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The map label for this gene is rffG [H]

Identifier: 193213102

GI number: 193213102

Start: 1593983

End: 1594987

Strand: Direct

Name: rffG [H]

Synonym: Cpar_1457

Alternate gene names: 193213102

Gene position: 1593983-1594987 (Clockwise)

Preceding gene: 193213101

Following gene: 193213103

Centisome position: 69.63

GC content: 50.55

Gene sequence:

>1005_bases
ATGCAGCACCAGAACGATGAAAGCATGACAATCAAGCGATTGTTTCTGACTGGAGGGACCGGCTTCTTCGGAAAATCTCT
CCTTCGATACTGGATACAACAATCAACGCAGGGCAAATCTGTTCCGGCAGTAACCCTCATGAGCCGTTCTCCGGATGCCT
TTCTTGCATCCTATCCTGAGTTTGTTGGGCTGCCTTGGCTTACGCTTGTCAAGGGAGACGTTACAATGCCGAAAACTTTC
CCAACCGAGCTGAATTGTTCTCACATCCTGCATGCCGCCACGGACTCTACCAACGGCCCGATGTTGACTCCGCTGGAACG
CTTTAACCAGATTGTTGATGGTACGCGCAATGTGCTTGATTTTGCACATCGGGTCGAAGCAACACGTTTTCTGCTGACAA
GTTCCGGTGGCGTTTACGGCCCACAACCAGAAAAAATGGAGCGTATTCCCGAAGACTATAACGGTATGCCAGACCCGCTT
CAGCCAAGCCATGCGTATAGCGTTGCCAAGCGCACAGCCGAACACTTGTGTGCGCTGTATGGCGACGCATTTGGTATAGA
AATATTGGTGGCGCGTTGCTTTGCCTTCGTCGGCCCGGACTTGCCGCTTGATGCGCATTTTGCAATCGGGAACTTCATCG
CAGATGCTGTTGCCGGTCGTCAAATCACAATCAAGGGAAATGGGTTGCCGATTCGTTCTTATCTCGATCAAAGTGATCTT
GCAACATGGCTATTGACGATACTTGAACATGGTCAATCCGGTCGGCCCTACAATGTCGGATCCGATCAAGCGATCACTCT
TCTTGAACTTGCCAATATGGTTGCCGAAACTGTTCATCCAAAAGTGCCGGTGCAGGTGCTCGACACTGGTATAGATGCTG
GAAATTTCAGACGTCGGTATGTCCCCGACATTACGCGCGCATCAAAAGAACTTGGTCTGACTGTTACTGTGCCATTGCAT
GAGGCAATCAAACGTGCCGTTATGGCTGCTCGTGGAGCTGATTGA

Upstream 100 bases:

>100_bases
CAAGAGTAAGCGCCGTTACTATCACCGCGTCAAGTCCGAAGATGAATATCGCATCGAAATGCTCGAAAAAAGGCTTGGTC
AGGCATAGTTACAGTGCCTT

Downstream 100 bases:

>100_bases
TGCAGGCAATAGTATTTGATCTCGATGACCGGCTGGCCTGCCTCCGGGTGTACGAGGAGTATCTGTCAATCAACCGCAAA
CAGCAAGGGAGGTAGCGGTT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MQHQNDESMTIKRLFLTGGTGFFGKSLLRYWIQQSTQGKSVPAVTLMSRSPDAFLASYPEFVGLPWLTLVKGDVTMPKTF
PTELNCSHILHAATDSTNGPMLTPLERFNQIVDGTRNVLDFAHRVEATRFLLTSSGGVYGPQPEKMERIPEDYNGMPDPL
QPSHAYSVAKRTAEHLCALYGDAFGIEILVARCFAFVGPDLPLDAHFAIGNFIADAVAGRQITIKGNGLPIRSYLDQSDL
ATWLLTILEHGQSGRPYNVGSDQAITLLELANMVAETVHPKVPVQVLDTGIDAGNFRRRYVPDITRASKELGLTVTVPLH
EAIKRAVMAARGAD

Sequences:

>Translated_334_residues
MQHQNDESMTIKRLFLTGGTGFFGKSLLRYWIQQSTQGKSVPAVTLMSRSPDAFLASYPEFVGLPWLTLVKGDVTMPKTF
PTELNCSHILHAATDSTNGPMLTPLERFNQIVDGTRNVLDFAHRVEATRFLLTSSGGVYGPQPEKMERIPEDYNGMPDPL
QPSHAYSVAKRTAEHLCALYGDAFGIEILVARCFAFVGPDLPLDAHFAIGNFIADAVAGRQITIKGNGLPIRSYLDQSDL
ATWLLTILEHGQSGRPYNVGSDQAITLLELANMVAETVHPKVPVQVLDTGIDAGNFRRRYVPDITRASKELGLTVTVPLH
EAIKRAVMAARGAD
>Mature_334_residues
MQHQNDESMTIKRLFLTGGTGFFGKSLLRYWIQQSTQGKSVPAVTLMSRSPDAFLASYPEFVGLPWLTLVKGDVTMPKTF
PTELNCSHILHAATDSTNGPMLTPLERFNQIVDGTRNVLDFAHRVEATRFLLTSSGGVYGPQPEKMERIPEDYNGMPDPL
QPSHAYSVAKRTAEHLCALYGDAFGIEILVARCFAFVGPDLPLDAHFAIGNFIADAVAGRQITIKGNGLPIRSYLDQSDL
ATWLLTILEHGQSGRPYNVGSDQAITLLELANMVAETVHPKVPVQVLDTGIDAGNFRRRYVPDITRASKELGLTVTVPLH
EAIKRAVMAARGAD

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=321, Percent_Identity=30.5295950155763, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=23.4939759036145, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=24.1379310344828, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=23.342939481268, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539532, Length=336, Percent_Identity=29.1666666666667, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356223, Length=332, Percent_Identity=29.2168674698795, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36605; Mature: 36605

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHQNDESMTIKRLFLTGGTGFFGKSLLRYWIQQSTQGKSVPAVTLMSRSPDAFLASYPE
CCCCCCCHHEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCHH
FVGLPWLTLVKGDVTMPKTFPTELNCSHILHAATDSTNGPMLTPLERFNQIVDGTRNVLD
HHCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHH
FAHRVEATRFLLTSSGGVYGPQPEKMERIPEDYNGMPDPLQPSHAYSVAKRTAEHLCALY
HHHHHHHHHEEEECCCCCCCCCCHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
GDAFGIEILVARCFAFVGPDLPLDAHFAIGNFIADAVAGRQITIKGNGLPIRSYLDQSDL
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCHHHH
ATWLLTILEHGQSGRPYNVGSDQAITLLELANMVAETVHPKVPVQVLDTGIDAGNFRRRY
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHC
VPDITRASKELGLTVTVPLHEAIKRAVMAARGAD
CCHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQHQNDESMTIKRLFLTGGTGFFGKSLLRYWIQQSTQGKSVPAVTLMSRSPDAFLASYPE
CCCCCCCHHEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCHH
FVGLPWLTLVKGDVTMPKTFPTELNCSHILHAATDSTNGPMLTPLERFNQIVDGTRNVLD
HHCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHH
FAHRVEATRFLLTSSGGVYGPQPEKMERIPEDYNGMPDPLQPSHAYSVAKRTAEHLCALY
HHHHHHHHHEEEECCCCCCCCCCHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
GDAFGIEILVARCFAFVGPDLPLDAHFAIGNFIADAVAGRQITIKGNGLPIRSYLDQSDL
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCHHHH
ATWLLTILEHGQSGRPYNVGSDQAITLLELANMVAETVHPKVPVQVLDTGIDAGNFRRRY
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHC
VPDITRASKELGLTVTVPLHEAIKRAVMAARGAD
CCHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]