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The map label for this gene is otsA [H]

Identifier: 194736914

GI number: 194736914

Start: 2001187

End: 2002608

Strand: Reverse

Name: otsA [H]

Synonym: SeSA_A2083

Alternate gene names: 194736914

Gene position: 2002608-2001187 (Counterclockwise)

Preceding gene: 194736574

Following gene: 194737939

Centisome position: 42.53

GC content: 52.39

Gene sequence:

>1422_bases
ATGAGTCGTTTAGTCGTAGTATCTAATCGAATTGCCCCCCCGGATAATAAAGGCGGCGCCGGCGGCCTCGCCGTTGGCGT
GCTTGGCGCGCTAAAAGCGGCTGGCGGGTTGTGGTTCGGCTGGAGTGGCGAGACAGGTAACGAGGATGAGCCATTAAAAA
AGGTGACAAAAGGTAATATTACCTGGGCATCGTTTAACCTGAGTGAACAAGATTACGAAGATTATTACTGTCAATTTTCC
AATGCGGTTCTCTGGCCTGCGTTCCATTATCGTCTGGACCTGGTACAGTTTCAGCGTCCTGCATGGGAAGGCTATATGCG
GGTGAATGCGTTATTAGCGGATAAGTTATTGCCCCTCATTAAAGAGAACGACATCATTTGGGTGCATGACTACCACCTGT
TACCGTTCGCCAGCGAGCTGCGTAAACGCGGCGTGAACAACCGAATTGGTTTTTTCCTGCATATTCCATTCCCGACCCCG
GAGATTTTTAACGCTTTACCGCCGCATGATGAACTGCTGGAGCAGTTGTGTGACTTTGATCTGCTCGGGTTCCAGACCGA
AAATGATCGCCTGGCTTTTCTGGATAGCCTTTCGAGTCAAACGCGAGTCACGACTCGCAGCGGCAAGCAGCATATGGCGT
GGGGTAAAGACTTCCAGACAGAAGTGTATCCTATCGGTATTGAGCCCGATGAGATTGCTCTGCAGGCAGCCGGGCCGTTG
CCGCCTAAACTGGCGCAGCTCAAGGCGGAACTGAAAAATGTGAAGAATATTTTTTCCGTTGAGCGGCTGGATTATTCGAA
AGGGCTGCCGGAACGCTTTCTGGCGTATGAAGCGCTACTGGAAAACTACCCGCAGCATCGGGGAAAAATTCGTTATACCC
AAATTGCGCCTACGTCACGCGGCGAAGTACAGGCATATCAGGATATTCGCCACCAGCTTGAGACGGAAGCAGGCCGGATT
AATGGGAAATATGGACAATTGGGCTGGACGCCGCTCTATTATCTGAATCAGCATTTTGACCGTAAACTGTTAATGAAGAT
ATTCCGTTATTCAGACGTCGGGCTCGTCACCCCGTTGCGTGACGGGATGAACCTGGTGGCGAAAGAGTTTGTCGCCGCGC
AGGACCCCGCTAATCCTGGCGTACTGGTACTGTCACAGTTTGCCGGCGCGGCGAATGAACTGACGTCGGCGTTAATCGTC
AATCCTTACGATCGGGATGACGTGGCGGCGGCGCTCAATCGTGCGTTAACGATGCCCCTTGCCGAGCGTATTTCGCGCCA
TGCGGAAATGCTGGACGTGATCGTTAAAAATGACATTAACCGCTGGCAGGAGCGTTTTATTCATGACCTAAAGGAGGTCA
CGCCGCGTAGCCCTGAGCGTCAGCAGCAGAACAACGTGGCGACGTTCCCTAAGCTGGCCTGA

Upstream 100 bases:

>100_bases
GCATGGCGGCTGGAAAGCGTACCCGATGTCTGGCGCTGGCTGGAGCAAATCAATTATCCACAACAAGAACAACAAGTAAT
GAATAACAGGAGAGATGGCT

Downstream 100 bases:

>100_bases
AATATTGCGAGCGGCGACTAGTCGCCCGCTAACGGCACCAGCAACACATCTACCTGGCTGGCGCTGACGACACTTTTTGC
CGAGCAGGACGCGCGCGAAA

Product: trehalose-6-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSRLVVVSNRIAPPDNKGGAGGLAVGVLGALKAAGGLWFGWSGETGNEDEPLKKVTKGNITWASFNLSEQDYEDYYCQFS
NAVLWPAFHYRLDLVQFQRPAWEGYMRVNALLADKLLPLIKENDIIWVHDYHLLPFASELRKRGVNNRIGFFLHIPFPTP
EIFNALPPHDELLEQLCDFDLLGFQTENDRLAFLDSLSSQTRVTTRSGKQHMAWGKDFQTEVYPIGIEPDEIALQAAGPL
PPKLAQLKAELKNVKNIFSVERLDYSKGLPERFLAYEALLENYPQHRGKIRYTQIAPTSRGEVQAYQDIRHQLETEAGRI
NGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEFVAAQDPANPGVLVLSQFAGAANELTSALIV
NPYDRDDVAAALNRALTMPLAERISRHAEMLDVIVKNDINRWQERFIHDLKEVTPRSPERQQQNNVATFPKLA

Sequences:

>Translated_473_residues
MSRLVVVSNRIAPPDNKGGAGGLAVGVLGALKAAGGLWFGWSGETGNEDEPLKKVTKGNITWASFNLSEQDYEDYYCQFS
NAVLWPAFHYRLDLVQFQRPAWEGYMRVNALLADKLLPLIKENDIIWVHDYHLLPFASELRKRGVNNRIGFFLHIPFPTP
EIFNALPPHDELLEQLCDFDLLGFQTENDRLAFLDSLSSQTRVTTRSGKQHMAWGKDFQTEVYPIGIEPDEIALQAAGPL
PPKLAQLKAELKNVKNIFSVERLDYSKGLPERFLAYEALLENYPQHRGKIRYTQIAPTSRGEVQAYQDIRHQLETEAGRI
NGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEFVAAQDPANPGVLVLSQFAGAANELTSALIV
NPYDRDDVAAALNRALTMPLAERISRHAEMLDVIVKNDINRWQERFIHDLKEVTPRSPERQQQNNVATFPKLA
>Mature_472_residues
SRLVVVSNRIAPPDNKGGAGGLAVGVLGALKAAGGLWFGWSGETGNEDEPLKKVTKGNITWASFNLSEQDYEDYYCQFSN
AVLWPAFHYRLDLVQFQRPAWEGYMRVNALLADKLLPLIKENDIIWVHDYHLLPFASELRKRGVNNRIGFFLHIPFPTPE
IFNALPPHDELLEQLCDFDLLGFQTENDRLAFLDSLSSQTRVTTRSGKQHMAWGKDFQTEVYPIGIEPDEIALQAAGPLP
PKLAQLKAELKNVKNIFSVERLDYSKGLPERFLAYEALLENYPQHRGKIRYTQIAPTSRGEVQAYQDIRHQLETEAGRIN
GKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEFVAAQDPANPGVLVLSQFAGAANELTSALIVN
PYDRDDVAAALNRALTMPLAERISRHAEMLDVIVKNDINRWQERFIHDLKEVTPRSPERQQQNNVATFPKLA

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=474, Percent_Identity=88.6075949367088, Blast_Score=848, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71987012, Length=435, Percent_Identity=30.3448275862069, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI115533558, Length=425, Percent_Identity=29.1764705882353, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI115533560, Length=425, Percent_Identity=29.1764705882353, Blast_Score=162, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6319602, Length=467, Percent_Identity=34.9036402569593, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6320279, Length=396, Percent_Identity=28.5353535353535, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323537, Length=392, Percent_Identity=27.5510204081633, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6323917, Length=446, Percent_Identity=27.5784753363229, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI19920676, Length=486, Percent_Identity=34.5679012345679, Blast_Score=278, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 53600; Mature: 53468

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVVVSNRIAPPDNKGGAGGLAVGVLGALKAAGGLWFGWSGETGNEDEPLKKVTKGNI
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCCE
TWASFNLSEQDYEDYYCQFSNAVLWPAFHYRLDLVQFQRPAWEGYMRVNALLADKLLPLI
EEEEECCCCHHHHHHHHHHCCEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KENDIIWVHDYHLLPFASELRKRGVNNRIGFFLHIPFPTPEIFNALPPHDELLEQLCDFD
CCCCEEEEECCCCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCHHHHHHHHCCCC
LLGFQTENDRLAFLDSLSSQTRVTTRSGKQHMAWGKDFQTEVYPIGIEPDEIALQAAGPL
CCCEECCCCCCHHHHHCCCCCEEECCCCCHHCCCCCCCCCCEEEECCCCCCEEEEECCCC
PPKLAQLKAELKNVKNIFSVERLDYSKGLPERFLAYEALLENYPQHRGKIRYTQIAPTSR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCCEEEEEECCCCC
GEVQAYQDIRHQLETEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPLR
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCEECCHH
DGMNLVAKEFVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDDVAAALNRALTMPL
HHHHHHHHHHHHHCCCCCCCEEEHHHHCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHH
AERISRHAEMLDVIVKNDINRWQERFIHDLKEVTPRSPERQQQNNVATFPKLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
SRLVVVSNRIAPPDNKGGAGGLAVGVLGALKAAGGLWFGWSGETGNEDEPLKKVTKGNI
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCCE
TWASFNLSEQDYEDYYCQFSNAVLWPAFHYRLDLVQFQRPAWEGYMRVNALLADKLLPLI
EEEEECCCCHHHHHHHHHHCCEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KENDIIWVHDYHLLPFASELRKRGVNNRIGFFLHIPFPTPEIFNALPPHDELLEQLCDFD
CCCCEEEEECCCCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCHHHHHHHHCCCC
LLGFQTENDRLAFLDSLSSQTRVTTRSGKQHMAWGKDFQTEVYPIGIEPDEIALQAAGPL
CCCEECCCCCCHHHHHCCCCCEEECCCCCHHCCCCCCCCCCEEEECCCCCCEEEEECCCC
PPKLAQLKAELKNVKNIFSVERLDYSKGLPERFLAYEALLENYPQHRGKIRYTQIAPTSR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCCEEEEEECCCCC
GEVQAYQDIRHQLETEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPLR
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCEECCHH
DGMNLVAKEFVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDDVAAALNRALTMPL
HHHHHHHHHHHHHCCCCCCCEEEHHHHCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHH
AERISRHAEMLDVIVKNDINRWQERFIHDLKEVTPRSPERQQQNNVATFPKLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA