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The map label for this gene is shiA [H]

Identifier: 190571639

GI number: 190571639

Start: 1339601

End: 1340872

Strand: Direct

Name: shiA [H]

Synonym: WPa_1264

Alternate gene names: 190571639

Gene position: 1339601-1340872 (Clockwise)

Preceding gene: 190571638

Following gene: 190571640

Centisome position: 90.36

GC content: 34.28

Gene sequence:

>1272_bases
GTGTACAATCAACAAACAAGAGTATTGATTGCAACAATACTCTGTAGAATCGCAATATGGTATGACCATATGCTCTTTAT
TGATCTGGTTAATATAATTAGTAGAGAATTTTGTTATGCAAAAGATATCTACTACAGCATATTGCAATTGTTTGGAATTG
CAGGAATGGGTGCAATGGTAAGACCACTTGGTGCATCTGTATTTGGTCACATTGGTGATAGGTATGGAAGGAGAATGGCG
TTAACGATTGCTATCTTGCTAATATCAATTCCTTCTAGTCTCGTTGCGTTTATTCCAAGTTATAGTAAAATAGGAATAAC
CTCTACTATGTTGCTTCTTACAATCCATCTGATACAAGGGGTTGCACTTGGCGCTGAGCAAGGAGGTAGCTCTGTTTATC
TCATAGAGCATTTACCTAATAAGAAAAAACTAGGAATGTTTTTTGGAATAATAAGTTTTGGTCGCTCTATTGGCATATTG
CTCTCTGTGGTTGCAGTGATCATCTGTAAAAAAACCACTGATTTTAACGCTTGGGGTTGGAGGCTGCCGTTTATTTTTTC
AGCCATTTTAGGATTAATAAGCGCATATAGTATATATACACTAGGAGAAACTCCAGTGTATGAAAAAAATCGAGAACAAA
GAAATTTACCTAATTTACCAATAATAGAGCTTACAAAACGCTATAAAAGAGCCCTGATACTTGCCATTTTAATATCTGTA
CCTGTTAATGTTGCTGTTGGATTTACCATATTTCTTAGAACAATTGCAAAAGAAATAGTGTCTGTTGAAACTTATGTAAC
AACGTATGTCAACGAAGTTGTACTCATTATAACCAGTATATTAATGCCGATATCTTCAATAATGCTTGGAATTTTAGCTG
ATAAAACAGGAAGAGAACGCACTGCAATCTTATTTATAGTGATAACAATGGTATTATGTTGTCCTATGTTATCTATTGCA
TATTACTATAAAAGCTATCTTATAATCATGTTGAGTGTTATGGCTCTTTCTATAATCGAGAGGGGTATTAATCCTATAGG
GATAGTTACTGCTGAGCTTTTTCCTACTAATGTTAGATTTAGTGGTGTGAGTTTATCACGTAACATTTCTTATGCCCTGC
ATGGTGGATTTACTCCTATGATATGCACTGGGTTTACTATAATGTTTCCTAAAATAGACTTTGCTGCTGGGCTTTATGTA
ATTTTTTGCTTATTAGTCAGCTTAGTAGCAATATTGCAAATAAAACCACAAGATAAAAAGTGTGATTGGTAA

Upstream 100 bases:

>100_bases
CCAATTTTAATAATATTTACATATAAGCCATACGCTTGAGTTGCAAAGTTCTAAAAAGCAGCCAAAATAAAAATAAGTAG
ACATTTACAAATTAAACTAA

Downstream 100 bases:

>100_bases
TTTTCTCTATTTAAATTTATTTCACTTATTTAGCTAATTTTTATTAGAATTATAATTTTGGCTTTTATGAAAACTGCATA
CGTATGTCAATTCTGCGGTC

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MYNQQTRVLIATILCRIAIWYDHMLFIDLVNIISREFCYAKDIYYSILQLFGIAGMGAMVRPLGASVFGHIGDRYGRRMA
LTIAILLISIPSSLVAFIPSYSKIGITSTMLLLTIHLIQGVALGAEQGGSSVYLIEHLPNKKKLGMFFGIISFGRSIGIL
LSVVAVIICKKTTDFNAWGWRLPFIFSAILGLISAYSIYTLGETPVYEKNREQRNLPNLPIIELTKRYKRALILAILISV
PVNVAVGFTIFLRTIAKEIVSVETYVTTYVNEVVLIITSILMPISSIMLGILADKTGRERTAILFIVITMVLCCPMLSIA
YYYKSYLIIMLSVMALSIIERGINPIGIVTAELFPTNVRFSGVSLSRNISYALHGGFTPMICTGFTIMFPKIDFAAGLYV
IFCLLVSLVAILQIKPQDKKCDW

Sequences:

>Translated_423_residues
MYNQQTRVLIATILCRIAIWYDHMLFIDLVNIISREFCYAKDIYYSILQLFGIAGMGAMVRPLGASVFGHIGDRYGRRMA
LTIAILLISIPSSLVAFIPSYSKIGITSTMLLLTIHLIQGVALGAEQGGSSVYLIEHLPNKKKLGMFFGIISFGRSIGIL
LSVVAVIICKKTTDFNAWGWRLPFIFSAILGLISAYSIYTLGETPVYEKNREQRNLPNLPIIELTKRYKRALILAILISV
PVNVAVGFTIFLRTIAKEIVSVETYVTTYVNEVVLIITSILMPISSIMLGILADKTGRERTAILFIVITMVLCCPMLSIA
YYYKSYLIIMLSVMALSIIERGINPIGIVTAELFPTNVRFSGVSLSRNISYALHGGFTPMICTGFTIMFPKIDFAAGLYV
IFCLLVSLVAILQIKPQDKKCDW
>Mature_423_residues
MYNQQTRVLIATILCRIAIWYDHMLFIDLVNIISREFCYAKDIYYSILQLFGIAGMGAMVRPLGASVFGHIGDRYGRRMA
LTIAILLISIPSSLVAFIPSYSKIGITSTMLLLTIHLIQGVALGAEQGGSSVYLIEHLPNKKKLGMFFGIISFGRSIGIL
LSVVAVIICKKTTDFNAWGWRLPFIFSAILGLISAYSIYTLGETPVYEKNREQRNLPNLPIIELTKRYKRALILAILISV
PVNVAVGFTIFLRTIAKEIVSVETYVTTYVNEVVLIITSILMPISSIMLGILADKTGRERTAILFIVITMVLCCPMLSIA
YYYKSYLIIMLSVMALSIIERGINPIGIVTAELFPTNVRFSGVSLSRNISYALHGGFTPMICTGFTIMFPKIDFAAGLYV
IFCLLVSLVAILQIKPQDKKCDW

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=442, Percent_Identity=26.2443438914027, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1790550, Length=389, Percent_Identity=26.2210796915167, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789941, Length=398, Percent_Identity=26.1306532663317, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788942, Length=363, Percent_Identity=26.4462809917355, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47038; Mature: 47038

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNQQTRVLIATILCRIAIWYDHMLFIDLVNIISREFCYAKDIYYSILQLFGIAGMGAMV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RPLGASVFGHIGDRYGRRMALTIAILLISIPSSLVAFIPSYSKIGITSTMLLLTIHLIQG
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VALGAEQGGSSVYLIEHLPNKKKLGMFFGIISFGRSIGILLSVVAVIICKKTTDFNAWGW
HHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RLPFIFSAILGLISAYSIYTLGETPVYEKNREQRNLPNLPIIELTKRYKRALILAILISV
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
PVNVAVGFTIFLRTIAKEIVSVETYVTTYVNEVVLIITSILMPISSIMLGILADKTGRER
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TAILFIVITMVLCCPMLSIAYYYKSYLIIMLSVMALSIIERGINPIGIVTAELFPTNVRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEE
SGVSLSRNISYALHGGFTPMICTGFTIMFPKIDFAAGLYVIFCLLVSLVAILQIKPQDKK
ECEEECCCCEEEEECCCCHHHHCCEEHEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
CDW
CCC
>Mature Secondary Structure
MYNQQTRVLIATILCRIAIWYDHMLFIDLVNIISREFCYAKDIYYSILQLFGIAGMGAMV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RPLGASVFGHIGDRYGRRMALTIAILLISIPSSLVAFIPSYSKIGITSTMLLLTIHLIQG
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VALGAEQGGSSVYLIEHLPNKKKLGMFFGIISFGRSIGILLSVVAVIICKKTTDFNAWGW
HHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RLPFIFSAILGLISAYSIYTLGETPVYEKNREQRNLPNLPIIELTKRYKRALILAILISV
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
PVNVAVGFTIFLRTIAKEIVSVETYVTTYVNEVVLIITSILMPISSIMLGILADKTGRER
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TAILFIVITMVLCCPMLSIAYYYKSYLIIMLSVMALSIIERGINPIGIVTAELFPTNVRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEE
SGVSLSRNISYALHGGFTPMICTGFTIMFPKIDFAAGLYVIFCLLVSLVAILQIKPQDKK
ECEEECCCCEEEEECCCCHHHHCCEEHEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
CDW
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]