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The map label for this gene is pmbA [H]

Identifier: 188586460

GI number: 188586460

Start: 1922770

End: 1924056

Strand: Reverse

Name: pmbA [H]

Synonym: Nther_1845

Alternate gene names: 188586460

Gene position: 1924056-1922770 (Counterclockwise)

Preceding gene: 188586461

Following gene: 188586459

Centisome position: 60.78

GC content: 32.87

Gene sequence:

>1287_bases
ATGGAACTATTAAATAAATGTTTGACTAATTTAAAAGAAAAGGGTGCCGATAAATCTGAAGTATATTTAACAAAAGAAAT
TAAACAAGAGATGAATATCCACTCCGGTGAGATTAAGCTTTTGAGGACCTTAGAAGAATATAATCTTGAAATGAGCGCTA
TCATTGATGATAAAAAAGATACAATTAAATTGAGTGAGATAGATGATCAATCTATCGAAGAAGCTGCCAGTGAAGTAATC
GAAAATGCCAAGAATTCTCAGCCCGATGAAGCTAATGATATTTCTGAAAATGCACTACAAAAAGAATTTCAAGATGACAA
AACTGATGTTGATATAGAGCTGATGCATGAAAGGTTACATAATTTTATCCAGAAAGTTGACCAGGAGTTCCCTGATGTGA
TTATTGAAGAAGCTATTCTAGTATATAATGATAGATATGATTATTATTCCAATTCTAATGGTGTAAATTTGACATCGAAT
TCAGACAATTATTCGTTCTTTGTCATGTTTTTTGCTAAAAAAGAGCAAAAGACCTCTTCTTTTAATTATACATTCACATG
TACAAAAGATTTGGATAAAGAAATACTCGAGCTAGGGTCTCTGTCTTATTTGTTAGAACAATCCATTGAACATTTAGAAC
CAAAAAACCTTGAAAACAAAAAAATCACGGGTGATGTGATTATTACTCCTGATTGTATGAACGAATTGCTATCTTTCGTG
CTTGCTCACTTGCAAAACCATTATTTGATTTCAGGGATTTCAAAGTTCCAGGACAGGATTGATGAAAAAGTTATTGACGA
TAAATTTACATTGAGAACCGAACCTGACTCTGGAGTATTGGCCGACAAAAACTATTTTGGTAAAGATGGTATAGTAAATG
AAAATGACTACATTTTTGAAAATGGAGTACTGAAAAACTATTTGCTAGATTTATACGGTTCAAAGAAAACAGGTTTTGAT
AGAGGTCCTTCTACAGGAGTTAACTTGGTTATGGAAAATGGGGAAAGAAGTTTTGAGGAAATGATTGAATCTGTAGATCA
GGGTATTGTTTTATCGAGATTTTCAGGTGGCGCTCCTGCACCAAATGGAGATTTTTCGGGTGTGGCAAAGAATAGTTATT
ATATTGAAGACGGGAAAATTAAATATCCAATTAAAGAGACAATGGTTTCCGGAAATCTTTTCGATATGTTACAGAATATC
AAGGGAATTTCCAAGGAAAGGATTAACAATGGCGAGTCTTTACTGCCTTATGTTCAATTCCAGAATGTTACCATTTCATC
TAAATAG

Upstream 100 bases:

>100_bases
ACATGGTCAAATGCTGGGATGTGTGGAAAAAAACAAGTGATACCAGTTGGAATGGGCGGACCTGCTATAAAGTGTAAAAT
TAATATAGGAGGTAGGTAAT

Downstream 100 bases:

>100_bases
ATTTTGTATTGTCAATTGGGCTGTGATAAAGTTGAATATTGATAGTGTAAATTAATTTCAAATTAAAAAATAAAAGTAAA
AAAGTCGTAAACAATAATCA

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MELLNKCLTNLKEKGADKSEVYLTKEIKQEMNIHSGEIKLLRTLEEYNLEMSAIIDDKKDTIKLSEIDDQSIEEAASEVI
ENAKNSQPDEANDISENALQKEFQDDKTDVDIELMHERLHNFIQKVDQEFPDVIIEEAILVYNDRYDYYSNSNGVNLTSN
SDNYSFFVMFFAKKEQKTSSFNYTFTCTKDLDKEILELGSLSYLLEQSIEHLEPKNLENKKITGDVIITPDCMNELLSFV
LAHLQNHYLISGISKFQDRIDEKVIDDKFTLRTEPDSGVLADKNYFGKDGIVNENDYIFENGVLKNYLLDLYGSKKTGFD
RGPSTGVNLVMENGERSFEEMIESVDQGIVLSRFSGGAPAPNGDFSGVAKNSYYIEDGKIKYPIKETMVSGNLFDMLQNI
KGISKERINNGESLLPYVQFQNVTISSK

Sequences:

>Translated_428_residues
MELLNKCLTNLKEKGADKSEVYLTKEIKQEMNIHSGEIKLLRTLEEYNLEMSAIIDDKKDTIKLSEIDDQSIEEAASEVI
ENAKNSQPDEANDISENALQKEFQDDKTDVDIELMHERLHNFIQKVDQEFPDVIIEEAILVYNDRYDYYSNSNGVNLTSN
SDNYSFFVMFFAKKEQKTSSFNYTFTCTKDLDKEILELGSLSYLLEQSIEHLEPKNLENKKITGDVIITPDCMNELLSFV
LAHLQNHYLISGISKFQDRIDEKVIDDKFTLRTEPDSGVLADKNYFGKDGIVNENDYIFENGVLKNYLLDLYGSKKTGFD
RGPSTGVNLVMENGERSFEEMIESVDQGIVLSRFSGGAPAPNGDFSGVAKNSYYIEDGKIKYPIKETMVSGNLFDMLQNI
KGISKERINNGESLLPYVQFQNVTISSK
>Mature_428_residues
MELLNKCLTNLKEKGADKSEVYLTKEIKQEMNIHSGEIKLLRTLEEYNLEMSAIIDDKKDTIKLSEIDDQSIEEAASEVI
ENAKNSQPDEANDISENALQKEFQDDKTDVDIELMHERLHNFIQKVDQEFPDVIIEEAILVYNDRYDYYSNSNGVNLTSN
SDNYSFFVMFFAKKEQKTSSFNYTFTCTKDLDKEILELGSLSYLLEQSIEHLEPKNLENKKITGDVIITPDCMNELLSFV
LAHLQNHYLISGISKFQDRIDEKVIDDKFTLRTEPDSGVLADKNYFGKDGIVNENDYIFENGVLKNYLLDLYGSKKTGFD
RGPSTGVNLVMENGERSFEEMIESVDQGIVLSRFSGGAPAPNGDFSGVAKNSYYIEDGKIKYPIKETMVSGNLFDMLQNI
KGISKERINNGESLLPYVQFQNVTISSK

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=428, Percent_Identity=21.9626168224299, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48744; Mature: 48744

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLNKCLTNLKEKGADKSEVYLTKEIKQEMNIHSGEIKLLRTLEEYNLEMSAIIDDKKD
CHHHHHHHHHHHHCCCCHHHEEEHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCCC
TIKLSEIDDQSIEEAASEVIENAKNSQPDEANDISENALQKEFQDDKTDVDIELMHERLH
EEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
NFIQKVDQEFPDVIIEEAILVYNDRYDYYSNSNGVNLTSNSDNYSFFVMFFAKKEQKTSS
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCC
FNYTFTCTKDLDKEILELGSLSYLLEQSIEHLEPKNLENKKITGDVIITPDCMNELLSFV
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEECHHHHHHHHHHH
LAHLQNHYLISGISKFQDRIDEKVIDDKFTLRTEPDSGVLADKNYFGKDGIVNENDYIFE
HHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCCCCCCCCCEEEE
NGVLKNYLLDLYGSKKTGFDRGPSTGVNLVMENGERSFEEMIESVDQGIVLSRFSGGAPA
CCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCC
PNGDFSGVAKNSYYIEDGKIKYPIKETMVSGNLFDMLQNIKGISKERINNGESLLPYVQF
CCCCCCCEECCCEEEECCEEECCHHHHHHCCHHHHHHHHHHCCCHHHCCCCCCCCCEEEE
QNVTISSK
ECEEECCC
>Mature Secondary Structure
MELLNKCLTNLKEKGADKSEVYLTKEIKQEMNIHSGEIKLLRTLEEYNLEMSAIIDDKKD
CHHHHHHHHHHHHCCCCHHHEEEHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCCC
TIKLSEIDDQSIEEAASEVIENAKNSQPDEANDISENALQKEFQDDKTDVDIELMHERLH
EEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
NFIQKVDQEFPDVIIEEAILVYNDRYDYYSNSNGVNLTSNSDNYSFFVMFFAKKEQKTSS
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCC
FNYTFTCTKDLDKEILELGSLSYLLEQSIEHLEPKNLENKKITGDVIITPDCMNELLSFV
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEECHHHHHHHHHHH
LAHLQNHYLISGISKFQDRIDEKVIDDKFTLRTEPDSGVLADKNYFGKDGIVNENDYIFE
HHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCCCCCCCCCEEEE
NGVLKNYLLDLYGSKKTGFDRGPSTGVNLVMENGERSFEEMIESVDQGIVLSRFSGGAPA
CCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCC
PNGDFSGVAKNSYYIEDGKIKYPIKETMVSGNLFDMLQNIKGISKERINNGESLLPYVQF
CCCCCCCEECCCEEEECCEEECCHHHHHHCCHHHHHHHHHHCCCHHHCCCCCCCCCEEEE
QNVTISSK
ECEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]