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The map label for this gene is mepA [H]

Identifier: 192288825

GI number: 192288825

Start: 420201

End: 421163

Strand: Reverse

Name: mepA [H]

Synonym: Rpal_0394

Alternate gene names: 192288825

Gene position: 421163-420201 (Counterclockwise)

Preceding gene: 192288827

Following gene: 192288823

Centisome position: 7.33

GC content: 68.22

Gene sequence:

>963_bases
ATGCGCCGCGCGATTCTTGCTGCTCCGGTCCGGCTCGCCAGCTTACTTGCTGCGATGATGTCGCTCGTTCCGGCCGCCGT
GGCCCAGGACAAAGGCACGCTCGATCCCAAACCGCTGCCTCCGCTGGCGCATCCGGACGATCCCAATCTCGCAGCACGCG
AGCTGTTCGCCCGCAAGCTGCGCCCAACCGCGCAACAGCCGCGCCCGATCGGCTTCTACGCCAAGGGCTGCATGGCCGGC
GGCATCTCGTTACCTATCAACGGGCCGAGCTGGCAGGTGATGCGCCTGTCGCGCAATCGGGCGTGGGCGCTTCCCGCCAC
CGTACGGCTAATCGAGCGCGTCGCCGACAAGGCGCGCGAGCAGGCGGGCTGGAACGGTATCCTGGTCGGCGACATGTCCC
AGCCGCGCGGCGGGCCGATGCTGACCGGCCACGCCAGCCACCAGGTCGGGCTCGACGCCGATATCTGGCTGACGCCGATG
CCGAACCGGCAATTGTCGCGCAACGAGCGCGAAGAGATGTCGGCGGTGATGATGGTCCGCAGCGACCGGCTGGACATTGA
GCCGTCGGCCTGGACGCCGACGCATTGGAAGGTGATCCGCGCCGCGGCGCTGGAGCCACAGGTGGACCGGATCTTCGTCA
ACGCTGCGATCAAGAAGGCGCTGTGCCGCGAGGCCAAAGGCGACCGCAGCTGGCTGCACAAGGTGCGGCCGATGTACGGC
CACGATTACCACTTCCACATCCGGCTGCGCTGCCCCGCCGGTATCGAGGGCTGCGAAGCGCAGGCCGAACCGCCGCCGGG
CGAAGGCTGCGATGCCTCCGACTTCGCCTACTGGTTCAGCGACGCAGTGCTGCATCCGAAGCCGCCGCTCAAGCCGCCTC
CGCCGCGGCATCAGCTCACGCTGTCGGAACTGCCGGCCGAATGCCGGACGGTGCTGAACGCGCCCGACAAAAAGCCGGAC
TAA

Upstream 100 bases:

>100_bases
TTAAGAACGGACGAATTCAGCGGAAATCATCCCCGCTTCGCCAAACCAAGGCCCCAAGCCCACCGGTCGAGATCAAGCTC
GCGTGCGGTATAGTGATGGA

Downstream 100 bases:

>100_bases
TCACTTCGTTTGAGTTGTTCCAGGCTACACGCCGGCCAGCAACGACCAGACCTCCGACAGCTTGGTCCTGACTTTGGCGA
GCCGGGTCGGCGGCGGAGCT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRRAILAAPVRLASLLAAMMSLVPAAVAQDKGTLDPKPLPPLAHPDDPNLAARELFARKLRPTAQQPRPIGFYAKGCMAG
GISLPINGPSWQVMRLSRNRAWALPATVRLIERVADKAREQAGWNGILVGDMSQPRGGPMLTGHASHQVGLDADIWLTPM
PNRQLSRNEREEMSAVMMVRSDRLDIEPSAWTPTHWKVIRAAALEPQVDRIFVNAAIKKALCREAKGDRSWLHKVRPMYG
HDYHFHIRLRCPAGIEGCEAQAEPPPGEGCDASDFAYWFSDAVLHPKPPLKPPPPRHQLTLSELPAECRTVLNAPDKKPD

Sequences:

>Translated_320_residues
MRRAILAAPVRLASLLAAMMSLVPAAVAQDKGTLDPKPLPPLAHPDDPNLAARELFARKLRPTAQQPRPIGFYAKGCMAG
GISLPINGPSWQVMRLSRNRAWALPATVRLIERVADKAREQAGWNGILVGDMSQPRGGPMLTGHASHQVGLDADIWLTPM
PNRQLSRNEREEMSAVMMVRSDRLDIEPSAWTPTHWKVIRAAALEPQVDRIFVNAAIKKALCREAKGDRSWLHKVRPMYG
HDYHFHIRLRCPAGIEGCEAQAEPPPGEGCDASDFAYWFSDAVLHPKPPLKPPPPRHQLTLSELPAECRTVLNAPDKKPD
>Mature_320_residues
MRRAILAAPVRLASLLAAMMSLVPAAVAQDKGTLDPKPLPPLAHPDDPNLAARELFARKLRPTAQQPRPIGFYAKGCMAG
GISLPINGPSWQVMRLSRNRAWALPATVRLIERVADKAREQAGWNGILVGDMSQPRGGPMLTGHASHQVGLDADIWLTPM
PNRQLSRNEREEMSAVMMVRSDRLDIEPSAWTPTHWKVIRAAALEPQVDRIFVNAAIKKALCREAKGDRSWLHKVRPMYG
HDYHFHIRLRCPAGIEGCEAQAEPPPGEGCDASDFAYWFSDAVLHPKPPLKPPPPRHQLTLSELPAECRTVLNAPDKKPD

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=254, Percent_Identity=38.1889763779528, Blast_Score=168, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 35382; Mature: 35382

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAILAAPVRLASLLAAMMSLVPAAVAQDKGTLDPKPLPPLAHPDDPNLAARELFARKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RPTAQQPRPIGFYAKGCMAGGISLPINGPSWQVMRLSRNRAWALPATVRLIERVADKARE
CCCCCCCCCCCCEEHHHHCCCEEECCCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHH
QAGWNGILVGDMSQPRGGPMLTGHASHQVGLDADIWLTPMPNRQLSRNEREEMSAVMMVR
HCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCEEECCCCCHHHCHHHHHHHHHHHHHH
SDRLDIEPSAWTPTHWKVIRAAALEPQVDRIFVNAAIKKALCREAKGDRSWLHKVRPMYG
CCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHCC
HDYHFHIRLRCPAGIEGCEAQAEPPPGEGCDASDFAYWFSDAVLHPKPPLKPPPPRHQLT
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
LSELPAECRTVLNAPDKKPD
HHHCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRRAILAAPVRLASLLAAMMSLVPAAVAQDKGTLDPKPLPPLAHPDDPNLAARELFARKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RPTAQQPRPIGFYAKGCMAGGISLPINGPSWQVMRLSRNRAWALPATVRLIERVADKARE
CCCCCCCCCCCCEEHHHHCCCEEECCCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHH
QAGWNGILVGDMSQPRGGPMLTGHASHQVGLDADIWLTPMPNRQLSRNEREEMSAVMMVR
HCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCEEECCCCCHHHCHHHHHHHHHHHHHH
SDRLDIEPSAWTPTHWKVIRAAALEPQVDRIFVNAAIKKALCREAKGDRSWLHKVRPMYG
CCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHCC
HDYHFHIRLRCPAGIEGCEAQAEPPPGEGCDASDFAYWFSDAVLHPKPPLKPPPPRHQLT
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
LSELPAECRTVLNAPDKKPD
HHHCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]