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The map label for this gene is leuA [H]

Identifier: 194333573

GI number: 194333573

Start: 788975

End: 790549

Strand: Reverse

Name: leuA [H]

Synonym: Paes_0739

Alternate gene names: 194333573

Gene position: 790549-788975 (Counterclockwise)

Preceding gene: 194333574

Following gene: 194333572

Centisome position: 31.46

GC content: 49.97

Gene sequence:

>1575_bases
ATGAGCCAGAAAGAAAAGGTTCTTATATTCGATACGACATTACGCGACGGCGAGCAGTCTCCTGGGGCATCGCTCAACGT
TCAGGAAAAAATTGAGATTGCCAGGCAACTCGAAAAACTCAATGTTGATGTGATTGAGGCCGGATTTCCGGCCTCCTCGC
CGCTTCAGTTAGAGGCAGTCCGACGAGTAGGCGAAGAGTCCTCCAGAATTGTAGCGGCTCTTGCCAGAGCGGTTGAACAC
GATATCAGCACAGCGTGGAAAGCGCTTCAAAGCGCTCGACATCCTCGTATCCATACGTTTATCAGTACATCGGATATCCA
TATCATGGGCAAGTTTGGCCATGAGCGCTATGGAAAAACACTGGCTGAAAAACAAAAGACCATACTCCGGATGGCAGTCG
ATGCTGTCCTCTACGCAAAAACCTTCTGTCATGATATCGAGTTTTCGGCAGAAGATGCTGGACGCACCGATCCCGGATAT
CTTGCAGAAGTCATTGAAGCCGTTATCGATGCCGGAGCAACAACCGTCAATATTCCCGATACAACCGGCTACACGTGGCC
ATCGGAATTCGGAAAAAAAATAGCGGATCTGAAAGAAAGGGTCACCAATATTGACCAGGCGGTCATAAGCGTTCACTGCC
ACAACGATCTCGGCCTCGCCGTAGCAAATACTCTCAGCGCCATAGAAAACGGGGCACGTCAGGCAGAATGTTCTATCAAC
GGCATAGGAGAAAGAGCGGGAAACGCATCGCTTGAGGAGATCGTTATGGCCCTCAAAGTACGCAGTGACCTGCACGACTA
CTCCACCGGCATTACAACACGTGAAATATACAACACCAGCCGGATGGTATCGTCGTTCACCGGACTTATTGTACAGCCAA
ATAAAGCTATTGTAGGCGACAATGCATTTTCCCATGAGTCGGGTATCCATCAGGACGGCATGCTGAAAAACCGTCAGACC
TATGAGGTCATGACGCCTCAGTCGGTAGGAGTTCCCGAAACCCATATCGTTTTAGGGCGTCATTCCGGCAAACACGGCCT
TCAGTCAAGGCTGCTTGTGCTCGGCTACAAGGTTGAGGGCGAAGAGCTTGACAAGGTCTATCAAAGATTCCTCAGCGTTG
CCGACAAGAAAAAAGAGATTTATGACGATGATCTGAGAGTGCTCATGGGTGACGAATTCAACCGCCCTGCTGAGCCTATT
GAACTCGATTATCTGCACATCAACAGCGGGACCGCATCCATACCGACGGCAACAGTACGGATTAAAACCCGTGGCAGAAC
ATTTGAAGAATCGTCCACGGGAGACGGACCGGTTGACGCCTGCTTCAGAGCGATCGAAAGGGCTCTGAAACTCGATTCAC
TTCTCAATTCCTACTCGGTACGTTCAACCTCCGCCGGCAGGCAGGCTCTTGGAGAAGCAACGGTGCGGCTCAGGGACGGA
GATAATTTTTATACAGGGCGAGGCGTTTCCACCGATATTATTGAAGCAAGTGCAAAAGCATATCTGCAGGCACTGAGCTT
TCATGAGGATGCCTTGCAAAACGACAACAACAATTCAATCGATAACGGGATATAA

Upstream 100 bases:

>100_bases
CGCCGATATCGCTAATCCGGAAGACACTATCGTTTCAACAACAGAGATGACCGATGCTATCGTAGCAAATCTTGAGGCAT
CATAACAGGAAAGGCAGGAG

Downstream 100 bases:

>100_bases
CAGATCATGGCACAAACGATAACGCAGAAGATTCTGGCCAGAGCTGCCAATCGAAAATTTGTTAACGCAGGTGAAAATGT
CTGGCTCAACGTCGATATTC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSQKEKVLIFDTTLRDGEQSPGASLNVQEKIEIARQLEKLNVDVIEAGFPASSPLQLEAVRRVGEESSRIVAALARAVEH
DISTAWKALQSARHPRIHTFISTSDIHIMGKFGHERYGKTLAEKQKTILRMAVDAVLYAKTFCHDIEFSAEDAGRTDPGY
LAEVIEAVIDAGATTVNIPDTTGYTWPSEFGKKIADLKERVTNIDQAVISVHCHNDLGLAVANTLSAIENGARQAECSIN
GIGERAGNASLEEIVMALKVRSDLHDYSTGITTREIYNTSRMVSSFTGLIVQPNKAIVGDNAFSHESGIHQDGMLKNRQT
YEVMTPQSVGVPETHIVLGRHSGKHGLQSRLLVLGYKVEGEELDKVYQRFLSVADKKKEIYDDDLRVLMGDEFNRPAEPI
ELDYLHINSGTASIPTATVRIKTRGRTFEESSTGDGPVDACFRAIERALKLDSLLNSYSVRSTSAGRQALGEATVRLRDG
DNFYTGRGVSTDIIEASAKAYLQALSFHEDALQNDNNNSIDNGI

Sequences:

>Translated_524_residues
MSQKEKVLIFDTTLRDGEQSPGASLNVQEKIEIARQLEKLNVDVIEAGFPASSPLQLEAVRRVGEESSRIVAALARAVEH
DISTAWKALQSARHPRIHTFISTSDIHIMGKFGHERYGKTLAEKQKTILRMAVDAVLYAKTFCHDIEFSAEDAGRTDPGY
LAEVIEAVIDAGATTVNIPDTTGYTWPSEFGKKIADLKERVTNIDQAVISVHCHNDLGLAVANTLSAIENGARQAECSIN
GIGERAGNASLEEIVMALKVRSDLHDYSTGITTREIYNTSRMVSSFTGLIVQPNKAIVGDNAFSHESGIHQDGMLKNRQT
YEVMTPQSVGVPETHIVLGRHSGKHGLQSRLLVLGYKVEGEELDKVYQRFLSVADKKKEIYDDDLRVLMGDEFNRPAEPI
ELDYLHINSGTASIPTATVRIKTRGRTFEESSTGDGPVDACFRAIERALKLDSLLNSYSVRSTSAGRQALGEATVRLRDG
DNFYTGRGVSTDIIEASAKAYLQALSFHEDALQNDNNNSIDNGI
>Mature_523_residues
SQKEKVLIFDTTLRDGEQSPGASLNVQEKIEIARQLEKLNVDVIEAGFPASSPLQLEAVRRVGEESSRIVAALARAVEHD
ISTAWKALQSARHPRIHTFISTSDIHIMGKFGHERYGKTLAEKQKTILRMAVDAVLYAKTFCHDIEFSAEDAGRTDPGYL
AEVIEAVIDAGATTVNIPDTTGYTWPSEFGKKIADLKERVTNIDQAVISVHCHNDLGLAVANTLSAIENGARQAECSING
IGERAGNASLEEIVMALKVRSDLHDYSTGITTREIYNTSRMVSSFTGLIVQPNKAIVGDNAFSHESGIHQDGMLKNRQTY
EVMTPQSVGVPETHIVLGRHSGKHGLQSRLLVLGYKVEGEELDKVYQRFLSVADKKKEIYDDDLRVLMGDEFNRPAEPIE
LDYLHINSGTASIPTATVRIKTRGRTFEESSTGDGPVDACFRAIERALKLDSLLNSYSVRSTSAGRQALGEATVRLRDGD
NFYTGRGVSTDIIEASAKAYLQALSFHEDALQNDNNNSIDNGI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=510, Percent_Identity=47.4509803921569, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320071, Length=387, Percent_Identity=28.4237726098191, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=387, Percent_Identity=28.1653746770026, Blast_Score=133, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=546, Percent_Identity=25.0915750915751, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324682, Length=560, Percent_Identity=24.4642857142857, Blast_Score=110, Evalue=8e-25,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57489; Mature: 57358

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKEKVLIFDTTLRDGEQSPGASLNVQEKIEIARQLEKLNVDVIEAGFPASSPLQLEAV
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHH
RRVGEESSRIVAALARAVEHDISTAWKALQSARHPRIHTFISTSDIHIMGKFGHERYGKT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCHHHHHHH
LAEKQKTILRMAVDAVLYAKTFCHDIEFSAEDAGRTDPGYLAEVIEAVIDAGATTVNIPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCC
TTGYTWPSEFGKKIADLKERVTNIDQAVISVHCHNDLGLAVANTLSAIENGARQAECSIN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
GIGERAGNASLEEIVMALKVRSDLHDYSTGITTREIYNTSRMVSSFTGLIVQPNKAIVGD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECCCCEEECC
NAFSHESGIHQDGMLKNRQTYEVMTPQSVGVPETHIVLGRHSGKHGLQSRLLVLGYKVEG
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCHHHCCEEEEEEEECH
EELDKVYQRFLSVADKKKEIYDDDLRVLMGDEFNRPAEPIELDYLHINSGTASIPTATVR
HHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCEEEEEEEECCCCCCCCEEEEE
IKTRGRTFEESSTGDGPVDACFRAIERALKLDSLLNSYSVRSTSAGRQALGEATVRLRDG
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEECC
DNFYTGRGVSTDIIEASAKAYLQALSFHEDALQNDNNNSIDNGI
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SQKEKVLIFDTTLRDGEQSPGASLNVQEKIEIARQLEKLNVDVIEAGFPASSPLQLEAV
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHH
RRVGEESSRIVAALARAVEHDISTAWKALQSARHPRIHTFISTSDIHIMGKFGHERYGKT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCHHHHHHH
LAEKQKTILRMAVDAVLYAKTFCHDIEFSAEDAGRTDPGYLAEVIEAVIDAGATTVNIPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCC
TTGYTWPSEFGKKIADLKERVTNIDQAVISVHCHNDLGLAVANTLSAIENGARQAECSIN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
GIGERAGNASLEEIVMALKVRSDLHDYSTGITTREIYNTSRMVSSFTGLIVQPNKAIVGD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECCCCEEECC
NAFSHESGIHQDGMLKNRQTYEVMTPQSVGVPETHIVLGRHSGKHGLQSRLLVLGYKVEG
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCHHHCCEEEEEEEECH
EELDKVYQRFLSVADKKKEIYDDDLRVLMGDEFNRPAEPIELDYLHINSGTASIPTATVR
HHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCEEEEEEEECCCCCCCCEEEEE
IKTRGRTFEESSTGDGPVDACFRAIERALKLDSLLNSYSVRSTSAGRQALGEATVRLRDG
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEECC
DNFYTGRGVSTDIIEASAKAYLQALSFHEDALQNDNNNSIDNGI
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA