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The map label for this gene is greA [H]

Identifier: 190571574

GI number: 190571574

Start: 1269000

End: 1269494

Strand: Direct

Name: greA [H]

Synonym: WPa_1194

Alternate gene names: 190571574

Gene position: 1269000-1269494 (Clockwise)

Preceding gene: 190571573

Following gene: 190571575

Centisome position: 85.6

GC content: 35.96

Gene sequence:

>495_bases
ATGACTTCATCCATTGTTAACAAGTTTCCTATTACAAGGGAAGGTTTTGAGAGTATGCAAGTCGAACTTGAGAAATTAAA
GGAAGAAAAACCTTCTATCATACAGGCTATTTCTGATGCTCGTGATCAAGGCGATTTATCAGAAAATGCAGAATATCATG
CAGCACGGGAGAGATTAAGTTTTATTGAAGGCCGTATAATTGAGGTAGAAAGTAAACTTTCACATGCAGAAGTGATAGAA
GTAAAAAATTTGTCTGGTGATACAGTAATGTTTGGTGCAACTGTTACGGTAAGCATGCTCGGTGATGATAACAGTGAAAT
TGAATATGTTTATAAGATTGTAGGTGGATATGAAGCTGATGCTTCAAAGCAGTTGATCTCTACGGATTCACCACTCGGTA
GTGCTTTAATTGGCAAAAAAGTTGGTGAGTATGTGGAAGTGATAGTGCCAAATGGAGAGAAATTGTATAAAATAGTTAAG
ATTGAGTTTAAGTAA

Upstream 100 bases:

>100_bases
AAAAAAGTTGTCGGGTGACATGAATGATGATATAAAAGATGATATTCTTGGTATTGTGAGTGATTTTGTCACTCAATTTA
ATTGCGTTTAGGCGGTGATT

Downstream 100 bases:

>100_bases
ATCATGGTACAAGTAACTTATCCTTCAATTAGTCTACCTGGTATTTCTTCTGTGGAAGATGTATTGAGGGATGCTAGTTC
TGGTAAATTATTCATTTTAG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTSSIVNKFPITREGFESMQVELEKLKEEKPSIIQAISDARDQGDLSENAEYHAARERLSFIEGRIIEVESKLSHAEVIE
VKNLSGDTVMFGATVTVSMLGDDNSEIEYVYKIVGGYEADASKQLISTDSPLGSALIGKKVGEYVEVIVPNGEKLYKIVK
IEFK

Sequences:

>Translated_164_residues
MTSSIVNKFPITREGFESMQVELEKLKEEKPSIIQAISDARDQGDLSENAEYHAARERLSFIEGRIIEVESKLSHAEVIE
VKNLSGDTVMFGATVTVSMLGDDNSEIEYVYKIVGGYEADASKQLISTDSPLGSALIGKKVGEYVEVIVPNGEKLYKIVK
IEFK
>Mature_163_residues
TSSIVNKFPITREGFESMQVELEKLKEEKPSIIQAISDARDQGDLSENAEYHAARERLSFIEGRIIEVESKLSHAEVIEV
KNLSGDTVMFGATVTVSMLGDDNSEIEYVYKIVGGYEADASKQLISTDSPLGSALIGKKVGEYVEVIVPNGEKLYKIVKI
EFK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=47.1337579617834, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=32.4675324675325, Blast_Score=85, Evalue=3e-18,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18149; Mature: 18018

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSIVNKFPITREGFESMQVELEKLKEEKPSIIQAISDARDQGDLSENAEYHAARERLS
CCCCHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
FIEGRIIEVESKLSHAEVIEVKNLSGDTVMFGATVTVSMLGDDNSEIEYVYKIVGGYEAD
HHCCEEEEECCCCCHHHEEEEECCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHCCCCCC
ASKQLISTDSPLGSALIGKKVGEYVEVIVPNGEKLYKIVKIEFK
CCHHHHCCCCCCCHHHHHHHHHCEEEEEECCCCEEEEEEEEEEC
>Mature Secondary Structure 
TSSIVNKFPITREGFESMQVELEKLKEEKPSIIQAISDARDQGDLSENAEYHAARERLS
CCCHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
FIEGRIIEVESKLSHAEVIEVKNLSGDTVMFGATVTVSMLGDDNSEIEYVYKIVGGYEAD
HHCCEEEEECCCCCHHHEEEEECCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHCCCCCC
ASKQLISTDSPLGSALIGKKVGEYVEVIVPNGEKLYKIVKIEFK
CCHHHHCCCCCCCHHHHHHHHHCEEEEEECCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA