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The map label for this gene is dagK [H]

Identifier: 194334651

GI number: 194334651

Start: 2024284

End: 2025189

Strand: Reverse

Name: dagK [H]

Synonym: Paes_1852

Alternate gene names: 194334651

Gene position: 2025189-2024284 (Counterclockwise)

Preceding gene: 194334652

Following gene: 194334650

Centisome position: 80.59

GC content: 50.66

Gene sequence:

>906_bases
ATGAAGTATACCTTTATTGTTAATCCCGTTGCAGGAAGCAGGCGCCTTTTCGCTCCTGCCACCGACATGCTGAAGACTCT
TTGCCGCACGAGGAGTGCGGACATCGTCGAGACCCGTCATGCCGGTCATGCCGGTGAACTTGCCGAAGAGTCATTACGCA
ACGGCGACGTTGTCATAGCCTGCGGGGGGGACGGTACTGCGCATGAGGTTGCAAATATTCTTGCATTTTCTGAACGGATT
ATGGGCGTTTTCCCTGCAGGATCAGCCAACGACTTTGTTAAATCCTATGAAGCACCCTCTCCCCACAGCGACCCGGAGGC
TTTTTTTGATGCTCCCTCTTTTCAGGTTGACCTTGGTAGGGTTATGGCATCAGGCGGGTTTGATCGGCTCTTTCTCAACT
CCTTCGGGGCAGGGCTTACCGGGCGGATTGCCAGAAGGGTCCGCCAGACCCGATGGCTGCGGGGAGACGTGGTGTATCTC
TATGCTCTCTTGAGGGAGCTTTTAGGTTATACGGCGTTAAAAATGCATATTAAGCTCATTACAGAGGGAGAAACTCTTGT
GCTTGACGAGCCGGTTTTTGCTTTTTCTGTTGGAAATGGCAGGGTGGAAGGCGGTCGGTTTCATATTGCTCCGGATGCCG
ATATTACTGACGGTCTTCTGGATGTCTGCATTCTCAAATCGATATCGAGAATACGTTTTCTTCCCTATGTCTTCAAGTAT
ATGAGCGGCAGTCAGGTTAACGATCCCCGGGTTGTCTACAAAAAAGTCTCTGCAGTCGAGCTTGCTTTTGAAAGCACTGA
AGTAATACACATGGACGGAGAAGTCTTTGAAGATCTCTCCGGAACGATTCGTATTGACGCTGCACCCCGTGCCCTGAACC
TTCTTGGATATCAGGGAGTTCGTTAA

Upstream 100 bases:

>100_bases
TCGGGCCATAGGGTATGCGTACAGGCAGATTCTCGGGGGTTTGCATAGCTCGGAATCGTCTGACATCAATGGATTTTCAT
GATGATGATGGAGTAATTGT

Downstream 100 bases:

>100_bases
GAGTAGATTCTTCAACGCCTATTGCTCTGTTTATCCTGTTTCCTGTCATTCGTCACTGAAGCCATGCAGATAGTCATATT
TGAAGACGAGACCGCATACT

Product: diacylglycerol kinase catalytic region

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKYTFIVNPVAGSRRLFAPATDMLKTLCRTRSADIVETRHAGHAGELAEESLRNGDVVIACGGDGTAHEVANILAFSERI
MGVFPAGSANDFVKSYEAPSPHSDPEAFFDAPSFQVDLGRVMASGGFDRLFLNSFGAGLTGRIARRVRQTRWLRGDVVYL
YALLRELLGYTALKMHIKLITEGETLVLDEPVFAFSVGNGRVEGGRFHIAPDADITDGLLDVCILKSISRIRFLPYVFKY
MSGSQVNDPRVVYKKVSAVELAFESTEVIHMDGEVFEDLSGTIRIDAAPRALNLLGYQGVR

Sequences:

>Translated_301_residues
MKYTFIVNPVAGSRRLFAPATDMLKTLCRTRSADIVETRHAGHAGELAEESLRNGDVVIACGGDGTAHEVANILAFSERI
MGVFPAGSANDFVKSYEAPSPHSDPEAFFDAPSFQVDLGRVMASGGFDRLFLNSFGAGLTGRIARRVRQTRWLRGDVVYL
YALLRELLGYTALKMHIKLITEGETLVLDEPVFAFSVGNGRVEGGRFHIAPDADITDGLLDVCILKSISRIRFLPYVFKY
MSGSQVNDPRVVYKKVSAVELAFESTEVIHMDGEVFEDLSGTIRIDAAPRALNLLGYQGVR
>Mature_301_residues
MKYTFIVNPVAGSRRLFAPATDMLKTLCRTRSADIVETRHAGHAGELAEESLRNGDVVIACGGDGTAHEVANILAFSERI
MGVFPAGSANDFVKSYEAPSPHSDPEAFFDAPSFQVDLGRVMASGGFDRLFLNSFGAGLTGRIARRVRQTRWLRGDVVYL
YALLRELLGYTALKMHIKLITEGETLVLDEPVFAFSVGNGRVEGGRFHIAPDADITDGLLDVCILKSISRIRFLPYVFKY
MSGSQVNDPRVVYKKVSAVELAFESTEVIHMDGEVFEDLSGTIRIDAAPRALNLLGYQGVR

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derive

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Secreted, cell wall (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=262, Percent_Identity=26.7175572519084, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 33011; Mature: 33011

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTFIVNPVAGSRRLFAPATDMLKTLCRTRSADIVETRHAGHAGELAEESLRNGDVVIA
CEEEEEEECCCCCCEEECCHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHCCCCEEEE
CGGDGTAHEVANILAFSERIMGVFPAGSANDFVKSYEAPSPHSDPEAFFDAPSFQVDLGR
ECCCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCCCCCHHHHCCCCCEEHHHH
VMASGGFDRLFLNSFGAGLTGRIARRVRQTRWLRGDVVYLYALLRELLGYTALKMHIKLI
HHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHEEEEEEEEE
TEGETLVLDEPVFAFSVGNGRVEGGRFHIAPDADITDGLLDVCILKSISRIRFLPYVFKY
ECCCEEEEECCEEEEECCCCEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MSGSQVNDPRVVYKKVSAVELAFESTEVIHMDGEVFEDLSGTIRIDAAPRALNLLGYQGV
HCCCCCCCCHHHHHHHHHHHEEECCCEEEEECCHHHHCCCCEEEEECCCHHHHHCCCCCC
R
C
>Mature Secondary Structure
MKYTFIVNPVAGSRRLFAPATDMLKTLCRTRSADIVETRHAGHAGELAEESLRNGDVVIA
CEEEEEEECCCCCCEEECCHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHCCCCEEEE
CGGDGTAHEVANILAFSERIMGVFPAGSANDFVKSYEAPSPHSDPEAFFDAPSFQVDLGR
ECCCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCCCCCHHHHCCCCCEEHHHH
VMASGGFDRLFLNSFGAGLTGRIARRVRQTRWLRGDVVYLYALLRELLGYTALKMHIKLI
HHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHEEEEEEEEE
TEGETLVLDEPVFAFSVGNGRVEGGRFHIAPDADITDGLLDVCILKSISRIRFLPYVFKY
ECCCEEEEECCEEEEECCCCEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MSGSQVNDPRVVYKKVSAVELAFESTEVIHMDGEVFEDLSGTIRIDAAPRALNLLGYQGV
HCCCCCCCCHHHHHHHHHHHEEECCCEEEEECCHHHHCCCCEEEEECCCHHHHHCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]