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The map label for this gene is murE

Identifier: 189026156

GI number: 189026156

Start: 1014943

End: 1016565

Strand: Reverse

Name: murE

Synonym: TPASS_0933

Alternate gene names: 189026156

Gene position: 1016565-1014943 (Counterclockwise)

Preceding gene: 189026158

Following gene: 189026155

Centisome position: 89.21

GC content: 51.32

Gene sequence:

>1623_bases
ATGCTGTACCGTAAACCCATTACTGTCTGTCTTGCCTCCTGTTCTGTGCAAGAAACGCACGGTAGTGCGTGCGACGTTTC
TTCCATAGCGTATGATTCACGCGCAGTGCGTGAGGGTTCGGTTTTTTTTGCGTTGCGTGGTACGCATGCGCACGGCGCGC
AGTACATCCACGCAGCAATTGATGCCGGCGCCTGCGCAATTGTGCATGATTGTCCATTAGATACGTATGTCGTCGGCGTG
TACTATGCTCGTGTACCAGATGCACGGTGTGCGCTTTCTAGTGCTGCTGCAGCTTTCTACGATTTTCCTACGCGTGCTTT
GACAGTTATCGGGGTCACTGGCACGGAGGGTAAAAGCAGCACGGTTAGTTTCATTGCGCAGCTTTTGCGTTTATGTGGTA
AACGAGTTGGTTTTATCTCTACTGTGGAGTATTCGTTAGGAGATGATATTCTCCCGAACGCAGAGCATCAAACCACACCG
GAGTCGCTAACAGTACAGCGGCTCCTCGCTGAGATGCGGGAGCACAGCTGTGAATTTGCAGTAATTGAAGCGTCGTCCCA
CGGGCTTTCCACACGTACGGCACGTTTGCAGGATGTTGCATTTGATGTGGCAGTATGTATGAACGTACGCCATGAACATT
TAGAATTTCATGGTTCGTTTGAACAATATCGCTTTGATAAAGCAAATGTATTTCGTGCGCTAGATGCGCATGATCACATA
AAAGACGGACGGCGCGTTCCTTCTTTTGGAGTACTCTGGGCGGAAGACGCATCGGCTGTGTATTTTCGCGAAGCGACGCA
CAAACCCTGTTTTTTCTTTAAGAGAGGAACCGGCGCTGAGCAAAGGACAGCAGCCTGCCTGGAAAGGATGCCGTGTACCC
TGCTATGGGTGCAAACACTTCCTCAGATATCTCAGGCCTTGCGATTAAGGTTTGTGTTGTCAACTGTTCAGGAACCTGCA
CAACCAGCACAGGACGGAGCACATGATGTATCGGTGCCGCTTGAAGGAGCTTTCAATGCCTGTAATATAGCAGCGTCTTT
TCTTGTACTGCATGGATTGCTTGGTACGTCGCTTGCGGCTTTTGCACAGCACGTGCAGTACGTCCAGCCAATCCAGGGGA
GAATGCAGCGTGTGGATATGGGACAGGATTTTGAGGTGCTCATTGACTATGCGCATACACCCTCTTCCTTCGAGGAGATT
TTGCCACCGCTTGCAGCGCGTGTGCGCGCGCGTAAGAGGCGCATGTTGGTATTGTTCGGCTCGGCCGGGGAGCGGGACAC
GCAAAAGCGTGCAATGCAAGGCGCAATTGCCTCTCGTTATGCGCACGTAATTGTGCTCACAGATGAGGATCCGAGAGGAG
AGGATCCAATGGGTATTCTGTGCATGATTGCGGCAGGTTGTGAGCACAAGAAATTGGGTAAAACCCTGTTTCTGATTCCG
GATCGTGTCGCTGCTCTGCGGCACATATTCTCTCTTGCGCGTGCGCAGGACCTTGTGCTGCTGCTGGGTAAGGGGCATGA
GCATTCAATTATTTATGCGCACACCGTGCAACCGTATGATGAAGAACGTACGGCGCGTGAATTATTACGTGCCAGCTTAT
CCTCTGACACCTTACTATCCTAG

Upstream 100 bases:

>100_bases
AACGAGATGGCCTTTCCCCACCGTTTGCGATCTTCTTTGAAAAAGCATTGACAATAGATTGTTCCCCGAGGGCGTGCAAT
CAGATACAATCAGACCCGCG

Downstream 100 bases:

>100_bases
TTCGTCTGGATGCCGGTGTGCACATCGCGTAGCTTACAGGGTGGAAAGGTAGATACTCACTTTCCCCTGCATGTGCTACA
CTCCGTCGCCTGTGATGTGT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MLYRKPITVCLASCSVQETHGSACDVSSIAYDSRAVREGSVFFALRGTHAHGAQYIHAAIDAGACAIVHDCPLDTYVVGV
YYARVPDARCALSSAAAAFYDFPTRALTVIGVTGTEGKSSTVSFIAQLLRLCGKRVGFISTVEYSLGDDILPNAEHQTTP
ESLTVQRLLAEMREHSCEFAVIEASSHGLSTRTARLQDVAFDVAVCMNVRHEHLEFHGSFEQYRFDKANVFRALDAHDHI
KDGRRVPSFGVLWAEDASAVYFREATHKPCFFFKRGTGAEQRTAACLERMPCTLLWVQTLPQISQALRLRFVLSTVQEPA
QPAQDGAHDVSVPLEGAFNACNIAASFLVLHGLLGTSLAAFAQHVQYVQPIQGRMQRVDMGQDFEVLIDYAHTPSSFEEI
LPPLAARVRARKRRMLVLFGSAGERDTQKRAMQGAIASRYAHVIVLTDEDPRGEDPMGILCMIAAGCEHKKLGKTLFLIP
DRVAALRHIFSLARAQDLVLLLGKGHEHSIIYAHTVQPYDEERTARELLRASLSSDTLLS

Sequences:

>Translated_540_residues
MLYRKPITVCLASCSVQETHGSACDVSSIAYDSRAVREGSVFFALRGTHAHGAQYIHAAIDAGACAIVHDCPLDTYVVGV
YYARVPDARCALSSAAAAFYDFPTRALTVIGVTGTEGKSSTVSFIAQLLRLCGKRVGFISTVEYSLGDDILPNAEHQTTP
ESLTVQRLLAEMREHSCEFAVIEASSHGLSTRTARLQDVAFDVAVCMNVRHEHLEFHGSFEQYRFDKANVFRALDAHDHI
KDGRRVPSFGVLWAEDASAVYFREATHKPCFFFKRGTGAEQRTAACLERMPCTLLWVQTLPQISQALRLRFVLSTVQEPA
QPAQDGAHDVSVPLEGAFNACNIAASFLVLHGLLGTSLAAFAQHVQYVQPIQGRMQRVDMGQDFEVLIDYAHTPSSFEEI
LPPLAARVRARKRRMLVLFGSAGERDTQKRAMQGAIASRYAHVIVLTDEDPRGEDPMGILCMIAAGCEHKKLGKTLFLIP
DRVAALRHIFSLARAQDLVLLLGKGHEHSIIYAHTVQPYDEERTARELLRASLSSDTLLS
>Mature_540_residues
MLYRKPITVCLASCSVQETHGSACDVSSIAYDSRAVREGSVFFALRGTHAHGAQYIHAAIDAGACAIVHDCPLDTYVVGV
YYARVPDARCALSSAAAAFYDFPTRALTVIGVTGTEGKSSTVSFIAQLLRLCGKRVGFISTVEYSLGDDILPNAEHQTTP
ESLTVQRLLAEMREHSCEFAVIEASSHGLSTRTARLQDVAFDVAVCMNVRHEHLEFHGSFEQYRFDKANVFRALDAHDHI
KDGRRVPSFGVLWAEDASAVYFREATHKPCFFFKRGTGAEQRTAACLERMPCTLLWVQTLPQISQALRLRFVLSTVQEPA
QPAQDGAHDVSVPLEGAFNACNIAASFLVLHGLLGTSLAAFAQHVQYVQPIQGRMQRVDMGQDFEVLIDYAHTPSSFEEI
LPPLAARVRARKRRMLVLFGSAGERDTQKRAMQGAIASRYAHVIVLTDEDPRGEDPMGILCMIAAGCEHKKLGKTLFLIP
DRVAALRHIFSLARAQDLVLLLGKGHEHSIIYAHTVQPYDEERTARELLRASLSSDTLLS

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=520, Percent_Identity=29.0384615384615, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_TREPA (O83903)

Other databases:

- EMBL:   AE000520
- PIR:   F71264
- RefSeq:   NP_219369.1
- ProteinModelPortal:   O83903
- SMR:   O83903
- IntAct:   O83903
- GeneID:   2611231
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0933
- NMPDR:   fig|243276.1.peg.927
- TIGR:   TP_0933
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- ProtClustDB:   CLSK218931
- BioCyc:   TPAL243276:TP_0933-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: 6.3.2.13

Molecular weight: Translated: 59458; Mature: 59458

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: BINDING 33-33 BINDING 185-185 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYRKPITVCLASCSVQETHGSACDVSSIAYDSRAVREGSVFFALRGTHAHGAQYIHAAI
CCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHCCHHCCCCCEEEEEECCCCCCHHHHHHHH
DAGACAIVHDCPLDTYVVGVYYARVPDARCALSSAAAAFYDFPTRALTVIGVTGTEGKSS
CCCCEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCH
TVSFIAQLLRLCGKRVGFISTVEYSLGDDILPNAEHQTTPESLTVQRLLAEMREHSCEFA
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
VIEASSHGLSTRTARLQDVAFDVAVCMNVRHEHLEFHGSFEQYRFDKANVFRALDAHDHI
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCHHHH
KDGRRVPSFGVLWAEDASAVYFREATHKPCFFFKRGTGAEQRTAACLERMPCTLLWVQTL
HCCCCCCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHH
PQISQALRLRFVLSTVQEPAQPAQDGAHDVSVPLEGAFNACNIAASFLVLHGLLGTSLAA
HHHHHHHHHHHHHHHHHCCCCHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FAQHVQYVQPIQGRMQRVDMGQDFEVLIDYAHTPSSFEEILPPLAARVRARKRRMLVLFG
HHHHHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEC
SAGERDTQKRAMQGAIASRYAHVIVLTDEDPRGEDPMGILCMIAAGCEHKKLGKTLFLIP
CCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHCCEEEECC
DRVAALRHIFSLARAQDLVLLLGKGHEHSIIYAHTVQPYDEERTARELLRASLSSDTLLS
HHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLYRKPITVCLASCSVQETHGSACDVSSIAYDSRAVREGSVFFALRGTHAHGAQYIHAAI
CCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHCCHHCCCCCEEEEEECCCCCCHHHHHHHH
DAGACAIVHDCPLDTYVVGVYYARVPDARCALSSAAAAFYDFPTRALTVIGVTGTEGKSS
CCCCEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCH
TVSFIAQLLRLCGKRVGFISTVEYSLGDDILPNAEHQTTPESLTVQRLLAEMREHSCEFA
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
VIEASSHGLSTRTARLQDVAFDVAVCMNVRHEHLEFHGSFEQYRFDKANVFRALDAHDHI
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCHHHH
KDGRRVPSFGVLWAEDASAVYFREATHKPCFFFKRGTGAEQRTAACLERMPCTLLWVQTL
HCCCCCCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHH
PQISQALRLRFVLSTVQEPAQPAQDGAHDVSVPLEGAFNACNIAASFLVLHGLLGTSLAA
HHHHHHHHHHHHHHHHHCCCCHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FAQHVQYVQPIQGRMQRVDMGQDFEVLIDYAHTPSSFEEILPPLAARVRARKRRMLVLFG
HHHHHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEC
SAGERDTQKRAMQGAIASRYAHVIVLTDEDPRGEDPMGILCMIAAGCEHKKLGKTLFLIP
CCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHCCEEEECC
DRVAALRHIFSLARAQDLVLLLGKGHEHSIIYAHTVQPYDEERTARELLRASLSSDTLLS
HHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 1.5 {meso-lanthionine}} 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876