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The map label for this gene is dus [H]

Identifier: 190570967

GI number: 190570967

Start: 593212

End: 594162

Strand: Direct

Name: dus [H]

Synonym: WPa_0553

Alternate gene names: 190570967

Gene position: 593212-594162 (Clockwise)

Preceding gene: 190570966

Following gene: 190570979

Centisome position: 40.02

GC content: 38.91

Gene sequence:

>951_bases
ATGTCCCTACAAATAGGAAGTTTGACGATTGATTCTCCAGTTATTTTAGCGCCAATGTCTGGAGTGACTGACTATCCATT
TAGGAGTATTGTAAAGAAACTTGGTGCAAGTTTGCTAGTCTCAGAAATGATTGCAAGCAGAGCTATGATCATGCAAACTC
GCCAGAGCATGCAAAAAGCAAAAGTTGATGAACTAACCGCTGTACAACTTGCTGGTTGTGAGCCTGATGTTATGGCGGAA
GCTGCAAAGTTAAATGAGGGCATGGGTGCAAAAATCATAGATATAAATTTTGGTTGTCCAGTTAAAAAAGTTGTAAACGG
TTACGCAGGGTCAGCACTTATGCGTGATGAAAAAAAGGCTGCTGAGATAATAGAAGCTGTAGTCAAGGCAGTGAGTGTGC
CAGTTACAGTAAAAATGCGTACTGGATGGAATGACGAAAATCGTAATGCTCCGCGCTTGGCAAAAATTGCAGAAGATTTG
GGAGCAAAGATGATTACTGTACATGGAAGGACAAGGGCACAGCTTTATAATGGTCAAGCAGATTGGAAATTCGTTAGAAA
TGTTAAAGAGCAGGTAAAAATTCCAGTTATAGTAAATGGAGATATTAAGAGTTTGAATGATGTTCAAAGTGCACTTAAAG
AATCTGGGGCAGATGGAGTAATGATAGGTCGTGGTACTTACGGCAAACCTTGGCTGATTAATCAAGCAATAAACTTTTTG
AGTGGAAATAAAGTTTCTGAGCCAACAGCCTCAGAGAGATTAAGCATAATACTCAAGCATTATGACAGTATTCTTGAGTA
CTATGGAAGCGATACAGGAATAAAGATGGCCCGCAAACATATAGGATGGTACAGTAGCGGGCTTCAAAACTCATCTGAAT
TTCGTGTAAGAGTAAATAACATAATAGACAGCACAGAGGTGAAAGAAAATATAATCGATTTTTTTAGTTAA

Upstream 100 bases:

>100_bases
GAGCCTTCTCTTATAGAGAAAGTAAATTCATTTGTGCAAGATGTTATAATAGCAGCTTGTAATAGTGTTAAAAATGCATT
TTCTAGCGACTAGAAAGCCT

Downstream 100 bases:

>100_bases
AAACAGATTAAACATACTACCTCATTAGATATATTCAATTTGACTGATCATAGTGCGATGCCACCACACTCTCTTCTTCA
TCAGAATGTAATTGATAACT

Product: NifR3 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSLQIGSLTIDSPVILAPMSGVTDYPFRSIVKKLGASLLVSEMIASRAMIMQTRQSMQKAKVDELTAVQLAGCEPDVMAE
AAKLNEGMGAKIIDINFGCPVKKVVNGYAGSALMRDEKKAAEIIEAVVKAVSVPVTVKMRTGWNDENRNAPRLAKIAEDL
GAKMITVHGRTRAQLYNGQADWKFVRNVKEQVKIPVIVNGDIKSLNDVQSALKESGADGVMIGRGTYGKPWLINQAINFL
SGNKVSEPTASERLSIILKHYDSILEYYGSDTGIKMARKHIGWYSSGLQNSSEFRVRVNNIIDSTEVKENIIDFFS

Sequences:

>Translated_316_residues
MSLQIGSLTIDSPVILAPMSGVTDYPFRSIVKKLGASLLVSEMIASRAMIMQTRQSMQKAKVDELTAVQLAGCEPDVMAE
AAKLNEGMGAKIIDINFGCPVKKVVNGYAGSALMRDEKKAAEIIEAVVKAVSVPVTVKMRTGWNDENRNAPRLAKIAEDL
GAKMITVHGRTRAQLYNGQADWKFVRNVKEQVKIPVIVNGDIKSLNDVQSALKESGADGVMIGRGTYGKPWLINQAINFL
SGNKVSEPTASERLSIILKHYDSILEYYGSDTGIKMARKHIGWYSSGLQNSSEFRVRVNNIIDSTEVKENIIDFFS
>Mature_315_residues
SLQIGSLTIDSPVILAPMSGVTDYPFRSIVKKLGASLLVSEMIASRAMIMQTRQSMQKAKVDELTAVQLAGCEPDVMAEA
AKLNEGMGAKIIDINFGCPVKKVVNGYAGSALMRDEKKAAEIIEAVVKAVSVPVTVKMRTGWNDENRNAPRLAKIAEDLG
AKMITVHGRTRAQLYNGQADWKFVRNVKEQVKIPVIVNGDIKSLNDVQSALKESGADGVMIGRGTYGKPWLINQAINFLS
GNKVSEPTASERLSIILKHYDSILEYYGSDTGIKMARKHIGWYSSGLQNSSEFRVRVNNIIDSTEVKENIIDFFS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=281, Percent_Identity=32.7402135231317, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI239788462, Length=285, Percent_Identity=32.9824561403509, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=30.3964757709251, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=30.7017543859649, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI8923374, Length=252, Percent_Identity=26.984126984127, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=40.3785488958991, Blast_Score=257, Evalue=8e-70,
Organism=Escherichia coli, GI1788462, Length=285, Percent_Identity=30.1754385964912, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI145693211, Length=301, Percent_Identity=26.9102990033223, Blast_Score=104, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17543114, Length=291, Percent_Identity=31.6151202749141, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17507177, Length=284, Percent_Identity=28.8732394366197, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25144369, Length=211, Percent_Identity=27.4881516587678, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=306, Percent_Identity=27.1241830065359, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=28.695652173913, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=25.1101321585903, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24585320, Length=287, Percent_Identity=31.0104529616725, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24580595, Length=228, Percent_Identity=32.0175438596491, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19920448, Length=228, Percent_Identity=32.0175438596491, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921524, Length=314, Percent_Identity=26.4331210191083, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45549423, Length=233, Percent_Identity=28.3261802575107, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34612; Mature: 34481

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQIGSLTIDSPVILAPMSGVTDYPFRSIVKKLGASLLVSEMIASRAMIMQTRQSMQKA
CCEEECCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVDELTAVQLAGCEPDVMAEAAKLNEGMGAKIIDINFGCPVKKVVNGYAGSALMRDEKKA
HHHHHHEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCHHHHHHHHHH
AEIIEAVVKAVSVPVTVKMRTGWNDENRNAPRLAKIAEDLGAKMITVHGRTRAQLYNGQA
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCEEEEECCCC
DWKFVRNVKEQVKIPVIVNGDIKSLNDVQSALKESGADGVMIGRGTYGKPWLINQAINFL
HHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHH
SGNKVSEPTASERLSIILKHYDSILEYYGSDTGIKMARKHIGWYSSGLQNSSEFRVRVNN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHCCCCCCHHEEEEHH
IIDSTEVKENIIDFFS
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLQIGSLTIDSPVILAPMSGVTDYPFRSIVKKLGASLLVSEMIASRAMIMQTRQSMQKA
CEEECCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVDELTAVQLAGCEPDVMAEAAKLNEGMGAKIIDINFGCPVKKVVNGYAGSALMRDEKKA
HHHHHHEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCHHHHHHHHHH
AEIIEAVVKAVSVPVTVKMRTGWNDENRNAPRLAKIAEDLGAKMITVHGRTRAQLYNGQA
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCEEEEECCCC
DWKFVRNVKEQVKIPVIVNGDIKSLNDVQSALKESGADGVMIGRGTYGKPWLINQAINFL
HHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHH
SGNKVSEPTASERLSIILKHYDSILEYYGSDTGIKMARKHIGWYSSGLQNSSEFRVRVNN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHCCCCCCHHEEEEHH
IIDSTEVKENIIDFFS
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]