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The map label for this gene is ispH

Identifier: 194333263

GI number: 194333263

Start: 454670

End: 455647

Strand: Reverse

Name: ispH

Synonym: Paes_0419

Alternate gene names: 194333263

Gene position: 455647-454670 (Counterclockwise)

Preceding gene: 194333264

Following gene: 194333262

Centisome position: 18.13

GC content: 50.41

Gene sequence:

>978_bases
GTGAAAGTTAATCTTGACAGAACATCTTCAGGTTTCTGCATAGGCGTGCAGGGCACCATCCACCTCGCTGAAGAAAAACT
CAGGGAACATGACGGCAAACTCTACTGCCTCGGCGACATTGTACACAATGAAGTCGAAGTCAAACGACTGGAGGAACTGG
GGCTCATCACGATAGATCTCAATGAATACAGCGCGCTAACCAACGCACCTGTTCTGATTCGCGCCCATGGAGAACCCCCT
TCGACCTATACCACAGCAAAAAGCAACCAGCTCGACGTTACCGATTCGACCTGCCCGGTCGTCGCAAAACTGCAACGCTC
AGCAAGAATGCTGCATAATCGGGGATATCAGATTATTATCTACGGCAAAAAAAACCATCCGGAAGTCATCGGCATCAATG
GTCAATGCGATAACCAGGCCATTATCATCAAACACCCTGACCTTTCAGCCCCTGAAGAAACCGCCCCGCTTGATTTTACC
CGGCGTACCGCGCTCATATCCCAGACAACCATGGATGTGCCGGGATTTGCTATGCTGAAAGAAAATCTTGAACGCCTGTT
TTCGGCTTCTGCTGGACCAGGAGAGCGTCATGCGGCATCGCACTGGCAGGATATCAGGGATATCGACCTTACAGCAGAAA
TAACCGGTGTACGTCCCCTGCCGAAATTTCTCTACAAAGATACGATCTGCCGTCAGGTATCGAGCAGAAACAGTAAACTG
CATGACTTCGCCGCGCAGAACGACGTTGTCATCTTCGTTGCCGGAAAGAAAAGCTCAAATGGACAGGTACTCTTCAACAT
CTGTAAAGCCTCAAACCCGCGCACCTATTTTGCCGAGGATGAAAAAGACATACAAGATGAATGGCTGCTGGAAAACCGCC
AGCCCGTCCAAAGCGCAGGGGTCTGTGGAGCGACATCGACGCCGATGTGGCTCCTGGAAAAAGTTGCAAGAAGCATAGAG
AGCAGATTTTCCTCCTGA

Upstream 100 bases:

>100_bases
TTCCAACTTTCTGCCATTTTGCTATATTCTCTTCATCCACAACGTTCATTTATGGCAGCAGAGCTCATCTGCCTGTCGGA
ATTCTTAGTTACTCACTATC

Downstream 100 bases:

>100_bases
ACCCGCTCATGCCTGGCAGAAACTCTACATTTTTCAAGAAAATCGCTATTCAATCATGGCAACTGTTTCACTGACTCTGA
ACGGGAAAACCATTACAGCC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKVNLDRTSSGFCIGVQGTIHLAEEKLREHDGKLYCLGDIVHNEVEVKRLEELGLITIDLNEYSALTNAPVLIRAHGEPP
STYTTAKSNQLDVTDSTCPVVAKLQRSARMLHNRGYQIIIYGKKNHPEVIGINGQCDNQAIIIKHPDLSAPEETAPLDFT
RRTALISQTTMDVPGFAMLKENLERLFSASAGPGERHAASHWQDIRDIDLTAEITGVRPLPKFLYKDTICRQVSSRNSKL
HDFAAQNDVVIFVAGKKSSNGQVLFNICKASNPRTYFAEDEKDIQDEWLLENRQPVQSAGVCGATSTPMWLLEKVARSIE
SRFSS

Sequences:

>Translated_325_residues
MKVNLDRTSSGFCIGVQGTIHLAEEKLREHDGKLYCLGDIVHNEVEVKRLEELGLITIDLNEYSALTNAPVLIRAHGEPP
STYTTAKSNQLDVTDSTCPVVAKLQRSARMLHNRGYQIIIYGKKNHPEVIGINGQCDNQAIIIKHPDLSAPEETAPLDFT
RRTALISQTTMDVPGFAMLKENLERLFSASAGPGERHAASHWQDIRDIDLTAEITGVRPLPKFLYKDTICRQVSSRNSKL
HDFAAQNDVVIFVAGKKSSNGQVLFNICKASNPRTYFAEDEKDIQDEWLLENRQPVQSAGVCGATSTPMWLLEKVARSIE
SRFSS
>Mature_325_residues
MKVNLDRTSSGFCIGVQGTIHLAEEKLREHDGKLYCLGDIVHNEVEVKRLEELGLITIDLNEYSALTNAPVLIRAHGEPP
STYTTAKSNQLDVTDSTCPVVAKLQRSARMLHNRGYQIIIYGKKNHPEVIGINGQCDNQAIIIKHPDLSAPEETAPLDFT
RRTALISQTTMDVPGFAMLKENLERLFSASAGPGERHAASHWQDIRDIDLTAEITGVRPLPKFLYKDTICRQVSSRNSKL
HDFAAQNDVVIFVAGKKSSNGQVLFNICKASNPRTYFAEDEKDIQDEWLLENRQPVQSAGVCGATSTPMWLLEKVARSIE
SRFSS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=318, Percent_Identity=28.3018867924528, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_PROA2 (B4S4X8)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002015123.1
- GeneID:   6458403
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_0419
- HOGENOM:   HBG335228
- OMA:   WGAPVYV
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 36158; Mature: 36158

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNLDRTSSGFCIGVQGTIHLAEEKLREHDGKLYCLGDIVHNEVEVKRLEELGLITIDL
CEEECCCCCCCEEEEECCEEHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHCCEEEEEC
NEYSALTNAPVLIRAHGEPPSTYTTAKSNQLDVTDSTCPVVAKLQRSARMLHNRGYQIII
CHHHHHCCCCEEEEECCCCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEE
YGKKNHPEVIGINGQCDNQAIIIKHPDLSAPEETAPLDFTRRTALISQTTMDVPGFAMLK
EECCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
ENLERLFSASAGPGERHAASHWQDIRDIDLTAEITGVRPLPKFLYKDTICRQVSSRNSKL
HHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHH
HDFAAQNDVVIFVAGKKSSNGQVLFNICKASNPRTYFAEDEKDIQDEWLLENRQPVQSAG
HHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCEEECCCCCCCCHHHHHCCCCCHHHCC
VCGATSTPMWLLEKVARSIESRFSS
CCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVNLDRTSSGFCIGVQGTIHLAEEKLREHDGKLYCLGDIVHNEVEVKRLEELGLITIDL
CEEECCCCCCCEEEEECCEEHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHCCEEEEEC
NEYSALTNAPVLIRAHGEPPSTYTTAKSNQLDVTDSTCPVVAKLQRSARMLHNRGYQIII
CHHHHHCCCCEEEEECCCCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEE
YGKKNHPEVIGINGQCDNQAIIIKHPDLSAPEETAPLDFTRRTALISQTTMDVPGFAMLK
EECCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
ENLERLFSASAGPGERHAASHWQDIRDIDLTAEITGVRPLPKFLYKDTICRQVSSRNSKL
HHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHH
HDFAAQNDVVIFVAGKKSSNGQVLFNICKASNPRTYFAEDEKDIQDEWLLENRQPVQSAG
HHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCEEECCCCCCCCHHHHHCCCCCHHHCC
VCGATSTPMWLLEKVARSIESRFSS
CCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA