Click here to switch to the map view.

The map label for this gene is pnp

Identifier: 194333349

GI number: 194333349

Start: 547133

End: 549337

Strand: Reverse

Name: pnp

Synonym: Paes_0506

Alternate gene names: 194333349

Gene position: 549337-547133 (Counterclockwise)

Preceding gene: 194333350

Following gene: 194333348

Centisome position: 21.86

GC content: 52.61

Gene sequence:

>2205_bases
ATGTCCATGTTTATACGAAAAGAAATTGATCTGGGAAGCGGGAAAACACTCTCTATCGAGACAGGGAAAATGGCAAAACA
GGCCGATGGCTCGGCCATTGTTCGCCTGAATGACACTATGGTGCTGGCTACCGTGGTTTCAAGCAAAACCCCTCCTTCCC
CGAACCAGAGCTTTTTTCCTCTGCAGGTTGAGTACCGTGAGAAATATTCCGCTGCCGGAAAATTCCCCGGAGGCTTTTTC
AAACGTGAAGGCAGGCCTTCTGAGAAAGAGATTCTTTCAGCACGTCTTATAGATCGCGCTCTTCGTCCGCTCTTCCCTGA
CGGGTATTATCAGGACACTCAGATTATCATTTCGGTTATATCCTCTGACCAGATCAACGATGCCGATGTTCTTGGCGGGG
TAGCTGCTTCAGCAGCGATCATGGTCTCCGACATCCCCTTTCAGAACTCGATGTCCGAAGTTCGGGTAGGAAGAGTCAAT
GGCGAATATATCGTCAACCCCAACATCAACGAACTGCGCGACAGTGACATTGATATCAGTATCGGCGGAACGGAGAATAC
GATCTGCATGCTGGAAGGTGAGATGGACGAAATTTCAGAGGCTGAAATGCTGGAGGCGATCCGTTTCGGTCATGAGGCCA
TCAAAAAGATCTGCGCTCTTCAGAATGAGATCGCAGCCGAAGTCGGCAAAACGGCGCGCACCTTCAGCGCAGCCAAAGCT
CCCGACAACCTCCGCCAGTCGATTGCTGAAATCTGCAGCAACGAGCTCAAAGAGCTCGCATACATGCCGCTCTGCAAAGA
AGAACGGGCTGAAAAAACAGCAGAGATTTACAAAAACGCCAAGGCGCAGACCTTGCAGCGCTATCAGCAGGAAATCACTC
CCGAAGTCATTGCCGCTGAACCCGAAAAAGCGCTCTACCTGAACGAACAGATCATCGGCGACGCCATTCACTCTATCGAA
AAACAGGTTATGCGCGAGATGATCCTCGACGACGCCAAGCGTCTTGACGGAAGGCGACTCGACGAGGTTCGGCCGATCAG
CATCGAACTCGGCCTTATCCCCAGGGCACACGGCTCGGCACTCTTTACCCGCGGAGAGACGCAGGCGCTTGTCACCCTGA
CCCTGGGAACAAAGAAAGACGCCCAGATGATCGATACGCTCACCGACGACGCCGATAAACGCTTTATGCTCCACTATAAC
TTCCCTCCGTTCTCCGTCGGAGAAACAGGAAGAGTCGGCGGCACCAGCCGAAGGGAAATCGGCCATGGCAACCTTGCTGA
AAGGGCGATCAGGAAAGTGGCCCCAGCCGAATCGGCATTCCCGTATACCATCCGTATCGTTTCCGATATTCTTGAATCAA
ACGGTTCATCCTCTATGGCTTCGGTCTGTGGCGGCGCTCTGGCAGCTATGGATGGCGGTGTACCGCTCAGAAAACCGGTC
TCCGGCATCGCTATGGGTCTGATCAAGGAAGGCGACAGATATGCTGTCCTCTCCGACATTCTCGGCAATGAAGACCATCT
TGGCGATATGGACTTCAAAGTAGCAGGTACAGCCGATGGCATTACCGCTTGCCAGATGGATATCAAGATCGATGGTCTGG
ATTACCACATTCTTGAACAGGCGCTCGAACAGGCGCTGCATGGCAGATTGCACATTCTCGACAAAATGAACGAGGCAATC
CAGGAGCCGAGAACCGAGATCGGCAAATACGCTCCAAAGCTCACCACCATTCAGATTCCTGTCGATGCTATCGGTATGGT
CATCGGTAAAGGCGGAGAAACGATCCGCAGCATCACCGAAGAGACCGGTGCTGAAATCAACATCGAAGACGACGGGACTG
TTACCATCGCTTCGGCCAGTGGCGAAGGCGCTTCGGCTGCACTTGAAACGATCAAACTGCTCATTTCAAAACCTGAAGTC
GGCACTGTCTACTCCGGTAAAGTCCGTGATATCAGGGAAGATCTTGGTGCATTTGTCGAGTTTCTGCCGAAGACAGATGG
TCTGGTTCATATTTCCGAAATTTCCAATGAAAGGGTCGCCAAAGTCAGCGACCACCTGAAACCCGGCGACAAAGTCAAAG
TCAAGCTTGTCGATGTCCGCAAGGATCCCCGCACCGGCAAAACCCGTTTTGCGCTTTCCATCAAGGCGCTCGCGGAAAAA
TCAGCCGATAACGGAGCGAGCGACAAGGCGGAAGCCAACCGGTAA

Upstream 100 bases:

>100_bases
CATACCCGTCCGTCACCTTCAGATCCAGCGGCAGACTGTACGCTACAAGGATCCGGGAAATACGAAGATATTGTTCAAGC
CTTTTGTTCACCAACTATCG

Downstream 100 bases:

>100_bases
ACGACCGTCAACGTCCATGACTATCAAGGAAAAGCTGTGCAGAACTGACTGCACGGCTTTTTTCTTGCCCGCTCGCACAA
AGGCGCTACATTGCTATAAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 734; Mature: 733

Protein sequence:

>734_residues
MSMFIRKEIDLGSGKTLSIETGKMAKQADGSAIVRLNDTMVLATVVSSKTPPSPNQSFFPLQVEYREKYSAAGKFPGGFF
KREGRPSEKEILSARLIDRALRPLFPDGYYQDTQIIISVISSDQINDADVLGGVAASAAIMVSDIPFQNSMSEVRVGRVN
GEYIVNPNINELRDSDIDISIGGTENTICMLEGEMDEISEAEMLEAIRFGHEAIKKICALQNEIAAEVGKTARTFSAAKA
PDNLRQSIAEICSNELKELAYMPLCKEERAEKTAEIYKNAKAQTLQRYQQEITPEVIAAEPEKALYLNEQIIGDAIHSIE
KQVMREMILDDAKRLDGRRLDEVRPISIELGLIPRAHGSALFTRGETQALVTLTLGTKKDAQMIDTLTDDADKRFMLHYN
FPPFSVGETGRVGGTSRREIGHGNLAERAIRKVAPAESAFPYTIRIVSDILESNGSSSMASVCGGALAAMDGGVPLRKPV
SGIAMGLIKEGDRYAVLSDILGNEDHLGDMDFKVAGTADGITACQMDIKIDGLDYHILEQALEQALHGRLHILDKMNEAI
QEPRTEIGKYAPKLTTIQIPVDAIGMVIGKGGETIRSITEETGAEINIEDDGTVTIASASGEGASAALETIKLLISKPEV
GTVYSGKVRDIREDLGAFVEFLPKTDGLVHISEISNERVAKVSDHLKPGDKVKVKLVDVRKDPRTGKTRFALSIKALAEK
SADNGASDKAEANR

Sequences:

>Translated_734_residues
MSMFIRKEIDLGSGKTLSIETGKMAKQADGSAIVRLNDTMVLATVVSSKTPPSPNQSFFPLQVEYREKYSAAGKFPGGFF
KREGRPSEKEILSARLIDRALRPLFPDGYYQDTQIIISVISSDQINDADVLGGVAASAAIMVSDIPFQNSMSEVRVGRVN
GEYIVNPNINELRDSDIDISIGGTENTICMLEGEMDEISEAEMLEAIRFGHEAIKKICALQNEIAAEVGKTARTFSAAKA
PDNLRQSIAEICSNELKELAYMPLCKEERAEKTAEIYKNAKAQTLQRYQQEITPEVIAAEPEKALYLNEQIIGDAIHSIE
KQVMREMILDDAKRLDGRRLDEVRPISIELGLIPRAHGSALFTRGETQALVTLTLGTKKDAQMIDTLTDDADKRFMLHYN
FPPFSVGETGRVGGTSRREIGHGNLAERAIRKVAPAESAFPYTIRIVSDILESNGSSSMASVCGGALAAMDGGVPLRKPV
SGIAMGLIKEGDRYAVLSDILGNEDHLGDMDFKVAGTADGITACQMDIKIDGLDYHILEQALEQALHGRLHILDKMNEAI
QEPRTEIGKYAPKLTTIQIPVDAIGMVIGKGGETIRSITEETGAEINIEDDGTVTIASASGEGASAALETIKLLISKPEV
GTVYSGKVRDIREDLGAFVEFLPKTDGLVHISEISNERVAKVSDHLKPGDKVKVKLVDVRKDPRTGKTRFALSIKALAEK
SADNGASDKAEANR
>Mature_733_residues
SMFIRKEIDLGSGKTLSIETGKMAKQADGSAIVRLNDTMVLATVVSSKTPPSPNQSFFPLQVEYREKYSAAGKFPGGFFK
REGRPSEKEILSARLIDRALRPLFPDGYYQDTQIIISVISSDQINDADVLGGVAASAAIMVSDIPFQNSMSEVRVGRVNG
EYIVNPNINELRDSDIDISIGGTENTICMLEGEMDEISEAEMLEAIRFGHEAIKKICALQNEIAAEVGKTARTFSAAKAP
DNLRQSIAEICSNELKELAYMPLCKEERAEKTAEIYKNAKAQTLQRYQQEITPEVIAAEPEKALYLNEQIIGDAIHSIEK
QVMREMILDDAKRLDGRRLDEVRPISIELGLIPRAHGSALFTRGETQALVTLTLGTKKDAQMIDTLTDDADKRFMLHYNF
PPFSVGETGRVGGTSRREIGHGNLAERAIRKVAPAESAFPYTIRIVSDILESNGSSSMASVCGGALAAMDGGVPLRKPVS
GIAMGLIKEGDRYAVLSDILGNEDHLGDMDFKVAGTADGITACQMDIKIDGLDYHILEQALEQALHGRLHILDKMNEAIQ
EPRTEIGKYAPKLTTIQIPVDAIGMVIGKGGETIRSITEETGAEINIEDDGTVTIASASGEGASAALETIKLLISKPEVG
TVYSGKVRDIREDLGAFVEFLPKTDGLVHISEISNERVAKVSDHLKPGDKVKVKLVDVRKDPRTGKTRFALSIKALAEKS
ADNGASDKAEANR

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=726, Percent_Identity=37.7410468319559, Blast_Score=459, Evalue=1e-129,
Organism=Escherichia coli, GI145693187, Length=706, Percent_Identity=48.8668555240793, Blast_Score=649, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=737, Percent_Identity=33.2428765264586, Blast_Score=341, Evalue=1e-93,
Organism=Drosophila melanogaster, GI281362905, Length=726, Percent_Identity=35.3994490358127, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=726, Percent_Identity=35.3994490358127, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=726, Percent_Identity=35.3994490358127, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=668, Percent_Identity=35.0299401197605, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_PROA2 (B4S5G5)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015209.1
- ProteinModelPortal:   B4S5G5
- SMR:   B4S5G5
- GeneID:   6459452
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_0506
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 79780; Mature: 79649

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFIRKEIDLGSGKTLSIETGKMAKQADGSAIVRLNDTMVLATVVSSKTPPSPNQSFFP
CCCEEEECCCCCCCCEEEEECCCHHHCCCCCEEEEECCCEEEEEECCCCCCCCCCCCEEE
LQVEYREKYSAAGKFPGGFFKREGRPSEKEILSARLIDRALRPLFPDGYYQDTQIIISVI
EEEHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
SSDQINDADVLGGVAASAAIMVSDIPFQNSMSEVRVGRVNGEYIVNPNINELRDSDIDIS
CCCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHEEEEECCCCEEECCCHHHHCCCCEEEE
IGGTENTICMLEGEMDEISEAEMLEAIRFGHEAIKKICALQNEIAAEVGKTARTFSAAKA
ECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
PDNLRQSIAEICSNELKELAYMPLCKEERAEKTAEIYKNAKAQTLQRYQQEITPEVIAAE
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHCCCC
PEKALYLNEQIIGDAIHSIEKQVMREMILDDAKRLDGRRLDEVRPISIELGLIPRAHGSA
CCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCE
LFTRGETQALVTLTLGTKKDAQMIDTLTDDADKRFMLHYNFPPFSVGETGRVGGTSRREI
EEECCCCEEEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
GHGNLAERAIRKVAPAESAFPYTIRIVSDILESNGSSSMASVCGGALAAMDGGVPLRKPV
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCCH
SGIAMGLIKEGDRYAVLSDILGNEDHLGDMDFKVAGTADGITACQMDIKIDGLDYHILEQ
HHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHH
ALEQALHGRLHILDKMNEAIQEPRTEIGKYAPKLTTIQIPVDAIGMVIGKGGETIRSITE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHEECCCCHHHHHHHH
ETGAEINIEDDGTVTIASASGEGASAALETIKLLISKPEVGTVYSGKVRDIREDLGAFVE
HCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHH
FLPKTDGLVHISEISNERVAKVSDHLKPGDKVKVKLVDVRKDPRTGKTRFALSIKALAEK
HHCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEEHHHHHHC
SADNGASDKAEANR
CCCCCCCCCCCCCC
>Mature Secondary Structure 
SMFIRKEIDLGSGKTLSIETGKMAKQADGSAIVRLNDTMVLATVVSSKTPPSPNQSFFP
CCEEEECCCCCCCCEEEEECCCHHHCCCCCEEEEECCCEEEEEECCCCCCCCCCCCEEE
LQVEYREKYSAAGKFPGGFFKREGRPSEKEILSARLIDRALRPLFPDGYYQDTQIIISVI
EEEHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
SSDQINDADVLGGVAASAAIMVSDIPFQNSMSEVRVGRVNGEYIVNPNINELRDSDIDIS
CCCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHEEEEECCCCEEECCCHHHHCCCCEEEE
IGGTENTICMLEGEMDEISEAEMLEAIRFGHEAIKKICALQNEIAAEVGKTARTFSAAKA
ECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
PDNLRQSIAEICSNELKELAYMPLCKEERAEKTAEIYKNAKAQTLQRYQQEITPEVIAAE
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHCCCC
PEKALYLNEQIIGDAIHSIEKQVMREMILDDAKRLDGRRLDEVRPISIELGLIPRAHGSA
CCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCE
LFTRGETQALVTLTLGTKKDAQMIDTLTDDADKRFMLHYNFPPFSVGETGRVGGTSRREI
EEECCCCEEEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
GHGNLAERAIRKVAPAESAFPYTIRIVSDILESNGSSSMASVCGGALAAMDGGVPLRKPV
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCCH
SGIAMGLIKEGDRYAVLSDILGNEDHLGDMDFKVAGTADGITACQMDIKIDGLDYHILEQ
HHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHH
ALEQALHGRLHILDKMNEAIQEPRTEIGKYAPKLTTIQIPVDAIGMVIGKGGETIRSITE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHEECCCCHHHHHHHH
ETGAEINIEDDGTVTIASASGEGASAALETIKLLISKPEVGTVYSGKVRDIREDLGAFVE
HCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHH
FLPKTDGLVHISEISNERVAKVSDHLKPGDKVKVKLVDVRKDPRTGKTRFALSIKALAEK
HHCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEEHHHHHHC
SADNGASDKAEANR
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA