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The map label for this gene is aegA [H]

Identifier: 194333262

GI number: 194333262

Start: 452653

End: 454614

Strand: Reverse

Name: aegA [H]

Synonym: Paes_0418

Alternate gene names: 194333262

Gene position: 454614-452653 (Counterclockwise)

Preceding gene: 194333263

Following gene: 194333255

Centisome position: 18.09

GC content: 54.28

Gene sequence:

>1962_bases
ATGGCAACTGTTTCACTGACTCTGAACGGGAAAACCATTACAGCCGATGCTCATGTCTCAATTCTGGAGGCAGCCAAAAG
CAATGGCGTTATCATTCCAACGCTCTGTTTTCATCAGGAACTCAGTGCTCTGGGGTCTTGCTGGATGTGCATTGTCGAAC
TCAAGGGTAAAAACCGTTTTGTCCCGGCCTGCAGCACCAGGGTAACCAGCGGGATGGATATTGAAACCGACAACCCGGAA
CTGCATGCAATGCGCCGCAAAACCCTTGAACGGCTCATTGATCAGCATTGCGGCGATTGCCTGGGACCGTGCGAAACAGC
GTGCCCTGCCGGCTGCGACATTCCGGGCTATACGGCAGCTATCGCCAGAGGCGATGACCGCGAAGCAATCCGGATTATCA
AAGAAACCGTTCCTCTTGCTGCATCTCTTGGCAGAATATGCCCGGCTCCATGTGAAGATGCCTGCCGTCGTCACGGCGTC
GACGATCCAGTCTCGATCTGTGCCTTGAAACGCTTTGCCGCTGATAAAGACATCGCGTCAGCCGAACCGTTCGTTCCCGA
AAGAAAAAAAGCGTCAGGCAAAAAAGTCGCCGTCATCGGAGCCGGCCCTGCAGGCCTTACAGCCGCGTACTACCTGCTTC
GAAATGGTCACGATGTCACTGTTTTCGATGCAAACGACACACCGGGCGGCATGCTCTATTACGGCATTCCGCGATTCCGC
CTCCCTGCGACGATCCTTGAAGCCGACATTGCCCCCCTGCGGGCTATGGGTGGAGAAATCGTTCTCAATACCGTTCTGGG
GCGGGATATCTCCCTGAAAGAGCTGAAAAAAAATGGTTTTGATGCAACCTATATCGCCGTAGGAGCTGCCAAAGCATCGA
CAATGGGTATTGCCGGAGAAGAACTTCCCGGAGTCGTTTCCGGAATCGGATTTCTCAGGGATATAGCCTCGGGAAAAGCG
GTACGGACAGGAAAAAGGGTTGTTGTTGTCGGAGGCGGGAACACGGCTGTCGATGCAGCAAGAACAGCCCTCCGAACCGG
AGCTGAATCGGTCACCATTATCTATCGAAGAACTCGTGAAGAGATGCCTGCCAACCAGGCTGAAATCGAGGAAGCCCTTG
CCGAAGGCATCGCTCTGCAATGCCTTGCCGCTCCATCGGCCATAACCTCCTGTGAAAGCGGCCTGGAAATGACCGCACTG
AAGATGCTGCAGGGCGAACCTGATGAAAGCGGACGCCGACGCCCTGTTGCCTTAAACGGCTCGGAGTTCTCTCTCTTTGC
CGACACGATCATTGCCGCTATCGGCCAGTATGTCGATCCTGAAATCCAGACCGCAACAGGGTTGCAACTATCGCAAAAAG
GCACCTTCAAGACCGACAGTGCCACATGCATGACAGAACAGGAGGGCGTCTTTGCGGGAGGAGACTGCGTTATCGGTGCC
GATATCGCCATCAATGCCATCGAGCAGGGAAAACGTGCAGCAGACGCTATCGACAGCTTTCTCAACGCCGTCACTTCCCG
GAACGAGAAAAAACCCTTCAACTCAACCTACGGACCAAGGGATAACGCCCCTGAAACATTTCTTATGCGGGCAAAACCCA
TCAAACGCGCGTCGATGCAGGAACTTCAAGGACCTGAAAGAACATCGACATTCCGCGAGGTCGCCCTTGGCCTTGACGAA
GAACAGGCGCGTCTTGAGGCGCAGCGATGCCTGCGATGCAGCTGTACGACAAAAAACAACTGCTCGCTCCGCCGCCTGGC
TTCACGCTATGAGGTATCGTCAGCGCAATGCGATGTCGAAACGCATGACAACGCCTATATTACCCGCGGAAATGGAATAC
GGTTTGAACGGGAAAAATGTGTTGATTGCGGCATCTGCGTGCGAACCATCGAAGAATACGGTCCTGAAGAGCTCAGGAGC
ACAATCCTCATAGAACGCTGCCCTACCGGAGCACTGAGCTAA

Upstream 100 bases:

>100_bases
CTGGAAAAAGTTGCAAGAAGCATAGAGAGCAGATTTTCCTCCTGAACCCGCTCATGCCTGGCAGAAACTCTACATTTTTC
AAGAAAATCGCTATTCAATC

Downstream 100 bases:

>100_bases
ACGGAAGCATTGGCCTGTTGACCTCTTGAACGGTTAACAACGGCGTCAACAGGCCAAACAGTCCTACAGCTTTGAAAAAT
CAAACTCCCGGCTTTCAAAC

Product: putative NADPH-dependent glutamate synthase small subunit

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MATVSLTLNGKTITADAHVSILEAAKSNGVIIPTLCFHQELSALGSCWMCIVELKGKNRFVPACSTRVTSGMDIETDNPE
LHAMRRKTLERLIDQHCGDCLGPCETACPAGCDIPGYTAAIARGDDREAIRIIKETVPLAASLGRICPAPCEDACRRHGV
DDPVSICALKRFAADKDIASAEPFVPERKKASGKKVAVIGAGPAGLTAAYYLLRNGHDVTVFDANDTPGGMLYYGIPRFR
LPATILEADIAPLRAMGGEIVLNTVLGRDISLKELKKNGFDATYIAVGAAKASTMGIAGEELPGVVSGIGFLRDIASGKA
VRTGKRVVVVGGGNTAVDAARTALRTGAESVTIIYRRTREEMPANQAEIEEALAEGIALQCLAAPSAITSCESGLEMTAL
KMLQGEPDESGRRRPVALNGSEFSLFADTIIAAIGQYVDPEIQTATGLQLSQKGTFKTDSATCMTEQEGVFAGGDCVIGA
DIAINAIEQGKRAADAIDSFLNAVTSRNEKKPFNSTYGPRDNAPETFLMRAKPIKRASMQELQGPERTSTFREVALGLDE
EQARLEAQRCLRCSCTTKNNCSLRRLASRYEVSSAQCDVETHDNAYITRGNGIRFEREKCVDCGICVRTIEEYGPEELRS
TILIERCPTGALS

Sequences:

>Translated_653_residues
MATVSLTLNGKTITADAHVSILEAAKSNGVIIPTLCFHQELSALGSCWMCIVELKGKNRFVPACSTRVTSGMDIETDNPE
LHAMRRKTLERLIDQHCGDCLGPCETACPAGCDIPGYTAAIARGDDREAIRIIKETVPLAASLGRICPAPCEDACRRHGV
DDPVSICALKRFAADKDIASAEPFVPERKKASGKKVAVIGAGPAGLTAAYYLLRNGHDVTVFDANDTPGGMLYYGIPRFR
LPATILEADIAPLRAMGGEIVLNTVLGRDISLKELKKNGFDATYIAVGAAKASTMGIAGEELPGVVSGIGFLRDIASGKA
VRTGKRVVVVGGGNTAVDAARTALRTGAESVTIIYRRTREEMPANQAEIEEALAEGIALQCLAAPSAITSCESGLEMTAL
KMLQGEPDESGRRRPVALNGSEFSLFADTIIAAIGQYVDPEIQTATGLQLSQKGTFKTDSATCMTEQEGVFAGGDCVIGA
DIAINAIEQGKRAADAIDSFLNAVTSRNEKKPFNSTYGPRDNAPETFLMRAKPIKRASMQELQGPERTSTFREVALGLDE
EQARLEAQRCLRCSCTTKNNCSLRRLASRYEVSSAQCDVETHDNAYITRGNGIRFEREKCVDCGICVRTIEEYGPEELRS
TILIERCPTGALS
>Mature_652_residues
ATVSLTLNGKTITADAHVSILEAAKSNGVIIPTLCFHQELSALGSCWMCIVELKGKNRFVPACSTRVTSGMDIETDNPEL
HAMRRKTLERLIDQHCGDCLGPCETACPAGCDIPGYTAAIARGDDREAIRIIKETVPLAASLGRICPAPCEDACRRHGVD
DPVSICALKRFAADKDIASAEPFVPERKKASGKKVAVIGAGPAGLTAAYYLLRNGHDVTVFDANDTPGGMLYYGIPRFRL
PATILEADIAPLRAMGGEIVLNTVLGRDISLKELKKNGFDATYIAVGAAKASTMGIAGEELPGVVSGIGFLRDIASGKAV
RTGKRVVVVGGGNTAVDAARTALRTGAESVTIIYRRTREEMPANQAEIEEALAEGIALQCLAAPSAITSCESGLEMTALK
MLQGEPDESGRRRPVALNGSEFSLFADTIIAAIGQYVDPEIQTATGLQLSQKGTFKTDSATCMTEQEGVFAGGDCVIGAD
IAINAIEQGKRAADAIDSFLNAVTSRNEKKPFNSTYGPRDNAPETFLMRAKPIKRASMQELQGPERTSTFREVALGLDEE
QARLEAQRCLRCSCTTKNNCSLRRLASRYEVSSAQCDVETHDNAYITRGNGIRFEREKCVDCGICVRTIEEYGPEELRST
ILIERCPTGALS

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=441, Percent_Identity=28.3446712018141, Blast_Score=163, Evalue=6e-40,
Organism=Escherichia coli, GI1788811, Length=422, Percent_Identity=35.781990521327, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1789243, Length=418, Percent_Identity=37.799043062201, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1789606, Length=431, Percent_Identity=35.7308584686775, Blast_Score=220, Evalue=3e-58,
Organism=Escherichia coli, GI87082180, Length=428, Percent_Identity=35.981308411215, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1788468, Length=407, Percent_Identity=32.6781326781327, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17570289, Length=451, Percent_Identity=27.0509977827051, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71984108, Length=440, Percent_Identity=29.0909090909091, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17543860, Length=163, Percent_Identity=33.7423312883436, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17565758, Length=97, Percent_Identity=31.9587628865979, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32566231, Length=97, Percent_Identity=31.9587628865979, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193209088, Length=124, Percent_Identity=29.8387096774194, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=441, Percent_Identity=31.2925170068027, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI28574881, Length=445, Percent_Identity=31.2359550561798, Blast_Score=179, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24665539, Length=445, Percent_Identity=31.2359550561798, Blast_Score=179, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24665547, Length=445, Percent_Identity=31.2359550561798, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24665543, Length=445, Percent_Identity=31.2359550561798, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24640763, Length=431, Percent_Identity=27.3781902552204, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI18858217, Length=431, Percent_Identity=27.3781902552204, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 69868; Mature: 69737

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVSLTLNGKTITADAHVSILEAAKSNGVIIPTLCFHQELSALGSCWMCIVELKGKNRF
CEEEEEEECCEEEECCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHEEEEEEECCCCCC
VPACSTRVTSGMDIETDNPELHAMRRKTLERLIDQHCGDCLGPCETACPAGCDIPGYTAA
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCEEE
IARGDDREAIRIIKETVPLAASLGRICPAPCEDACRRHGVDDPVSICALKRFAADKDIAS
EECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
AEPFVPERKKASGKKVAVIGAGPAGLTAAYYLLRNGHDVTVFDANDTPGGMLYYGIPRFR
CCCCCCCHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCC
LPATILEADIAPLRAMGGEIVLNTVLGRDISLKELKKNGFDATYIAVGAAKASTMGIAGE
CCHHHHHHHHHHHHHCCCCEEEEHHHCCCCCHHHHHHCCCCEEEEEEECCHHHHCCCCCH
ELPGVVSGIGFLRDIASGKAVRTGKRVVVVGGGNTAVDAARTALRTGAESVTIIYRRTRE
HCCHHHHHHHHHHHHHCCCEEECCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEHHHH
EMPANQAEIEEALAEGIALQCLAAPSAITSCESGLEMTALKMLQGEPDESGRRRPVALNG
HCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEECC
SEFSLFADTIIAAIGQYVDPEIQTATGLQLSQKGTFKTDSATCMTEQEGVFAGGDCVIGA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCEEEECCCCEEECCCEEEEC
DIAINAIEQGKRAADAIDSFLNAVTSRNEKKPFNSTYGPRDNAPETFLMRAKPIKRASMQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHH
ELQGPERTSTFREVALGLDEEQARLEAQRCLRCSCTTKNNCSLRRLASRYEVSSAQCDVE
HHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCEECEE
THDNAYITRGNGIRFEREKCVDCGICVRTIEEYGPEELRSTILIERCPTGALS
ECCCEEEECCCCCEECHHHHCHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCC
>Mature Secondary Structure 
ATVSLTLNGKTITADAHVSILEAAKSNGVIIPTLCFHQELSALGSCWMCIVELKGKNRF
EEEEEEECCEEEECCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHEEEEEEECCCCCC
VPACSTRVTSGMDIETDNPELHAMRRKTLERLIDQHCGDCLGPCETACPAGCDIPGYTAA
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCEEE
IARGDDREAIRIIKETVPLAASLGRICPAPCEDACRRHGVDDPVSICALKRFAADKDIAS
EECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
AEPFVPERKKASGKKVAVIGAGPAGLTAAYYLLRNGHDVTVFDANDTPGGMLYYGIPRFR
CCCCCCCHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCC
LPATILEADIAPLRAMGGEIVLNTVLGRDISLKELKKNGFDATYIAVGAAKASTMGIAGE
CCHHHHHHHHHHHHHCCCCEEEEHHHCCCCCHHHHHHCCCCEEEEEEECCHHHHCCCCCH
ELPGVVSGIGFLRDIASGKAVRTGKRVVVVGGGNTAVDAARTALRTGAESVTIIYRRTRE
HCCHHHHHHHHHHHHHCCCEEECCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEHHHH
EMPANQAEIEEALAEGIALQCLAAPSAITSCESGLEMTALKMLQGEPDESGRRRPVALNG
HCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEECC
SEFSLFADTIIAAIGQYVDPEIQTATGLQLSQKGTFKTDSATCMTEQEGVFAGGDCVIGA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCEEEECCCCEEECCCEEEEC
DIAINAIEQGKRAADAIDSFLNAVTSRNEKKPFNSTYGPRDNAPETFLMRAKPIKRASMQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHH
ELQGPERTSTFREVALGLDEEQARLEAQRCLRCSCTTKNNCSLRRLASRYEVSSAQCDVE
HHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCEECEE
THDNAYITRGNGIRFEREKCVDCGICVRTIEEYGPEELRSTILIERCPTGALS
ECCCEEEECCCCCEECHHHHCHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]