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The map label for this gene is punA [H]

Identifier: 188586283

GI number: 188586283

Start: 1742711

End: 1743526

Strand: Reverse

Name: punA [H]

Synonym: Nther_1666

Alternate gene names: 188586283

Gene position: 1743526-1742711 (Counterclockwise)

Preceding gene: 188586284

Following gene: 188586282

Centisome position: 55.08

GC content: 38.6

Gene sequence:

>816_bases
ATGGATTTTCATAAAATAACAGAAGCTAAGGAGTATATATTGAATCAGTGTGATGAAGTTCCATCAATTGGTTTGATTTT
AGGGTCAGGTTTGGGAAGTTTAGCTGACGAAATTGAAAATGTTAACTATATACCATACCAAGATATCCCTCATTTTCCCG
AATCTACAGTTAAAGGGCATAAGGGGAGGTTAGCAATAGGTAAACTTGAGAGCAAAACGGTCATGGCTATGCAGGGTAGA
TTTCATTACTATGAAGGATATGATTTGAAAGAAGTGACATTTCCAGTTCGTGTAATGCAAAATATGGGAATAGACAAACT
GATAGTAACAAACGCAGCAGGTGGAATTGATTTGAATTTTTCAGTGGGAGGTTTGATGTTAATTACCGATCATATTAACT
TTTTTGGCGCAAATCCCTTGAGAGGAAAAAATGATGATCGGCTAGGAACCCGCTTCCCAGATATGACCTATGCCTATGAC
CCTGAATTACGAGAAGTAGCTAAACAAGCCGCCAATGAACTGGACATAGGACTTTATGAAGGCGTATATTTAGGAGTACC
TGGTCCCTCTTATGAAACTCCTGCTGAGATCAAAATGGCTCGAACCATAGGAGCAAGTGCAGTGGGTATGTCTACAGTGC
CAGAAGTGATAGCCGCCAATCACGGTAATATGAATGTATTAGGGATTTCATGTATAACGAATATGGCTGCAGGAATTCAA
GATAAACGTTTAACTCATAAAGAAGTTGAAGAAGTTACAACTAGAGTTCGGGGCGAATTTCAGAGTTTAGTAAGAAAAGT
GTTAGCATCAATGTAA

Upstream 100 bases:

>100_bases
CTTACTTAACGTTGGTGCAACTGCCCATGGTAGCAGCTTTAAAGATCAGCTAAATTTTAGATAAAGATTTCTATATTTCT
ATATAAAGGAGGTTTTTAAT

Downstream 100 bases:

>100_bases
ATTTACTATAACAACTGCTATTAAAGGAAATATTCAAACTACTTGAGATGGCGTTATTACAGGGAGGCCTTAGAATGCGA
GCTTATGAAGTCATTGTAAA

Product: inosine guanosine and xanthosine phosphorylase family

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MDFHKITEAKEYILNQCDEVPSIGLILGSGLGSLADEIENVNYIPYQDIPHFPESTVKGHKGRLAIGKLESKTVMAMQGR
FHYYEGYDLKEVTFPVRVMQNMGIDKLIVTNAAGGIDLNFSVGGLMLITDHINFFGANPLRGKNDDRLGTRFPDMTYAYD
PELREVAKQAANELDIGLYEGVYLGVPGPSYETPAEIKMARTIGASAVGMSTVPEVIAANHGNMNVLGISCITNMAAGIQ
DKRLTHKEVEEVTTRVRGEFQSLVRKVLASM

Sequences:

>Translated_271_residues
MDFHKITEAKEYILNQCDEVPSIGLILGSGLGSLADEIENVNYIPYQDIPHFPESTVKGHKGRLAIGKLESKTVMAMQGR
FHYYEGYDLKEVTFPVRVMQNMGIDKLIVTNAAGGIDLNFSVGGLMLITDHINFFGANPLRGKNDDRLGTRFPDMTYAYD
PELREVAKQAANELDIGLYEGVYLGVPGPSYETPAEIKMARTIGASAVGMSTVPEVIAANHGNMNVLGISCITNMAAGIQ
DKRLTHKEVEEVTTRVRGEFQSLVRKVLASM
>Mature_271_residues
MDFHKITEAKEYILNQCDEVPSIGLILGSGLGSLADEIENVNYIPYQDIPHFPESTVKGHKGRLAIGKLESKTVMAMQGR
FHYYEGYDLKEVTFPVRVMQNMGIDKLIVTNAAGGIDLNFSVGGLMLITDHINFFGANPLRGKNDDRLGTRFPDMTYAYD
PELREVAKQAANELDIGLYEGVYLGVPGPSYETPAEIKMARTIGASAVGMSTVPEVIAANHGNMNVLGISCITNMAAGIQ
DKRLTHKEVEEVTTRVRGEFQSLVRKVLASM

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=272, Percent_Identity=46.6911764705882, Blast_Score=236, Evalue=1e-62,
Organism=Escherichia coli, GI1788746, Length=247, Percent_Identity=43.3198380566802, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17541190, Length=255, Percent_Identity=43.5294117647059, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6323238, Length=276, Percent_Identity=42.7536231884058, Blast_Score=196, Evalue=4e-51,
Organism=Drosophila melanogaster, GI45552887, Length=275, Percent_Identity=45.8181818181818, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI45552885, Length=275, Percent_Identity=45.8181818181818, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24656093, Length=275, Percent_Identity=45.8181818181818, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24656090, Length=275, Percent_Identity=45.8181818181818, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24762376, Length=258, Percent_Identity=37.2093023255814, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29759; Mature: 29759

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFHKITEAKEYILNQCDEVPSIGLILGSGLGSLADEIENVNYIPYQDIPHFPESTVKGH
CCCHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHCCCC
KGRLAIGKLESKTVMAMQGRFHYYEGYDLKEVTFPVRVMQNMGIDKLIVTNAAGGIDLNF
CCCEEEECCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEE
SVGGLMLITDHINFFGANPLRGKNDDRLGTRFPDMTYAYDPELREVAKQAANELDIGLYE
CCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEC
GVYLGVPGPSYETPAEIKMARTIGASAVGMSTVPEVIAANHGNMNVLGISCITNMAAGIQ
CEEEECCCCCCCCCHHHHHHHHHCCHHCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCC
DKRLTHKEVEEVTTRVRGEFQSLVRKVLASM
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFHKITEAKEYILNQCDEVPSIGLILGSGLGSLADEIENVNYIPYQDIPHFPESTVKGH
CCCHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHCCCC
KGRLAIGKLESKTVMAMQGRFHYYEGYDLKEVTFPVRVMQNMGIDKLIVTNAAGGIDLNF
CCCEEEECCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEE
SVGGLMLITDHINFFGANPLRGKNDDRLGTRFPDMTYAYDPELREVAKQAANELDIGLYE
CCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEC
GVYLGVPGPSYETPAEIKMARTIGASAVGMSTVPEVIAANHGNMNVLGISCITNMAAGIQ
CEEEECCCCCCCCCHHHHHHHHHCCHHCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCC
DKRLTHKEVEEVTTRVRGEFQSLVRKVLASM
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]