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The map label for this gene is pbpA1 [H]

Identifier: 189184485

GI number: 189184485

Start: 1608509

End: 1610260

Strand: Reverse

Name: pbpA1 [H]

Synonym: OTT_1578

Alternate gene names: 189184485

Gene position: 1610260-1608509 (Counterclockwise)

Preceding gene: 189184486

Following gene: 189184483

Centisome position: 80.15

GC content: 31.62

Gene sequence:

>1752_bases
ATGTTGAATAACAAAGTACATCAAAACAGATTGATTAATCGCAGAGCTTTTCTATTATTGATAGGGAAATTAGGTCTATT
ATGTATGTTAGCTGGTAGACTTTTTCACTTGCAAATTTTTCAATCTAGTAAATATAAAATTTTATCAGATAAAAATAGGA
TTAATATTGTATTATTAATAGCTAGGAGAGGAAAAATTTTAGATTGTAATGGTAATGTTTTAGCATTTAATAAGCGTGCT
TATCAAATTAAGGCAAATAAGAAGACTTTAGCTCAAAATCAATATGTTGCTCTTGATTTACTATATGATATTTTAAATTT
TAGTAACGAAGAACGTGAACAAATTAACAAAAAAATACTTACTAGTAAAAATCTTTATGAAGTTCTTATTATGGATAATA
TTTCATGGGATAAGCTAGCTGCAATAGAAGAAAATTCATTAAATTTGCCAGGTATATATATTGATTGTATGGAATATAGA
ACCTATACTTATCCATTATCAACGTCACATATTTTAGGTTATACAGCATGGCCTAATGATGCTGAAAGGGTAAATTTAAA
TATAGAACACACTAGGATTCAAATTGGAAAAAGAGGTCTTGAAAAACATTTTGAGGAGGATCTTAGGGGAACTTGTGGCT
TTGAAAAGATTGAAGTGGATGCCAGAGGCCTAAAGGTTAGAGAGCTTGATACTATACCTAGTATTCCTGGTAAGGATATA
GCATTAAGCATTGATGTTAATTTGCAAAATAAACTTTATGAGATGTTTGATGATAGAGTAGGGGCTGCAATATTAACTGA
TGTAAATACTGGAAAGATTAAAGCTCTAGTTTCAGTTCCAGGTTATAATATTAATGCTTTTCCTAGAAGAATTAGTACAA
AGGACTGGAAAGTGCTTCTAGATAATCCTAATCTACCATTGGTTAGTCGTGCTTCACAATCGTTATATCCTCCTGGTTCA
ATATTTCAATTAGTAACCTTACTTGCAGCTTTAGAAGCAGGAGTTAATCCTAAGTTAACTGTATATTGTAATGGTAAAAG
CTTTATGAACGATACTAATTTTAGCTGTTGGAATCATCGTGGGCATGGTAATGTTGACATGCTTAGTGCAATTAAATACT
CGTGTAATAATTATATTTATCATCTTGCTAGTTTGATTGGCTATAATGCTATTTTCGCAATGGCTAGAAAGTTAGGATTT
TGGAGAAGGGCTGTAGTAAATCTTCCGAATGAACTAGTTGGAAATTTACCTACGCCAGAATGGAAAAAACGAAAAACAAA
ACAACGATGGATGTTAGGTGATACACTTAATGTGGCTATTGGGCAAGGATATATTACTACTACTCCTTTGCAGTTAGTAA
GAATGATTACTAGCATTGCTAATGGCAAGCAATTATTGCAGTTAAGTTTGACTTTAGATGAAGATGGTTATCATAAAAAT
TTGAATATTAATGCAGGTCATATTGACTTTATTCGTCAAGCTTTAAAGGCTGTTATTAATAATGAAAGTAGTGGTACTGC
TTATTCTTTGGATAGTGATAAAACTAAGCTAATGATTTCAGGTAAAACAGGTAGTGCTCAAGTAGGATCATCTTTGCAAA
GTCAAGCTGCTCATGGGTTATTTGTTGGATTTTCTACTACTAATAAGCCTGAATATGCTGTTGCAGTGATTTTAGAACAT
GGTGGACAAATCGGAATTAAATCTGCATCTGCAATTGCTAAAAGTATTTTGATCGAAGCGTCAACTTTATAA

Upstream 100 bases:

>100_bases
AATTATCAGATTCTAATTTTCAAAATTCTAACTAGCAGCTTGTGTTATTTTCCTTTAAGCAAAATTTTCCAATATATTTT
TCGTAATTTAACACTCAGTG

Downstream 100 bases:

>100_bases
ACACCAGATAAGGTTTGCAAGGTATAGTATTTCGTTATAATTTTTAATATATTACATTAAATACCACTTAATTTATAACT
GTTTTATAGTAGTTTGGCTA

Product: penicillin-binding protein PbpA1

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MLNNKVHQNRLINRRAFLLLIGKLGLLCMLAGRLFHLQIFQSSKYKILSDKNRINIVLLIARRGKILDCNGNVLAFNKRA
YQIKANKKTLAQNQYVALDLLYDILNFSNEEREQINKKILTSKNLYEVLIMDNISWDKLAAIEENSLNLPGIYIDCMEYR
TYTYPLSTSHILGYTAWPNDAERVNLNIEHTRIQIGKRGLEKHFEEDLRGTCGFEKIEVDARGLKVRELDTIPSIPGKDI
ALSIDVNLQNKLYEMFDDRVGAAILTDVNTGKIKALVSVPGYNINAFPRRISTKDWKVLLDNPNLPLVSRASQSLYPPGS
IFQLVTLLAALEAGVNPKLTVYCNGKSFMNDTNFSCWNHRGHGNVDMLSAIKYSCNNYIYHLASLIGYNAIFAMARKLGF
WRRAVVNLPNELVGNLPTPEWKKRKTKQRWMLGDTLNVAIGQGYITTTPLQLVRMITSIANGKQLLQLSLTLDEDGYHKN
LNINAGHIDFIRQALKAVINNESSGTAYSLDSDKTKLMISGKTGSAQVGSSLQSQAAHGLFVGFSTTNKPEYAVAVILEH
GGQIGIKSASAIAKSILIEASTL

Sequences:

>Translated_583_residues
MLNNKVHQNRLINRRAFLLLIGKLGLLCMLAGRLFHLQIFQSSKYKILSDKNRINIVLLIARRGKILDCNGNVLAFNKRA
YQIKANKKTLAQNQYVALDLLYDILNFSNEEREQINKKILTSKNLYEVLIMDNISWDKLAAIEENSLNLPGIYIDCMEYR
TYTYPLSTSHILGYTAWPNDAERVNLNIEHTRIQIGKRGLEKHFEEDLRGTCGFEKIEVDARGLKVRELDTIPSIPGKDI
ALSIDVNLQNKLYEMFDDRVGAAILTDVNTGKIKALVSVPGYNINAFPRRISTKDWKVLLDNPNLPLVSRASQSLYPPGS
IFQLVTLLAALEAGVNPKLTVYCNGKSFMNDTNFSCWNHRGHGNVDMLSAIKYSCNNYIYHLASLIGYNAIFAMARKLGF
WRRAVVNLPNELVGNLPTPEWKKRKTKQRWMLGDTLNVAIGQGYITTTPLQLVRMITSIANGKQLLQLSLTLDEDGYHKN
LNINAGHIDFIRQALKAVINNESSGTAYSLDSDKTKLMISGKTGSAQVGSSLQSQAAHGLFVGFSTTNKPEYAVAVILEH
GGQIGIKSASAIAKSILIEASTL
>Mature_583_residues
MLNNKVHQNRLINRRAFLLLIGKLGLLCMLAGRLFHLQIFQSSKYKILSDKNRINIVLLIARRGKILDCNGNVLAFNKRA
YQIKANKKTLAQNQYVALDLLYDILNFSNEEREQINKKILTSKNLYEVLIMDNISWDKLAAIEENSLNLPGIYIDCMEYR
TYTYPLSTSHILGYTAWPNDAERVNLNIEHTRIQIGKRGLEKHFEEDLRGTCGFEKIEVDARGLKVRELDTIPSIPGKDI
ALSIDVNLQNKLYEMFDDRVGAAILTDVNTGKIKALVSVPGYNINAFPRRISTKDWKVLLDNPNLPLVSRASQSLYPPGS
IFQLVTLLAALEAGVNPKLTVYCNGKSFMNDTNFSCWNHRGHGNVDMLSAIKYSCNNYIYHLASLIGYNAIFAMARKLGF
WRRAVVNLPNELVGNLPTPEWKKRKTKQRWMLGDTLNVAIGQGYITTTPLQLVRMITSIANGKQLLQLSLTLDEDGYHKN
LNINAGHIDFIRQALKAVINNESSGTAYSLDSDKTKLMISGKTGSAQVGSSLQSQAAHGLFVGFSTTNKPEYAVAVILEH
GGQIGIKSASAIAKSILIEASTL

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=587, Percent_Identity=30.664395229983, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1786272, Length=469, Percent_Identity=24.0938166311301, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 65192; Mature: 65192

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNNKVHQNRLINRRAFLLLIGKLGLLCMLAGRLFHLQIFQSSKYKILSDKNRINIVLLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCCEEEEEEE
ARRGKILDCNGNVLAFNKRAYQIKANKKTLAQNQYVALDLLYDILNFSNEEREQINKKIL
ECCCEEEECCCCEEEEECEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
TSKNLYEVLIMDNISWDKLAAIEENSLNLPGIYIDCMEYRTYTYPLSTSHILGYTAWPND
CCCCEEEEEEECCCCHHHEEEECCCCCCCCEEEEEEEECEEEEECCCCCCEEEEECCCCC
AERVNLNIEHTRIQIGKRGLEKHFEEDLRGTCGFEKIEVDARGLKVRELDTIPSIPGKDI
CCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCCCCCE
ALSIDVNLQNKLYEMFDDRVGAAILTDVNTGKIKALVSVPGYNINAFPRRISTKDWKVLL
EEEEEECHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEE
DNPNLPLVSRASQSLYPPGSIFQLVTLLAALEAGVNPKLTVYCNGKSFMNDTNFSCWNHR
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEECCC
GHGNVDMLSAIKYSCNNYIYHLASLIGYNAIFAMARKLGFWRRAVVNLPNELVGNLPTPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCH
WKKRKTKQRWMLGDTLNVAIGQGYITTTPLQLVRMITSIANGKQLLQLSLTLDEDGYHKN
HHHHHHHHHEECCCEEEEEECCCEEEECHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCE
LNINAGHIDFIRQALKAVINNESSGTAYSLDSDKTKLMISGKTGSAQVGSSLQSQAAHGL
ECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCHHHCCHHHHHCCCEE
FVGFSTTNKPEYAVAVILEHGGQIGIKSASAIAKSILIEASTL
EEEECCCCCCCEEEEEEEECCCCEEECHHHHHHHHHHEEECCC
>Mature Secondary Structure
MLNNKVHQNRLINRRAFLLLIGKLGLLCMLAGRLFHLQIFQSSKYKILSDKNRINIVLLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCCEEEEEEE
ARRGKILDCNGNVLAFNKRAYQIKANKKTLAQNQYVALDLLYDILNFSNEEREQINKKIL
ECCCEEEECCCCEEEEECEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
TSKNLYEVLIMDNISWDKLAAIEENSLNLPGIYIDCMEYRTYTYPLSTSHILGYTAWPND
CCCCEEEEEEECCCCHHHEEEECCCCCCCCEEEEEEEECEEEEECCCCCCEEEEECCCCC
AERVNLNIEHTRIQIGKRGLEKHFEEDLRGTCGFEKIEVDARGLKVRELDTIPSIPGKDI
CCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCCCCCE
ALSIDVNLQNKLYEMFDDRVGAAILTDVNTGKIKALVSVPGYNINAFPRRISTKDWKVLL
EEEEEECHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEE
DNPNLPLVSRASQSLYPPGSIFQLVTLLAALEAGVNPKLTVYCNGKSFMNDTNFSCWNHR
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEECCC
GHGNVDMLSAIKYSCNNYIYHLASLIGYNAIFAMARKLGFWRRAVVNLPNELVGNLPTPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCH
WKKRKTKQRWMLGDTLNVAIGQGYITTTPLQLVRMITSIANGKQLLQLSLTLDEDGYHKN
HHHHHHHHHEECCCEEEEEECCCEEEECHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCE
LNINAGHIDFIRQALKAVINNESSGTAYSLDSDKTKLMISGKTGSAQVGSSLQSQAAHGL
ECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCHHHCCHHHHHCCCEE
FVGFSTTNKPEYAVAVILEHGGQIGIKSASAIAKSILIEASTL
EEEECCCCCCCEEEEEEEECCCCEEECHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]