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The map label for this gene is asnB [H]

Identifier: 193213071

GI number: 193213071

Start: 1556752

End: 1558530

Strand: Direct

Name: asnB [H]

Synonym: Cpar_1424

Alternate gene names: 193213071

Gene position: 1556752-1558530 (Clockwise)

Preceding gene: 193213068

Following gene: 193213072

Centisome position: 68.0

GC content: 60.71

Gene sequence:

>1779_bases
ATGTGTGGAATTGCCGGAGAGCTGCGTTTCGATGGCGTGGCGGCCTCGTCGGAGGCGGTTGGTCGTATGATCGACATTCT
TGAACCCAGAGGGCCTGACGCCGAGGGCCTTTTCGCTCACAACCGGGTCTGTTTCGGGCACCGGCGGCTGAAAATCATCG
ATCTTTCCGACCACGCTTCGCAGCCGATGGTCGATAGCCAGCTCGGCTTGACGCTGGTCTTCAACGGCGCGATTTACAAC
TACCCCGAATTGCGCGACGAGCTTCAGCAAAAAGGGTACACCTTCTTTTCCCACTCCGATACCGAGGTGATCGTCAAGGC
CTACCACGCATGGGGGCGCGATTGCGTTCTGCACCTGCAGGGCATGTTTGCGTTCGCCGTGTGGGAGCGCGACAGCGGGC
GGGTCTTTTTTGCGCGTGACCGGCTCGGCATCAAGCCGCTTTACTACCACAGGAACAGCTCGCTGATGCGGTTCGCCTCG
TCGCTGCCCGCGCTGGTGAAATCCGGCGGCATCGACACCTCGCTCGGTCCGGTCGCCCTGAACTATTATATGAGCTTCCA
CGCGGTGGTGCCTGCGCCGCACACGATCTTCAACGGCGTCAAAAAGCTTCCACCTGCAACCACGATGACGGTTGAGCCCG
ATGGACAGGCGACCATCGAAACTTACTGGCAGGCCGATTACAGCCGCACCGATGAGGAGCTGAAACGTTCCGAAGAGGAG
TGGATCGACATGGTGTACGAGAGCCTGCTGACGAGCGTCAAACGCCGGTTGGTGGCTGACGTGCCGGTCGGCGTACTGCT
CTCCGGTGGACTGGACTCGTCGCTTGTCGTCGGGTTGCTGAGCGAGCTGGGGCAAAAGCATCTCAACACATTCTCGGTCG
GCTTCGAGGATGTGGCCGAGGAGGAGGGCAACGAGTTCCGCTACTCGGACATCATCGCCGAGCGCTACGCGACGCACCAC
CACAAGATTTTTGTCGATCACCGCGAGTTGCAGACCCACCTCTCCGACTGCATCCGGGCGATGAGTGAGCCGATGGTGAG
CCACGACGTGATCGGCTTCTACCTGCTCTCGCGCGAGGTGAGCAAACACGTCAAGGTGGTGCAGAGCGGGCAGGGAGCGG
ACGAAGTGTTCGCGGGCTACCACTGGTACCCGCCCATGATGGAGGCGACTCCGGCCTCGGCGGTCGAGACCTACCGGAAC
GCCTTTTTCGACCGGGACTTCGACGAGTATTGCCAGGCGGTTTCGTCAAACTACTGCAAGGAGGATTACGCCACGGCGTT
CGTCGCGAACCACTTTGCAGCGCCCGGTGCGGAGGAGCCTATCGATCAGGCGTTGCGGCTCGACAGCAACGTGATGCTGG
TCGATGATCCGGTCAAGCGGGTGGACAACATGACGATGGCCTGGGGACTCGAAGCGCGCGTGCCGTTCCTCGATCACGAG
CTGGTCGAGCTGGCGGCGAAAATCCCTGGCAAGTACAAGGTGCCGGACGGCGGCAAGTATGTGCTGAAAGAGGTCGGGCG
CCGCATCATTCCCCACGAGGTGATCGACCGGCCGAAGGGATACTTCCCTGTTCCGGCGCTGAAGTACCTCGAAGGCGACT
ACCTCGACATGGCCAGAAGCGTGCTCAATCGCGACGTGGCCCGGCAGCGGGGCATCTTCAAAAAAGAGTATGTCGACATG
CTGCTCGATGCGCCGAAGGAACACATCACACCGTTGCGCGGCTCGAAGCTCTGGCAGATCACGCTTCTCGAATACTGGTT
ACAGGAGCAGGGACTATGA

Upstream 100 bases:

>100_bases
AAAGCGCAGAAAAATATTGCGCATTAACAGTGTCAGCTACATCAGGATTGATGCGGCGACCGACCCAGGCGTAATCTGAA
CGATACAGAAGAACATCATA

Downstream 100 bases:

>100_bases
CAAGAAAACACCGAAGTGAAGAGACTCTGGTACCGATGCAGTCCCCCAGCATGAAAAGCTGGGGGCAACCGGCAGAGTTA
CCAAAGCAGAACGTGATCAT

Product: asparagine synthase family amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MCGIAGELRFDGVAASSEAVGRMIDILEPRGPDAEGLFAHNRVCFGHRRLKIIDLSDHASQPMVDSQLGLTLVFNGAIYN
YPELRDELQQKGYTFFSHSDTEVIVKAYHAWGRDCVLHLQGMFAFAVWERDSGRVFFARDRLGIKPLYYHRNSSLMRFAS
SLPALVKSGGIDTSLGPVALNYYMSFHAVVPAPHTIFNGVKKLPPATTMTVEPDGQATIETYWQADYSRTDEELKRSEEE
WIDMVYESLLTSVKRRLVADVPVGVLLSGGLDSSLVVGLLSELGQKHLNTFSVGFEDVAEEEGNEFRYSDIIAERYATHH
HKIFVDHRELQTHLSDCIRAMSEPMVSHDVIGFYLLSREVSKHVKVVQSGQGADEVFAGYHWYPPMMEATPASAVETYRN
AFFDRDFDEYCQAVSSNYCKEDYATAFVANHFAAPGAEEPIDQALRLDSNVMLVDDPVKRVDNMTMAWGLEARVPFLDHE
LVELAAKIPGKYKVPDGGKYVLKEVGRRIIPHEVIDRPKGYFPVPALKYLEGDYLDMARSVLNRDVARQRGIFKKEYVDM
LLDAPKEHITPLRGSKLWQITLLEYWLQEQGL

Sequences:

>Translated_592_residues
MCGIAGELRFDGVAASSEAVGRMIDILEPRGPDAEGLFAHNRVCFGHRRLKIIDLSDHASQPMVDSQLGLTLVFNGAIYN
YPELRDELQQKGYTFFSHSDTEVIVKAYHAWGRDCVLHLQGMFAFAVWERDSGRVFFARDRLGIKPLYYHRNSSLMRFAS
SLPALVKSGGIDTSLGPVALNYYMSFHAVVPAPHTIFNGVKKLPPATTMTVEPDGQATIETYWQADYSRTDEELKRSEEE
WIDMVYESLLTSVKRRLVADVPVGVLLSGGLDSSLVVGLLSELGQKHLNTFSVGFEDVAEEEGNEFRYSDIIAERYATHH
HKIFVDHRELQTHLSDCIRAMSEPMVSHDVIGFYLLSREVSKHVKVVQSGQGADEVFAGYHWYPPMMEATPASAVETYRN
AFFDRDFDEYCQAVSSNYCKEDYATAFVANHFAAPGAEEPIDQALRLDSNVMLVDDPVKRVDNMTMAWGLEARVPFLDHE
LVELAAKIPGKYKVPDGGKYVLKEVGRRIIPHEVIDRPKGYFPVPALKYLEGDYLDMARSVLNRDVARQRGIFKKEYVDM
LLDAPKEHITPLRGSKLWQITLLEYWLQEQGL
>Mature_592_residues
MCGIAGELRFDGVAASSEAVGRMIDILEPRGPDAEGLFAHNRVCFGHRRLKIIDLSDHASQPMVDSQLGLTLVFNGAIYN
YPELRDELQQKGYTFFSHSDTEVIVKAYHAWGRDCVLHLQGMFAFAVWERDSGRVFFARDRLGIKPLYYHRNSSLMRFAS
SLPALVKSGGIDTSLGPVALNYYMSFHAVVPAPHTIFNGVKKLPPATTMTVEPDGQATIETYWQADYSRTDEELKRSEEE
WIDMVYESLLTSVKRRLVADVPVGVLLSGGLDSSLVVGLLSELGQKHLNTFSVGFEDVAEEEGNEFRYSDIIAERYATHH
HKIFVDHRELQTHLSDCIRAMSEPMVSHDVIGFYLLSREVSKHVKVVQSGQGADEVFAGYHWYPPMMEATPASAVETYRN
AFFDRDFDEYCQAVSSNYCKEDYATAFVANHFAAPGAEEPIDQALRLDSNVMLVDDPVKRVDNMTMAWGLEARVPFLDHE
LVELAAKIPGKYKVPDGGKYVLKEVGRRIIPHEVIDRPKGYFPVPALKYLEGDYLDMARSVLNRDVARQRGIFKKEYVDM
LLDAPKEHITPLRGSKLWQITLLEYWLQEQGL

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=563, Percent_Identity=26.8206039076377, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI168229252, Length=563, Percent_Identity=26.8206039076377, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI168229250, Length=563, Percent_Identity=26.8206039076377, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI296010848, Length=534, Percent_Identity=26.9662921348315, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI296010852, Length=471, Percent_Identity=26.1146496815287, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI296010850, Length=471, Percent_Identity=26.1146496815287, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI1786889, Length=547, Percent_Identity=27.9707495429616, Blast_Score=165, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI25147557, Length=524, Percent_Identity=28.0534351145038, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71993933, Length=519, Percent_Identity=27.9383429672447, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560178, Length=554, Percent_Identity=25.812274368231, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI25147560, Length=302, Percent_Identity=28.476821192053, Blast_Score=96, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6325403, Length=583, Percent_Identity=27.4442538593482, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321563, Length=580, Percent_Identity=26.3793103448276, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45553209, Length=369, Percent_Identity=29.5392953929539, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 66890; Mature: 66890

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGELRFDGVAASSEAVGRMIDILEPRGPDAEGLFAHNRVCFGHRRLKIIDLSDHAS
CCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCEEECCCEEEEEECCCCCC
QPMVDSQLGLTLVFNGAIYNYPELRDELQQKGYTFFSHSDTEVIVKAYHAWGRDCVLHLQ
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHH
GMFAFAVWERDSGRVFFARDRLGIKPLYYHRNSSLMRFASSLPALVKSGGIDTSLGPVAL
HHEEEEEEECCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHH
NYYMSFHAVVPAPHTIFNGVKKLPPATTMTVEPDGQATIETYWQADYSRTDEELKRSEEE
HHHHHHHEECCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHCHHCCCHHHHHHHHHH
WIDMVYESLLTSVKRRLVADVPVGVLLSGGLDSSLVVGLLSELGQKHLNTFSVGFEDVAE
HHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EEGNEFRYSDIIAERYATHHHKIFVDHRELQTHLSDCIRAMSEPMVSHDVIGFYLLSREV
HCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SKHVKVVQSGQGADEVFAGYHWYPPMMEATPASAVETYRNAFFDRDFDEYCQAVSSNYCK
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
EDYATAFVANHFAAPGAEEPIDQALRLDSNVMLVDDPVKRVDNMTMAWGLEARVPFLDHE
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEECCHHHHHCCCEEEECCCCCCCCHHHH
LVELAAKIPGKYKVPDGGKYVLKEVGRRIIPHEVIDRPKGYFPVPALKYLEGDYLDMARS
HHHHHHHCCCCEECCCCHHHHHHHHCHHCCCHHHHCCCCCCCCCCHHHHHCCHHHHHHHH
VLNRDVARQRGIFKKEYVDMLLDAPKEHITPLRGSKLWQITLLEYWLQEQGL
HHHHHHHHHHCCHHHHHHHHHHHCCHHHCCCCCCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MCGIAGELRFDGVAASSEAVGRMIDILEPRGPDAEGLFAHNRVCFGHRRLKIIDLSDHAS
CCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCEEECCCEEEEEECCCCCC
QPMVDSQLGLTLVFNGAIYNYPELRDELQQKGYTFFSHSDTEVIVKAYHAWGRDCVLHLQ
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHH
GMFAFAVWERDSGRVFFARDRLGIKPLYYHRNSSLMRFASSLPALVKSGGIDTSLGPVAL
HHEEEEEEECCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHH
NYYMSFHAVVPAPHTIFNGVKKLPPATTMTVEPDGQATIETYWQADYSRTDEELKRSEEE
HHHHHHHEECCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHCHHCCCHHHHHHHHHH
WIDMVYESLLTSVKRRLVADVPVGVLLSGGLDSSLVVGLLSELGQKHLNTFSVGFEDVAE
HHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EEGNEFRYSDIIAERYATHHHKIFVDHRELQTHLSDCIRAMSEPMVSHDVIGFYLLSREV
HCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SKHVKVVQSGQGADEVFAGYHWYPPMMEATPASAVETYRNAFFDRDFDEYCQAVSSNYCK
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
EDYATAFVANHFAAPGAEEPIDQALRLDSNVMLVDDPVKRVDNMTMAWGLEARVPFLDHE
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEECCHHHHHCCCEEEECCCCCCCCHHHH
LVELAAKIPGKYKVPDGGKYVLKEVGRRIIPHEVIDRPKGYFPVPALKYLEGDYLDMARS
HHHHHHHCCCCEECCCCHHHHHHHHCHHCCCHHHHCCCCCCCCCCHHHHHCCHHHHHHHH
VLNRDVARQRGIFKKEYVDMLLDAPKEHITPLRGSKLWQITLLEYWLQEQGL
HHHHHHHHHHCCHHHHHHHHHHHCCHHHCCCCCCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]