACATCAACAGGCTTTTAAGCAAGTATGTATGGTTAGAAAGCGGTGGTTTTTTAGTAATAGA
AGAGACAGAGGCTATGACTACAATAGATGTAAATAGCGGTAAAAATACCTGTGATTGTATAGAATCAAGTTCTTACTCCA
CAAACCTGGAAGCAGCCAAAGAAATACCAAGACAAATAAAGCTTAGAAATATAGGTGGTATCATAATGATAGATTTTATT
TTCATGAAAGATCAAGTTCATAGAAAAGAGGTGCTTGATGTATTGAAAAATGGCTTTTCAAAAGACAACGTCCAAACCAC
AGTCTATGGATATACGGCTCTTGGGCTTATGGAGATAGCAAGAAAAAAAACTGGAAGAAGCTTATTCCAAACCATAAAAG
CATCTAAACAAGAAAATATGCCAAGTGTTGTATGA

Upstream 100 bases:

>100_bases
AACAAGCTTATGGATATGTTAGGGCAGTTTTTAGATGGCTTAGAAGATGAATCTTTTGATAGAGCGGCTAAAATATTAAA
AATGTTAGATAATATAAAAG

Downstream 100 bases:

>100_bases
AAGAGTTATAATAATTACTTATTACATTTTGGAGGTTTTTAGGAAATGGCAAAACTCAAAGGACTAAAATGTAGAGAATG
CAAGCGTGAATATCCTATAG

Product: RNA binding S1 domain-containing protein

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKLVVYKKNDVIFSMLFKSNELLDIKVDNLNDPEYSSSIFLGKIKSIANGVEGYFVDIGLKKDGYLPFKEADKEYKIGDY
VVVQVKKEPSKFKGAKLTTKLSFKGKYIIYYEADKSVKTSHLFDENFKSDYIDKVYRLLREKESVIIRTQAKNVDISRIG
QELEFYRKKAKLIRDISHKAIMQLEPKEPCYVSMIKDYVGGIEKIVVSDIKVYNNIVGFILSNDLNINVEMIIDKESLEN
LYEVEKHINRLLSKYVWLESGGFLVIEETEAMTTIDVNSGKNTCDCIESSSYSTNLEAAKEIPRQIKLRNIGGIIMIDFI
FMKDQVHRKEVLDVLKNGFSKDNVQTTVYGYTALGLMEIARKKTGRSLFQTIKASKQENMPSVV

Sequences:

>Translated_384_residues
MKLVVYKKNDVIFSMLFKSNELLDIKVDNLNDPEYSSSIFLGKIKSIANGVEGYFVDIGLKKDGYLPFKEADKEYKIGDY
VVVQVKKEPSKFKGAKLTTKLSFKGKYIIYYEADKSVKTSHLFDENFKSDYIDKVYRLLREKESVIIRTQAKNVDISRIG
QELEFYRKKAKLIRDISHKAIMQLEPKEPCYVSMIKDYVGGIEKIVVSDIKVYNNIVGFILSNDLNINVEMIIDKESLEN
LYEVEKHINRLLSKYVWLESGGFLVIEETEAMTTIDVNSGKNTCDCIESSSYSTNLEAAKEIPRQIKLRNIGGIIMIDFI
FMKDQVHRKEVLDVLKNGFSKDNVQTTVYGYTALGLMEIARKKTGRSLFQTIKASKQENMPSVV
>Mature_384_residues
MKLVVYKKNDVIFSMLFKSNELLDIKVDNLNDPEYSSSIFLGKIKSIANGVEGYFVDIGLKKDGYLPFKEADKEYKIGDY
VVVQVKKEPSKFKGAKLTTKLSFKGKYIIYYEADKSVKTSHLFDENFKSDYIDKVYRLLREKESVIIRTQAKNVDISRIG
QELEFYRKKAKLIRDISHKAIMQLEPKEPCYVSMIKDYVGGIEKIVVSDIKVYNNIVGFILSNDLNINVEMIIDKESLEN
LYEVEKHINRLLSKYVWLESGGFLVIEETEAMTTIDVNSGKNTCDCIESSSYSTNLEAAKEIPRQIKLRNIGGIIMIDFI
FMKDQVHRKEVLDVLKNGFSKDNVQTTVYGYTALGLMEIARKKTGRSLFQTIKASKQENMPSVV

Specific function: This protein matures 5S rRNA from its precursors from all the rRNA genes. It is the major endoribonuclease participating in mRNA turnover [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=363, Percent_Identity=30.8539944903581, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787325, Length=382, Percent_Identity=28.2722513089005, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 44041; Mature: 44041

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVVYKKNDVIFSMLFKSNELLDIKVDNLNDPEYSSSIFLGKIKSIANGVEGYFVDIGL
CEEEEEECCCEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEE
KKDGYLPFKEADKEYKIGDYVVVQVKKEPSKFKGAKLTTKLSFKGKYIIYYEADKSVKTS
CCCCCCCHHHCCCCEECCCEEEEEEECCCCCCCCCEEEEEEEECCCEEEEEECCCCCCHH
HLFDENFKSDYIDKVYRLLREKESVIIRTQAKNVDISRIGQELEFYRKKAKLIRDISHKA
HHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHH
IMQLEPKEPCYVSMIKDYVGGIEKIVVSDIKVYNNIVGFILSNDLNINVEMIIDKESLEN
HEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEECHHHHHH
LYEVEKHINRLLSKYVWLESGGFLVIEETEAMTTIDVNSGKNTCDCIESSSYSTNLEAAK
HHHHHHHHHHHHHHHEEEECCCEEEEECCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHH
EIPRQIKLRNIGGIIMIDFIFMKDQVHRKEVLDVLKNGFSKDNVQTTVYGYTALGLMEIA
HCHHHEEHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHH
RKKTGRSLFQTIKASKQENMPSVV
HHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLVVYKKNDVIFSMLFKSNELLDIKVDNLNDPEYSSSIFLGKIKSIANGVEGYFVDIGL
CEEEEEECCCEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEE
KKDGYLPFKEADKEYKIGDYVVVQVKKEPSKFKGAKLTTKLSFKGKYIIYYEADKSVKTS
CCCCCCCHHHCCCCEECCCEEEEEEECCCCCCCCCEEEEEEEECCCEEEEEECCCCCCHH
HLFDENFKSDYIDKVYRLLREKESVIIRTQAKNVDISRIGQELEFYRKKAKLIRDISHKA
HHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHH
IMQLEPKEPCYVSMIKDYVGGIEKIVVSDIKVYNNIVGFILSNDLNINVEMIIDKESLEN
HEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEECHHHHHH
LYEVEKHINRLLSKYVWLESGGFLVIEETEAMTTIDVNSGKNTCDCIESSSYSTNLEAAK
HHHHHHHHHHHHHHHEEEECCCEEEEECCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHH
EIPRQIKLRNIGGIIMIDFIFMKDQVHRKEVLDVLKNGFSKDNVQTTVYGYTALGLMEIA
HCHHHEEHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHH
RKKTGRSLFQTIKASKQENMPSVV
HHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]