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The map label for this gene is mraW [H]

Identifier: 189184784

GI number: 189184784

Start: 1917684

End: 1918622

Strand: Reverse

Name: mraW [H]

Synonym: OTT_1877

Alternate gene names: 189184784

Gene position: 1918622-1917684 (Counterclockwise)

Preceding gene: 189184785

Following gene: 189184783

Centisome position: 95.5

GC content: 29.82

Gene sequence:

>939_bases
TTGAATAACATTCATACTCCAGTGCTAGCTACAGAAATGCTAAGTTACTTAGCTCCAGTAGACAATGAAACATATCTAGA
TTGCACATTTGGGGCTGGTGGATATAGCAAGCTAATTTTGTCAAATTGTAATTGTAAAGTTATTGCTTTTGACAGAGATC
CAGCTGTAATAAGCATTGCTAGTCAATTTTATCAACAATACCCAAATAGATTTACTTTTTTTAATGAAAATTTTGTTGAA
GCTAATAAGTATTTATCTAAATTAGATAAACTAAACGGTATAGTAATGGATTTAGGTGTTTCGTCAATGCAACTAGATGA
AGCTAATCGAGGTTTTTCATTTCGTTATGATGCTGAGCTTGATATGAGAATGAGTCAAAAAGGGTACAAGGCTTCAGAAT
TTGTTAATGAAGCATCTGAACATCAATTAGCTGATATTATTTACAAGTTTGGTGAAGAAAATAAAGCTAATAAAATTGCT
AAGCATATTGTGCTTGCCAGAAAAAAAAAACCAATTACAACTACTCTGCAATTAGCCAATATAATTAGAGAAGCCGTTGG
ATATAACAATTATTATAAAAAGAATAAAATTGATTCAGCTACAAAAACTTTTCAGGCTATTAGAATTTTTATCAATGATG
AATTATCTGCTATACAAAACTTCCTTAATCAAAGTTTGGAATTATTAGCTGTTAACGGCAGACTTATTGTAGTTAGTTTT
CATGCATTAGAAGATGCTATTATAAAAAAGTTTATGCATCAAAATGCAGTAAAGAAAGTTGCTCAATCAAAATATAGTAC
TAATAGGCAATCACCTTTACAAAATGGAGTATTATATTTATTAACTAAGAAAATAGCAGTACCAACTAGAACAGAGATTA
TCAATAATCCTAGATCTAGATCTGCAAGATTAAGGGCGGCATTAAAAATTAATGAATAG

Upstream 100 bases:

>100_bases
TAAGAAATTTTTGGACTAGTGGTAGTGAATAAGAATAAATTATATGCCATTTAAGTAGTTACTAATAGTTTAAAATAAGT
ATTGTAAGGAAACAGTTGGA

Downstream 100 bases:

>100_bases
TAAAATTATGTTGAATATTATAAATTGCGCCATTGTTATAATGATATTTATTGTTGGTTATTACCTATTTGCAATAAAGA
ATGATGTAAATAATTTAAAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNNIHTPVLATEMLSYLAPVDNETYLDCTFGAGGYSKLILSNCNCKVIAFDRDPAVISIASQFYQQYPNRFTFFNENFVE
ANKYLSKLDKLNGIVMDLGVSSMQLDEANRGFSFRYDAELDMRMSQKGYKASEFVNEASEHQLADIIYKFGEENKANKIA
KHIVLARKKKPITTTLQLANIIREAVGYNNYYKKNKIDSATKTFQAIRIFINDELSAIQNFLNQSLELLAVNGRLIVVSF
HALEDAIIKKFMHQNAVKKVAQSKYSTNRQSPLQNGVLYLLTKKIAVPTRTEIINNPRSRSARLRAALKINE

Sequences:

>Translated_312_residues
MNNIHTPVLATEMLSYLAPVDNETYLDCTFGAGGYSKLILSNCNCKVIAFDRDPAVISIASQFYQQYPNRFTFFNENFVE
ANKYLSKLDKLNGIVMDLGVSSMQLDEANRGFSFRYDAELDMRMSQKGYKASEFVNEASEHQLADIIYKFGEENKANKIA
KHIVLARKKKPITTTLQLANIIREAVGYNNYYKKNKIDSATKTFQAIRIFINDELSAIQNFLNQSLELLAVNGRLIVVSF
HALEDAIIKKFMHQNAVKKVAQSKYSTNRQSPLQNGVLYLLTKKIAVPTRTEIINNPRSRSARLRAALKINE
>Mature_312_residues
MNNIHTPVLATEMLSYLAPVDNETYLDCTFGAGGYSKLILSNCNCKVIAFDRDPAVISIASQFYQQYPNRFTFFNENFVE
ANKYLSKLDKLNGIVMDLGVSSMQLDEANRGFSFRYDAELDMRMSQKGYKASEFVNEASEHQLADIIYKFGEENKANKIA
KHIVLARKKKPITTTLQLANIIREAVGYNNYYKKNKIDSATKTFQAIRIFINDELSAIQNFLNQSLELLAVNGRLIVVSF
HALEDAIIKKFMHQNAVKKVAQSKYSTNRQSPLQNGVLYLLTKKIAVPTRTEIINNPRSRSARLRAALKINE

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=343, Percent_Identity=37.3177842565598, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI165377202, Length=193, Percent_Identity=40.4145077720207, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1786270, Length=313, Percent_Identity=38.0191693290735, Blast_Score=212, Evalue=3e-56,
Organism=Drosophila melanogaster, GI62472493, Length=333, Percent_Identity=38.7387387387387, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI28571637, Length=333, Percent_Identity=38.7387387387387, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35432; Mature: 35432

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIHTPVLATEMLSYLAPVDNETYLDCTFGAGGYSKLILSNCNCKVIAFDRDPAVISIA
CCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHEEECCCEEEEEECCCCCHHHHH
SQFYQQYPNRFTFFNENFVEANKYLSKLDKLNGIVMDLGVSSMQLDEANRGFSFRYDAEL
HHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHCCCCCCCEEEECCCC
DMRMSQKGYKASEFVNEASEHQLADIIYKFGEENKANKIAKHIVLARKKKPITTTLQLAN
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
IIREAVGYNNYYKKNKIDSATKTFQAIRIFINDELSAIQNFLNQSLELLAVNGRLIVVSF
HHHHHHCCCCCHHHCCCCHHHHHHHHHHEEECCHHHHHHHHHHCCEEEEEECCEEEEEEH
HALEDAIIKKFMHQNAVKKVAQSKYSTNRQSPLQNGVLYLLTKKIAVPTRTEIINNPRSR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEHHHCCCCHHHHHCCCCHH
SARLRAALKINE
HHHEEEEEEECC
>Mature Secondary Structure
MNNIHTPVLATEMLSYLAPVDNETYLDCTFGAGGYSKLILSNCNCKVIAFDRDPAVISIA
CCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHEEECCCEEEEEECCCCCHHHHH
SQFYQQYPNRFTFFNENFVEANKYLSKLDKLNGIVMDLGVSSMQLDEANRGFSFRYDAEL
HHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHCCCCCCCEEEECCCC
DMRMSQKGYKASEFVNEASEHQLADIIYKFGEENKANKIAKHIVLARKKKPITTTLQLAN
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
IIREAVGYNNYYKKNKIDSATKTFQAIRIFINDELSAIQNFLNQSLELLAVNGRLIVVSF
HHHHHHCCCCCHHHCCCCHHHHHHHHHHEEECCHHHHHHHHHHCCEEEEEECCEEEEEEH
HALEDAIIKKFMHQNAVKKVAQSKYSTNRQSPLQNGVLYLLTKKIAVPTRTEIINNPRSR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEHHHCCCCHHHHHCCCCHH
SARLRAALKINE
HHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA