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The map label for this gene is btuE [H]

Identifier: 194736242

GI number: 194736242

Start: 1384611

End: 1385162

Strand: Direct

Name: btuE [H]

Synonym: SeSA_A1436

Alternate gene names: 194736242

Gene position: 1384611-1385162 (Clockwise)

Preceding gene: 194737002

Following gene: 194736020

Centisome position: 29.4

GC content: 49.28

Gene sequence:

>552_bases
ATGCAAAACTCGCTTCTTAATACTCACGTTACGACGATTGATGGAGAGGTCACTACACTTGAAAAATATGCCGGAAAAGT
GCTGCTCATTGTAAACGTCGCCTCCAGATGTGGCCTGACGCCGCAATATGAACAGCTGGAAAATATCCAGAAGGCGTGGG
CAGATCAGGGGTTTGTCGTGTTGGGTTTTCCCTGCAACCAGTTTATGGGGCAGGAGCCAGGCAGCGAAGAAGAGATTAAA
ACCTATTGCGCCAGCACCTGGCACGTCACGTTCCCGATGTTCAGTAAAATTGATGTGAATGGCGACGCGCGCCATCCGCT
GTATCAAAAACTGATAGCCGCTGCGCCAACGGCAGTCGCGCCGGAGAAGAGCGGCTTTTACGAACGGATGGTAAGCAAAG
GGCGTACGCCACTCTATCCGGATGATATTCTGTGGAATTTTGAAAAATTTTTGGTAGGCCGGGATGGTCAGGTAGTACAG
CGTTTTTCGCCGGATATGACGCCTGAAGATCCTATTGTGATGGAAAGCATAAAAATCGCGTTGGCAAAATAA

Upstream 100 bases:

>100_bases
GCGCGTTAGCGCATCGGTAAACAACTGCTTTCATCATTCGCAGTCTATGATTAAAGGCTAACGATGAAACCCGCTATTCT
ACCGACATCAGGGAGACACC

Downstream 100 bases:

>100_bases
TGTCTCAATTGATGCAATTAAAGGATGTCGCCGAGAGCACCCGCCTGGGGCCGCTTTCCGGCGAAGTAAGCGCAGGAGAG
ATCCTTCATCTTGTCGGGCC

Product: putative glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MQNSLLNTHVTTIDGEVTTLEKYAGKVLLIVNVASRCGLTPQYEQLENIQKAWADQGFVVLGFPCNQFMGQEPGSEEEIK
TYCASTWHVTFPMFSKIDVNGDARHPLYQKLIAAAPTAVAPEKSGFYERMVSKGRTPLYPDDILWNFEKFLVGRDGQVVQ
RFSPDMTPEDPIVMESIKIALAK

Sequences:

>Translated_183_residues
MQNSLLNTHVTTIDGEVTTLEKYAGKVLLIVNVASRCGLTPQYEQLENIQKAWADQGFVVLGFPCNQFMGQEPGSEEEIK
TYCASTWHVTFPMFSKIDVNGDARHPLYQKLIAAAPTAVAPEKSGFYERMVSKGRTPLYPDDILWNFEKFLVGRDGQVVQ
RFSPDMTPEDPIVMESIKIALAK
>Mature_183_residues
MQNSLLNTHVTTIDGEVTTLEKYAGKVLLIVNVASRCGLTPQYEQLENIQKAWADQGFVVLGFPCNQFMGQEPGSEEEIK
TYCASTWHVTFPMFSKIDVNGDARHPLYQKLIAAAPTAVAPEKSGFYERMVSKGRTPLYPDDILWNFEKFLVGRDGQVVQ
RFSPDMTPEDPIVMESIKIALAK

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI75709200, Length=164, Percent_Identity=42.0731707317073, Blast_Score=118, Evalue=2e-27,
Organism=Homo sapiens, GI90903240, Length=173, Percent_Identity=39.8843930635838, Blast_Score=117, Evalue=4e-27,
Organism=Homo sapiens, GI15618997, Length=160, Percent_Identity=40, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI6006001, Length=162, Percent_Identity=41.9753086419753, Blast_Score=103, Evalue=8e-23,
Organism=Homo sapiens, GI4557629, Length=157, Percent_Identity=40.1273885350318, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI90903238, Length=139, Percent_Identity=41.726618705036, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI41406084, Length=155, Percent_Identity=40, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI192455698, Length=171, Percent_Identity=35.672514619883, Blast_Score=97, Evalue=9e-21,
Organism=Homo sapiens, GI33186887, Length=154, Percent_Identity=38.961038961039, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI32967607, Length=194, Percent_Identity=31.4432989690722, Blast_Score=84, Evalue=6e-17,
Organism=Escherichia coli, GI1788003, Length=183, Percent_Identity=85.2459016393443, Blast_Score=336, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI17506887, Length=181, Percent_Identity=41.4364640883978, Blast_Score=132, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17535473, Length=162, Percent_Identity=45.679012345679, Blast_Score=131, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17550320, Length=187, Percent_Identity=37.9679144385027, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI193209493, Length=187, Percent_Identity=35.8288770053476, Blast_Score=119, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71993584, Length=160, Percent_Identity=37.5, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI193210707, Length=160, Percent_Identity=36.25, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71993573, Length=95, Percent_Identity=36.8421052631579, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71999642, Length=76, Percent_Identity=42.1052631578947, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319721, Length=168, Percent_Identity=45.2380952380952, Blast_Score=143, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322228, Length=154, Percent_Identity=45.4545454545455, Blast_Score=135, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322826, Length=153, Percent_Identity=39.8692810457516, Blast_Score=109, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24656772, Length=163, Percent_Identity=36.1963190184049, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24656767, Length=169, Percent_Identity=35.5029585798817, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24656777, Length=163, Percent_Identity=36.1963190184049, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI85726443, Length=172, Percent_Identity=35.4651162790698, Blast_Score=97, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 20504; Mature: 20504

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1 ; PS00763 GLUTATHIONE_PEROXID_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSLLNTHVTTIDGEVTTLEKYAGKVLLIVNVASRCGLTPQYEQLENIQKAWADQGFVV
CCCCCCCCEEEEECCCHHHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHCCCCEEE
LGFPCNQFMGQEPGSEEEIKTYCASTWHVTFPMFSKIDVNGDARHPLYQKLIAAAPTAVA
ECCCHHHHCCCCCCCHHHHHHHHHCCEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCC
PEKSGFYERMVSKGRTPLYPDDILWNFEKFLVGRDGQVVQRFSPDMTPEDPIVMESIKIA
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHE
LAK
ECC
>Mature Secondary Structure
MQNSLLNTHVTTIDGEVTTLEKYAGKVLLIVNVASRCGLTPQYEQLENIQKAWADQGFVV
CCCCCCCCEEEEECCCHHHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHCCCCEEE
LGFPCNQFMGQEPGSEEEIKTYCASTWHVTFPMFSKIDVNGDARHPLYQKLIAAAPTAVA
ECCCHHHHCCCCCCCHHHHHHHHHCCEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCC
PEKSGFYERMVSKGRTPLYPDDILWNFEKFLVGRDGQVVQRFSPDMTPEDPIVMESIKIA
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHE
LAK
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]