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The map label for this gene is dut

Identifier: 189026108

GI number: 189026108

Start: 963950

End: 964390

Strand: Reverse

Name: dut

Synonym: TPASS_0885

Alternate gene names: 189026108

Gene position: 964390-963950 (Counterclockwise)

Preceding gene: 189026109

Following gene: 189026107

Centisome position: 84.64

GC content: 56.24

Gene sequence:

>441_bases
ATGATCCGTGTGCGAGCTGTCGTGTATCCGGGAGCTTCTTTTCCCGAGTACCAGACGTTAGGCTCAAGTGGAGCCGATTT
GCGGGCCTTTCTTCCCGGAGGACCGCTTGAGGTTCATCCCCTGGGCCGGGTTCTCGTCCCCACGGGTGTTTGTGTGGAGC
TTCCTGTGGGGTTGGAGATGCAAATTCGTCCCCGCTCTGGGTTAGCACTCGAATACGGAGTGACGGTTTTAAATTCCCCG
GGAACGATAGACGCTGACTACCGGGGGGAAATACGCGTGTTGCTTGTCAACCTTGGGCTAGCTGCTTTCGTAGTCTCCCA
TGGAGATCGTATCGCGCAGGCGGTGATTTCTTCTGTGCTCCGTGTTGAATATGTGCGCTCCGGTAGTATCTCGCTGACTG
AACGGGGAAAAGGAGGGTACGGATCCACAGGGGTTCTGTAG

Upstream 100 bases:

>100_bases
GGATATGTGTGAAGTTGATTGATATCGACAGGATGGGTCGTTTGAATCTTTCCTATATCGATGCTCTTGAGGGGAAGTCT
GGAGGTCTGGACACGACGAA

Downstream 100 bases:

>100_bases
TTACCTAGATGTGGAGAAGAGTGCTTTTTGTGTACGTGTGCGAGGTAGTGTTCAAACACATCCTGGTGGGCGTGCTGTGC
TTTTTCTTGGTTTTTTTTGT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MIRVRAVVYPGASFPEYQTLGSSGADLRAFLPGGPLEVHPLGRVLVPTGVCVELPVGLEMQIRPRSGLALEYGVTVLNSP
GTIDADYRGEIRVLLVNLGLAAFVVSHGDRIAQAVISSVLRVEYVRSGSISLTERGKGGYGSTGVL

Sequences:

>Translated_146_residues
MIRVRAVVYPGASFPEYQTLGSSGADLRAFLPGGPLEVHPLGRVLVPTGVCVELPVGLEMQIRPRSGLALEYGVTVLNSP
GTIDADYRGEIRVLLVNLGLAAFVVSHGDRIAQAVISSVLRVEYVRSGSISLTERGKGGYGSTGVL
>Mature_146_residues
MIRVRAVVYPGASFPEYQTLGSSGADLRAFLPGGPLEVHPLGRVLVPTGVCVELPVGLEMQIRPRSGLALEYGVTVLNSP
GTIDADYRGEIRVLLVNLGLAAFVVSHGDRIAQAVISSVLRVEYVRSGSISLTERGKGGYGSTGVL

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=105, Percent_Identity=40.9523809523809, Blast_Score=81, Evalue=3e-16,
Organism=Homo sapiens, GI4503423, Length=107, Percent_Identity=40.1869158878505, Blast_Score=81, Evalue=3e-16,
Organism=Homo sapiens, GI70906441, Length=107, Percent_Identity=40.1869158878505, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1790071, Length=132, Percent_Identity=37.1212121212121, Blast_Score=88, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71988561, Length=133, Percent_Identity=42.1052631578947, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319729, Length=134, Percent_Identity=32.8358208955224, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921126, Length=124, Percent_Identity=37.9032258064516, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583610, Length=124, Percent_Identity=37.9032258064516, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_TREPA (O83855)

Other databases:

- EMBL:   AE000520
- PIR:   B71269
- RefSeq:   NP_219320.1
- ProteinModelPortal:   O83855
- SMR:   O83855
- GeneID:   2611438
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0885
- NMPDR:   fig|243276.1.peg.879
- TIGR:   TP_0885
- HOGENOM:   HBG436079
- OMA:   NEAYTIN
- ProtClustDB:   CLSK2300325
- BioCyc:   TPAL243276:TP_0885-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15376; Mature: 15376

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: BINDING 78-78

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVRAVVYPGASFPEYQTLGSSGADLRAFLPGGPLEVHPLGRVLVPTGVCVELPVGLEM
CEEEEEEEECCCCCCCHHHCCCCCCCEEEECCCCCEEECCCCEEEECCCEEEEECCCCEE
QIRPRSGLALEYGVTVLNSPGTIDADYRGEIRVLLVNLGLAAFVVSHGDRIAQAVISSVL
EEECCCCCEEEECEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHCCHHHHHHHHHHHH
RVEYVRSGSISLTERGKGGYGSTGVL
HHHEECCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MIRVRAVVYPGASFPEYQTLGSSGADLRAFLPGGPLEVHPLGRVLVPTGVCVELPVGLEM
CEEEEEEEECCCCCCCHHHCCCCCCCEEEECCCCCEEECCCCEEEECCCEEEEECCCCEE
QIRPRSGLALEYGVTVLNSPGTIDADYRGEIRVLLVNLGLAAFVVSHGDRIAQAVISSVL
EEECCCCCEEEECEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHCCHHHHHHHHHHHH
RVEYVRSGSISLTERGKGGYGSTGVL
HHHEECCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876