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The map label for this gene is dadX [H]

Identifier: 190894994

GI number: 190894994

Start: 713536

End: 714669

Strand: Reverse

Name: dadX [H]

Synonym: RHECIAT_PC0000660

Alternate gene names: 190894994

Gene position: 714669-713536 (Counterclockwise)

Preceding gene: 190894996

Following gene: 190894993

Centisome position: 65.47

GC content: 63.93

Gene sequence:

>1134_bases
ATGGATAGCGACATTTTGGGTTCCCGCGAACGCACCGTTACGATCACGCAGGGAGCCACCGGCTATCTGACGATCGATCT
TGCCGCACTCGGCCGCAATTATTTGAAGCTCGCGTCGATGCTGGCGCCGGTCCGCGCGGGAGCCGTCGTCAAGGCCGACG
CTTATGGTCTCGGCGCCGAACGGGTCGCCCAAACGCTCTATGATCAGGGCTGCAGGCATTTCTTCGTCGCCCAGTTCGTC
GAGGCCGTGAGGCTGCGGCCAGCCCTTGCCGAGGATGCCCAGATATTCGTGCTGAACGGCTTGCAGCCAGGCAACGAGGT
CGCCTGCGCCGAAAGCGGTATCATTCCGGTTCTCAATTCGCTCGCGCAGTGGCGGCAATGGTCGGCGACCGCGCGCTTGC
TGACGCGCAGCCTGCCGGCCGTTCTGCAGTTTGACACCGGCATGTCGCGGCTCGGCTTTCCCTGGGAAGAGCGAGCCGAA
TTGGCGGCAGTCATCGGCGACGGCGCCAATGTCGAGATCCTGTTCATCATGAGCCACCTGGCCTGTGCCGACGAGCTGGA
TAGCGGCCAGAACGGCGACCAGCTTGCCGAGATGGCGCGCATTGCCGATGAATTTCCGGGCTTCGACATCTCCTTCGCCA
ATTCCGGCGGCGTCTTTCTCGGCGATGCCTATCATGGCGTGCTCGCCCGCCCGGGCATCGCCCTTTATGGCGGCGCACCG
AACGCCGGCGGCACGAACCCGATGGAACCGGTCGTCAGGCTGGACGTCGCCGTCGTGCAGACGCGCACCGTACCTTCGGG
CGCCAGGATCGGTTATGGCGGCGCGCATGTGACGCGCAGAGAAACCCGCCTTGCCACCATCGCCGCCGGCTATGCCGACG
GCCTGCCGCGCAGTCTCGGCGATTGCGGCGCCGTCTATCACAAGGGTATCCGTCTGCCGATCGTCGGGCGCGTGTCGATG
GACAGCGCGACTGTCGATATCACGGCTTTGCCCGAGGGGGCGCTGAGGCTTGGAAGCCTTGTCGAAGTGCTTGGCCCGAA
CCAAACGCTGGAAGACGTGGCTGGCGCTGCCGGAACGATATCTTACGAAATCCTAACTGGTCTGGGCGATCGTTACGACA
GGCAATATTGCTGA

Upstream 100 bases:

>100_bases
ATTATGCGAATAATAGACCGCACGTGCAATAATCAACGACAGAATCATCGGCAATTTCGCTCATTATTTCATAAGCTTCT
GACATTCAGATGGGGTGGAC

Downstream 100 bases:

>100_bases
GAGCCGGCCGGCATTGTTCTTGAGGAAATCATGAAAGTCATCGTTCTGGGGGCCGGCATCGTCGGCGTCACATCCGCCTA
TCAGCTGGCCAAATCAGGTC

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MDSDILGSRERTVTITQGATGYLTIDLAALGRNYLKLASMLAPVRAGAVVKADAYGLGAERVAQTLYDQGCRHFFVAQFV
EAVRLRPALAEDAQIFVLNGLQPGNEVACAESGIIPVLNSLAQWRQWSATARLLTRSLPAVLQFDTGMSRLGFPWEERAE
LAAVIGDGANVEILFIMSHLACADELDSGQNGDQLAEMARIADEFPGFDISFANSGGVFLGDAYHGVLARPGIALYGGAP
NAGGTNPMEPVVRLDVAVVQTRTVPSGARIGYGGAHVTRRETRLATIAAGYADGLPRSLGDCGAVYHKGIRLPIVGRVSM
DSATVDITALPEGALRLGSLVEVLGPNQTLEDVAGAAGTISYEILTGLGDRYDRQYC

Sequences:

>Translated_377_residues
MDSDILGSRERTVTITQGATGYLTIDLAALGRNYLKLASMLAPVRAGAVVKADAYGLGAERVAQTLYDQGCRHFFVAQFV
EAVRLRPALAEDAQIFVLNGLQPGNEVACAESGIIPVLNSLAQWRQWSATARLLTRSLPAVLQFDTGMSRLGFPWEERAE
LAAVIGDGANVEILFIMSHLACADELDSGQNGDQLAEMARIADEFPGFDISFANSGGVFLGDAYHGVLARPGIALYGGAP
NAGGTNPMEPVVRLDVAVVQTRTVPSGARIGYGGAHVTRRETRLATIAAGYADGLPRSLGDCGAVYHKGIRLPIVGRVSM
DSATVDITALPEGALRLGSLVEVLGPNQTLEDVAGAAGTISYEILTGLGDRYDRQYC
>Mature_377_residues
MDSDILGSRERTVTITQGATGYLTIDLAALGRNYLKLASMLAPVRAGAVVKADAYGLGAERVAQTLYDQGCRHFFVAQFV
EAVRLRPALAEDAQIFVLNGLQPGNEVACAESGIIPVLNSLAQWRQWSATARLLTRSLPAVLQFDTGMSRLGFPWEERAE
LAAVIGDGANVEILFIMSHLACADELDSGQNGDQLAEMARIADEFPGFDISFANSGGVFLGDAYHGVLARPGIALYGGAP
NAGGTNPMEPVVRLDVAVVQTRTVPSGARIGYGGAHVTRRETRLATIAAGYADGLPRSLGDCGAVYHKGIRLPIVGRVSM
DSATVDITALPEGALRLGSLVEVLGPNQTLEDVAGAAGTISYEILTGLGDRYDRQYC

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=354, Percent_Identity=31.638418079096, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1790487, Length=369, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 39838; Mature: 39838

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSDILGSRERTVTITQGATGYLTIDLAALGRNYLKLASMLAPVRAGAVVKADAYGLGAE
CCCCCCCCCCCEEEEECCCCEEEEEEHHHHCHHHHHHHHHHHHHHCCCEEEECCCCCCHH
RVAQTLYDQGCRHFFVAQFVEAVRLRPALAEDAQIFVLNGLQPGNEVACAESGIIPVLNS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEECCCCCHHHHHH
LAQWRQWSATARLLTRSLPAVLQFDTGMSRLGFPWEERAELAAVIGDGANVEILFIMSHL
HHHHHHHHHHHHHHHHHCCCEEEECCCCHHCCCCHHHHHHEEEEECCCCCEEEEEEEHHH
ACADELDSGQNGDQLAEMARIADEFPGFDISFANSGGVFLGDAYHGVLARPGIALYGGAP
HHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCHHHCCCCEEEECCCC
NAGGTNPMEPVVRLDVAVVQTRTVPSGARIGYGGAHVTRRETRLATIAAGYADGLPRSLG
CCCCCCCCCHHHHHHHHEEEEECCCCCCCCCCCCCCEEHHHHHHHHEECHHHCCCCHHHH
DCGAVYHKGIRLPIVGRVSMDSATVDITALPEGALRLGSLVEVLGPNQTLEDVAGAAGTI
HHHHHHHCCCCEEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHCCCCCEE
SYEILTGLGDRYDRQYC
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDSDILGSRERTVTITQGATGYLTIDLAALGRNYLKLASMLAPVRAGAVVKADAYGLGAE
CCCCCCCCCCCEEEEECCCCEEEEEEHHHHCHHHHHHHHHHHHHHCCCEEEECCCCCCHH
RVAQTLYDQGCRHFFVAQFVEAVRLRPALAEDAQIFVLNGLQPGNEVACAESGIIPVLNS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEECCCCCHHHHHH
LAQWRQWSATARLLTRSLPAVLQFDTGMSRLGFPWEERAELAAVIGDGANVEILFIMSHL
HHHHHHHHHHHHHHHHHCCCEEEECCCCHHCCCCHHHHHHEEEEECCCCCEEEEEEEHHH
ACADELDSGQNGDQLAEMARIADEFPGFDISFANSGGVFLGDAYHGVLARPGIALYGGAP
HHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCHHHCCCCEEEECCCC
NAGGTNPMEPVVRLDVAVVQTRTVPSGARIGYGGAHVTRRETRLATIAAGYADGLPRSLG
CCCCCCCCCHHHHHHHHEEEEECCCCCCCCCCCCCCEEHHHHHHHHEECHHHCCCCHHHH
DCGAVYHKGIRLPIVGRVSMDSATVDITALPEGALRLGSLVEVLGPNQTLEDVAGAAGTI
HHHHHHHCCCCEEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHCCCCCEE
SYEILTGLGDRYDRQYC
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10792736 [H]