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The map label for this gene is dnaB [H]

Identifier: 189183375

GI number: 189183375

Start: 492154

End: 492633

Strand: Direct

Name: dnaB [H]

Synonym: OTT_0468

Alternate gene names: 189183375

Gene position: 492154-492633 (Clockwise)

Preceding gene: 189183374

Following gene: 189183377

Centisome position: 24.5

GC content: 31.87

Gene sequence:

>480_bases
ATGGAAAAAGATCTTGCAAAGATTGCTCCAAATAATATTCAAGCAGAGCAAATGATACTTGGAGCAATTCTGATTAACAA
TCGTGCGCTATATAACATTAACGAATTTTTACTGCCAGAACATTTTTATGAACCATTACATGGTAAAATATACAAGTCAA
TTAATCTCATTATTAGTAAAGGAATTAGTGCTACTGTAATTTCGCTCAAAAATATGCTAGGCAATGAACCCTCATTTGAT
GAAATAGGTGGAGTAGATTATCTAGCTAAACTTACAACTTTAGCATTAAGTATAGTTAATGTTAATGAGTACGGCAAAAT
AGTATATGACCTTGCGCTGCGGCGTTATTTAATTGAAATTGGAGAAAAAATAGTAACAAATGCGTATTCTTCTACTTTAG
CAGATTTAGCTATAAGTCAGATCGAAACTGCTGAATCTCAATTATATGATCTAGGTGCAAGAGGAACTTTAAGTAAATGA

Upstream 100 bases:

>100_bases
CAAGCAACCATATGGAAAATCTTGCGCACGCTTTTAAAGCTCAAGGGTTCTCTTTTGAGTTAGTTAAGTTTAGTAATTTT
AATAAAATTTAAAGGGTGAT

Downstream 100 bases:

>100_bases
TTTACAAAATTACAAACTTCAATTGAAGAATCATGGACATCAATTTCATCTGCTATTAAAAATAAAAACTCTATTAACGG
TATTAGTAGTGGACTACTTG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MEKDLAKIAPNNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNEPSFD
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLGARGTLSK

Sequences:

>Translated_159_residues
MEKDLAKIAPNNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNEPSFD
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLGARGTLSK
>Mature_159_residues
MEKDLAKIAPNNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNEPSFD
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLGARGTLSK

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=148, Percent_Identity=29.0540540540541, Blast_Score=72, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 17592; Mature: 17592

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLAKIAPNNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHHCCCEEECHHHHHCCHHHHCHHHHHHHHHHHHHHHC
GISATVISLKNMLGNEPSFDEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAISQIETAESQLYDLGARGTLSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEKDLAKIAPNNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHHCCCEEECHHHHHCCHHHHCHHHHHHHHHHHHHHHC
GISATVISLKNMLGNEPSFDEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAISQIETAESQLYDLGARGTLSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA