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The map label for this gene is ilvB [H]

Identifier: 188586546

GI number: 188586546

Start: 2032145

End: 2033809

Strand: Reverse

Name: ilvB [H]

Synonym: Nther_1932

Alternate gene names: 188586546

Gene position: 2033809-2032145 (Counterclockwise)

Preceding gene: 188586547

Following gene: 188586545

Centisome position: 64.25

GC content: 36.94

Gene sequence:

>1665_bases
TTGACACTTAAAGTTTCACAAGTAATAGTCAATTGTTTGGAGAATTTAGGCATAGAACATGTTTTTGGTATCCCTGGAAT
ACATAATTTAGATATTTATGAACAACTGATCAGAAGTGAAATACAGCATATTACCGCTCGGCATGAAGAAGGTGCAGGTT
ACATGGCAGATGGTTATGCCCGGGTTTCTGGTGATCCAACTGTTTGTTTTGTTATTACTGGACCTGGCCTTACCAATATT
TCAACTGCTGTAGCACAAGCTTATTCTGATTCTGTGCCAATGATAATCATTTCCACTCAGGTACCGGATAGATTCCGGGG
AAAAAATAAGGGTTTTTTACATGAATTGAATAACTCTACCAATTTCATGAGATCTTTTGTCAAAGAAAGTAGACAAATCA
ATAGAAAGGAATATGTAGCAATAGCTTTGAGAGAAGCCTACCAACTTTCTATTTCAGGTAGGGGTGGACCTGTGCATCTT
GAAATACCTATAGATATTTTACAGTCAGAGTTTGAGTATTATCCGGAACCTCTCCATTTTACACGATACCCAGTGCAAGA
ACCTTGGACTTGTAATCCGGACAAAGATTCTTTGGAGGAGGCCGTGAATCTGATTGAAAACTCTGATAGTCCAGTAATTA
TTGCAGGAGGGGGGAGTGCAAATGGGAGGGATCTGGTTATTGATCTTGCAGAGAGATTGCAAGTACCCGTTTTACAAACA
GTTAGTGCTAAAGGGATAATTCCAGAAGATCATCCTCTATGTATAGGAACAAGGCTTCACCTCCCTTCAGCAGCTGAGAT
GTTAAGGGAAAGTGATTTGGTAATAGCCATTGGTTCAGATCTGTCCCTAGCAGACTTTTGGGACCGAGAAATATTTTTCA
ATAATTTAATACAAATCAATATTGACTCTAGCAGTTATTATGAAAAATGCTATCGTAATGTTTTTTTATACGGAGATGTT
AGGTGTATTGTGGACTCCATTTTATCTAAATTAAAAAGCAGTCATCCTTGGGAATATAAAGAATTTAATGAAAAAGAGGC
TAGAAGTAGATCATTAACACAAAGTGTAGTTTCAAAAACCAAAGCTGAATTAGAGGAAGTTACAGGGATAAAAAATAATT
TAAATCAATTATTAGAGCTAATAAATGTGTTAAGGCTTGAACTGCCGAGTACTGGTATTTTAGTGACTGATTTAACTACT
CCATCTTATCTAGCAATAAGTGAATTTCCTGCATACTATACATCGACCTTTTTGCATCCAGTTAATTTTGGTACTTTGGG
ATATGCACTGCCAGCAGCAATAGGAGCTTACTTGAGTCTCGGCTGTAAAACAGTGGTTTTAACTGGTGATGGGAGTATTC
AATTTACTATGCAAGAATTAGCTGTAATAGCTGATTTACAGCTCCCCATAGTAATTGTGGTTTGGAATGATCAAGGTTAT
GGTGAGATTAGGCGCAATCAAAAAGCTCGTCAATTTACTTCTACAATTGCCGTTGACCATACTCCTCCGTCCTTTGAATT
TTTAGCACAGGCTTATAACTTACAATTTTATAATATTACAGAGGAGCAAGAGTCTGAAAAAATTAAAGGTATAATTCAAT
GGGCACTGAGTTCTAATAAACCCTCGTTAATAGAAATAACTCCGGGTGGTGGTGTAGATGCTTAA

Upstream 100 bases:

>100_bases
ATATCTGGCAGGTTCATGCCAGAGGAATGGTCACTTGACTTATATATTATCTATGGTATAGGAAAATGGAAATTAATTTT
AATCTTAGAGGTGATAAAAG

Downstream 100 bases:

>100_bases
AAGTTTTAGAAAACCCTTGCATAATATGGAACCACATTATATCTCGGAAAATACATTGGAAAAAAAGCGTCGTGAGTTAG
GTCTATCTCACTTTGTTAAA

Product: thiamine pyrophosphate protein TPP binding domain protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MTLKVSQVIVNCLENLGIEHVFGIPGIHNLDIYEQLIRSEIQHITARHEEGAGYMADGYARVSGDPTVCFVITGPGLTNI
STAVAQAYSDSVPMIIISTQVPDRFRGKNKGFLHELNNSTNFMRSFVKESRQINRKEYVAIALREAYQLSISGRGGPVHL
EIPIDILQSEFEYYPEPLHFTRYPVQEPWTCNPDKDSLEEAVNLIENSDSPVIIAGGGSANGRDLVIDLAERLQVPVLQT
VSAKGIIPEDHPLCIGTRLHLPSAAEMLRESDLVIAIGSDLSLADFWDREIFFNNLIQINIDSSSYYEKCYRNVFLYGDV
RCIVDSILSKLKSSHPWEYKEFNEKEARSRSLTQSVVSKTKAELEEVTGIKNNLNQLLELINVLRLELPSTGILVTDLTT
PSYLAISEFPAYYTSTFLHPVNFGTLGYALPAAIGAYLSLGCKTVVLTGDGSIQFTMQELAVIADLQLPIVIVVWNDQGY
GEIRRNQKARQFTSTIAVDHTPPSFEFLAQAYNLQFYNITEEQESEKIKGIIQWALSSNKPSLIEITPGGGVDA

Sequences:

>Translated_554_residues
MTLKVSQVIVNCLENLGIEHVFGIPGIHNLDIYEQLIRSEIQHITARHEEGAGYMADGYARVSGDPTVCFVITGPGLTNI
STAVAQAYSDSVPMIIISTQVPDRFRGKNKGFLHELNNSTNFMRSFVKESRQINRKEYVAIALREAYQLSISGRGGPVHL
EIPIDILQSEFEYYPEPLHFTRYPVQEPWTCNPDKDSLEEAVNLIENSDSPVIIAGGGSANGRDLVIDLAERLQVPVLQT
VSAKGIIPEDHPLCIGTRLHLPSAAEMLRESDLVIAIGSDLSLADFWDREIFFNNLIQINIDSSSYYEKCYRNVFLYGDV
RCIVDSILSKLKSSHPWEYKEFNEKEARSRSLTQSVVSKTKAELEEVTGIKNNLNQLLELINVLRLELPSTGILVTDLTT
PSYLAISEFPAYYTSTFLHPVNFGTLGYALPAAIGAYLSLGCKTVVLTGDGSIQFTMQELAVIADLQLPIVIVVWNDQGY
GEIRRNQKARQFTSTIAVDHTPPSFEFLAQAYNLQFYNITEEQESEKIKGIIQWALSSNKPSLIEITPGGGVDA
>Mature_553_residues
TLKVSQVIVNCLENLGIEHVFGIPGIHNLDIYEQLIRSEIQHITARHEEGAGYMADGYARVSGDPTVCFVITGPGLTNIS
TAVAQAYSDSVPMIIISTQVPDRFRGKNKGFLHELNNSTNFMRSFVKESRQINRKEYVAIALREAYQLSISGRGGPVHLE
IPIDILQSEFEYYPEPLHFTRYPVQEPWTCNPDKDSLEEAVNLIENSDSPVIIAGGGSANGRDLVIDLAERLQVPVLQTV
SAKGIIPEDHPLCIGTRLHLPSAAEMLRESDLVIAIGSDLSLADFWDREIFFNNLIQINIDSSSYYEKCYRNVFLYGDVR
CIVDSILSKLKSSHPWEYKEFNEKEARSRSLTQSVVSKTKAELEEVTGIKNNLNQLLELINVLRLELPSTGILVTDLTTP
SYLAISEFPAYYTSTFLHPVNFGTLGYALPAAIGAYLSLGCKTVVLTGDGSIQFTMQELAVIADLQLPIVIVVWNDQGYG
EIRRNQKARQFTSTIAVDHTPPSFEFLAQAYNLQFYNITEEQESEKIKGIIQWALSSNKPSLIEITPGGGVDA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=23.0366492146597, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI21361361, Length=569, Percent_Identity=22.3198594024605, Blast_Score=109, Evalue=7e-24,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=28.7795992714025, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI87081685, Length=555, Percent_Identity=28.4684684684685, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1786717, Length=499, Percent_Identity=28.8577154308617, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1788716, Length=562, Percent_Identity=24.9110320284698, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1787096, Length=566, Percent_Identity=21.7314487632509, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17542570, Length=584, Percent_Identity=25.3424657534247, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=508, Percent_Identity=23.0314960629921, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17531301, Length=508, Percent_Identity=23.0314960629921, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=580, Percent_Identity=25.3448275862069, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320816, Length=513, Percent_Identity=24.1715399610136, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6323073, Length=502, Percent_Identity=23.3067729083665, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=508, Percent_Identity=24.4094488188976, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6321524, Length=488, Percent_Identity=22.7459016393443, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320588, Length=552, Percent_Identity=21.3768115942029, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=22.8070175438596, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61642; Mature: 61511

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKVSQVIVNCLENLGIEHVFGIPGIHNLDIYEQLIRSEIQHITARHEEGAGYMADGYA
CCEEHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCEE
RVSGDPTVCFVITGPGLTNISTAVAQAYSDSVPMIIISTQVPDRFRGKNKGFLHELNNST
EECCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCHHHHCCCCH
NFMRSFVKESRQINRKEYVAIALREAYQLSISGRGGPVHLEIPIDILQSEFEYYPEPLHF
HHHHHHHHHHHHCCHHHHHHEEEEEEEEEEEECCCCEEEEEECHHHHHHHHHHCCCCCCC
TRYPVQEPWTCNPDKDSLEEAVNLIENSDSPVIIAGGGSANGRDLVIDLAERLQVPVLQT
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHEEHHHHCCCCHHHH
VSAKGIIPEDHPLCIGTRLHLPSAAEMLRESDLVIAIGSDLSLADFWDREIFFNNLIQIN
CCCCCCCCCCCCEEEEEEECCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEE
IDSSSYYEKCYRNVFLYGDVRCIVDSILSKLKSSHPWEYKEFNEKEARSRSLTQSVVSKT
ECCHHHHHHHHHCEEEECHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHH
KAELEEVTGIKNNLNQLLELINVLRLELPSTGILVTDLTTPSYLAISEFPAYYTSTFLHP
HHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHCCHHHHHHHCCC
VNFGTLGYALPAAIGAYLSLGCKTVVLTGDGSIQFTMQELAVIADLQLPIVIVVWNDQGY
CCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHEECCCCEEEEEECCCCC
GEIRRNQKARQFTSTIAVDHTPPSFEFLAQAYNLQFYNITEEQESEKIKGIIQWALSSNK
HHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHCCCC
PSLIEITPGGGVDA
CCEEEECCCCCCCC
>Mature Secondary Structure 
TLKVSQVIVNCLENLGIEHVFGIPGIHNLDIYEQLIRSEIQHITARHEEGAGYMADGYA
CEEHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCEE
RVSGDPTVCFVITGPGLTNISTAVAQAYSDSVPMIIISTQVPDRFRGKNKGFLHELNNST
EECCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCHHHHCCCCH
NFMRSFVKESRQINRKEYVAIALREAYQLSISGRGGPVHLEIPIDILQSEFEYYPEPLHF
HHHHHHHHHHHHCCHHHHHHEEEEEEEEEEEECCCCEEEEEECHHHHHHHHHHCCCCCCC
TRYPVQEPWTCNPDKDSLEEAVNLIENSDSPVIIAGGGSANGRDLVIDLAERLQVPVLQT
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHEEHHHHCCCCHHHH
VSAKGIIPEDHPLCIGTRLHLPSAAEMLRESDLVIAIGSDLSLADFWDREIFFNNLIQIN
CCCCCCCCCCCCEEEEEEECCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEE
IDSSSYYEKCYRNVFLYGDVRCIVDSILSKLKSSHPWEYKEFNEKEARSRSLTQSVVSKT
ECCHHHHHHHHHCEEEECHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHH
KAELEEVTGIKNNLNQLLELINVLRLELPSTGILVTDLTTPSYLAISEFPAYYTSTFLHP
HHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHCCHHHHHHHCCC
VNFGTLGYALPAAIGAYLSLGCKTVVLTGDGSIQFTMQELAVIADLQLPIVIVVWNDQGY
CCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHEECCCCEEEEEECCCCC
GEIRRNQKARQFTSTIAVDHTPPSFEFLAQAYNLQFYNITEEQESEKIKGIIQWALSSNK
HHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHCCCC
PSLIEITPGGGVDA
CCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]