Click here to switch to the map view.

The map label for this gene is phoA [H]

Identifier: 192288827

GI number: 192288827

Start: 423796

End: 425553

Strand: Reverse

Name: phoA [H]

Synonym: Rpal_0396

Alternate gene names: 192288827

Gene position: 425553-423796 (Counterclockwise)

Preceding gene: 192288828

Following gene: 192288825

Centisome position: 7.41

GC content: 66.72

Gene sequence:

>1758_bases
ATGATGACCAAGCATATTGTCGCGACGGCGTTTGCGTCGCTGATGTTCGTTTCGGCCGCTTCGGCCCAAACCATTTATCC
AATCGACCGCGCCGAGATCCTGGCCGGCACCAGGTTCGACCTCAAGGTCGAATTCGCCGGCGAAGTCACCGCGGATAAAG
CCAGCCTCACCATCAATGGCGCTGACGCCGCGAGCGTGTTCGGCAAGGCACCGGAGCTGATCGCGCAAGAAGCGGGGCAG
CCGCGATCGGCGCTGCTGCTGCGCGACGTGACGCTGAACAAACCCGGCCGCTACACCGTCGAAGCCGGTGACGGCGCTGT
GCGCCGCAGCGTGAGCTGGACGGTGTACGACACCGGGCCACGCCGGGCCAAGAACGTGATCCTGTTTATCGGCGACGGAC
TGTCGCCGGCGCATCGGGTGGCGGCACGACTGCTGTCGAAAGGCATTCAGGAAGGCCGTGCGGGCGGCAAGCTGGCGATC
GACGATATGCCGCAGATGGCGCTGGTCTCGACCGCGGGCAGCGACTCGATCATCACCGACTCGGCCAATGCGGCCAGCGC
CTACGCGACCGGCCATAAGGCCGCCGTCAACGCGATGGGGGTCTATGCGGACCGCACCCCCGACCCGCTCGACGACCCCA
AGGTGGAAACGCTTGCGTCGGTCGCGAAGCGGCGGCTGGGGCTGTCGATCGGCATCGTCACCAACACCGAAGTGGAAGAC
GCGACGCCCGCCGCGGTGATCGCCCACACCCGCCGCCGCGCGGCTTATGACGACATCGTCGCGCAGTTCTATGCCGCGCA
GCCCGACGTGCTGATGGGCGGCGGCGCCGCTTACTTCCTGCCAAAGAGCGCGAACGGCAAGCGCAAGGATGATATCGATT
ATCTGGCGAAGTTCCGCGACGCCGGCTACGCGGTCGCCAACAATGCGACCGACCTCGACGCGGCGAGCAAGACCGCCCCG
CGAAAACTGCTCGGCCTGTTCGCTCCCGGCAACATGGACGGCGTGCTCGACCGCCGTTTCCTGAAAGGTGGTGGCGTCAC
CAAGAACCCCGACCAGCCTGATCTGACCCAGCAGGTCGCGGTCGCGCTCGACGTGCTGTCGAAGAACGATGCGGGCTTCT
TCCTGATGGTCGAATCCGGCCTGATCGACAAATACGCCCATGCGCTCGACATGGAGCGAGCCGTCTATGACACCATCATG
CTCGACAACGCGGTGCGCCAGACCCGCGACTGGGCCAAGGCCCGCGGCGACGACACGCTGATCCTGGTGGTGGCCGATCA
CAACCATCCCAATGCGCTGGTCGGCACCGTGCGCGACGATCTCGCCGAGGGTGACGACGTGCCGCTGCGCGAGCGGATCG
GCGTGTACGAACGCGCCGGCTTTCCGAACTATCCGGCGCCCGATGCGGACGGCTATCCGGACCGCGTCGATGTCAGCCGG
CGCATCGCGATCTTCTCGGCCAGCCTTCCGGATCACTACGAGACGCTGCGGCCGAAGCTCGACGGGCCGAACCAGCCGAC
CGAGGCGGGCGACAAGCACGGCACCTTCAAGGCCAACGACAAGTACAAGGGCGTGCCCGGGGCGGTGCTGCGCATCGGCA
ATCTGCCGGCGATGATGAATGCCAGCGTGCACTCCGGCGAGGACGTGATCCTGACCGCTACCGGCCCCGGCAGCGACCGC
GTTCGCGGCTCGCTGGAGAACACCGACGTGTTCCGGGTGATGGCCGCCGCGCTGGGCCTCGGCGCCGAGGCCAAGTAG

Upstream 100 bases:

>100_bases
TGGTTGGTTGCTAAGATCGAGCAACATCCATCGCCACCCGCCTTCGTCATCCAACCGTCATTTGCCGCTCATACGGTCCG
CGCGCCAATCAGGACCGTTT

Downstream 100 bases:

>100_bases
CAACGACGCGAGGTCTAAGCCACCCGCGCCGATCCCATCGCAAACTCTGCACCGGCGGAGGCACCGCTCTGCTGCAGGTC
GAGCAGCCCGGTGATTTCGG

Product: Alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MMTKHIVATAFASLMFVSAASAQTIYPIDRAEILAGTRFDLKVEFAGEVTADKASLTINGADAASVFGKAPELIAQEAGQ
PRSALLLRDVTLNKPGRYTVEAGDGAVRRSVSWTVYDTGPRRAKNVILFIGDGLSPAHRVAARLLSKGIQEGRAGGKLAI
DDMPQMALVSTAGSDSIITDSANAASAYATGHKAAVNAMGVYADRTPDPLDDPKVETLASVAKRRLGLSIGIVTNTEVED
ATPAAVIAHTRRRAAYDDIVAQFYAAQPDVLMGGGAAYFLPKSANGKRKDDIDYLAKFRDAGYAVANNATDLDAASKTAP
RKLLGLFAPGNMDGVLDRRFLKGGGVTKNPDQPDLTQQVAVALDVLSKNDAGFFLMVESGLIDKYAHALDMERAVYDTIM
LDNAVRQTRDWAKARGDDTLILVVADHNHPNALVGTVRDDLAEGDDVPLRERIGVYERAGFPNYPAPDADGYPDRVDVSR
RIAIFSASLPDHYETLRPKLDGPNQPTEAGDKHGTFKANDKYKGVPGAVLRIGNLPAMMNASVHSGEDVILTATGPGSDR
VRGSLENTDVFRVMAAALGLGAEAK

Sequences:

>Translated_585_residues
MMTKHIVATAFASLMFVSAASAQTIYPIDRAEILAGTRFDLKVEFAGEVTADKASLTINGADAASVFGKAPELIAQEAGQ
PRSALLLRDVTLNKPGRYTVEAGDGAVRRSVSWTVYDTGPRRAKNVILFIGDGLSPAHRVAARLLSKGIQEGRAGGKLAI
DDMPQMALVSTAGSDSIITDSANAASAYATGHKAAVNAMGVYADRTPDPLDDPKVETLASVAKRRLGLSIGIVTNTEVED
ATPAAVIAHTRRRAAYDDIVAQFYAAQPDVLMGGGAAYFLPKSANGKRKDDIDYLAKFRDAGYAVANNATDLDAASKTAP
RKLLGLFAPGNMDGVLDRRFLKGGGVTKNPDQPDLTQQVAVALDVLSKNDAGFFLMVESGLIDKYAHALDMERAVYDTIM
LDNAVRQTRDWAKARGDDTLILVVADHNHPNALVGTVRDDLAEGDDVPLRERIGVYERAGFPNYPAPDADGYPDRVDVSR
RIAIFSASLPDHYETLRPKLDGPNQPTEAGDKHGTFKANDKYKGVPGAVLRIGNLPAMMNASVHSGEDVILTATGPGSDR
VRGSLENTDVFRVMAAALGLGAEAK
>Mature_585_residues
MMTKHIVATAFASLMFVSAASAQTIYPIDRAEILAGTRFDLKVEFAGEVTADKASLTINGADAASVFGKAPELIAQEAGQ
PRSALLLRDVTLNKPGRYTVEAGDGAVRRSVSWTVYDTGPRRAKNVILFIGDGLSPAHRVAARLLSKGIQEGRAGGKLAI
DDMPQMALVSTAGSDSIITDSANAASAYATGHKAAVNAMGVYADRTPDPLDDPKVETLASVAKRRLGLSIGIVTNTEVED
ATPAAVIAHTRRRAAYDDIVAQFYAAQPDVLMGGGAAYFLPKSANGKRKDDIDYLAKFRDAGYAVANNATDLDAASKTAP
RKLLGLFAPGNMDGVLDRRFLKGGGVTKNPDQPDLTQQVAVALDVLSKNDAGFFLMVESGLIDKYAHALDMERAVYDTIM
LDNAVRQTRDWAKARGDDTLILVVADHNHPNALVGTVRDDLAEGDDVPLRERIGVYERAGFPNYPAPDADGYPDRVDVSR
RIAIFSASLPDHYETLRPKLDGPNQPTEAGDKHGTFKANDKYKGVPGAVLRIGNLPAMMNASVHSGEDVILTATGPGSDR
VRGSLENTDVFRVMAAALGLGAEAK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=341, Percent_Identity=33.4310850439883, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI116734717, Length=504, Percent_Identity=29.1666666666667, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI157266292, Length=342, Percent_Identity=33.6257309941521, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI94721246, Length=341, Percent_Identity=33.1378299120235, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI294660770, Length=340, Percent_Identity=31.4705882352941, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI294660772, Length=462, Percent_Identity=27.4891774891775, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI48994877, Length=357, Percent_Identity=33.6134453781513, Blast_Score=146, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320689, Length=337, Percent_Identity=29.3768545994065, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21355981, Length=493, Percent_Identity=27.9918864097363, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21358067, Length=509, Percent_Identity=29.2730844793713, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI85725200, Length=393, Percent_Identity=29.2620865139949, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI85815833, Length=393, Percent_Identity=29.2620865139949, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24657835, Length=499, Percent_Identity=28.2565130260521, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21355151, Length=553, Percent_Identity=26.5822784810127, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24585213, Length=358, Percent_Identity=31.8435754189944, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24657827, Length=501, Percent_Identity=28.3433133732535, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24657842, Length=502, Percent_Identity=27.6892430278884, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355149, Length=503, Percent_Identity=28.4294234592445, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18859923, Length=482, Percent_Identity=26.7634854771784, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI19921912, Length=501, Percent_Identity=27.5449101796407, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24651554, Length=512, Percent_Identity=25.9765625, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 62188; Mature: 62188

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTKHIVATAFASLMFVSAASAQTIYPIDRAEILAGTRFDLKVEFAGEVTADKASLTING
CCCHHHHHHHHHHHHHHHHCCCCEECCCCHHHHEECCEEEEEEEECCCEECCCEEEEECC
ADAASVFGKAPELIAQEAGQPRSALLLRDVTLNKPGRYTVEAGDGAVRRSVSWTVYDTGP
CCHHHHHCCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCCCCEEEEEEEEEEECCC
RRAKNVILFIGDGLSPAHRVAARLLSKGIQEGRAGGKLAIDDMPQMALVSTAGSDSIITD
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCEEEC
SANAASAYATGHKAAVNAMGVYADRTPDPLDDPKVETLASVAKRRLGLSIGIVTNTEVED
CCCCCHHHHCCCHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCC
ATPAAVIAHTRRRAAYDDIVAQFYAAQPDVLMGGGAAYFLPKSANGKRKDDIDYLAKFRD
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHH
AGYAVANNATDLDAASKTAPRKLLGLFAPGNMDGVLDRRFLKGGGVTKNPDQPDLTQQVA
CCEEEECCCCCCCCHHHCCCHHHEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHH
VALDVLSKNDAGFFLMVESGLIDKYAHALDMERAVYDTIMLDNAVRQTRDWAKARGDDTL
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ILVVADHNHPNALVGTVRDDLAEGDDVPLRERIGVYERAGFPNYPAPDADGYPDRVDVSR
EEEEECCCCCCCEEEHHHHHHCCCCCCCHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCE
RIAIFSASLPDHYETLRPKLDGPNQPTEAGDKHGTFKANDKYKGVPGAVLRIGNLPAMMN
EEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHEEEECCCCCEEC
ASVHSGEDVILTATGPGSDRVRGSLENTDVFRVMAAALGLGAEAK
CCCCCCCEEEEEECCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMTKHIVATAFASLMFVSAASAQTIYPIDRAEILAGTRFDLKVEFAGEVTADKASLTING
CCCHHHHHHHHHHHHHHHHCCCCEECCCCHHHHEECCEEEEEEEECCCEECCCEEEEECC
ADAASVFGKAPELIAQEAGQPRSALLLRDVTLNKPGRYTVEAGDGAVRRSVSWTVYDTGP
CCHHHHHCCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCCCCEEEEEEEEEEECCC
RRAKNVILFIGDGLSPAHRVAARLLSKGIQEGRAGGKLAIDDMPQMALVSTAGSDSIITD
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCEEEC
SANAASAYATGHKAAVNAMGVYADRTPDPLDDPKVETLASVAKRRLGLSIGIVTNTEVED
CCCCCHHHHCCCHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCC
ATPAAVIAHTRRRAAYDDIVAQFYAAQPDVLMGGGAAYFLPKSANGKRKDDIDYLAKFRD
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHH
AGYAVANNATDLDAASKTAPRKLLGLFAPGNMDGVLDRRFLKGGGVTKNPDQPDLTQQVA
CCEEEECCCCCCCCHHHCCCHHHEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHH
VALDVLSKNDAGFFLMVESGLIDKYAHALDMERAVYDTIMLDNAVRQTRDWAKARGDDTL
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ILVVADHNHPNALVGTVRDDLAEGDDVPLRERIGVYERAGFPNYPAPDADGYPDRVDVSR
EEEEECCCCCCCEEEHHHHHHCCCCCCCHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCE
RIAIFSASLPDHYETLRPKLDGPNQPTEAGDKHGTFKANDKYKGVPGAVLRIGNLPAMMN
EEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHEEEECCCCCEEC
ASVHSGEDVILTATGPGSDRVRGSLENTDVFRVMAAALGLGAEAK
CCCCCCCEEEEEECCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]