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The map label for this gene is acnA [H]

Identifier: 189183635

GI number: 189183635

Start: 752752

End: 753150

Strand: Direct

Name: acnA [H]

Synonym: OTT_0728

Alternate gene names: 189183635

Gene position: 752752-753150 (Clockwise)

Preceding gene: 189183634

Following gene: 189183638

Centisome position: 37.47

GC content: 32.33

Gene sequence:

>399_bases
ATGACACCACTTGTCCTACCTGAGATCATAGGAGTAAAGCTAACCAACTCCCTAAGAGCAGGAGTTAATGCTACAGATTT
GGTACTTACAGTTACTAAAATACTGCGTGAAAAAGGCGTAGTAGGTAAATTTGTTGAGTTCTTTGGAACAAGAGTAGATA
ATTTATCTTTGCCTAATAGAGCTATTATTAGTAATATGTGTCCTGAATTTGGAGCAACTTGTGCTTATTTTCCAATCGAT
CAAGAAATAATAAAACACCTTACTTTAACTGGTAGAAAGAGCGAGGATATTGAATTAGTTGAAAAATATGCAAAAAAGCA
ATTATTGTGGCGTAACACTAACGATGAAATTATTATAATAGTTGTAATGTTCAAATTAAGTCATTACCATATTTTGTAA

Upstream 100 bases:

>100_bases
CTTAGTTGGGCTAGATAGTCATACAACTATGGCTAATGGATTAGGTGCGCTTTGGTTGGGGATAGGTAGCATTGAAGCAG
AAGCTGCTATGCTAGGATGA

Downstream 100 bases:

>100_bases
CAACAAGAATAGAATAAGCAAAAAGAAGAGTAGATAAAATTTGAATTAAGTTTGGAGATAAGAATAACTTGCAAAAGAAG
CAATTACATGAACAAAGAAA

Product: aconitate hydratase

Products: NA

Alternate protein names: Aconitase 1; Citrate hydro-lyase 1 [H]

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MTPLVLPEIIGVKLTNSLRAGVNATDLVLTVTKILREKGVVGKFVEFFGTRVDNLSLPNRAIISNMCPEFGATCAYFPID
QEIIKHLTLTGRKSEDIELVEKYAKKQLLWRNTNDEIIIIVVMFKLSHYHIL

Sequences:

>Translated_132_residues
MTPLVLPEIIGVKLTNSLRAGVNATDLVLTVTKILREKGVVGKFVEFFGTRVDNLSLPNRAIISNMCPEFGATCAYFPID
QEIIKHLTLTGRKSEDIELVEKYAKKQLLWRNTNDEIIIIVVMFKLSHYHIL
>Mature_131_residues
TPLVLPEIIGVKLTNSLRAGVNATDLVLTVTKILREKGVVGKFVEFFGTRVDNLSLPNRAIISNMCPEFGATCAYFPIDQ
EIIKHLTLTGRKSEDIELVEKYAKKQLLWRNTNDEIIIIVVMFKLSHYHIL

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. May have an iron-responsive regulatory function [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Homo sapiens, GI8659555, Length=112, Percent_Identity=50, Blast_Score=119, Evalue=5e-28,
Organism=Homo sapiens, GI41352693, Length=113, Percent_Identity=46.9026548672566, Blast_Score=109, Evalue=7e-25,
Organism=Homo sapiens, GI4501867, Length=94, Percent_Identity=44.6808510638298, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787531, Length=120, Percent_Identity=54.1666666666667, Blast_Score=150, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17568399, Length=113, Percent_Identity=52.212389380531, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25149337, Length=120, Percent_Identity=41.6666666666667, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI32564738, Length=120, Percent_Identity=41.6666666666667, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25149342, Length=100, Percent_Identity=43, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323335, Length=89, Percent_Identity=40.4494382022472, Blast_Score=72, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24645686, Length=112, Percent_Identity=50.8928571428571, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI17137564, Length=109, Percent_Identity=49.5412844036697, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281365315, Length=120, Percent_Identity=37.5, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17864292, Length=120, Percent_Identity=37.5, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI161076999, Length=105, Percent_Identity=38.0952380952381, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28571643, Length=91, Percent_Identity=36.2637362637363, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR006249
- InterPro:   IPR015934
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00330 Aconitase; PF00694 Aconitase_C [H]

EC number: =4.2.1.3 [H]

Molecular weight: Translated: 14942; Mature: 14811

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLVLPEIIGVKLTNSLRAGVNATDLVLTVTKILREKGVVGKFVEFFGTRVDNLSLPNR
CCCCCCHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCH
AIISNMCPEFGATCAYFPIDQEIIKHLTLTGRKSEDIELVEKYAKKQLLWRNTNDEIIII
HHHHHHCHHHCCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VVMFKLSHYHIL
EEEEHHHHHCCC
>Mature Secondary Structure 
TPLVLPEIIGVKLTNSLRAGVNATDLVLTVTKILREKGVVGKFVEFFGTRVDNLSLPNR
CCCCCHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCH
AIISNMCPEFGATCAYFPIDQEIIKHLTLTGRKSEDIELVEKYAKKQLLWRNTNDEIIII
HHHHHHCHHHCCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VVMFKLSHYHIL
EEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1541275; 9097039; 9278503; 1838390; 10585860 [H]