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The map label for this gene is leuS

Identifier: 194333348

GI number: 194333348

Start: 544487

End: 546907

Strand: Reverse

Name: leuS

Synonym: Paes_0505

Alternate gene names: 194333348

Gene position: 546907-544487 (Counterclockwise)

Preceding gene: 194333349

Following gene: 194333347

Centisome position: 21.76

GC content: 52.79

Gene sequence:

>2421_bases
ATGCGTTACGAATTTTCAACCATCGAACAGAAATGGCAGAAATACTGGGCTGATAACCAGACCTTTCTTACCGACGACAA
TCCAGACAAGCCGAAATATTATGTCCTCGACATGTTCCCCTACCCGAGCGGTACCGGCCTGCATGTCGGTCATCTCGAAG
GCTATACCGCAACCGATATCATAGCCCGCTACAAACGCAGTCGGGGCTTCAATGTCCTGCACCCTATGGGATGGGACGCT
TTCGGCCTTCCGGCAGAACAGTTTGCCATCAAAACCGGCACGCACCCTACAACGACCACACAGAAGAACGTAAGCAGTTT
CCGCCAGACCCTCCAGAACATGGGATTCTCCTACGACTGGTCCCGGGAAATAAACACGACCGACCCGAATTACTTCCGAT
GGACTCAGTGGATCTTTCTGCAGCTGCTCGACAAGGGACTTGCCTACATGAGTGAAGTCGATGTCAACTGGTGCGAAGAT
CTCAGAGTGGTCCTTGCCAATGAAGAGGTTGATGAAAAAATCGCGGCCGGCCACACCGTTGTGCGCAAACCGCTGCGGCA
GTGGGTCCTGAAAATCACAGCCTATGCAGAACGCCTGCTCGGCGACCTTGACGAGCTTGACTGGCCGGAAAATGTCAAGC
AGATGCAGCGCAACTGGATCGGGCGATCGGAAGGCGTCGAAATCGATTTTGAACTCCCCTGCCATCGCCAGAAACTTACC
GTGTATACGACCAGACCCGACACGCTTTTCGGCGCTTCCTACCTGGTTATTTCTCCGGAACATCCACTGGCCGAAAAACT
TGCTACAGCACCGCAGCTTGTCGAGCTCAAAAACTATATCCGCGAAGCAAAGCTGAAAACCGAGCTTGAACGCACCGGAC
TGCAGAAAGAAAAAACCGGAGTCTTCACCGGCTCCTATGCCATCAACCCCGCAAACGGTGAGGCCCTGCCGGTCTGGGTT
TCCGATTTTGTACTGACCTCCTACGGCACCGGCGCAATCATGTCGGTCCCGGCGCACGACAGCCGTGACTGGGAATTTGC
AAAAAAATTCGGGCTGCCGATCATCGAAGTCATTCAAAGTCCCCACGATGTTCAGGATGAGGTATTCGAAGGCAAAGAGA
GTATCTGTGTCAACTCGTCGAACAACGAGATAAGCCTGGACGGTCTCGACTTTACAAGCGCCTTCAAACGCATGGCCGAC
TGGATCGAATCGAGTGGAAAAGGCAGAAGAAAAATCAACTATAAACTCCGCGACTGGATTTTCAGTCGCCAGCGTTACTG
GGGAGAACCGATTCCCGTCAAACATTATGATGACGGTTCCCTGCGCCCTGAAACCGATCTTCCGCTCACCCTCCCGGAGG
TTGAAGCCTATCACCCATCAACCACGGGAGAATCCCCTCTGGCAAACATCGAGGACTGGCTCTTCGGGCAGGACGAACAC
GGTGCGTTCAGGAGAGAAACAAATACCATGCCGCAATGGGCTGGCAGCTGCTGGTACTATCTGCGCTTTATCGATCCCTC
AAACCAGCAGCAACTGGTCGACCCGGACAAAGAACGCTACTGGATGAATGTTGACCTCTATATCGGAGGAGCTGAACATG
CTGTCCTTCACCTTCTCTATGCGCGTTTCTGGCACAAAGTGCTCTACGATCTTGGCGTTGTATCGACAAAAGAGCCGTTC
CAGAAACTCTTCAATCAGGGAATGATTCTCGGCGAAGACAATGAAAAGATGTCGAAATCGCGAGGAAACGTTATCCCTGC
CGATCATGTCATGCAAAGCTATGGAGCCGACGCTGTGCGTCTCTACGAAATGTTCCTCGGCCCTCTGGAACAGGTCAAAC
CCTGGAACACCAACGGAATTGAAGGCATCAGCCGTTTTCTGTCGAGAGTTTGGCGGATTGTCTACCCTGAGCAGGAGGGT
CCAGCCATGCTGACCGATACGCCTCTCGACGACGCGTTGACACGCAGGATGCATAAAACCATCAAGAAAGTCACCGAGGA
TACCGAACATCTCAAGTTCAATACCGCCATTGCCGAAATGATGGTTTTTGTCAACGAACTGCACAAAGCCGGATGCAGAA
ACCGCAAGGCCCTGGAAACCCTGCTGCTTCTCCTTGCTCCCTATGCACCGCATATCTGCGAAGAACTCTGGCAGGCTGCC
GGCAACAACGAGCCGATCGCCAGGGCGCCGTTCCCTGTTTTTGATCCGGCTCTGGCTGAAGACAATGAGCTCACCATAGC
CGTTCAGGTCAACGGCAAGCTCAGAGGAACCTTCCTGGCTCCTGCAGGTTTATCGAAAGAGGCCATGATCGCCGGGGCCA
GAGAGATTGAAAGTGTGAAGAAATTCCTTGAGGGAATGACAATAATAAAAGAAATTGCCGTTCCCGGAAAACTGGTCAAC
TTCGCAGTCAAACCGCTCTGA

Upstream 100 bases:

>100_bases
CAAGGGGGAGACAATGACTGAACCTCTAATGATCAAAGCTGTTCCGGCATGACAGCACAGAAAGCCGCCAGGAAAAGCTC
AACACAATTTAAACGGTACC

Downstream 100 bases:

>100_bases
ACGATCCCCCAGATCTTCCGCAATCCCCCTTGAAAAAGGGGGATTTGCTCTGATTTTATCCCGACTCGCGCATTGTCAAA
AAAAATATTCCCCGACCAGA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 806; Mature: 806

Protein sequence:

>806_residues
MRYEFSTIEQKWQKYWADNQTFLTDDNPDKPKYYVLDMFPYPSGTGLHVGHLEGYTATDIIARYKRSRGFNVLHPMGWDA
FGLPAEQFAIKTGTHPTTTTQKNVSSFRQTLQNMGFSYDWSREINTTDPNYFRWTQWIFLQLLDKGLAYMSEVDVNWCED
LRVVLANEEVDEKIAAGHTVVRKPLRQWVLKITAYAERLLGDLDELDWPENVKQMQRNWIGRSEGVEIDFELPCHRQKLT
VYTTRPDTLFGASYLVISPEHPLAEKLATAPQLVELKNYIREAKLKTELERTGLQKEKTGVFTGSYAINPANGEALPVWV
SDFVLTSYGTGAIMSVPAHDSRDWEFAKKFGLPIIEVIQSPHDVQDEVFEGKESICVNSSNNEISLDGLDFTSAFKRMAD
WIESSGKGRRKINYKLRDWIFSRQRYWGEPIPVKHYDDGSLRPETDLPLTLPEVEAYHPSTTGESPLANIEDWLFGQDEH
GAFRRETNTMPQWAGSCWYYLRFIDPSNQQQLVDPDKERYWMNVDLYIGGAEHAVLHLLYARFWHKVLYDLGVVSTKEPF
QKLFNQGMILGEDNEKMSKSRGNVIPADHVMQSYGADAVRLYEMFLGPLEQVKPWNTNGIEGISRFLSRVWRIVYPEQEG
PAMLTDTPLDDALTRRMHKTIKKVTEDTEHLKFNTAIAEMMVFVNELHKAGCRNRKALETLLLLLAPYAPHICEELWQAA
GNNEPIARAPFPVFDPALAEDNELTIAVQVNGKLRGTFLAPAGLSKEAMIAGAREIESVKKFLEGMTIIKEIAVPGKLVN
FAVKPL

Sequences:

>Translated_806_residues
MRYEFSTIEQKWQKYWADNQTFLTDDNPDKPKYYVLDMFPYPSGTGLHVGHLEGYTATDIIARYKRSRGFNVLHPMGWDA
FGLPAEQFAIKTGTHPTTTTQKNVSSFRQTLQNMGFSYDWSREINTTDPNYFRWTQWIFLQLLDKGLAYMSEVDVNWCED
LRVVLANEEVDEKIAAGHTVVRKPLRQWVLKITAYAERLLGDLDELDWPENVKQMQRNWIGRSEGVEIDFELPCHRQKLT
VYTTRPDTLFGASYLVISPEHPLAEKLATAPQLVELKNYIREAKLKTELERTGLQKEKTGVFTGSYAINPANGEALPVWV
SDFVLTSYGTGAIMSVPAHDSRDWEFAKKFGLPIIEVIQSPHDVQDEVFEGKESICVNSSNNEISLDGLDFTSAFKRMAD
WIESSGKGRRKINYKLRDWIFSRQRYWGEPIPVKHYDDGSLRPETDLPLTLPEVEAYHPSTTGESPLANIEDWLFGQDEH
GAFRRETNTMPQWAGSCWYYLRFIDPSNQQQLVDPDKERYWMNVDLYIGGAEHAVLHLLYARFWHKVLYDLGVVSTKEPF
QKLFNQGMILGEDNEKMSKSRGNVIPADHVMQSYGADAVRLYEMFLGPLEQVKPWNTNGIEGISRFLSRVWRIVYPEQEG
PAMLTDTPLDDALTRRMHKTIKKVTEDTEHLKFNTAIAEMMVFVNELHKAGCRNRKALETLLLLLAPYAPHICEELWQAA
GNNEPIARAPFPVFDPALAEDNELTIAVQVNGKLRGTFLAPAGLSKEAMIAGAREIESVKKFLEGMTIIKEIAVPGKLVN
FAVKPL
>Mature_806_residues
MRYEFSTIEQKWQKYWADNQTFLTDDNPDKPKYYVLDMFPYPSGTGLHVGHLEGYTATDIIARYKRSRGFNVLHPMGWDA
FGLPAEQFAIKTGTHPTTTTQKNVSSFRQTLQNMGFSYDWSREINTTDPNYFRWTQWIFLQLLDKGLAYMSEVDVNWCED
LRVVLANEEVDEKIAAGHTVVRKPLRQWVLKITAYAERLLGDLDELDWPENVKQMQRNWIGRSEGVEIDFELPCHRQKLT
VYTTRPDTLFGASYLVISPEHPLAEKLATAPQLVELKNYIREAKLKTELERTGLQKEKTGVFTGSYAINPANGEALPVWV
SDFVLTSYGTGAIMSVPAHDSRDWEFAKKFGLPIIEVIQSPHDVQDEVFEGKESICVNSSNNEISLDGLDFTSAFKRMAD
WIESSGKGRRKINYKLRDWIFSRQRYWGEPIPVKHYDDGSLRPETDLPLTLPEVEAYHPSTTGESPLANIEDWLFGQDEH
GAFRRETNTMPQWAGSCWYYLRFIDPSNQQQLVDPDKERYWMNVDLYIGGAEHAVLHLLYARFWHKVLYDLGVVSTKEPF
QKLFNQGMILGEDNEKMSKSRGNVIPADHVMQSYGADAVRLYEMFLGPLEQVKPWNTNGIEGISRFLSRVWRIVYPEQEG
PAMLTDTPLDDALTRRMHKTIKKVTEDTEHLKFNTAIAEMMVFVNELHKAGCRNRKALETLLLLLAPYAPHICEELWQAA
GNNEPIARAPFPVFDPALAEDNELTIAVQVNGKLRGTFLAPAGLSKEAMIAGAREIESVKKFLEGMTIIKEIAVPGKLVN
FAVKPL

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=878, Percent_Identity=32.8018223234624, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI94721239, Length=679, Percent_Identity=22.2385861561119, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI94721241, Length=679, Percent_Identity=22.2385861561119, Blast_Score=99, Evalue=2e-20,
Organism=Escherichia coli, GI1786861, Length=868, Percent_Identity=42.3963133640553, Blast_Score=640, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71997517, Length=774, Percent_Identity=28.1653746770026, Blast_Score=280, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI71997510, Length=774, Percent_Identity=28.1653746770026, Blast_Score=280, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI212645227, Length=292, Percent_Identity=27.0547945205479, Blast_Score=116, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17510661, Length=377, Percent_Identity=23.342175066313, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323414, Length=766, Percent_Identity=35.2480417754569, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6321531, Length=498, Percent_Identity=21.8875502008032, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325217, Length=344, Percent_Identity=23.8372093023256, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI21355409, Length=882, Percent_Identity=30.2721088435374, Blast_Score=343, Evalue=3e-94,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_PROA2 (B4S5G4)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015208.1
- ProteinModelPortal:   B4S5G4
- SMR:   B4S5G4
- GeneID:   6459499
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_0505
- HOGENOM:   HBG693845
- OMA:   ERGNFPV
- ProtClustDB:   PRK00390
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 92201; Mature: 92201

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 579-579

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYEFSTIEQKWQKYWADNQTFLTDDNPDKPKYYVLDMFPYPSGTGLHVGHLEGYTATDI
CCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHH
IARYKRSRGFNVLHPMGWDAFGLPAEQFAIKTGTHPTTTTQKNVSSFRQTLQNMGFSYDW
HHHHHHCCCCCEECCCCCCCCCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEC
SREINTTDPNYFRWTQWIFLQLLDKGLAYMSEVDVNWCEDLRVVLANEEVDEKIAAGHTV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEECCCHHHHHHHHCHHH
VRKPLRQWVLKITAYAERLLGDLDELDWPENVKQMQRNWIGRSEGVEIDFELPCHRQKLT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEE
VYTTRPDTLFGASYLVISPEHPLAEKLATAPQLVELKNYIREAKLKTELERTGLQKEKTG
EEECCCCCCCCCEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
VFTGSYAINPANGEALPVWVSDFVLTSYGTGAIMSVPAHDSRDWEFAKKFGLPIIEVIQS
EEECCEEECCCCCCEEEEEHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHCCHHHHHHCC
PHDVQDEVFEGKESICVNSSNNEISLDGLDFTSAFKRMADWIESSGKGRRKINYKLRDWI
CCCHHHHHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHH
FSRQRYWGEPIPVKHYDDGSLRPETDLPLTLPEVEAYHPSTTGESPLANIEDWLFGQDEH
HHHHHHCCCCCCCEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
GAFRRETNTMPQWAGSCWYYLRFIDPSNQQQLVDPDKERYWMNVDLYIGGAEHAVLHLLY
CCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHH
ARFWHKVLYDLGVVSTKEPFQKLFNQGMILGEDNEKMSKSRGNVIPADHVMQSYGADAVR
HHHHHHHHHHHCCCCCHHHHHHHHHCCEEECCCCHHHHHHCCCCCCHHHHHHHHCCHHHH
LYEMFLGPLEQVKPWNTNGIEGISRFLSRVWRIVYPEQEGPAMLTDTPLDDALTRRMHKT
HHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHEECCCCCCCEEEECCCCHHHHHHHHHHH
IKKVTEDTEHLKFNTAIAEMMVFVNELHKAGCRNRKALETLLLLLAPYAPHICEELWQAA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
GNNEPIARAPFPVFDPALAEDNELTIAVQVNGKLRGTFLAPAGLSKEAMIAGAREIESVK
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHH
KFLEGMTIIKEIAVPGKLVNFAVKPL
HHHHHHHHHHHHCCCCHHHHHCCCCC
>Mature Secondary Structure
MRYEFSTIEQKWQKYWADNQTFLTDDNPDKPKYYVLDMFPYPSGTGLHVGHLEGYTATDI
CCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHH
IARYKRSRGFNVLHPMGWDAFGLPAEQFAIKTGTHPTTTTQKNVSSFRQTLQNMGFSYDW
HHHHHHCCCCCEECCCCCCCCCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEC
SREINTTDPNYFRWTQWIFLQLLDKGLAYMSEVDVNWCEDLRVVLANEEVDEKIAAGHTV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEECCCHHHHHHHHCHHH
VRKPLRQWVLKITAYAERLLGDLDELDWPENVKQMQRNWIGRSEGVEIDFELPCHRQKLT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEE
VYTTRPDTLFGASYLVISPEHPLAEKLATAPQLVELKNYIREAKLKTELERTGLQKEKTG
EEECCCCCCCCCEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
VFTGSYAINPANGEALPVWVSDFVLTSYGTGAIMSVPAHDSRDWEFAKKFGLPIIEVIQS
EEECCEEECCCCCCEEEEEHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHCCHHHHHHCC
PHDVQDEVFEGKESICVNSSNNEISLDGLDFTSAFKRMADWIESSGKGRRKINYKLRDWI
CCCHHHHHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEECHHHHHHH
FSRQRYWGEPIPVKHYDDGSLRPETDLPLTLPEVEAYHPSTTGESPLANIEDWLFGQDEH
HHHHHHCCCCCCCEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
GAFRRETNTMPQWAGSCWYYLRFIDPSNQQQLVDPDKERYWMNVDLYIGGAEHAVLHLLY
CCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHH
ARFWHKVLYDLGVVSTKEPFQKLFNQGMILGEDNEKMSKSRGNVIPADHVMQSYGADAVR
HHHHHHHHHHHCCCCCHHHHHHHHHCCEEECCCCHHHHHHCCCCCCHHHHHHHHCCHHHH
LYEMFLGPLEQVKPWNTNGIEGISRFLSRVWRIVYPEQEGPAMLTDTPLDDALTRRMHKT
HHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHEECCCCCCCEEEECCCCHHHHHHHHHHH
IKKVTEDTEHLKFNTAIAEMMVFVNELHKAGCRNRKALETLLLLLAPYAPHICEELWQAA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
GNNEPIARAPFPVFDPALAEDNELTIAVQVNGKLRGTFLAPAGLSKEAMIAGAREIESVK
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHH
KFLEGMTIIKEIAVPGKLVNFAVKPL
HHHHHHHHHHHHCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA