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The map label for this gene is tig

Identifier: 190570981

GI number: 190570981

Start: 613208

End: 614551

Strand: Direct

Name: tig

Synonym: WPa_0569

Alternate gene names: 190570981

Gene position: 613208-614551 (Clockwise)

Preceding gene: 190570979

Following gene: 190570982

Centisome position: 41.36

GC content: 31.03

Gene sequence:

>1344_bases
ATGTCTAGTAATATACCTCAAAATGCAGTCGAAGCAGATACATTAAGTAATATATATACCTATAAGGAACTTAGTGTAGA
TAAACTAAAGTATGAGTATGAAATCACAGTTAGTAGTGATTATATAGAACAAAAGATAAACTCTAGATTACAAGAAATAG
CGAAGAATGCAAAATTTCCTGGATTTAGATCAGGAAAGATGCCTTACGATCTTGTTATTACAAATTATAAAAACGAAGCT
TTGGAATATGTGATAAACGATACGATTGACTATTGCTCAAGTGATTTAATGAAAAAAATCGAAGTTAAGTCTCACATCCA
TCCTAAGGTTGATATTATGTCATTACCAAACCTTGATAAAAAGGATAAAAAAGGTGACTTTCTGTACAAATTATCTTTTG
AATCGATGCCAAAAGTGCCAGTAATAGATCTTGATAAAATAAATTTGAAGAAAGTTAAGGCAAAAATCAAAGAGGGAGAC
ATAAAAGAATTTATTGATTCTATAAAAACAAAATTTCCTAACTTTGTCTCTGTTGATGATGCTTCTTATCAAGCAAAAAA
CGAGGATAAACTGATAATTGACTTTGAAGGGCGAATTAGAAATAAGCTCTTTCAAGGTGGAAGTGGCAAAAATTTTACTG
TTAATTTAGGATCTGGAACATTTATAAATGGTTTTGAAGATCAATTAATTGGCATGAAAAAAGGGGAAACAAAAAGCTTT
AAGTTGAGATTTCCTGAAGATTACCAGGTAATTTCCCTTGCGGGACAGGAGGCTGCTTTTTCTGTTCGAGTTAATGACAT
TCAGATTGCCAAAGATTTGCAAAGTGATAATGAAATAGCTAGGAGAATTGGTTTTGAAGATCATTCTTCGCTAATAGATC
ATGTAAAAGAAGTAATTGATAATCAGTGTAAAGAAATGGAAGATCTCTTAATTAAGAAAGAGCTATTTGATTGTTTGGAT
GCTAGTTATAGTTTTGATTTGCCTATAGAAGTAGTAAAACAGGAACAGCAGAGAGTGGAAAGAGAGTTGAATGCTAAAGA
TGATTCGTGTAAAGAAGCTGAAAGACGCGTAAAGCTTGCAATGCTATTCATGGAGTTCAGTAAAGAACATGAAATATCAT
TAACTCAAAGTGATATTATGAATGTTATTGTAAATCAATATGTCAGTAAAGATGTATCATTTGACAGGGTATTTAAACAT
TTTAAATCAGATGGACAATTTCAGGAATTAGTTAGAGGGCAAGCGCTTGAGTATAAAGTGACGGATTATATGATAGAAAA
AGCTAGTAAAGAAGAACAGATTGTTTCTGTGAAAGAATTAAAGGAATTATTTGATAATATTTGA

Upstream 100 bases:

>100_bases
TTTGTTTGTATAAGAACCTGTTCTTAAGGATTGTATTTAACTTAAATTCTGGTAAAATGGCTTTATTAAAACATAAATAA
AATATTGATAACAGGTTGTA

Downstream 100 bases:

>100_bases
TAGGAATAAGGTATGACTCTTATACCAATTGTAGTTGAACAAACTAGTCGCGGTGAGCGGGCTTATGACATATATTCAAG
GCTAGTAAAAGAAAGAATAA

Product: trigger factor, putative

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSSNIPQNAVEADTLSNIYTYKELSVDKLKYEYEITVSSDYIEQKINSRLQEIAKNAKFPGFRSGKMPYDLVITNYKNEA
LEYVINDTIDYCSSDLMKKIEVKSHIHPKVDIMSLPNLDKKDKKGDFLYKLSFESMPKVPVIDLDKINLKKVKAKIKEGD
IKEFIDSIKTKFPNFVSVDDASYQAKNEDKLIIDFEGRIRNKLFQGGSGKNFTVNLGSGTFINGFEDQLIGMKKGETKSF
KLRFPEDYQVISLAGQEAAFSVRVNDIQIAKDLQSDNEIARRIGFEDHSSLIDHVKEVIDNQCKEMEDLLIKKELFDCLD
ASYSFDLPIEVVKQEQQRVERELNAKDDSCKEAERRVKLAMLFMEFSKEHEISLTQSDIMNVIVNQYVSKDVSFDRVFKH
FKSDGQFQELVRGQALEYKVTDYMIEKASKEEQIVSVKELKELFDNI

Sequences:

>Translated_447_residues
MSSNIPQNAVEADTLSNIYTYKELSVDKLKYEYEITVSSDYIEQKINSRLQEIAKNAKFPGFRSGKMPYDLVITNYKNEA
LEYVINDTIDYCSSDLMKKIEVKSHIHPKVDIMSLPNLDKKDKKGDFLYKLSFESMPKVPVIDLDKINLKKVKAKIKEGD
IKEFIDSIKTKFPNFVSVDDASYQAKNEDKLIIDFEGRIRNKLFQGGSGKNFTVNLGSGTFINGFEDQLIGMKKGETKSF
KLRFPEDYQVISLAGQEAAFSVRVNDIQIAKDLQSDNEIARRIGFEDHSSLIDHVKEVIDNQCKEMEDLLIKKELFDCLD
ASYSFDLPIEVVKQEQQRVERELNAKDDSCKEAERRVKLAMLFMEFSKEHEISLTQSDIMNVIVNQYVSKDVSFDRVFKH
FKSDGQFQELVRGQALEYKVTDYMIEKASKEEQIVSVKELKELFDNI
>Mature_446_residues
SSNIPQNAVEADTLSNIYTYKELSVDKLKYEYEITVSSDYIEQKINSRLQEIAKNAKFPGFRSGKMPYDLVITNYKNEAL
EYVINDTIDYCSSDLMKKIEVKSHIHPKVDIMSLPNLDKKDKKGDFLYKLSFESMPKVPVIDLDKINLKKVKAKIKEGDI
KEFIDSIKTKFPNFVSVDDASYQAKNEDKLIIDFEGRIRNKLFQGGSGKNFTVNLGSGTFINGFEDQLIGMKKGETKSFK
LRFPEDYQVISLAGQEAAFSVRVNDIQIAKDLQSDNEIARRIGFEDHSSLIDHVKEVIDNQCKEMEDLLIKKELFDCLDA
SYSFDLPIEVVKQEQQRVERELNAKDDSCKEAERRVKLAMLFMEFSKEHEISLTQSDIMNVIVNQYVSKDVSFDRVFKHF
KSDGQFQELVRGQALEYKVTDYMIEKASKEEQIVSVKELKELFDNI

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=421, Percent_Identity=24.2280285035629, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_WOLPP (B3CLB0)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975339.1
- EnsemblBacteria:   EBWOLT00000000619
- GeneID:   6385120
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0569
- GeneTree:   EBGT00050000031434
- HOGENOM:   HBG311097
- OMA:   FEGRIRN
- ProtClustDB:   CLSK749502
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 51566; Mature: 51435

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNIPQNAVEADTLSNIYTYKELSVDKLKYEYEITVSSDYIEQKINSRLQEIAKNAKFP
CCCCCCCCHHHHHHHHHHHHHHHCCHHHEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCC
GFRSGKMPYDLVITNYKNEALEYVINDTIDYCSSDLMKKIEVKSHIHPKVDIMSLPNLDK
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
KDKKGDFLYKLSFESMPKVPVIDLDKINLKKVKAKIKEGDIKEFIDSIKTKFPNFVSVDD
CCCCCCEEEEEECCCCCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECC
ASYQAKNEDKLIIDFEGRIRNKLFQGGSGKNFTVNLGSGTFINGFEDQLIGMKKGETKSF
CCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCCEECCHHHHHCCCCCCCCCEE
KLRFPEDYQVISLAGQEAAFSVRVNDIQIAKDLQSDNEIARRIGFEDHSSLIDHVKEVID
EEECCCCCEEEEECCCCEEEEEEECHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHH
NQCKEMEDLLIKKELFDCLDASYSFDLPIEVVKQEQQRVERELNAKDDSCKEAERRVKLA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
MLFMEFSKEHEISLTQSDIMNVIVNQYVSKDVSFDRVFKHFKSDGQFQELVRGQALEYKV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCCCEEHH
TDYMIEKASKEEQIVSVKELKELFDNI
HHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSNIPQNAVEADTLSNIYTYKELSVDKLKYEYEITVSSDYIEQKINSRLQEIAKNAKFP
CCCCCCCHHHHHHHHHHHHHHHCCHHHEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCC
GFRSGKMPYDLVITNYKNEALEYVINDTIDYCSSDLMKKIEVKSHIHPKVDIMSLPNLDK
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
KDKKGDFLYKLSFESMPKVPVIDLDKINLKKVKAKIKEGDIKEFIDSIKTKFPNFVSVDD
CCCCCCEEEEEECCCCCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECC
ASYQAKNEDKLIIDFEGRIRNKLFQGGSGKNFTVNLGSGTFINGFEDQLIGMKKGETKSF
CCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCCEECCHHHHHCCCCCCCCCEE
KLRFPEDYQVISLAGQEAAFSVRVNDIQIAKDLQSDNEIARRIGFEDHSSLIDHVKEVID
EEECCCCCEEEEECCCCEEEEEEECHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHH
NQCKEMEDLLIKKELFDCLDASYSFDLPIEVVKQEQQRVERELNAKDDSCKEAERRVKLA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
MLFMEFSKEHEISLTQSDIMNVIVNQYVSKDVSFDRVFKHFKSDGQFQELVRGQALEYKV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCCCEEHH
TDYMIEKASKEEQIVSVKELKELFDNI
HHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA