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The map label for this gene is leuC [H]

Identifier: 194333572

GI number: 194333572

Start: 787670

End: 788968

Strand: Reverse

Name: leuC [H]

Synonym: Paes_0738

Alternate gene names: 194333572

Gene position: 788968-787670 (Counterclockwise)

Preceding gene: 194333573

Following gene: 194333571

Centisome position: 31.4

GC content: 51.89

Gene sequence:

>1299_bases
ATGGCACAAACGATAACGCAGAAGATTCTGGCCAGAGCTGCCAATCGAAAATTTGTTAACGCAGGTGAAAATGTCTGGCT
CAACGTCGATATTCTCCTTACGCATGACGTATGCGGACCTCCGACCTTCGATATTTTCAAGGAAAAATTCGGCCCGGACG
CCAAAGTATGGGATCCTGAAAAAGTCGTTGTACTACCCGACCACTACATTTTCACAGAAAACGAACACGCACACCGTAAT
ATCGATCTTCTGCGCACTTTCGCTGCAGAACAGAACCTCCCCAACTATTACGATGTCGGTACCGATCGTTACAAAGGAGT
CTGCCATGTTGCGCTTGCACAGGAAGGCTTCAACCTTCCCGGCACCGTCCTCTTCGGGACCGATTCCCATACCTGTACAT
CGGGTGCGTTCGGCATGTTCGGGACCGGTATCGGCAACACCGATGCCGCCTTCATCCTGGGAACAGGAAAACTCTGGGAA
AAAGTCCCTGAATCAATGAAATTCGTTTTTGACGGTGCCATGCCGGACTATCTCACCGCCAAAGACCTTATCCTTCAGAT
CCTCGGCGATATCGGTACCGATGGGGCGACCTACCGGGCCATGGAATTTGACGGTTCGGCAGTCTATCAGCTTCCTGTCG
AAGAGCGTATGACCCTTACCAATATGGCAATCGAAGCCGGCGGCATGAACGGCATCATTGCTGCTGATGAGGTCACAGAA
GCCTATGTCAAAGCACGAACCAGCAAACCATATGAAATCTTCCATAGCGACCCGGATGCCATCTATCACAGCATCTACCA
CTATAATGTTGAAGAACTCGAGCCTGTCGTAGCCCAGCCGCACAGCCCGGACAACCGCGCAACTGTACGAAGCGTTCAGG
GCACGAGAATCACCAAATCCTACATCGGCTCATGCACCGGAGGCAAGCTTTCCGATTTCAAAATGGCTGCAAAAATTCTC
AACGGCCAGCAGGTGTCGATACAAACAAATATTGTTCCTGCAACCGTTGAAGTTGCCAAAAGTCTGGAAACAGAACAGTA
TGAAGGCAAGTCAATCCGCAAAGTCCTGGAAGATGCGGGCTGCACTATCGCACAGCCATCCTGTGCAGCATGCCTTGGCG
GCCCGGCCGATACCTTTGGGCGTTCGGAAGACAACGACCTGGTCGTATCAACGACAAACAGAAACTTCCCCGGCCGTATG
GGCAGCAAAAAAGCAGGGGTCTATCTTGCTTCGCCCCTGACGGCCGCTGCATCCGCCATTACCGGAAGGCTTACCGACCC
GAGAGAGTTTCTCTCTTAA

Upstream 100 bases:

>100_bases
TGAAGCAAGTGCAAAAGCATATCTGCAGGCACTGAGCTTTCATGAGGATGCCTTGCAAAACGACAACAACAATTCAATCG
ATAACGGGATATAACAGATC

Downstream 100 bases:

>100_bases
GCATAGAAACAGCCTATAACGAAATCTGAAAGAACACACGATTATGGATAGCATCATACAAGGAAAAGCCTATGTGCTGG
GCAAAAACATCGATACAGAC

Product: homoaconitate hydratase family protein

Products: NA

Alternate protein names: (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MAQTITQKILARAANRKFVNAGENVWLNVDILLTHDVCGPPTFDIFKEKFGPDAKVWDPEKVVVLPDHYIFTENEHAHRN
IDLLRTFAAEQNLPNYYDVGTDRYKGVCHVALAQEGFNLPGTVLFGTDSHTCTSGAFGMFGTGIGNTDAAFILGTGKLWE
KVPESMKFVFDGAMPDYLTAKDLILQILGDIGTDGATYRAMEFDGSAVYQLPVEERMTLTNMAIEAGGMNGIIAADEVTE
AYVKARTSKPYEIFHSDPDAIYHSIYHYNVEELEPVVAQPHSPDNRATVRSVQGTRITKSYIGSCTGGKLSDFKMAAKIL
NGQQVSIQTNIVPATVEVAKSLETEQYEGKSIRKVLEDAGCTIAQPSCAACLGGPADTFGRSEDNDLVVSTTNRNFPGRM
GSKKAGVYLASPLTAAASAITGRLTDPREFLS

Sequences:

>Translated_432_residues
MAQTITQKILARAANRKFVNAGENVWLNVDILLTHDVCGPPTFDIFKEKFGPDAKVWDPEKVVVLPDHYIFTENEHAHRN
IDLLRTFAAEQNLPNYYDVGTDRYKGVCHVALAQEGFNLPGTVLFGTDSHTCTSGAFGMFGTGIGNTDAAFILGTGKLWE
KVPESMKFVFDGAMPDYLTAKDLILQILGDIGTDGATYRAMEFDGSAVYQLPVEERMTLTNMAIEAGGMNGIIAADEVTE
AYVKARTSKPYEIFHSDPDAIYHSIYHYNVEELEPVVAQPHSPDNRATVRSVQGTRITKSYIGSCTGGKLSDFKMAAKIL
NGQQVSIQTNIVPATVEVAKSLETEQYEGKSIRKVLEDAGCTIAQPSCAACLGGPADTFGRSEDNDLVVSTTNRNFPGRM
GSKKAGVYLASPLTAAASAITGRLTDPREFLS
>Mature_431_residues
AQTITQKILARAANRKFVNAGENVWLNVDILLTHDVCGPPTFDIFKEKFGPDAKVWDPEKVVVLPDHYIFTENEHAHRNI
DLLRTFAAEQNLPNYYDVGTDRYKGVCHVALAQEGFNLPGTVLFGTDSHTCTSGAFGMFGTGIGNTDAAFILGTGKLWEK
VPESMKFVFDGAMPDYLTAKDLILQILGDIGTDGATYRAMEFDGSAVYQLPVEERMTLTNMAIEAGGMNGIIAADEVTEA
YVKARTSKPYEIFHSDPDAIYHSIYHYNVEELEPVVAQPHSPDNRATVRSVQGTRITKSYIGSCTGGKLSDFKMAAKILN
GQQVSIQTNIVPATVEVAKSLETEQYEGKSIRKVLEDAGCTIAQPSCAACLGGPADTFGRSEDNDLVVSTTNRNFPGRMG
SKKAGVYLASPLTAAASAITGRLTDPREFLS

Specific function: Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate, a step involved

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=375, Percent_Identity=24.8, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI8659555, Length=383, Percent_Identity=22.7154046997389, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI41352693, Length=382, Percent_Identity=21.9895287958115, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1786259, Length=478, Percent_Identity=30.3347280334728, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI2367097, Length=478, Percent_Identity=25.9414225941423, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787531, Length=357, Percent_Identity=23.249299719888, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI32564738, Length=389, Percent_Identity=25.4498714652956, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=389, Percent_Identity=25.4498714652956, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17568399, Length=380, Percent_Identity=24.7368421052632, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25149342, Length=290, Percent_Identity=25.5172413793103, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=459, Percent_Identity=30.5010893246187, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320440, Length=460, Percent_Identity=30, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323335, Length=368, Percent_Identity=25.2717391304348, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322261, Length=392, Percent_Identity=23.7244897959184, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28571643, Length=365, Percent_Identity=26.5753424657534, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161076999, Length=342, Percent_Identity=25.1461988304094, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281365315, Length=342, Percent_Identity=25.1461988304094, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17864292, Length=342, Percent_Identity=25.1461988304094, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137564, Length=367, Percent_Identity=25.8855585831063, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33; =4.2.1.35; =4.2.1.31 [H]

Molecular weight: Translated: 46957; Mature: 46826

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTITQKILARAANRKFVNAGENVWLNVDILLTHDVCGPPTFDIFKEKFGPDAKVWDPE
CCHHHHHHHHHHHHCCCEECCCCEEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCC
KVVVLPDHYIFTENEHAHRNIDLLRTFAAEQNLPNYYDVGTDRYKGVCHVALAQEGFNLP
EEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEEECCCCCCC
GTVLFGTDSHTCTSGAFGMFGTGIGNTDAAFILGTGKLWEKVPESMKFVFDGAMPDYLTA
CEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHEEEECCCCCCHHHH
KDLILQILGDIGTDGATYRAMEFDGSAVYQLPVEERMTLTNMAIEAGGMNGIIAADEVTE
HHHHHHHHHHCCCCCCEEEEEEECCCEEEECCHHHHHHHHHHEEECCCCCCEEEHHHHHH
AYVKARTSKPYEIFHSDPDAIYHSIYHYNVEELEPVVAQPHSPDNRATVRSVQGTRITKS
HHHHHCCCCCEEEECCCHHHHHHHHHHCCHHHHCHHHCCCCCCCCCCEEEECCCCEEEHH
YIGSCTGGKLSDFKMAAKILNGQQVSIQTNIVPATVEVAKSLETEQYEGKSIRKVLEDAG
HHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHCCCHHHHHHHHHCC
CTIAQPSCAACLGGPADTFGRSEDNDLVVSTTNRNFPGRMGSKKAGVYLASPLTAAASAI
CEECCCCHHHHCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHH
TGRLTDPREFLS
HCCCCCHHHHHC
>Mature Secondary Structure 
AQTITQKILARAANRKFVNAGENVWLNVDILLTHDVCGPPTFDIFKEKFGPDAKVWDPE
CHHHHHHHHHHHHCCCEECCCCEEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCC
KVVVLPDHYIFTENEHAHRNIDLLRTFAAEQNLPNYYDVGTDRYKGVCHVALAQEGFNLP
EEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEEECCCCCCC
GTVLFGTDSHTCTSGAFGMFGTGIGNTDAAFILGTGKLWEKVPESMKFVFDGAMPDYLTA
CEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHEEEECCCCCCHHHH
KDLILQILGDIGTDGATYRAMEFDGSAVYQLPVEERMTLTNMAIEAGGMNGIIAADEVTE
HHHHHHHHHHCCCCCCEEEEEEECCCEEEECCHHHHHHHHHHEEECCCCCCEEEHHHHHH
AYVKARTSKPYEIFHSDPDAIYHSIYHYNVEELEPVVAQPHSPDNRATVRSVQGTRITKS
HHHHHCCCCCEEEECCCHHHHHHHHHHCCHHHHCHHHCCCCCCCCCCEEEECCCCEEEHH
YIGSCTGGKLSDFKMAAKILNGQQVSIQTNIVPATVEVAKSLETEQYEGKSIRKVLEDAG
HHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHCCCHHHHHHHHHCC
CTIAQPSCAACLGGPADTFGRSEDNDLVVSTTNRNFPGRMGSKKAGVYLASPLTAAASAI
CEECCCCHHHHCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHH
TGRLTDPREFLS
HCCCCCHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]