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The map label for this gene is est [H]

Identifier: 189026158

GI number: 189026158

Start: 1017917

End: 1018855

Strand: Reverse

Name: est [H]

Synonym: TPASS_0935

Alternate gene names: 189026158

Gene position: 1018855-1017917 (Counterclockwise)

Preceding gene: 189026159

Following gene: 189026156

Centisome position: 89.42

GC content: 50.37

Gene sequence:

>939_bases
ATGGGACCTTCGCGCGCTCACGTGCACCGAATGGCGAAAAAACTGTTTAAGAAGATGCGCTTTACCCCGCGTACATCGGT
TTCTGCCTTGCGTGCGGGGTATGATGACTTTTTTTATATGCCGTATGTGCCCAATGGGGTGGATGTTTCCTGTGAGGTAT
TGGCTAATATCGAAACAGATGTGTTGAGCCCGCGGTTCCTAATGGTTGGCCATGCAGTGTTGTACGCACATGGTGGATTT
TTTATAAGTGGTTCTCCTATGGCTTCTCGTGCGCTCTGTGCATCTTTAGCGGATGAAGTCGGCGCGAAGTTTTATATTCC
TGCATATCAGCTTGCGCCCGAGCATCCTTTTCCTGCTGCACTAGAAAATATGTATGATGCGTATGTAGCACTTATTGATC
AGAAGAAATTTCGTCCTGAAGATATTATCTTCAGTGGGGATGGAGCAGGTGCAGGGTTGATGCTTGCACTGACGCACTAT
TTAAAACAGAAGGGGCTGGTGTGTCCGCGCGCTTTGGCGCTTATTTCTCCTTGGGTTGATTTAACTGAGGATCGGTTTGC
AGGGGAGGGAGTTAAGAAGAAAGAACTCCTGTTGACGCGCGATATGTTCCGCTACTGTGCGTTGCAGTACACGTACGCGG
ATAATTTTCGCAATTCACTGGTTTCCCCCCTTTTTGGGAATTTTGGAGATTTCCCTCCGGTGTTTGTGCAATGTGGGGAG
CAAGAGTTGTTCCACACAGACGCATGTGCGCTTGCGCAGATACTTGAAGGGGCGGGGGTACAAGTGACCCTAGACGTGTG
GCCGGGGATGTGGCACCTGTTTCAGTCGATGGAAAGTGTGTCGCCGTGTGCGCACATAGCGGTTGCGCGTATGGGGGCGT
GGGCGCATCGAATGCTGGAGTCTCCTGCTATTGCAGTGCAATCACTAGAGCTCAGATAA

Upstream 100 bases:

>100_bases
GGGGCCGAGCGTAACGGGAATGCGCCGGACGTGTCAAGCGCTCTTATGGGCGTGAAGCGGTTGCCTGACAGAACCAGTTT
CTTTGTGTTACGATAAACGC

Downstream 100 bases:

>100_bases
AGAAACGCACCGCGCGGTGGCGCACAGTTCCAATTCGTTCTGCGCTTATTGCGGTGCAGATGAGGGCGTTTAGGGTATCC
AGCGTTGTGGCGATGCTTCC

Product: N-acetylphosphinothricin-tripetide-deacetylase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MGPSRAHVHRMAKKLFKKMRFTPRTSVSALRAGYDDFFYMPYVPNGVDVSCEVLANIETDVLSPRFLMVGHAVLYAHGGF
FISGSPMASRALCASLADEVGAKFYIPAYQLAPEHPFPAALENMYDAYVALIDQKKFRPEDIIFSGDGAGAGLMLALTHY
LKQKGLVCPRALALISPWVDLTEDRFAGEGVKKKELLLTRDMFRYCALQYTYADNFRNSLVSPLFGNFGDFPPVFVQCGE
QELFHTDACALAQILEGAGVQVTLDVWPGMWHLFQSMESVSPCAHIAVARMGAWAHRMLESPAIAVQSLELR

Sequences:

>Translated_312_residues
MGPSRAHVHRMAKKLFKKMRFTPRTSVSALRAGYDDFFYMPYVPNGVDVSCEVLANIETDVLSPRFLMVGHAVLYAHGGF
FISGSPMASRALCASLADEVGAKFYIPAYQLAPEHPFPAALENMYDAYVALIDQKKFRPEDIIFSGDGAGAGLMLALTHY
LKQKGLVCPRALALISPWVDLTEDRFAGEGVKKKELLLTRDMFRYCALQYTYADNFRNSLVSPLFGNFGDFPPVFVQCGE
QELFHTDACALAQILEGAGVQVTLDVWPGMWHLFQSMESVSPCAHIAVARMGAWAHRMLESPAIAVQSLELR
>Mature_311_residues
GPSRAHVHRMAKKLFKKMRFTPRTSVSALRAGYDDFFYMPYVPNGVDVSCEVLANIETDVLSPRFLMVGHAVLYAHGGFF
ISGSPMASRALCASLADEVGAKFYIPAYQLAPEHPFPAALENMYDAYVALIDQKKFRPEDIIFSGDGAGAGLMLALTHYL
KQKGLVCPRALALISPWVDLTEDRFAGEGVKKKELLLTRDMFRYCALQYTYADNFRNSLVSPLFGNFGDFPPVFVQCGEQ
ELFHTDACALAQILEGAGVQVTLDVWPGMWHLFQSMESVSPCAHIAVARMGAWAHRMLESPAIAVQSLELR

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34598; Mature: 34467

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPSRAHVHRMAKKLFKKMRFTPRTSVSALRAGYDDFFYMPYVPNGVDVSCEVLANIETD
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCHHHEECCHHH
VLSPRFLMVGHAVLYAHGGFFISGSPMASRALCASLADEVGAKFYIPAYQLAPEHPFPAA
HCCCHHHHHHHHHHEECCCEEEECCCHHHHHHHHHHHHHHCCEEEECHHHCCCCCCCHHH
LENMYDAYVALIDQKKFRPEDIIFSGDGAGAGLMLALTHYLKQKGLVCPRALALISPWVD
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCHH
LTEDRFAGEGVKKKELLLTRDMFRYCALQYTYADNFRNSLVSPLFGNFGDFPPVFVQCGE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCC
QELFHTDACALAQILEGAGVQVTLDVWPGMWHLFQSMESVSPCAHIAVARMGAWAHRMLE
HHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
SPAIAVQSLELR
CCCHHHHCCCCC
>Mature Secondary Structure 
GPSRAHVHRMAKKLFKKMRFTPRTSVSALRAGYDDFFYMPYVPNGVDVSCEVLANIETD
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCHHHEECCHHH
VLSPRFLMVGHAVLYAHGGFFISGSPMASRALCASLADEVGAKFYIPAYQLAPEHPFPAA
HCCCHHHHHHHHHHEECCCEEEECCCHHHHHHHHHHHHHHCCEEEECHHHCCCCCCCHHH
LENMYDAYVALIDQKKFRPEDIIFSGDGAGAGLMLALTHYLKQKGLVCPRALALISPWVD
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCHH
LTEDRFAGEGVKKKELLLTRDMFRYCALQYTYADNFRNSLVSPLFGNFGDFPPVFVQCGE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCC
QELFHTDACALAQILEGAGVQVTLDVWPGMWHLFQSMESVSPCAHIAVARMGAWAHRMLE
HHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
SPAIAVQSLELR
CCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]