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The map label for this gene is rph [H]

Identifier: 188586564

GI number: 188586564

Start: 2059593

End: 2060333

Strand: Reverse

Name: rph [H]

Synonym: Nther_1951

Alternate gene names: 188586564

Gene position: 2060333-2059593 (Counterclockwise)

Preceding gene: 188586565

Following gene: 188586563

Centisome position: 65.09

GC content: 38.33

Gene sequence:

>741_bases
ATGAGAGAAAACAGAAAAGTTGACGAAATTCGTCCTGTTAAAATTGAAACTGATTTTGTCAAACATCCAGAGGGATCGGT
ATTAATAACAGTGGGAGATACCAAGGTCATCTGTAGTGCGACTATTGAGTCCAAGGTTCCATCCTTTTTAAAAGGTGAAG
GAAAAGGTTGGGTGACAGCCGAATACTCTATGTTACCTCGTGCCACAGAGTCGAGAAATATTAGAGAAGCGGCAAAAGGA
AAGCTGGGAGGTCGTACCCAGGAAATTCAAAGATTAATTGGCAGGTCTTTGAGATCTATGGTAAATCTCAAAAAACTAGG
AGAACGGACAATATGGTTAGACTGTGATGTAATTCAAGCCGATGGAGGTACTAGAACAGCTTCTATAACAGGTGCTTTTG
TGTCTATGGTGTTAGCATTAAACAAACTAGTGGAAGAGAAAGAATTGTCCGAGTTACCTGTAGAAGGTTATTTAGCTGCT
ATTAGTGTAGGAATAGTAAATGATGAAATAATGCTGGATTTAGATGGTAAGGAAGACTTTCAAGCTGATACAGATATGAA
TGTGGCAGGGACTGATAAAGGTGAATTTGTAGAAATTCAAGGTACTGGTGAAAATCATCCTTTTAGCAAAAATGAAATGG
AAAAAATGTTAAGCTATGCTGAGAAAGGAATGCATGCTTTAATGGAAGTACAAAGTCAAGCTTTATCAGAAATCTATCCA
CCTCCTGCCAAGAAAGGATGA

Upstream 100 bases:

>100_bases
TTAATCGGCAGAGTAGGGGAGGCAAGGGGATCATTACTGTCTTTTAATTTGGAATAGTTTTTAAGGACTTTAAGGACATA
AAAAACAAAGGAGTGACACT

Downstream 100 bases:

>100_bases
TTATCATGAAACTTGTACTCGCCTCAGGTAATCAGGGAAAACTTAACGAATTAAAAGCATTACTTAGCAAACAGCCAGTT
GAAATTTATTCTATGTCGGA

Product: RNAse PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MRENRKVDEIRPVKIETDFVKHPEGSVLITVGDTKVICSATIESKVPSFLKGEGKGWVTAEYSMLPRATESRNIREAAKG
KLGGRTQEIQRLIGRSLRSMVNLKKLGERTIWLDCDVIQADGGTRTASITGAFVSMVLALNKLVEEKELSELPVEGYLAA
ISVGIVNDEIMLDLDGKEDFQADTDMNVAGTDKGEFVEIQGTGENHPFSKNEMEKMLSYAEKGMHALMEVQSQALSEIYP
PPAKKG

Sequences:

>Translated_246_residues
MRENRKVDEIRPVKIETDFVKHPEGSVLITVGDTKVICSATIESKVPSFLKGEGKGWVTAEYSMLPRATESRNIREAAKG
KLGGRTQEIQRLIGRSLRSMVNLKKLGERTIWLDCDVIQADGGTRTASITGAFVSMVLALNKLVEEKELSELPVEGYLAA
ISVGIVNDEIMLDLDGKEDFQADTDMNVAGTDKGEFVEIQGTGENHPFSKNEMEKMLSYAEKGMHALMEVQSQALSEIYP
PPAKKG
>Mature_246_residues
MRENRKVDEIRPVKIETDFVKHPEGSVLITVGDTKVICSATIESKVPSFLKGEGKGWVTAEYSMLPRATESRNIREAAKG
KLGGRTQEIQRLIGRSLRSMVNLKKLGERTIWLDCDVIQADGGTRTASITGAFVSMVLALNKLVEEKELSELPVEGYLAA
ISVGIVNDEIMLDLDGKEDFQADTDMNVAGTDKGEFVEIQGTGENHPFSKNEMEKMLSYAEKGMHALMEVQSQALSEIYP
PPAKKG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=205, Percent_Identity=61.4634146341463, Blast_Score=263, Evalue=8e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27060; Mature: 27060

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRENRKVDEIRPVKIETDFVKHPEGSVLITVGDTKVICSATIESKVPSFLKGEGKGWVTA
CCCCCCCCCCCCEEEECCHHCCCCCCEEEEECCCEEEEEECHHHHCCHHHCCCCCCEEEE
EYSMLPRATESRNIREAAKGKLGGRTQEIQRLIGRSLRSMVNLKKLGERTIWLDCDVIQA
EHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEC
DGGTRTASITGAFVSMVLALNKLVEEKELSELPVEGYLAAISVGIVNDEIMLDLDGKEDF
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHEEEEEECCEEEEEECCCCCC
QADTDMNVAGTDKGEFVEIQGTGENHPFSKNEMEKMLSYAEKGMHALMEVQSQALSEIYP
CCCCCCCEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PPAKKG
CCCCCC
>Mature Secondary Structure
MRENRKVDEIRPVKIETDFVKHPEGSVLITVGDTKVICSATIESKVPSFLKGEGKGWVTA
CCCCCCCCCCCCEEEECCHHCCCCCCEEEEECCCEEEEEECHHHHCCHHHCCCCCCEEEE
EYSMLPRATESRNIREAAKGKLGGRTQEIQRLIGRSLRSMVNLKKLGERTIWLDCDVIQA
EHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEC
DGGTRTASITGAFVSMVLALNKLVEEKELSELPVEGYLAAISVGIVNDEIMLDLDGKEDF
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHEEEEEECCEEEEEECCCCCC
QADTDMNVAGTDKGEFVEIQGTGENHPFSKNEMEKMLSYAEKGMHALMEVQSQALSEIYP
CCCCCCCEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PPAKKG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA