Click here to switch to the map view.

The map label for this gene is leuB [H]

Identifier: 194333574

GI number: 194333574

Start: 790565

End: 791626

Strand: Reverse

Name: leuB [H]

Synonym: Paes_0740

Alternate gene names: 194333574

Gene position: 791626-790565 (Counterclockwise)

Preceding gene: 194333575

Following gene: 194333573

Centisome position: 31.5

GC content: 50.66

Gene sequence:

>1062_bases
ATGTTCAAGATTGTCTCTATACCCGGTGACGGGATTGGACCGGAAGTGGTGGCAAGTGCCATCCGGGTCATCAATACGCT
TGCCCGACAGCATGGCCTTGAAATTACAATCGAGGAGCACCTTTTCGGAGGTGCCTCCTATGATGTTCACGGTGAGATGC
TGACCCAGGAAACCCTCGAAGCCTGCCGCAAATGCGATGCGGTTCTCCTTGGTGCGGTCGGTGGACCGAAATGGGAAAAT
CTGCCTCATGAGAAAAAACCCGAAGCGGCCCTCCTCGGGATCCGCAAGGAACTCGGTCTGTTTGCCAATTTCAGACCAGC
CAAAGTCTACGATGCGCTGGTCAATGCGTCATCACTCAAACCTGAGATTCTTGCAGGCACCGATTTCATGGTCATCAGAG
AACTGACCGGCGGAATCTACTTCGGAGAGCCAAGAGGTTATGACGACACCAGAGGCTGGAATACGATGGTCTATGAGCAC
TATGAAGTCGAGCGCATTGCCCGTCTTGCCTTTGATGCAGCCATGAAACGCAAGAAACAACTTGTCTCAATAGATAAAGC
CAACGTTCTCGAAAGCTCTCAGTTCTGGAGAAATATTGTTCATGAAGTCCATAAGGACTTTCCTGAGGTCGAACTCATCG
ACATGTATGTCGACAACGCGGCCATGCAGATAGTACGCGATCCCAAACAGTTTGACGTTATCGTCACAAAAAACCTGTTT
GGCGACATTCTCAGCGATATCTCCGGCATGATCACCGGAAGCCTCGGCATGCTTCCTTCGGCAAGTATCGGCTCAAGCCA
TGCGCTCTATGAGCCTATTCACGGCAGCGCCCCGGACATCGCAGGGCAGAACAAAGCCAACCCGATAGCTACGATCGCAT
CGGCGGCCATGATGTTCGAACACAGCTTCTGTATGAAAAATGTCGCAGATGAAATATACAGCGCCATTGAAAAAACACTG
GCTTCGGGATTCAGAACCGCCGATATCGCTAATCCGGAAGACACTATCGTTTCAACAACAGAGATGACCGATGCTATCGT
AGCAAATCTTGAGGCATCATAA

Upstream 100 bases:

>100_bases
ACCAGCCTTCGCGAATCAAATACGAACCACCCCATCGAGAAAGTCGGGGCAAAACTCCGCAACATGATGAGCTGGCTCAT
TAAAAAATAAGTGATCAAGC

Downstream 100 bases:

>100_bases
CAGGAAAGGCAGGAGATGAGCCAGAAAGAAAAGGTTCTTATATTCGATACGACATTACGCGACGGCGAGCAGTCTCCTGG
GGCATCGCTCAACGTTCAGG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MFKIVSIPGDGIGPEVVASAIRVINTLARQHGLEITIEEHLFGGASYDVHGEMLTQETLEACRKCDAVLLGAVGGPKWEN
LPHEKKPEAALLGIRKELGLFANFRPAKVYDALVNASSLKPEILAGTDFMVIRELTGGIYFGEPRGYDDTRGWNTMVYEH
YEVERIARLAFDAAMKRKKQLVSIDKANVLESSQFWRNIVHEVHKDFPEVELIDMYVDNAAMQIVRDPKQFDVIVTKNLF
GDILSDISGMITGSLGMLPSASIGSSHALYEPIHGSAPDIAGQNKANPIATIASAAMMFEHSFCMKNVADEIYSAIEKTL
ASGFRTADIANPEDTIVSTTEMTDAIVANLEAS

Sequences:

>Translated_353_residues
MFKIVSIPGDGIGPEVVASAIRVINTLARQHGLEITIEEHLFGGASYDVHGEMLTQETLEACRKCDAVLLGAVGGPKWEN
LPHEKKPEAALLGIRKELGLFANFRPAKVYDALVNASSLKPEILAGTDFMVIRELTGGIYFGEPRGYDDTRGWNTMVYEH
YEVERIARLAFDAAMKRKKQLVSIDKANVLESSQFWRNIVHEVHKDFPEVELIDMYVDNAAMQIVRDPKQFDVIVTKNLF
GDILSDISGMITGSLGMLPSASIGSSHALYEPIHGSAPDIAGQNKANPIATIASAAMMFEHSFCMKNVADEIYSAIEKTL
ASGFRTADIANPEDTIVSTTEMTDAIVANLEAS
>Mature_353_residues
MFKIVSIPGDGIGPEVVASAIRVINTLARQHGLEITIEEHLFGGASYDVHGEMLTQETLEACRKCDAVLLGAVGGPKWEN
LPHEKKPEAALLGIRKELGLFANFRPAKVYDALVNASSLKPEILAGTDFMVIRELTGGIYFGEPRGYDDTRGWNTMVYEH
YEVERIARLAFDAAMKRKKQLVSIDKANVLESSQFWRNIVHEVHKDFPEVELIDMYVDNAAMQIVRDPKQFDVIVTKNLF
GDILSDISGMITGSLGMLPSASIGSSHALYEPIHGSAPDIAGQNKANPIATIASAAMMFEHSFCMKNVADEIYSAIEKTL
ASGFRTADIANPEDTIVSTTEMTDAIVANLEAS

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=301, Percent_Identity=31.8936877076412, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI28178816, Length=308, Percent_Identity=27.9220779220779, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI28178821, Length=309, Percent_Identity=27.831715210356, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4758582, Length=304, Percent_Identity=27.9605263157895, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI28178838, Length=304, Percent_Identity=27.9605263157895, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI28178819, Length=142, Percent_Identity=33.8028169014084, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI87081683, Length=352, Percent_Identity=50.2840909090909, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1788101, Length=364, Percent_Identity=34.3406593406593, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1787381, Length=400, Percent_Identity=26.25, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71986051, Length=353, Percent_Identity=29.7450424929178, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17550882, Length=351, Percent_Identity=27.3504273504274, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=364, Percent_Identity=28.2967032967033, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17505779, Length=354, Percent_Identity=26.8361581920904, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=46.0893854748603, Blast_Score=287, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6322097, Length=362, Percent_Identity=35.6353591160221, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324709, Length=356, Percent_Identity=32.5842696629214, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6324291, Length=361, Percent_Identity=31.5789473684211, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24643270, Length=359, Percent_Identity=30.9192200557103, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24643268, Length=359, Percent_Identity=30.9192200557103, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24661184, Length=361, Percent_Identity=27.7008310249307, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078637, Length=356, Percent_Identity=28.3707865168539, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161078635, Length=356, Percent_Identity=28.3707865168539, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161078633, Length=356, Percent_Identity=28.3707865168539, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24650122, Length=356, Percent_Identity=28.3707865168539, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI161078639, Length=353, Percent_Identity=28.6118980169972, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI281362242, Length=354, Percent_Identity=27.1186440677966, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648872, Length=354, Percent_Identity=27.1186440677966, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20130355, Length=334, Percent_Identity=24.8502994011976, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38618; Mature: 38618

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIVSIPGDGIGPEVVASAIRVINTLARQHGLEITIEEHLFGGASYDVHGEMLTQETLE
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCHHHHHHHHHH
ACRKCDAVLLGAVGGPKWENLPHEKKPEAALLGIRKELGLFANFRPAKVYDALVNASSLK
HHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
PEILAGTDFMVIRELTGGIYFGEPRGYDDTRGWNTMVYEHYEVERIARLAFDAAMKRKKQ
CCEECCCCHHHHHHHHCCEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LVSIDKANVLESSQFWRNIVHEVHKDFPEVELIDMYVDNAAMQIVRDPKQFDVIVTKNLF
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHEEEEEHHHH
GDILSDISGMITGSLGMLPSASIGSSHALYEPIHGSAPDIAGQNKANPIATIASAAMMFE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HSFCMKNVADEIYSAIEKTLASGFRTADIANPEDTIVSTTEMTDAIVANLEAS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFKIVSIPGDGIGPEVVASAIRVINTLARQHGLEITIEEHLFGGASYDVHGEMLTQETLE
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCHHHHHHHHHH
ACRKCDAVLLGAVGGPKWENLPHEKKPEAALLGIRKELGLFANFRPAKVYDALVNASSLK
HHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
PEILAGTDFMVIRELTGGIYFGEPRGYDDTRGWNTMVYEHYEVERIARLAFDAAMKRKKQ
CCEECCCCHHHHHHHHCCEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LVSIDKANVLESSQFWRNIVHEVHKDFPEVELIDMYVDNAAMQIVRDPKQFDVIVTKNLF
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHEEEEEHHHH
GDILSDISGMITGSLGMLPSASIGSSHALYEPIHGSAPDIAGQNKANPIATIASAAMMFE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HSFCMKNVADEIYSAIEKTLASGFRTADIANPEDTIVSTTEMTDAIVANLEAS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]