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The map label for this gene is radA [H]

Identifier: 190571640

GI number: 190571640

Start: 1340930

End: 1342282

Strand: Direct

Name: radA [H]

Synonym: WPa_1265

Alternate gene names: 190571640

Gene position: 1340930-1342282 (Clockwise)

Preceding gene: 190571639

Following gene: 190571643

Centisome position: 90.45

GC content: 36.81

Gene sequence:

>1353_bases
TTGGCTTTTATGAAAACTGCATACGTATGTCAATTCTGCGGTCATAGCACTGGAAGGTGGGTAGGAAGGTGTATTGCATG
TGACAGTTGGGATACGGTTGTCGAAGAAATAGTACTAAAAACAGAAACGAGAAGTACATCTGCACCAATTTCAATAAAAG
CGTTATCAGATAGTGAAATTATTACCCCCGATCGTTTTTTAACAGGAATAGAAGAGTTAGATAGAGTTTTTGGTGGAGGT
ATCGTTCAAGGTGCTAGCATTTTAATTGGTGGTGAACCTGGAATTGGCAAATCTACTCTTTTGCTTAGGATTGCAGCTAA
TGTTGTACAACTTTCCTCTTTTGAATGTCTTTATGTATCTGGCGAGGAATCTTTAGAGCAAGTGAGCCTCAGAGCAAAGC
GTCTTAAGATAAATGAACCTAAAATTAAGCTTTTATCTACAGTATCTTTAACTGATGTAGTAGCAACAATAAGGAAAAAT
AAAAGTATTAAGTTCTTGATAATTGATTCTATACAAACTATGTATGATAGCAAAGTCACATCAGCACCAGGAACTGTAAC
TCAAGTCCGTACCTGCGCTCATGAATTGACTGTTCTTGCAAAGCAATATAGTGTTTCTCTCTTAATAGTTGGCCATATAA
CGAAAGATGGACAAATAGCAGGACCTAAAACCCTGGAGCATATGGTAGATACAGTACTATATTTTGAGGGTGAAAATAGT
AATCAATATCGCATTTTACGCACTATTAAAAATAGATTTGGCCCTGCAAATGAAATTGGTGTTTTTGAGATGTCTGAAGC
AGGACTCATGCCTGTTGATAATCCATCATCATTATTCTTGACCGTACATGATAGAGAAGTAGTTGGCAGCGCCGTATTTG
CAGGAGTTGAAGGCTCAAGACCAATCTTAATGGAAGTGCAAGCATTAATAGCAAGGACTAATATGGCAACTCCAAGAAGA
GCAGTAGTTGGTTGGGATATTAATAGATTAGCTATGATAATCGCGGTTCTAAGTGCTCGTTGTAAAATGTTTTTGCATGA
TAAAGAAGTATACCTAAACATTGCGGGAGGACTCAAAATACAGGAACCATCAGCTGATCTTGCTGTTGCTGCTTCTCTTA
TTTCAAGTTTAGTAAATTTACCGCTACCAACTTCTTCAATAATCTGCGGTGAGGTTGCACTTTCAGGAGAAATTAGAAAT
GTTTCGCATGCTGATCTCAGACTAAGGGAAGCACAAAAGTTAGGATTTAAAAAAGCGATTATGCCTAAAAATGGCAATTA
TTCTTCTCATGATATTGAGATAATTAAGCTTGGTCATATAAAAGAGTTAAAAGAGATCTTCAATCACAATTAA

Upstream 100 bases:

>100_bases
AATATTGCAAATAAAACCACAAGATAAAAAGTGTGATTGGTAATTTTCTCTATTTAAATTTATTTCACTTATTTAGCTAA
TTTTTATTAGAATTATAATT

Downstream 100 bases:

>100_bases
ATATTATCTGCATGAACATTTTGCTTGTGAGAAGGATGTCATCCCAGCTTCCATAATGTCATTCAAGTAGGTGACACTGG
CTTCTTTTTTTACTGTTTTT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAFMKTAYVCQFCGHSTGRWVGRCIACDSWDTVVEEIVLKTETRSTSAPISIKALSDSEIITPDRFLTGIEELDRVFGGG
IVQGASILIGGEPGIGKSTLLLRIAANVVQLSSFECLYVSGEESLEQVSLRAKRLKINEPKIKLLSTVSLTDVVATIRKN
KSIKFLIIDSIQTMYDSKVTSAPGTVTQVRTCAHELTVLAKQYSVSLLIVGHITKDGQIAGPKTLEHMVDTVLYFEGENS
NQYRILRTIKNRFGPANEIGVFEMSEAGLMPVDNPSSLFLTVHDREVVGSAVFAGVEGSRPILMEVQALIARTNMATPRR
AVVGWDINRLAMIIAVLSARCKMFLHDKEVYLNIAGGLKIQEPSADLAVAASLISSLVNLPLPTSSIICGEVALSGEIRN
VSHADLRLREAQKLGFKKAIMPKNGNYSSHDIEIIKLGHIKELKEIFNHN

Sequences:

>Translated_450_residues
MAFMKTAYVCQFCGHSTGRWVGRCIACDSWDTVVEEIVLKTETRSTSAPISIKALSDSEIITPDRFLTGIEELDRVFGGG
IVQGASILIGGEPGIGKSTLLLRIAANVVQLSSFECLYVSGEESLEQVSLRAKRLKINEPKIKLLSTVSLTDVVATIRKN
KSIKFLIIDSIQTMYDSKVTSAPGTVTQVRTCAHELTVLAKQYSVSLLIVGHITKDGQIAGPKTLEHMVDTVLYFEGENS
NQYRILRTIKNRFGPANEIGVFEMSEAGLMPVDNPSSLFLTVHDREVVGSAVFAGVEGSRPILMEVQALIARTNMATPRR
AVVGWDINRLAMIIAVLSARCKMFLHDKEVYLNIAGGLKIQEPSADLAVAASLISSLVNLPLPTSSIICGEVALSGEIRN
VSHADLRLREAQKLGFKKAIMPKNGNYSSHDIEIIKLGHIKELKEIFNHN
>Mature_449_residues
AFMKTAYVCQFCGHSTGRWVGRCIACDSWDTVVEEIVLKTETRSTSAPISIKALSDSEIITPDRFLTGIEELDRVFGGGI
VQGASILIGGEPGIGKSTLLLRIAANVVQLSSFECLYVSGEESLEQVSLRAKRLKINEPKIKLLSTVSLTDVVATIRKNK
SIKFLIIDSIQTMYDSKVTSAPGTVTQVRTCAHELTVLAKQYSVSLLIVGHITKDGQIAGPKTLEHMVDTVLYFEGENSN
QYRILRTIKNRFGPANEIGVFEMSEAGLMPVDNPSSLFLTVHDREVVGSAVFAGVEGSRPILMEVQALIARTNMATPRRA
VVGWDINRLAMIIAVLSARCKMFLHDKEVYLNIAGGLKIQEPSADLAVAASLISSLVNLPLPTSSIICGEVALSGEIRNV
SHADLRLREAQKLGFKKAIMPKNGNYSSHDIEIIKLGHIKELKEIFNHN

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=454, Percent_Identity=42.7312775330396, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR020588
- InterPro:   IPR004504
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC [H]

EC number: NA

Molecular weight: Translated: 49151; Mature: 49020

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFMKTAYVCQFCGHSTGRWVGRCIACDSWDTVVEEIVLKTETRSTSAPISIKALSDSEI
CCCHHHHHHHHHHCCCCCCHHEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
ITPDRFLTGIEELDRVFGGGIVQGASILIGGEPGIGKSTLLLRIAANVVQLSSFECLYVS
CCHHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
GEESLEQVSLRAKRLKINEPKIKLLSTVSLTDVVATIRKNKSIKFLIIDSIQTMYDSKVT
CHHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCC
SAPGTVTQVRTCAHELTVLAKQYSVSLLIVGHITKDGQIAGPKTLEHMVDTVLYFEGENS
CCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCHHHHHHHHHEEEEEECCCC
NQYRILRTIKNRFGPANEIGVFEMSEAGLMPVDNPSSLFLTVHDREVVGSAVFAGVEGSR
CCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECHHHHHHHHEECCCCCC
PILMEVQALIARTNMATPRRAVVGWDINRLAMIIAVLSARCKMFLHDKEVYLNIAGGLKI
CHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHEEEECCEEEEEEECCEEE
QEPSADLAVAASLISSLVNLPLPTSSIICGEVALSGEIRNVSHADLRLREAQKLGFKKAI
ECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
MPKNGNYSSHDIEIIKLGHIKELKEIFNHN
CCCCCCCCCCCEEEEEECCHHHHHHHHCCC
>Mature Secondary Structure 
AFMKTAYVCQFCGHSTGRWVGRCIACDSWDTVVEEIVLKTETRSTSAPISIKALSDSEI
CCHHHHHHHHHHCCCCCCHHEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
ITPDRFLTGIEELDRVFGGGIVQGASILIGGEPGIGKSTLLLRIAANVVQLSSFECLYVS
CCHHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
GEESLEQVSLRAKRLKINEPKIKLLSTVSLTDVVATIRKNKSIKFLIIDSIQTMYDSKVT
CHHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCC
SAPGTVTQVRTCAHELTVLAKQYSVSLLIVGHITKDGQIAGPKTLEHMVDTVLYFEGENS
CCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCHHHHHHHHHEEEEEECCCC
NQYRILRTIKNRFGPANEIGVFEMSEAGLMPVDNPSSLFLTVHDREVVGSAVFAGVEGSR
CCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECHHHHHHHHEECCCCCC
PILMEVQALIARTNMATPRRAVVGWDINRLAMIIAVLSARCKMFLHDKEVYLNIAGGLKI
CHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHEEEECCEEEEEEECCEEE
QEPSADLAVAASLISSLVNLPLPTSSIICGEVALSGEIRNVSHADLRLREAQKLGFKKAI
ECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
MPKNGNYSSHDIEIIKLGHIKELKEIFNHN
CCCCCCCCCCCEEEEEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA