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The map label for this gene is murD [H]

Identifier: 189183580

GI number: 189183580

Start: 701262

End: 702680

Strand: Direct

Name: murD [H]

Synonym: OTT_0673

Alternate gene names: 189183580

Gene position: 701262-702680 (Clockwise)

Preceding gene: 189183578

Following gene: 189183581

Centisome position: 34.91

GC content: 29.88

Gene sequence:

>1419_bases
GTGATTAGACTATATTCACAAAGGAATAAAAAAATAGGTGTGTTTGGATTATCTCGTACCGGTTTAGCAGTTTGTAAATC
TCTAGCTGGACTGGCTGATTTAATTGTTTACGATGATTCACCTGACAGTAGGAATTCTTTCTACAAACATAATATTAGTT
CAAAAGATTACAAGATGATCAACATTGATCAACCAGAATGGCAAGTATGTGACTTTATTATACTAAGCCCTGGTATACCA
TATTATACCACACCTCATCCTATTATAAAGCATGCGCAAAAGTATAACATACCAATTAAATCAGATATAGATCTTCTATA
TAATGAATATCCTAATCGTAAATACATTGGTATTACCGGAACCAATGGAAAAAGTACAACCTCTACTCTTATTCATCATA
TTTTAACGCTAACAAACGGTAAATTTGATCTTGGAGGTAATATAGGTAAAGCTGTTTTATCTTTAAGAAATAATGTAGAA
GGATATGTCTTAGAGCTTTCATCATTTCAACTAGATTTAATAACTGATCTGAAGCTAGATATTGCCGTATTATTAAACAT
CACTGCTGATCATATAGATCGTTATTATTCATTTGACCAATATATACTATCTAAGCAAAAGATACTTTCTCTTGCTACAA
AAAATGGTTATGCTTTAATTAGTAAAGAGTGTTCTATTATTTCTAACGCCAATTACAATACATTATATGCAAGCAAGTTT
AGAAATACTACGTGTAGCTTAGAATTAATAAAGAAAGCTATAAACACTGATGAATTAAATAAATCTATTGCAGATGAAAG
TATAAATTGTCAATCTCAGCCTACATTAGTGAAATTTTCATCTCACCTACCTCAACATATAAATTTTACTTCTTTACTAT
TAGGTAAGCATAATAATCAAAATATTGCTGCTGCTTTTTATGCATGTAAAATTTTAGGTAGAAATGAATATGATATTACT
AGCGCTATAAATCAATTTCAACCTTTACCTCATCGTATGCAATATATAGGAAAAAAAAACAACATACATATTTATAATGA
TAGTAAGGCAACCAATGCAGATGCTGCTAGTAAAGCATTATTAAGCTTATCAAATATCTATTGGATAGCTGGAGGAGTTG
CTAAAGAAGGAGGAGTTAATTCGATAGTAAATACGCTATATGAAGTTAAAAAAGCATATTTATATGGGCAATCTAGATAT
GCTCTTGCAGAAACATTAAAAGGAGTAGTTGCTTTTTCAATTAATAACACCATGGAGGAAGCATTTAATCAAGCGATAAT
AGATGCTGAATCTGATCAAAGTGGGCTAGATAAAAATATATTATTTGCTCCTGCTTGCGCTTCATTTGATCAGTTTAAGA
ACTTTGAAGATAGGGGTAATCAATTTATAAAAATATCTCAAAAATTTATAACAGAATAA

Upstream 100 bases:

>100_bases
TTCTATCCACTCTACCATAAGAAAAACTTTGTTAAGAAAAAATAATAATATAAAATATGCATAACTATTATCAAAAACAA
TTAAATTTATTTAAAAAAAC

Downstream 100 bases:

>100_bases
AAATATATGAACTATAATTCGACTGAAAAGTTTAGAATATTATGGCGCTGGTGGAAAAGTATAGATCAATACACAGTATT
TTTATTATGTATTTTATCTG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MIRLYSQRNKKIGVFGLSRTGLAVCKSLAGLADLIVYDDSPDSRNSFYKHNISSKDYKMINIDQPEWQVCDFIILSPGIP
YYTTPHPIIKHAQKYNIPIKSDIDLLYNEYPNRKYIGITGTNGKSTTSTLIHHILTLTNGKFDLGGNIGKAVLSLRNNVE
GYVLELSSFQLDLITDLKLDIAVLLNITADHIDRYYSFDQYILSKQKILSLATKNGYALISKECSIISNANYNTLYASKF
RNTTCSLELIKKAINTDELNKSIADESINCQSQPTLVKFSSHLPQHINFTSLLLGKHNNQNIAAAFYACKILGRNEYDIT
SAINQFQPLPHRMQYIGKKNNIHIYNDSKATNADAASKALLSLSNIYWIAGGVAKEGGVNSIVNTLYEVKKAYLYGQSRY
ALAETLKGVVAFSINNTMEEAFNQAIIDAESDQSGLDKNILFAPACASFDQFKNFEDRGNQFIKISQKFITE

Sequences:

>Translated_472_residues
MIRLYSQRNKKIGVFGLSRTGLAVCKSLAGLADLIVYDDSPDSRNSFYKHNISSKDYKMINIDQPEWQVCDFIILSPGIP
YYTTPHPIIKHAQKYNIPIKSDIDLLYNEYPNRKYIGITGTNGKSTTSTLIHHILTLTNGKFDLGGNIGKAVLSLRNNVE
GYVLELSSFQLDLITDLKLDIAVLLNITADHIDRYYSFDQYILSKQKILSLATKNGYALISKECSIISNANYNTLYASKF
RNTTCSLELIKKAINTDELNKSIADESINCQSQPTLVKFSSHLPQHINFTSLLLGKHNNQNIAAAFYACKILGRNEYDIT
SAINQFQPLPHRMQYIGKKNNIHIYNDSKATNADAASKALLSLSNIYWIAGGVAKEGGVNSIVNTLYEVKKAYLYGQSRY
ALAETLKGVVAFSINNTMEEAFNQAIIDAESDQSGLDKNILFAPACASFDQFKNFEDRGNQFIKISQKFITE
>Mature_472_residues
MIRLYSQRNKKIGVFGLSRTGLAVCKSLAGLADLIVYDDSPDSRNSFYKHNISSKDYKMINIDQPEWQVCDFIILSPGIP
YYTTPHPIIKHAQKYNIPIKSDIDLLYNEYPNRKYIGITGTNGKSTTSTLIHHILTLTNGKFDLGGNIGKAVLSLRNNVE
GYVLELSSFQLDLITDLKLDIAVLLNITADHIDRYYSFDQYILSKQKILSLATKNGYALISKECSIISNANYNTLYASKF
RNTTCSLELIKKAINTDELNKSIADESINCQSQPTLVKFSSHLPQHINFTSLLLGKHNNQNIAAAFYACKILGRNEYDIT
SAINQFQPLPHRMQYIGKKNNIHIYNDSKATNADAASKALLSLSNIYWIAGGVAKEGGVNSIVNTLYEVKKAYLYGQSRY
ALAETLKGVVAFSINNTMEEAFNQAIIDAESDQSGLDKNILFAPACASFDQFKNFEDRGNQFIKISQKFITE

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=412, Percent_Identity=31.7961165048544, Blast_Score=155, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 52854; Mature: 52854

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLYSQRNKKIGVFGLSRTGLAVCKSLAGLADLIVYDDSPDSRNSFYKHNISSKDYKMI
CEEEECCCCCEEEEEEECCHHHHHHHHHHCCEEEEEECCCCCCCCCCEECCCCCCCEEEE
NIDQPEWQVCDFIILSPGIPYYTTPHPIIKHAQKYNIPIKSDIDLLYNEYPNRKYIGITG
ECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEEC
TNGKSTTSTLIHHILTLTNGKFDLGGNIGKAVLSLRNNVEGYVLELSSFQLDLITDLKLD
CCCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCEEEEEECCCEEEEEECCCEE
IAVLLNITADHIDRYYSFDQYILSKQKILSLATKNGYALISKECSIISNANYNTLYASKF
EEHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCEEEHHHH
RNTTCSLELIKKAINTDELNKSIADESINCQSQPTLVKFSSHLPQHINFTSLLLGKHNNQ
CCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCEEEEHHCCCCCCCHHHHEEECCCCC
NIAAAFYACKILGRNEYDITSAINQFQPLPHRMQYIGKKNNIHIYNDSKATNADAASKAL
CHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
LSLSNIYWIAGGVAKEGGVNSIVNTLYEVKKAYLYGQSRYALAETLKGVVAFSINNTMEE
HHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEECHHHHH
AFNQAIIDAESDQSGLDKNILFAPACASFDQFKNFEDRGNQFIKISQKFITE
HHHHHEECCCCCCCCCCCCEEEECCCCCHHHHCCHHHCCCEEEEEEHHHHCC
>Mature Secondary Structure
MIRLYSQRNKKIGVFGLSRTGLAVCKSLAGLADLIVYDDSPDSRNSFYKHNISSKDYKMI
CEEEECCCCCEEEEEEECCHHHHHHHHHHCCEEEEEECCCCCCCCCCEECCCCCCCEEEE
NIDQPEWQVCDFIILSPGIPYYTTPHPIIKHAQKYNIPIKSDIDLLYNEYPNRKYIGITG
ECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEEC
TNGKSTTSTLIHHILTLTNGKFDLGGNIGKAVLSLRNNVEGYVLELSSFQLDLITDLKLD
CCCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCEEEEEECCCEEEEEECCCEE
IAVLLNITADHIDRYYSFDQYILSKQKILSLATKNGYALISKECSIISNANYNTLYASKF
EEHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCEEEHHHH
RNTTCSLELIKKAINTDELNKSIADESINCQSQPTLVKFSSHLPQHINFTSLLLGKHNNQ
CCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCEEEEHHCCCCCCCHHHHEEECCCCC
NIAAAFYACKILGRNEYDITSAINQFQPLPHRMQYIGKKNNIHIYNDSKATNADAASKAL
CHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
LSLSNIYWIAGGVAKEGGVNSIVNTLYEVKKAYLYGQSRYALAETLKGVVAFSINNTMEE
HHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEECHHHHH
AFNQAIIDAESDQSGLDKNILFAPACASFDQFKNFEDRGNQFIKISQKFITE
HHHHHEECCCCCCCCCCCCEEEECCCCCHHHHCCHHHCCCEEEEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA