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The map label for this gene is pnp

Identifier: 189026109

GI number: 189026109

Start: 964387

End: 966495

Strand: Reverse

Name: pnp

Synonym: TPASS_0886

Alternate gene names: 189026109

Gene position: 966495-964387 (Counterclockwise)

Preceding gene: 189026110

Following gene: 189026108

Centisome position: 84.82

GC content: 53.53

Gene sequence:

>2109_bases
ATGAAACACAGCATAACCGGCACTATTGGGGACCACCCCCTTCTCCTTGAAAGTGGGTATCTGGCGCGGCAAGCGAACGG
GTCCGTGTATTTACAGTGTGAAGGTACGGCGATTCTCGCGACCGTATGCTCCTCTGCTCAGCGACAGGAAGGGCTCGACT
ATGTCCCGCTCACTGTTGACTTCAACGAAAAGTATTATGCGGTGGGGAAGATGCCTGGCGGCTTCATCAAGCGTGAAGGC
CGTCCGAAAGACAGAGAGATTCTCATTTCGCGCCTCATTGATCGTCCGATGCGGCCGCTCTTTGAAAAGGAGTTTGGACG
TGATATACACGTTGTCCCTACGTGCATATCGTCGGATATGGTCCATCCTCATGACGTCTTGGCTATTGTTGCCAGTTCAG
CTGCCGTTACTCTTTCGGATATCCCCTTCCATGGACCGGTTGCTGCAGTCCGCGTTGCATATCTGAACGGTTCCTATGTT
ATTAATCCTACCTTTTCTCAGATTGATGCCGCCAGTATGGAGGTTGTCGTTGCAGGCACTAGGCAGGGCATTACCATGGT
GGAAGGTGGTGCGCGAGAGGTGTCCGAGGACCTCATGCTCGGCGCGCTCGAACAGGCACAAGAGCATATTAAAGCCCTTT
GCGATATGCAGGAGCGTCTGCGTGGACTGTGTGGCAAGGAGAAACAGACGGTTATCCCTTCTTCTGCGCAGCTTGTAGGT
CGCGATCGTATTTATGAGCTCGCCTATCCGCGTCTTGCGCAGGCTTTGTATGCACAGGGGAAGGGTGAGCGGCGCAGTGC
CTGCGACGCGGTGAAGCGTGATGTTGCACAGCAGTATGCTGCTCAGCTTGAGAACGATGTGCAGCGCAGGTTGTTCGACG
CGTTGTTCCACGAGATGGAGTATGAGATACTACGGCTGAACATTCTTGACAGGGGTTTGCGCATCGATGGCCGTGCCATT
GATGCCATTCGGCCCATCGCCTGTGAGGTCGGGGTGTTGCCGCGTCCGCATGGGTCTGCGGTGTTTACCCGGGGGGAGAC
TCAATCGCTCGCGGTGGTCACGCTCGGTGCTATGTCGGACGGGCAAGTGTACGACGACATCGAAGGGGATAGACGGGAGA
ATTTTATCCTTCACTATAATTTTCCTCCTTTTTCAGTGGGGGAAATAGGGAGAATGGGGGTTGGTCGGAGGGAGATTGGG
CACGGGTGTCTAGCGCATCGTTCCCTCTCGGCAGTCATTCCGGATCCAGAACAGTTTCCTTACACCGTGCGTGTGGTTTC
AGAAATTTTGGAGTCGAATGGATCTTCCTCCATGGCGACTGTGTGCTCAGGGACGCTTTCTCTTCTCCATGCAGGGGTCC
CAATCAAAAAGCCTGTTGCGGGAATCGCGATGGGGCTCATCACCGATGGCGTGCGATATGCGATTCTTTCGGATATTTTG
GGTGAGGAGGACCATCTCGGTGATATGGATTTCAAGGTAGCAGGTACGTGTGACGGCATTACCGGTTTTCAGATGGATGT
GAAGGTAGAGGCGGTGTCTGCGTCTCTGATGAAGGAGGCTTTGCAGCAAGCTCGTGTAGGTAGGCTCCATATTCTTTCTG
TCATGAATCAGACAATCAGCGCACCGAGCGTGCACATTTCTCGCTATGCGCCGCATATTGAGAGTTTTAAAATAGCGGTT
GAAAAAATCGGCGCGCTCATCGGTCCTGGGGGGAAAACCGTTAAGTCGCTTTCTGACCAGTATCGGGTGACTATCAACAC
TGATAGTGACGGTACCGTAACCGTCTCCGGAAGGGATGCGCAGTCCGTTTTCGACGCAAAGGTCGCCGTGGTGGGCCTTA
CTGAAGATCCTCGGGTAGGGAGGGTGTATCAGGGTGTAGTGAAACGAATCGTAGAGTTCGGTGCTTTTGTGGAGATCTTT
CCAGGGAAGGAAGGACTCTGCCACGTTTCAAAGCTGTCGCGCAGCCGCGTGTCCAAAGTCAGCGATGTGCTGCAAGAGGG
TCAGCGGATATGTGTGAAGTTGATTGATATCGACAGGATGGGTCGTTTGAATCTTTCCTATATCGATGCTCTTGAGGGGA
AGTCTGGAGGTCTGGACACGACGAAATGA

Upstream 100 bases:

>100_bases
GCATAGGACGGTTGTCCGTTCTGCTTTCTTTGTCTAGCTTCGCAGGTGGCTGTGCCAGGGCTTGGTGCGGCCTGCGATAC
ATTCTGTTCTTAGGAAACGT

Downstream 100 bases:

>100_bases
TCCGTGTGCGAGCTGTCGTGTATCCGGGAGCTTCTTTTCCCGAGTACCAGACGTTAGGCTCAAGTGGAGCCGATTTGCGG
GCCTTTCTTCCCGGAGGACC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MKHSITGTIGDHPLLLESGYLARQANGSVYLQCEGTAILATVCSSAQRQEGLDYVPLTVDFNEKYYAVGKMPGGFIKREG
RPKDREILISRLIDRPMRPLFEKEFGRDIHVVPTCISSDMVHPHDVLAIVASSAAVTLSDIPFHGPVAAVRVAYLNGSYV
INPTFSQIDAASMEVVVAGTRQGITMVEGGAREVSEDLMLGALEQAQEHIKALCDMQERLRGLCGKEKQTVIPSSAQLVG
RDRIYELAYPRLAQALYAQGKGERRSACDAVKRDVAQQYAAQLENDVQRRLFDALFHEMEYEILRLNILDRGLRIDGRAI
DAIRPIACEVGVLPRPHGSAVFTRGETQSLAVVTLGAMSDGQVYDDIEGDRRENFILHYNFPPFSVGEIGRMGVGRREIG
HGCLAHRSLSAVIPDPEQFPYTVRVVSEILESNGSSSMATVCSGTLSLLHAGVPIKKPVAGIAMGLITDGVRYAILSDIL
GEEDHLGDMDFKVAGTCDGITGFQMDVKVEAVSASLMKEALQQARVGRLHILSVMNQTISAPSVHISRYAPHIESFKIAV
EKIGALIGPGGKTVKSLSDQYRVTINTDSDGTVTVSGRDAQSVFDAKVAVVGLTEDPRVGRVYQGVVKRIVEFGAFVEIF
PGKEGLCHVSKLSRSRVSKVSDVLQEGQRICVKLIDIDRMGRLNLSYIDALEGKSGGLDTTK

Sequences:

>Translated_702_residues
MKHSITGTIGDHPLLLESGYLARQANGSVYLQCEGTAILATVCSSAQRQEGLDYVPLTVDFNEKYYAVGKMPGGFIKREG
RPKDREILISRLIDRPMRPLFEKEFGRDIHVVPTCISSDMVHPHDVLAIVASSAAVTLSDIPFHGPVAAVRVAYLNGSYV
INPTFSQIDAASMEVVVAGTRQGITMVEGGAREVSEDLMLGALEQAQEHIKALCDMQERLRGLCGKEKQTVIPSSAQLVG
RDRIYELAYPRLAQALYAQGKGERRSACDAVKRDVAQQYAAQLENDVQRRLFDALFHEMEYEILRLNILDRGLRIDGRAI
DAIRPIACEVGVLPRPHGSAVFTRGETQSLAVVTLGAMSDGQVYDDIEGDRRENFILHYNFPPFSVGEIGRMGVGRREIG
HGCLAHRSLSAVIPDPEQFPYTVRVVSEILESNGSSSMATVCSGTLSLLHAGVPIKKPVAGIAMGLITDGVRYAILSDIL
GEEDHLGDMDFKVAGTCDGITGFQMDVKVEAVSASLMKEALQQARVGRLHILSVMNQTISAPSVHISRYAPHIESFKIAV
EKIGALIGPGGKTVKSLSDQYRVTINTDSDGTVTVSGRDAQSVFDAKVAVVGLTEDPRVGRVYQGVVKRIVEFGAFVEIF
PGKEGLCHVSKLSRSRVSKVSDVLQEGQRICVKLIDIDRMGRLNLSYIDALEGKSGGLDTTK
>Mature_702_residues
MKHSITGTIGDHPLLLESGYLARQANGSVYLQCEGTAILATVCSSAQRQEGLDYVPLTVDFNEKYYAVGKMPGGFIKREG
RPKDREILISRLIDRPMRPLFEKEFGRDIHVVPTCISSDMVHPHDVLAIVASSAAVTLSDIPFHGPVAAVRVAYLNGSYV
INPTFSQIDAASMEVVVAGTRQGITMVEGGAREVSEDLMLGALEQAQEHIKALCDMQERLRGLCGKEKQTVIPSSAQLVG
RDRIYELAYPRLAQALYAQGKGERRSACDAVKRDVAQQYAAQLENDVQRRLFDALFHEMEYEILRLNILDRGLRIDGRAI
DAIRPIACEVGVLPRPHGSAVFTRGETQSLAVVTLGAMSDGQVYDDIEGDRRENFILHYNFPPFSVGEIGRMGVGRREIG
HGCLAHRSLSAVIPDPEQFPYTVRVVSEILESNGSSSMATVCSGTLSLLHAGVPIKKPVAGIAMGLITDGVRYAILSDIL
GEEDHLGDMDFKVAGTCDGITGFQMDVKVEAVSASLMKEALQQARVGRLHILSVMNQTISAPSVHISRYAPHIESFKIAV
EKIGALIGPGGKTVKSLSDQYRVTINTDSDGTVTVSGRDAQSVFDAKVAVVGLTEDPRVGRVYQGVVKRIVEFGAFVEIF
PGKEGLCHVSKLSRSRVSKVSDVLQEGQRICVKLIDIDRMGRLNLSYIDALEGKSGGLDTTK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=704, Percent_Identity=35.3693181818182, Blast_Score=421, Evalue=1e-118,
Organism=Escherichia coli, GI145693187, Length=685, Percent_Identity=46.8613138686131, Blast_Score=623, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI115534063, Length=647, Percent_Identity=34.1576506955178, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI281362905, Length=690, Percent_Identity=35.6521739130435, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24651641, Length=690, Percent_Identity=35.6521739130435, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24651643, Length=690, Percent_Identity=35.6521739130435, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI161079377, Length=638, Percent_Identity=35.423197492163, Blast_Score=377, Evalue=1e-104,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_TREPA (O83856)

Other databases:

- EMBL:   AE000520
- PIR:   C71269
- RefSeq:   NP_219321.1
- HSSP:   P05055
- ProteinModelPortal:   O83856
- GeneID:   2611780
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0886
- NMPDR:   fig|243276.1.peg.880
- TIGR:   TP_0886
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   TPAL243276:TP_0886-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76334; Mature: 76334

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSITGTIGDHPLLLESGYLARQANGSVYLQCEGTAILATVCSSAQRQEGLDYVPLTVD
CCCCEECCCCCCCEEEECCCEEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEE
FNEKYYAVGKMPGGFIKREGRPKDREILISRLIDRPMRPLFEKEFGRDIHVVPTCISSDM
CCCCEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCEEEEEEEHHCCCC
VHPHDVLAIVASSAAVTLSDIPFHGPVAAVRVAYLNGSYVINPTFSQIDAASMEVVVAGT
CCHHHHHHHHHCCCEEEEECCCCCCCHHHEEEEEECCCEEECCCHHHCCCCCEEEEEECC
RQGITMVEGGAREVSEDLMLGALEQAQEHIKALCDMQERLRGLCGKEKQTVIPSSAQLVG
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHH
RDRIYELAYPRLAQALYAQGKGERRSACDAVKRDVAQQYAAQLENDVQRRLFDALFHEME
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEILRLNILDRGLRIDGRAIDAIRPIACEVGVLPRPHGSAVFTRGETQSLAVVTLGAMSD
HHHEEEEECCCCCEECCEECCCCCCHHHCCCCCCCCCCCEEEECCCCCEEEEEEEECCCC
GQVYDDIEGDRRENFILHYNFPPFSVGEIGRMGVGRREIGHGCLAHRSLSAVIPDPEQFP
CCEECCCCCCCCCCEEEEECCCCCCCCCHHHCCCCHHHHCCHHHHHCCCCCCCCCCCCCC
YTVRVVSEILESNGSSSMATVCSGTLSLLHAGVPIKKPVAGIAMGLITDGVRYAILSDIL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
GEEDHLGDMDFKVAGTCDGITGFQMDVKVEAVSASLMKEALQQARVGRLHILSVMNQTIS
CCCCCCCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
APSVHISRYAPHIESFKIAVEKIGALIGPGGKTVKSLSDQYRVTINTDSDGTVTVSGRDA
CCCEEHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCCCEEEECCCCH
QSVFDAKVAVVGLTEDPRVGRVYQGVVKRIVEFGAFVEIFPGKEGLCHVSKLSRSRVSKV
HHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
SDVLQEGQRICVKLIDIDRMGRLNLSYIDALEGKSGGLDTTK
HHHHHHHHHHEEHEECCCCCCCCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKHSITGTIGDHPLLLESGYLARQANGSVYLQCEGTAILATVCSSAQRQEGLDYVPLTVD
CCCCEECCCCCCCEEEECCCEEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEE
FNEKYYAVGKMPGGFIKREGRPKDREILISRLIDRPMRPLFEKEFGRDIHVVPTCISSDM
CCCCEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCEEEEEEEHHCCCC
VHPHDVLAIVASSAAVTLSDIPFHGPVAAVRVAYLNGSYVINPTFSQIDAASMEVVVAGT
CCHHHHHHHHHCCCEEEEECCCCCCCHHHEEEEEECCCEEECCCHHHCCCCCEEEEEECC
RQGITMVEGGAREVSEDLMLGALEQAQEHIKALCDMQERLRGLCGKEKQTVIPSSAQLVG
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHH
RDRIYELAYPRLAQALYAQGKGERRSACDAVKRDVAQQYAAQLENDVQRRLFDALFHEME
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEILRLNILDRGLRIDGRAIDAIRPIACEVGVLPRPHGSAVFTRGETQSLAVVTLGAMSD
HHHEEEEECCCCCEECCEECCCCCCHHHCCCCCCCCCCCEEEECCCCCEEEEEEEECCCC
GQVYDDIEGDRRENFILHYNFPPFSVGEIGRMGVGRREIGHGCLAHRSLSAVIPDPEQFP
CCEECCCCCCCCCCEEEEECCCCCCCCCHHHCCCCHHHHCCHHHHHCCCCCCCCCCCCCC
YTVRVVSEILESNGSSSMATVCSGTLSLLHAGVPIKKPVAGIAMGLITDGVRYAILSDIL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
GEEDHLGDMDFKVAGTCDGITGFQMDVKVEAVSASLMKEALQQARVGRLHILSVMNQTIS
CCCCCCCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
APSVHISRYAPHIESFKIAVEKIGALIGPGGKTVKSLSDQYRVTINTDSDGTVTVSGRDA
CCCEEHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCCCEEEECCCCH
QSVFDAKVAVVGLTEDPRVGRVYQGVVKRIVEFGAFVEIFPGKEGLCHVSKLSRSRVSKV
HHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
SDVLQEGQRICVKLIDIDRMGRLNLSYIDALEGKSGGLDTTK
HHHHHHHHHHEEHEECCCCCCCCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876