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The map label for this gene is hemK [H]

Identifier: 190570979

GI number: 190570979

Start: 610547

End: 611398

Strand: Direct

Name: hemK [H]

Synonym: WPa_0565

Alternate gene names: 190570979

Gene position: 610547-611398 (Clockwise)

Preceding gene: 190570967

Following gene: 190570981

Centisome position: 41.18

GC content: 33.92

Gene sequence:

>852_bases
ATGAAAACAATCAGCGCTTTAATTAAAGAAGGGTCAAAGCTACTATCATCACATAGAATTGAATCACCACATTTGGATTG
TGAAATCATCATGCAGTACGTACTTGGAGTAGAAAGATCATTCACAATCATGAATCACACCAATCAGGTACCAAGAAACA
AAGAACTTCTATTCTGGAAATTAACAAAAAAAAGAGCAGAAAGATATCCAATATCGCAAATAATAGGTAATCGTGAATTC
TGGAGTAAAAATTTTATAGTTAACCAACATGTTCTAGATCCAAGACCAGATAGTGAGACAGTAATTTCAACAGTGTTAAA
ATATTACCAAAATAAGAAGCAAAAAATAAAAATTGCAGATTTTGGCACAGGCACAGGCTGTTTGTTGATCTCCGTACTTA
GTGAGTATGAATATGCTATTGGAGTTGGTTTTGAAAAAAGCCTGGAAGCATACAAAATTACCTACCAGAACACAAAGAAA
CATAATCTTCTCAACAGAGCTAAAATATTTCCAAATTCGTGGATAGAATGCAGAGATTTATTTGATCTCATAATAAGCAA
TCCTCCATATATTAAAAGAAGTAAGTTAAAAGATCTGCAAGCTGAAGTGCAAAAAGAGCCAAGAATTGCTCTTGATGGCG
GTATCGATGGATTGAGCTGTTACCTGAGTATTTTTCCAATATTGAAAAAGTGCCTTAAAAAAGATGGGTTTGCGATATTG
GAAATAGGCGAAGATCAAAGCAATATTGACAAAATAATACCCTTATATGGACTGGCTTTTCAGGAATACGTATACGACTT
GGCTGGAATGAAAAGATGCATTGTTGTAAAGCAAGATAAAGAACATGACTAG

Upstream 100 bases:

>100_bases
ATCCTACTCTTGACACTTAGATTTAATGCAGCTACCCTAAGTTCAAAATTCTATACTCAATTTTGCTGTTCTTGCATACA
AGGAACACGTTTTTAGAATA

Downstream 100 bases:

>100_bases
TAGAACGCTAGCTTTCTAAGAACCTGTTCATAATCTCAAGAAAGACATAAAGTAAGAAATAGAGTGTATAAATTAGGATA
TATTAGGAGTGTATACCCAA

Product: modification methylase, HemK family

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKTISALIKEGSKLLSSHRIESPHLDCEIIMQYVLGVERSFTIMNHTNQVPRNKELLFWKLTKKRAERYPISQIIGNREF
WSKNFIVNQHVLDPRPDSETVISTVLKYYQNKKQKIKIADFGTGTGCLLISVLSEYEYAIGVGFEKSLEAYKITYQNTKK
HNLLNRAKIFPNSWIECRDLFDLIISNPPYIKRSKLKDLQAEVQKEPRIALDGGIDGLSCYLSIFPILKKCLKKDGFAIL
EIGEDQSNIDKIIPLYGLAFQEYVYDLAGMKRCIVVKQDKEHD

Sequences:

>Translated_283_residues
MKTISALIKEGSKLLSSHRIESPHLDCEIIMQYVLGVERSFTIMNHTNQVPRNKELLFWKLTKKRAERYPISQIIGNREF
WSKNFIVNQHVLDPRPDSETVISTVLKYYQNKKQKIKIADFGTGTGCLLISVLSEYEYAIGVGFEKSLEAYKITYQNTKK
HNLLNRAKIFPNSWIECRDLFDLIISNPPYIKRSKLKDLQAEVQKEPRIALDGGIDGLSCYLSIFPILKKCLKKDGFAIL
EIGEDQSNIDKIIPLYGLAFQEYVYDLAGMKRCIVVKQDKEHD
>Mature_283_residues
MKTISALIKEGSKLLSSHRIESPHLDCEIIMQYVLGVERSFTIMNHTNQVPRNKELLFWKLTKKRAERYPISQIIGNREF
WSKNFIVNQHVLDPRPDSETVISTVLKYYQNKKQKIKIADFGTGTGCLLISVLSEYEYAIGVGFEKSLEAYKITYQNTKK
HNLLNRAKIFPNSWIECRDLFDLIISNPPYIKRSKLKDLQAEVQKEPRIALDGGIDGLSCYLSIFPILKKCLKKDGFAIL
EIGEDQSNIDKIIPLYGLAFQEYVYDLAGMKRCIVVKQDKEHD

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=233, Percent_Identity=25.7510729613734, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1787463, Length=230, Percent_Identity=29.1304347826087, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082085, Length=214, Percent_Identity=27.5700934579439, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24582226, Length=250, Percent_Identity=28.4, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32653; Mature: 32653

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00092 N6_MTASE ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTISALIKEGSKLLSSHRIESPHLDCEIIMQYVLGVERSFTIMNHTNQVPRNKELLFWK
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEE
LTKKRAERYPISQIIGNREFWSKNFIVNQHVLDPRPDSETVISTVLKYYQNKKQKIKIAD
EHHHHHHCCCHHHHHCCHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
FGTGTGCLLISVLSEYEYAIGVGFEKSLEAYKITYQNTKKHNLLNRAKIFPNSWIECRDL
CCCCHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHH
FDLIISNPPYIKRSKLKDLQAEVQKEPRIALDGGIDGLSCYLSIFPILKKCLKKDGFAIL
HHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEE
EIGEDQSNIDKIIPLYGLAFQEYVYDLAGMKRCIVVKQDKEHD
EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
>Mature Secondary Structure
MKTISALIKEGSKLLSSHRIESPHLDCEIIMQYVLGVERSFTIMNHTNQVPRNKELLFWK
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEE
LTKKRAERYPISQIIGNREFWSKNFIVNQHVLDPRPDSETVISTVLKYYQNKKQKIKIAD
EHHHHHHCCCHHHHHCCHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
FGTGTGCLLISVLSEYEYAIGVGFEKSLEAYKITYQNTKKHNLLNRAKIFPNSWIECRDL
CCCCHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHH
FDLIISNPPYIKRSKLKDLQAEVQKEPRIALDGGIDGLSCYLSIFPILKKCLKKDGFAIL
HHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEE
EIGEDQSNIDKIIPLYGLAFQEYVYDLAGMKRCIVVKQDKEHD
EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]