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The map label for this gene is leuD1 [H]

Identifier: 194333571

GI number: 194333571

Start: 787066

End: 787626

Strand: Reverse

Name: leuD1 [H]

Synonym: Paes_0737

Alternate gene names: 194333571

Gene position: 787626-787066 (Counterclockwise)

Preceding gene: 194333572

Following gene: 194333570

Centisome position: 31.34

GC content: 50.09

Gene sequence:

>561_bases
ATGGATAGCATCATACAAGGAAAAGCCTATGTGCTGGGCAAAAACATCGATACAGACCAGATCATTCCTGCCGAACACCT
CGTCTACAGTCTCTCAGACCCTGAAGAGGTCAAGCTCTATGGCAAATACGCCCTTTCCGGCGTACCGGTTGCCGAAGCAG
GTCTTCCGGAAGGAAACACTCCCTTTGTCGCAGACAGCAGTTTCGAGTCTGAATACGTGATCATCATCGCGGGTCCGAAT
TTCGGTTGTGGCTCATCTCGTGAACATGCGCCCTTTGCACTCCAGGTTGCCGGCGTCAAGGCAATTGTCGCAGAATCCTA
TGCCCGAATTTTTTACCGCAACTGCGTCGATGGCGGATTTGTGATCCCTTATGAGACTTCAGAACAGCTCAATAAATCCG
TTCTGACAGGTGACGAACTGAAAATCGACGTCCAGAACAATACGATTACCAACCTGACCCAGAATATCACCTATACCCTG
TCGCCGCTCGGTGATGTTCTTGAAATCGTCAAGGCAGGCGGCATTTTTGCCTATGCCAGGCAGGAAAACCTTATGGCTTG
A

Upstream 100 bases:

>100_bases
GCCGCTGCATCCGCCATTACCGGAAGGCTTACCGACCCGAGAGAGTTTCTCTCTTAAGCATAGAAACAGCCTATAACGAA
ATCTGAAAGAACACACGATT

Downstream 100 bases:

>100_bases
ACTCGATATGAAGGAAAGAAACGCAATCGAACTCTACGATACAACGCTCCGGGACGGAACCCAGGGTGAAAAAATCAACC
TCTCCGTACAAGATAAGCTG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MDSIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPEEVKLYGKYALSGVPVAEAGLPEGNTPFVADSSFESEYVIIIAGPN
FGCGSSREHAPFALQVAGVKAIVAESYARIFYRNCVDGGFVIPYETSEQLNKSVLTGDELKIDVQNNTITNLTQNITYTL
SPLGDVLEIVKAGGIFAYARQENLMA

Sequences:

>Translated_186_residues
MDSIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPEEVKLYGKYALSGVPVAEAGLPEGNTPFVADSSFESEYVIIIAGPN
FGCGSSREHAPFALQVAGVKAIVAESYARIFYRNCVDGGFVIPYETSEQLNKSVLTGDELKIDVQNNTITNLTQNITYTL
SPLGDVLEIVKAGGIFAYARQENLMA
>Mature_186_residues
MDSIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPEEVKLYGKYALSGVPVAEAGLPEGNTPFVADSSFESEYVIIIAGPN
FGCGSSREHAPFALQVAGVKAIVAESYARIFYRNCVDGGFVIPYETSEQLNKSVLTGDELKIDVQNNTITNLTQNITYTL
SPLGDVLEIVKAGGIFAYARQENLMA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=114, Percent_Identity=35.9649122807018, Blast_Score=63, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321429, Length=148, Percent_Identity=31.7567567567568, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 20086; Mature: 20086

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPEEVKLYGKYALSGVPVAEAGLPEGNT
CCCCCCCCEEEEECCCCCCCCCCHHHEEEECCCCHHEEEEEEEEECCCCCHHCCCCCCCC
PFVADSSFESEYVIIIAGPNFGCGSSREHAPFALQVAGVKAIVAESYARIFYRNCVDGGF
CEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCE
VIPYETSEQLNKSVLTGDELKIDVQNNTITNLTQNITYTLSPLGDVLEIVKAGGIFAYAR
EEEECCHHHHHHHHCCCCEEEEEECCCEEEEEECCEEEEECCHHHHHHHHHCCCEEEEEC
QENLMA
CCCCCC
>Mature Secondary Structure
MDSIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPEEVKLYGKYALSGVPVAEAGLPEGNT
CCCCCCCCEEEEECCCCCCCCCCHHHEEEECCCCHHEEEEEEEEECCCCCHHCCCCCCCC
PFVADSSFESEYVIIIAGPNFGCGSSREHAPFALQVAGVKAIVAESYARIFYRNCVDGGF
CEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCE
VIPYETSEQLNKSVLTGDELKIDVQNNTITNLTQNITYTLSPLGDVLEIVKAGGIFAYAR
EEEECCHHHHHHHHCCCCEEEEEECCCEEEEEECCEEEEECCHHHHHHHHHCCCEEEEEC
QENLMA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]