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The map label for this gene is ptsI [H]

Identifier: 194333181

GI number: 194333181

Start: 366477

End: 368252

Strand: Reverse

Name: ptsI [H]

Synonym: Paes_0337

Alternate gene names: 194333181

Gene position: 368252-366477 (Counterclockwise)

Preceding gene: 194333182

Following gene: 194333178

Centisome position: 14.65

GC content: 48.76

Gene sequence:

>1776_bases
ATGGAACCCATTCTCCCACAGAGCGACACAGGCACAAAGAAGAACAAAGCGGTATTTCACGGCATCGGTGCGTCGAAAGG
TATTGCGATCGCACCTGCGTTCCTGTTCATTAAAAATATTATTGAACACTCGTCGGCCCCTCTCTCCGCTGAAAAAGCAG
AGATTGAGGTAGACAAATTTCTTGCTGCCCTGCAGCGTTCTGAAAAAGAATTGAGAAAAATCGAGAAGGTCACCACCAAG
AAACTTGGAAAAGTCTACTCCGATCTTTTTCAGGCCCAGATCATGCTTCTCAACGACAGCGTGCTCATCGAGTGCGTCAC
AAAACGCATCCGCCAGGAACTCAAAAGCGCGCCGCTTGTTATCGAAGAGGAGTTCGAGAAATATCTTGAACACTTCAACA
ACTCGACAGAACAGATGTTTCAGGAGCGGGCCCAGGACCTGATCGATATCAAAAACCGCATCATCCGCAACCTGCACAAT
CAGAAACTGCAATCTAAAATCCCTGAGGGAATGATTGTCGTGTCCACGTGTCTCTCGCCGGCTGACATCATTCTCTTCAC
CAGAGCAAACGTCAAAGCCTTTATCACCGAGACCGGGGGCATCACATCACATATTTCGCTTATCTGCCGTTCGCTCAACA
TACCAATCATTGTAGGGCTGAGTAATTTCACCCAGAAAGTCAAAACAAACGATCTTCTTATCGTTGACGGAAGCAGCGGC
GAAGCGCTGATTAACCCCGACAGCGAAACCATACGCCAATACCAGAACCGCTCAGAAGAAAAAAGCCGTCTGGAAGCGCA
GACATGCAGGCTTGCCGACGCCCCGGCACAAACAAAATGCGGCCAGCCCCTTCACTTTTGTTCCAATATCGATTTCAAGG
AAGAAATTCCCTCTATCCGTTCTGTCGGTGCGCGGGGTGTAGGACTATTCAGAAGCGAAAACCTCTTTATAGACAACACC
AAGCCACCGCAGGAAGAAGACCAGACGGCCTATTACAAGGAAATGGCTGAAGAACTTTTTCCTGACCCCCTCGTCATCAG
ACTCTTCGATATTGGTGGAGACAAACTGATCTATTCAACGATCAGGGAACCAAACCCTAATCTGGGCTGGCGGGGAATCA
GAATTCTCATCGATGTTCCCGAAATTCTCGACAGTCAGCTCCTTGCCATTCTCAAAGCCAATGACAAAGGAAATATTCAG
ATCCTGCTTCCCATGATCTCTTCAATAGAGGAAATCATGCAGATCCAATCCTCCCTCAAACGCCATGTCGACAACCTCAA
TACCATCGGATTGAGCTGCTGTGAGCCTGACATCGGAGCCATGATCGAGATTCCCGCAGCAGTGCTCATTATCGATGAAA
TCACCAGAATGGTCGACTTTATCAGCATCGGAACCAATGATCTGACGCAATACACCCTGGCGGTAGACCGCAACAATGAA
ATCGTGCAGGACCTTTTCGATAAGTTCCATCCCGCAATCATCCGTCAGCTCCATACGATCATCACGACCGCAAACAAAAA
CGGCTGCAGAGTCTCACTCTGTGGAGATATGGGCTCCGACCCCCTGGCGCTGCCCTTCCTCGTCGGATGCGGGCTGAGAG
TGTTCAGCGTCGTAAGCGCCGACATTCCCTTACTGAAAACACTTGGAAGACATCTGGATGTCAAGGAAAGTGAAGCACTG
GCCAGAGAATGTCTTTCCTTTGATTCATCGGAAAAAATAAAAGCGCACCTGAAAGAGTTTCAGATGCGCCATGTTCCGCA
GAATGTACTGATGTAA

Upstream 100 bases:

>100_bases
CACCAGACCGCTTTATTGCTCCGGATGATGCCCCATTTTAGGTATCAGCAAGCAATAACTACAGTAATGAGAGCGTCTTA
ACATTTGTAAGCAGATTCCA

Downstream 100 bases:

>100_bases
ACCTATACTGCAATAAACACATCCCCCCTCCAGAACGAGGGATGATGCGTTTATTGACCTCCGACGCGCTTATAGTCTTC
TCATATCTTCAATCGTGGCC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MEPILPQSDTGTKKNKAVFHGIGASKGIAIAPAFLFIKNIIEHSSAPLSAEKAEIEVDKFLAALQRSEKELRKIEKVTTK
KLGKVYSDLFQAQIMLLNDSVLIECVTKRIRQELKSAPLVIEEEFEKYLEHFNNSTEQMFQERAQDLIDIKNRIIRNLHN
QKLQSKIPEGMIVVSTCLSPADIILFTRANVKAFITETGGITSHISLICRSLNIPIIVGLSNFTQKVKTNDLLIVDGSSG
EALINPDSETIRQYQNRSEEKSRLEAQTCRLADAPAQTKCGQPLHFCSNIDFKEEIPSIRSVGARGVGLFRSENLFIDNT
KPPQEEDQTAYYKEMAEELFPDPLVIRLFDIGGDKLIYSTIREPNPNLGWRGIRILIDVPEILDSQLLAILKANDKGNIQ
ILLPMISSIEEIMQIQSSLKRHVDNLNTIGLSCCEPDIGAMIEIPAAVLIIDEITRMVDFISIGTNDLTQYTLAVDRNNE
IVQDLFDKFHPAIIRQLHTIITTANKNGCRVSLCGDMGSDPLALPFLVGCGLRVFSVVSADIPLLKTLGRHLDVKESEAL
ARECLSFDSSEKIKAHLKEFQMRHVPQNVLM

Sequences:

>Translated_591_residues
MEPILPQSDTGTKKNKAVFHGIGASKGIAIAPAFLFIKNIIEHSSAPLSAEKAEIEVDKFLAALQRSEKELRKIEKVTTK
KLGKVYSDLFQAQIMLLNDSVLIECVTKRIRQELKSAPLVIEEEFEKYLEHFNNSTEQMFQERAQDLIDIKNRIIRNLHN
QKLQSKIPEGMIVVSTCLSPADIILFTRANVKAFITETGGITSHISLICRSLNIPIIVGLSNFTQKVKTNDLLIVDGSSG
EALINPDSETIRQYQNRSEEKSRLEAQTCRLADAPAQTKCGQPLHFCSNIDFKEEIPSIRSVGARGVGLFRSENLFIDNT
KPPQEEDQTAYYKEMAEELFPDPLVIRLFDIGGDKLIYSTIREPNPNLGWRGIRILIDVPEILDSQLLAILKANDKGNIQ
ILLPMISSIEEIMQIQSSLKRHVDNLNTIGLSCCEPDIGAMIEIPAAVLIIDEITRMVDFISIGTNDLTQYTLAVDRNNE
IVQDLFDKFHPAIIRQLHTIITTANKNGCRVSLCGDMGSDPLALPFLVGCGLRVFSVVSADIPLLKTLGRHLDVKESEAL
ARECLSFDSSEKIKAHLKEFQMRHVPQNVLM
>Mature_591_residues
MEPILPQSDTGTKKNKAVFHGIGASKGIAIAPAFLFIKNIIEHSSAPLSAEKAEIEVDKFLAALQRSEKELRKIEKVTTK
KLGKVYSDLFQAQIMLLNDSVLIECVTKRIRQELKSAPLVIEEEFEKYLEHFNNSTEQMFQERAQDLIDIKNRIIRNLHN
QKLQSKIPEGMIVVSTCLSPADIILFTRANVKAFITETGGITSHISLICRSLNIPIIVGLSNFTQKVKTNDLLIVDGSSG
EALINPDSETIRQYQNRSEEKSRLEAQTCRLADAPAQTKCGQPLHFCSNIDFKEEIPSIRSVGARGVGLFRSENLFIDNT
KPPQEEDQTAYYKEMAEELFPDPLVIRLFDIGGDKLIYSTIREPNPNLGWRGIRILIDVPEILDSQLLAILKANDKGNIQ
ILLPMISSIEEIMQIQSSLKRHVDNLNTIGLSCCEPDIGAMIEIPAAVLIIDEITRMVDFISIGTNDLTQYTLAVDRNNE
IVQDLFDKFHPAIIRQLHTIITTANKNGCRVSLCGDMGSDPLALPFLVGCGLRVFSVVSADIPLLKTLGRHLDVKESEAL
ARECLSFDSSEKIKAHLKEFQMRHVPQNVLM

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=568, Percent_Identity=34.330985915493, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI1789193, Length=604, Percent_Identity=31.6225165562914, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI48994992, Length=502, Percent_Identity=30.2788844621514, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1788726, Length=498, Percent_Identity=30.9236947791165, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI1787994, Length=410, Percent_Identity=24.390243902439, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 66080; Mature: 66080

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPILPQSDTGTKKNKAVFHGIGASKGIAIAPAFLFIKNIIEHSSAPLSAEKAEIEVDKF
CCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LAALQRSEKELRKIEKVTTKKLGKVYSDLFQAQIMLLNDSVLIECVTKRIRQELKSAPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCE
IEEEFEKYLEHFNNSTEQMFQERAQDLIDIKNRIIRNLHNQKLQSKIPEGMIVVSTCLSP
EHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCC
ADIILFTRANVKAFITETGGITSHISLICRSLNIPIIVGLSNFTQKVKTNDLLIVDGSSG
CCEEEEEECCCEEEEEECCCHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCEEEEECCCC
EALINPDSETIRQYQNRSEEKSRLEAQTCRLADAPAQTKCGQPLHFCSNIDFKEEIPSIR
CEEECCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHCCHHH
SVGARGVGLFRSENLFIDNTKPPQEEDQTAYYKEMAEELFPDPLVIRLFDIGGDKLIYST
HCCCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHH
IREPNPNLGWRGIRILIDVPEILDSQLLAILKANDKGNIQILLPMISSIEEIMQIQSSLK
HCCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
RHVDNLNTIGLSCCEPDIGAMIEIPAAVLIIDEITRMVDFISIGTNDLTQYTLAVDRNNE
HHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCH
IVQDLFDKFHPAIIRQLHTIITTANKNGCRVSLCGDMGSDPLALPFLVGCGLRVFSVVSA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHC
DIPLLKTLGRHLDVKESEALARECLSFDSSEKIKAHLKEFQMRHVPQNVLM
CCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure
MEPILPQSDTGTKKNKAVFHGIGASKGIAIAPAFLFIKNIIEHSSAPLSAEKAEIEVDKF
CCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LAALQRSEKELRKIEKVTTKKLGKVYSDLFQAQIMLLNDSVLIECVTKRIRQELKSAPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCE
IEEEFEKYLEHFNNSTEQMFQERAQDLIDIKNRIIRNLHNQKLQSKIPEGMIVVSTCLSP
EHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCC
ADIILFTRANVKAFITETGGITSHISLICRSLNIPIIVGLSNFTQKVKTNDLLIVDGSSG
CCEEEEEECCCEEEEEECCCHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCEEEEECCCC
EALINPDSETIRQYQNRSEEKSRLEAQTCRLADAPAQTKCGQPLHFCSNIDFKEEIPSIR
CEEECCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHCCHHH
SVGARGVGLFRSENLFIDNTKPPQEEDQTAYYKEMAEELFPDPLVIRLFDIGGDKLIYST
HCCCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHH
IREPNPNLGWRGIRILIDVPEILDSQLLAILKANDKGNIQILLPMISSIEEIMQIQSSLK
HCCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
RHVDNLNTIGLSCCEPDIGAMIEIPAAVLIIDEITRMVDFISIGTNDLTQYTLAVDRNNE
HHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCH
IVQDLFDKFHPAIIRQLHTIITTANKNGCRVSLCGDMGSDPLALPFLVGCGLRVFSVVSA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHC
DIPLLKTLGRHLDVKESEALARECLSFDSSEKIKAHLKEFQMRHVPQNVLM
CCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]