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The map label for this gene is argG [H]

Identifier: 192288828

GI number: 192288828

Start: 425794

End: 427131

Strand: Reverse

Name: argG [H]

Synonym: Rpal_0397

Alternate gene names: 192288828

Gene position: 427131-425794 (Counterclockwise)

Preceding gene: 192288829

Following gene: 192288827

Centisome position: 7.44

GC content: 64.95

Gene sequence:

>1338_bases
ATGACTACGATCTTGAAAAGCCTGCCCAAGGGCGAAAACGTCGGCATCGCTTTCTCAGGGGGTCTCGATACCAGCGCGGC
GCTGCTGTGGATGAAGCAGAAGGGCGCCAAGGTGTTCGCCTATACGGCGAACCTCGGCCAGCCCGACGAGGCCGACTACG
ACGCGATCCCGCGCAAGGCGATGGAGTTCGGCGCCGAGAAGGCCCGGCTGGTCGACTGCCGCACCCAGCTGGTGCACGAA
GGCATCGCGGCGATCCAGGCCGGCGCCTTCCACGTCTCGACCGGCGGCATCGCGTATTTCAACACCACCCCGCTCGGCCG
CGCCGTGACCGGCACCATGCTGGTGTCGGCGATGAAGGAAGACGGCGTCAACATCTGGGGCGACGGCTCGACCTACAAGG
GCAACGACATCGAGCGGTTCTACCGCTACGGCCTGCTCACCAATCCGGCCTTGCGGATCTACAAGCCCTGGCTCGACCAG
CAGTTCATCGACGAGCTCGGCGGCCGCGCCGAGATGTCGGCATTCATGACCTCGCACGGCTTCGCCTACAAGATGAGCGC
CGAAAAGGCGTACTCGACCGACAGCAACCTGCTCGGCGCCACCCACGAGGCCAAGGATCTCGAACACCTCGACAGCGGCA
TCAAGATCGTCAACCCGATCATGGGCGTGCCGTTCTGGCGCGACGATTGCGCGGTGAAAGCCGAAGCCGTCACGGTGCGG
TTCGAGGAAGGCCAGCCGGTCGCGCTCAATGGCCAGACTTTCACGGATCCGGTCGCGATGTTCTACGAAGCCAATGCCAT
TGGCGGACGTCACGGCCTCGGCATGAGCGACCAGATCGAGAACCGCATCATCGAGGCCAAGAGCCGCGGCATCTACGAGG
CGCCCGGCATGGCGCTGCTGCACATCGCCTATGAGCGGCTGGTCACCGGCATTCACAACGAAGACACCATCGAGCAGTAC
CGCATCAACGGCATGAAGCTCGGCCGGCTGCTGTATCAGGGCCGCTGGTTCGACTCACAGGCTTTGATGCTGCGCGAGAC
CGCGCAGCGCTGGGTCGCCCGTGCCGTCACCGGCGAGGTGACGCTCGAACTGCGCCGCGGCAACGACTACTCGATCCTCA
ACACCGTCAGCCCGAACCTGACCTACGCGCCGGAGCGGCTGAGCATGGAGAAGGTCGAGGACGCGCCGTTCACGCCGGCC
GACCGCATCGGCCAGCTCACCATGCGCAACCTCGACATCACCGACACCCGCGCCAAGCTCGGCCTCTACGCCAAGACCGG
CCTGCTGTCCTCCGGCGAAGGCACCCCGATTCCGCAGCTGGAAAACGACAAGGGCTGA

Upstream 100 bases:

>100_bases
GCCGCATCCGCACCTCCGCCGCGATTAAACGCGTGCCGCTAGCCGCGAAGTTTGATATGCGCGCAGCAACCGCAACTGCT
TCAACCGATTGGGACTTCCA

Downstream 100 bases:

>100_bases
GGGCCTTCACGGTCATTCCGGGGCGCTCACGGCAGTGAGCGAACCCGGAATCCCGAGATTGTTTGGCTGTCTCGGTGCCC
CGAACTTCGAGATTTCCGGT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTTILKSLPKGENVGIAFSGGLDTSAALLWMKQKGAKVFAYTANLGQPDEADYDAIPRKAMEFGAEKARLVDCRTQLVHE
GIAAIQAGAFHVSTGGIAYFNTTPLGRAVTGTMLVSAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNPALRIYKPWLDQ
QFIDELGGRAEMSAFMTSHGFAYKMSAEKAYSTDSNLLGATHEAKDLEHLDSGIKIVNPIMGVPFWRDDCAVKAEAVTVR
FEEGQPVALNGQTFTDPVAMFYEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQY
RINGMKLGRLLYQGRWFDSQALMLRETAQRWVARAVTGEVTLELRRGNDYSILNTVSPNLTYAPERLSMEKVEDAPFTPA
DRIGQLTMRNLDITDTRAKLGLYAKTGLLSSGEGTPIPQLENDKG

Sequences:

>Translated_445_residues
MTTILKSLPKGENVGIAFSGGLDTSAALLWMKQKGAKVFAYTANLGQPDEADYDAIPRKAMEFGAEKARLVDCRTQLVHE
GIAAIQAGAFHVSTGGIAYFNTTPLGRAVTGTMLVSAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNPALRIYKPWLDQ
QFIDELGGRAEMSAFMTSHGFAYKMSAEKAYSTDSNLLGATHEAKDLEHLDSGIKIVNPIMGVPFWRDDCAVKAEAVTVR
FEEGQPVALNGQTFTDPVAMFYEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQY
RINGMKLGRLLYQGRWFDSQALMLRETAQRWVARAVTGEVTLELRRGNDYSILNTVSPNLTYAPERLSMEKVEDAPFTPA
DRIGQLTMRNLDITDTRAKLGLYAKTGLLSSGEGTPIPQLENDKG
>Mature_444_residues
TTILKSLPKGENVGIAFSGGLDTSAALLWMKQKGAKVFAYTANLGQPDEADYDAIPRKAMEFGAEKARLVDCRTQLVHEG
IAAIQAGAFHVSTGGIAYFNTTPLGRAVTGTMLVSAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNPALRIYKPWLDQQ
FIDELGGRAEMSAFMTSHGFAYKMSAEKAYSTDSNLLGATHEAKDLEHLDSGIKIVNPIMGVPFWRDDCAVKAEAVTVRF
EEGQPVALNGQTFTDPVAMFYEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYR
INGMKLGRLLYQGRWFDSQALMLRETAQRWVARAVTGEVTLELRRGNDYSILNTVSPNLTYAPERLSMEKVEDAPFTPAD
RIGQLTMRNLDITDTRAKLGLYAKTGLLSSGEGTPIPQLENDKG

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=415, Percent_Identity=29.3975903614458, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI16950633, Length=415, Percent_Identity=29.3975903614458, Blast_Score=155, Evalue=5e-38,
Organism=Escherichia coli, GI1789563, Length=442, Percent_Identity=80.0904977375566, Blast_Score=734, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=421, Percent_Identity=29.2161520190024, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21358151, Length=429, Percent_Identity=28.6713286713287, Blast_Score=171, Evalue=8e-43,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 48969; Mature: 48838

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTILKSLPKGENVGIAFSGGLDTSAALLWMKQKGAKVFAYTANLGQPDEADYDAIPRKA
CCCHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHCCHHH
MEFGAEKARLVDCRTQLVHEGIAAIQAGAFHVSTGGIAYFNTTPLGRAVTGTMLVSAMKE
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHH
DGVNIWGDGSTYKGNDIERFYRYGLLTNPALRIYKPWLDQQFIDELGGRAEMSAFMTSHG
CCCEEECCCCCCCCCHHHHHHHHCCCCCCCHHEEHHHCCHHHHHHHCCHHHHHHHHHCCC
FAYKMSAEKAYSTDSNLLGATHEAKDLEHLDSGIKIVNPIMGVPFWRDDCAVKAEAVTVR
EEEEECCCHHCCCCCCCCCCCCHHHHHHHHHCCCEEHHHHHCCCCCCCCCCEEEEEEEEE
FEEGQPVALNGQTFTDPVAMFYEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALL
ECCCCEEEECCCCCCCHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
HIAYERLVTGIHNEDTIEQYRINGMKLGRLLYQGRWFDSQALMLRETAQRWVARAVTGEV
HHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEE
TLELRRGNDYSILNTVSPNLTYAPERLSMEKVEDAPFTPADRIGQLTMRNLDITDTRAKL
EEEEECCCCEEEEEECCCCCEECCCCCCHHHHCCCCCCCHHHHCCEEECCCCCCCCHHHH
GLYAKTGLLSSGEGTPIPQLENDKG
EEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTILKSLPKGENVGIAFSGGLDTSAALLWMKQKGAKVFAYTANLGQPDEADYDAIPRKA
CCHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHCCHHH
MEFGAEKARLVDCRTQLVHEGIAAIQAGAFHVSTGGIAYFNTTPLGRAVTGTMLVSAMKE
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHH
DGVNIWGDGSTYKGNDIERFYRYGLLTNPALRIYKPWLDQQFIDELGGRAEMSAFMTSHG
CCCEEECCCCCCCCCHHHHHHHHCCCCCCCHHEEHHHCCHHHHHHHCCHHHHHHHHHCCC
FAYKMSAEKAYSTDSNLLGATHEAKDLEHLDSGIKIVNPIMGVPFWRDDCAVKAEAVTVR
EEEEECCCHHCCCCCCCCCCCCHHHHHHHHHCCCEEHHHHHCCCCCCCCCCEEEEEEEEE
FEEGQPVALNGQTFTDPVAMFYEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALL
ECCCCEEEECCCCCCCHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
HIAYERLVTGIHNEDTIEQYRINGMKLGRLLYQGRWFDSQALMLRETAQRWVARAVTGEV
HHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEE
TLELRRGNDYSILNTVSPNLTYAPERLSMEKVEDAPFTPADRIGQLTMRNLDITDTRAKL
EEEEECCCCEEEEEECCCCCEECCCCCCHHHHCCCCCCCHHHHCCEEECCCCCCCCHHHH
GLYAKTGLLSSGEGTPIPQLENDKG
EEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA