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The map label for this gene is alaS

Identifier: 189026240

GI number: 189026240

Start: 1109382

End: 1111199

Strand: Reverse

Name: alaS

Synonym: TPASS_1017

Alternate gene names: 189026240

Gene position: 1111199-1109382 (Counterclockwise)

Preceding gene: 189026245

Following gene: 189026239

Centisome position: 97.52

GC content: 54.51

Gene sequence:

>1818_bases
ATGAGCATTCCTATTCGCGCCGATCAGCTACGCTCGCGTTATCTCGCCTTCTTTAGCCAGAAGGCTCATGTGGTTATCTC
GGGTAAGTCCCTTGTGCCCGAGCACGATCCTACCGTGCTTTTTACTACTGCTGGCATGCATCCGTTGGTTCCCTATCTTA
TGGGTGAGCCACATCCGGCTGGTACCCGCTTGGTCAATGCGCAAAAGTGTCTGCGCACCGGTGACATCGATGCGGTGGGC
GATAACTCGCATCTGACCTTTTTCGAGATGTTGGGTAACTGGTCGTTGGGCGATTACTTCAAGGAGGAGGCAATCGCGTT
CAGCTTTGAATTTCTTACTGGTGCTCCATGGCTCGGCATATCCCCTGACCGGCTTTCGGTGACTGTTTTTGCAGGGGATG
AGGCTGTTGCGCGTGATGAGGAATCTGCCGCTATCTGGGAACGACTTGGTATCGCGCGCACCCATATTCATTTTTTACCA
CGTGCGGATAACTGGTGGGGACCTACGGGGGAAACAGGGCCATGTGGTCCGGATACCGAGATATTCTTTGATACTGGAGT
TCCTCCTTGCAGTGTGAGCTGTCGTCCGGGATGTTCTTGCGGCAAGTACGTAGAGATTTGGAATGACGTGTTCATGCAGT
ATCGTAAGGACGCAGACGGCAGGTACCGGCCGCTTGAACGTTATTGTGTGGACACCGGTATGGGTATTGAACGCACCGTC
GCGGTTTTGCAGGGCAAGCGGTCAGTGTATGATACGGAGATCTTTACCCCGCTCCTGGAGCGGATAGGTCAGTTATGTGG
GAAGAGGTATGGGTGTCAGGGAGCGCACGACGTATCCATGCGGATTGTATGCGATCATATCCGTGCGGCCACTTTTATCC
TCGGCGATCCTGTCCCTGTACGCCCATCTAACGTGGGTGCAGGCTACGTACTGCGCAGAATCATTCGCCGTTCAGTCCGC
CACGGAAGGAAGCTCGGTATCGATGGGGAGTTCCTGTCCTCCCTCGCGCGCGTGGTGATTGGGCAATACGCTGCGGTATA
CCCGGAACTGGAAGAGAAAGCTACGTGTATTGCGCAGGAGCTTGCAAACGAGGAACGTAAGTTCCTCGACGCCCTCAGGA
AGGGTGAGGCAGAGTACGAGAGGATGATACCCAAGTTTTTACAGGGGACAGAGCGAGAAATACCTGGGTCAGTTGCCTTT
CGGCTATACGATACGTATGGATTCCCCCTTGAGCTGACCGAGGAACTTGCGCGTGAATCGGGTTTACGGGTGGACCGTGC
AGGGTTTGACACCGCGTTTCAGGCGCACCAGGCGTGCTCTCGGATCGGTGCACAGCGAGTATTTAAGGGTGGGTTGGCTG
ATCATTCAGCGGAAACGACAGCGTACCACACGGCGACGCATCTGCTGCACCAGGCATTGCGAGTGGTTCTTGGTACGCAC
GTGCAGCAAAAAGGCTCAAATATTACGGCAGAACGCCTGCGTTTCGACTTTTCTCACCCAAGACCGATGAGTGCGCAGGA
GAAGGTGCAGGTGGAACAGTTGGTGAATGAGCAGATCCGGGCAGACTTGCCGGTGTGCTGTGAAGTGATGTCGCTCGAAG
ATGCGATGAATTCTGGTGCCGTGGCGTTGTTTGGTGAGAAATACGAATCAACTGTAAAGGTGTACTCCATAGGAACTTTT
TCTCGGGAAGTGTGTGGCGGTCCACATGTAGCACGTACCGGGCAGCTCGGGCGGTTTTCTATTCAAAAGGAACAGTCGAG
TGCGGCGGGGGTGCGGCGTATTCGCGCGATTCTTGAGAAAAGCGGGGAAAAGTCGTAA

Upstream 100 bases:

>100_bases
TCAAGGATCTTGTCGCAAGGTTGCTCAGAGAGTCCCCGTTTCTTTTTCCCGTTTAGGGTCAGATGTTGACCGCAGCAGGG
AAAAGGCGATAATACCCTCC

Downstream 100 bases:

>100_bases
CTTGCCCTGCTGCGTATAGTTTCTCCGGCGTGTGTGCTGGTGACTGAGTCGCGGTTGTTACGGGGAGTTCTCATGGGCAG
ATACATAGTTCCCGCTCTGT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MSIPIRADQLRSRYLAFFSQKAHVVISGKSLVPEHDPTVLFTTAGMHPLVPYLMGEPHPAGTRLVNAQKCLRTGDIDAVG
DNSHLTFFEMLGNWSLGDYFKEEAIAFSFEFLTGAPWLGISPDRLSVTVFAGDEAVARDEESAAIWERLGIARTHIHFLP
RADNWWGPTGETGPCGPDTEIFFDTGVPPCSVSCRPGCSCGKYVEIWNDVFMQYRKDADGRYRPLERYCVDTGMGIERTV
AVLQGKRSVYDTEIFTPLLERIGQLCGKRYGCQGAHDVSMRIVCDHIRAATFILGDPVPVRPSNVGAGYVLRRIIRRSVR
HGRKLGIDGEFLSSLARVVIGQYAAVYPELEEKATCIAQELANEERKFLDALRKGEAEYERMIPKFLQGTEREIPGSVAF
RLYDTYGFPLELTEELARESGLRVDRAGFDTAFQAHQACSRIGAQRVFKGGLADHSAETTAYHTATHLLHQALRVVLGTH
VQQKGSNITAERLRFDFSHPRPMSAQEKVQVEQLVNEQIRADLPVCCEVMSLEDAMNSGAVALFGEKYESTVKVYSIGTF
SREVCGGPHVARTGQLGRFSIQKEQSSAAGVRRIRAILEKSGEKS

Sequences:

>Translated_605_residues
MSIPIRADQLRSRYLAFFSQKAHVVISGKSLVPEHDPTVLFTTAGMHPLVPYLMGEPHPAGTRLVNAQKCLRTGDIDAVG
DNSHLTFFEMLGNWSLGDYFKEEAIAFSFEFLTGAPWLGISPDRLSVTVFAGDEAVARDEESAAIWERLGIARTHIHFLP
RADNWWGPTGETGPCGPDTEIFFDTGVPPCSVSCRPGCSCGKYVEIWNDVFMQYRKDADGRYRPLERYCVDTGMGIERTV
AVLQGKRSVYDTEIFTPLLERIGQLCGKRYGCQGAHDVSMRIVCDHIRAATFILGDPVPVRPSNVGAGYVLRRIIRRSVR
HGRKLGIDGEFLSSLARVVIGQYAAVYPELEEKATCIAQELANEERKFLDALRKGEAEYERMIPKFLQGTEREIPGSVAF
RLYDTYGFPLELTEELARESGLRVDRAGFDTAFQAHQACSRIGAQRVFKGGLADHSAETTAYHTATHLLHQALRVVLGTH
VQQKGSNITAERLRFDFSHPRPMSAQEKVQVEQLVNEQIRADLPVCCEVMSLEDAMNSGAVALFGEKYESTVKVYSIGTF
SREVCGGPHVARTGQLGRFSIQKEQSSAAGVRRIRAILEKSGEKS
>Mature_604_residues
SIPIRADQLRSRYLAFFSQKAHVVISGKSLVPEHDPTVLFTTAGMHPLVPYLMGEPHPAGTRLVNAQKCLRTGDIDAVGD
NSHLTFFEMLGNWSLGDYFKEEAIAFSFEFLTGAPWLGISPDRLSVTVFAGDEAVARDEESAAIWERLGIARTHIHFLPR
ADNWWGPTGETGPCGPDTEIFFDTGVPPCSVSCRPGCSCGKYVEIWNDVFMQYRKDADGRYRPLERYCVDTGMGIERTVA
VLQGKRSVYDTEIFTPLLERIGQLCGKRYGCQGAHDVSMRIVCDHIRAATFILGDPVPVRPSNVGAGYVLRRIIRRSVRH
GRKLGIDGEFLSSLARVVIGQYAAVYPELEEKATCIAQELANEERKFLDALRKGEAEYERMIPKFLQGTEREIPGSVAFR
LYDTYGFPLELTEELARESGLRVDRAGFDTAFQAHQACSRIGAQRVFKGGLADHSAETTAYHTATHLLHQALRVVLGTHV
QQKGSNITAERLRFDFSHPRPMSAQEKVQVEQLVNEQIRADLPVCCEVMSLEDAMNSGAVALFGEKYESTVKVYSIGTFS
REVCGGPHVARTGQLGRFSIQKEQSSAAGVRRIRAILEKSGEKS

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI109148542, Length=450, Percent_Identity=41.1111111111111, Blast_Score=310, Evalue=3e-84,
Organism=Homo sapiens, GI38569417, Length=463, Percent_Identity=38.4449244060475, Blast_Score=266, Evalue=4e-71,
Organism=Escherichia coli, GI1789048, Length=451, Percent_Identity=40.7982261640798, Blast_Score=322, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17506981, Length=449, Percent_Identity=40.7572383073497, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17536681, Length=451, Percent_Identity=38.3592017738359, Blast_Score=275, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6324911, Length=461, Percent_Identity=39.9132321041215, Blast_Score=322, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24582809, Length=477, Percent_Identity=39.622641509434, Blast_Score=315, Evalue=6e-86,
Organism=Drosophila melanogaster, GI45552267, Length=477, Percent_Identity=39.622641509434, Blast_Score=315, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24658214, Length=499, Percent_Identity=33.0661322645291, Blast_Score=229, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYA_TREPA (O83980)

Other databases:

- EMBL:   AE000520
- PIR:   E71253
- RefSeq:   NP_219454.1
- ProteinModelPortal:   O83980
- IntAct:   O83980
- GeneID:   2610864
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP1017
- NMPDR:   fig|243276.1.peg.1012
- TIGR:   TP_1017
- HOGENOM:   HBG354397
- OMA:   RKCVDTG
- ProtClustDB:   PRK01584
- BioCyc:   TPAL243276:TP_1017-MONOMER
- BRENDA:   6.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00036_B
- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947
- PRINTS:   PR00980
- SMART:   SM00863
- TIGRFAMs:   TIGR00344

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD; SSF101353 Ala-tRNA-synth_IIc_anticod-bd; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.7

Molecular weight: Translated: 67188; Mature: 67057

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPIRADQLRSRYLAFFSQKAHVVISGKSLVPEHDPTVLFTTAGMHPLVPYLMGEPHPA
CCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCC
GTRLVNAQKCLRTGDIDAVGDNSHLTFFEMLGNWSLGDYFKEEAIAFSFEFLTGAPWLGI
CCCEECHHHHHHCCCCCCCCCCCCEEHHHHHCCCCHHHHHHHHHHEEEEHHHCCCCCCCC
SPDRLSVTVFAGDEAVARDEESAAIWERLGIARTHIHFLPRADNWWGPTGETGPCGPDTE
CCCCEEEEEEECCHHHHCCCHHHHHHHHHCHHHHHEEEECCCCCCCCCCCCCCCCCCCCE
IFFDTGVPPCSVSCRPGCSCGKYVEIWNDVFMQYRKDADGRYRPLERYCVDTGMGIERTV
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHH
AVLQGKRSVYDTEIFTPLLERIGQLCGKRYGCQGAHDVSMRIVCDHIRAATFILGDPVPV
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCC
RPSNVGAGYVLRRIIRRSVRHGRKLGIDGEFLSSLARVVIGQYAAVYPELEEKATCIAQE
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LANEERKFLDALRKGEAEYERMIPKFLQGTEREIPGSVAFRLYDTYGFPLELTEELARES
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHC
GLRVDRAGFDTAFQAHQACSRIGAQRVFKGGLADHSAETTAYHTATHLLHQALRVVLGTH
CCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VQQKGSNITAERLRFDFSHPRPMSAQEKVQVEQLVNEQIRADLPVCCEVMSLEDAMNSGA
HHHCCCCCCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCE
VALFGEKYESTVKVYSIGTFSREVCGGPHVARTGQLGRFSIQKEQSSAAGVRRIRAILEK
EEEECCCCCCEEEEEEECCCHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHH
SGEKS
CCCCC
>Mature Secondary Structure 
SIPIRADQLRSRYLAFFSQKAHVVISGKSLVPEHDPTVLFTTAGMHPLVPYLMGEPHPA
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCC
GTRLVNAQKCLRTGDIDAVGDNSHLTFFEMLGNWSLGDYFKEEAIAFSFEFLTGAPWLGI
CCCEECHHHHHHCCCCCCCCCCCCEEHHHHHCCCCHHHHHHHHHHEEEEHHHCCCCCCCC
SPDRLSVTVFAGDEAVARDEESAAIWERLGIARTHIHFLPRADNWWGPTGETGPCGPDTE
CCCCEEEEEEECCHHHHCCCHHHHHHHHHCHHHHHEEEECCCCCCCCCCCCCCCCCCCCE
IFFDTGVPPCSVSCRPGCSCGKYVEIWNDVFMQYRKDADGRYRPLERYCVDTGMGIERTV
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHH
AVLQGKRSVYDTEIFTPLLERIGQLCGKRYGCQGAHDVSMRIVCDHIRAATFILGDPVPV
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCC
RPSNVGAGYVLRRIIRRSVRHGRKLGIDGEFLSSLARVVIGQYAAVYPELEEKATCIAQE
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LANEERKFLDALRKGEAEYERMIPKFLQGTEREIPGSVAFRLYDTYGFPLELTEELARES
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHC
GLRVDRAGFDTAFQAHQACSRIGAQRVFKGGLADHSAETTAYHTATHLLHQALRVVLGTH
CCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VQQKGSNITAERLRFDFSHPRPMSAQEKVQVEQLVNEQIRADLPVCCEVMSLEDAMNSGA
HHHCCCCCCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCE
VALFGEKYESTVKVYSIGTFSREVCGGPHVARTGQLGRFSIQKEQSSAAGVRRIRAILEK
EEEECCCCCCEEEEEEECCCHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHH
SGEKS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876