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The map label for this gene is ribE [H]

Identifier: 188584765

GI number: 188584765

Start: 146705

End: 147424

Strand: Direct

Name: ribE [H]

Synonym: Nther_0123

Alternate gene names: 188584765

Gene position: 146705-147424 (Clockwise)

Preceding gene: 188584764

Following gene: 188584766

Centisome position: 4.63

GC content: 34.72

Gene sequence:

>720_bases
ATGAAAGTAGGTGGTAACTTGTTTACCGGCATTGTAGAAGAAGTTGGAAAAGTATTGTCTGTTAATCAAGGCCCAAATTC
TATTAGTTTGAATATTCAAGCTGAAAAAATAATGGATGATATTAAATTAGGTGATAGTATTTCCACAAATGGTGTATGTT
TAACTGTTTCTAAAGTAGGAACAAATAACTTTGTGGCTGATGTTATGCCCGAAACTCTGCGTAGAAGCAATCTAAGATAC
CTTACAACTAATAGTTGGGTTAATTTAGAGCGAGCACTTAGACTCAGTGATCGCTTAGGTGGTCATTTTGTATCAGGTCA
TGTTGACGGTGTAGCGAAAGTGATCTCTAGAAAACACGAAGGAAATGCTGAAATTTTTAACTTTCAAGTTGTTGAAGAAA
ATGAACGAAATATCCAAAAGTATCTCGTAGAAAAAGGGTCTATAGCGGTGGATGGAATTAGCTTAACCATAGTGGCCTGT
GAAGGTGATGGCTTTTCTGTAAGTATAGTTCCGCACTCTAAAGAAAATACAATCTTAGAGACAAGACAAGTGGGTAATTA
TGTAAATATTGAAGTAGATATTATTGGAAAGTATGTAGAAAAATTACTGTTTCAGGAAAAACAAGATAATGGCTCTGCCA
GCACAACTGGTACTTCTGATAAAAAAGCCAAAATTGATTCCAAGATTGATATTAATCGCTTAAGAGAGCTTGGTTATTAA

Upstream 100 bases:

>100_bases
TTCAAGCGCTAAAAGACAGCGTGATGTTAAATAATATGGAATATGAATATTTTGGTGATTCTATCAGGATTGAAGCGTAT
CCATTTCTAGAAAACCAAAG

Downstream 100 bases:

>100_bases
CAGTTCCACTATCAAACTCAAGAGGCAATTGAGTTCTAGAATAATTAATTAACCTTGATACAAGAGGGGTGAATATAAAT
GACTTTAAGTACCATTAAAG

Product: riboflavin synthase, alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKVGGNLFTGIVEEVGKVLSVNQGPNSISLNIQAEKIMDDIKLGDSISTNGVCLTVSKVGTNNFVADVMPETLRRSNLRY
LTTNSWVNLERALRLSDRLGGHFVSGHVDGVAKVISRKHEGNAEIFNFQVVEENERNIQKYLVEKGSIAVDGISLTIVAC
EGDGFSVSIVPHSKENTILETRQVGNYVNIEVDIIGKYVEKLLFQEKQDNGSASTTGTSDKKAKIDSKIDINRLRELGY

Sequences:

>Translated_239_residues
MKVGGNLFTGIVEEVGKVLSVNQGPNSISLNIQAEKIMDDIKLGDSISTNGVCLTVSKVGTNNFVADVMPETLRRSNLRY
LTTNSWVNLERALRLSDRLGGHFVSGHVDGVAKVISRKHEGNAEIFNFQVVEENERNIQKYLVEKGSIAVDGISLTIVAC
EGDGFSVSIVPHSKENTILETRQVGNYVNIEVDIIGKYVEKLLFQEKQDNGSASTTGTSDKKAKIDSKIDINRLRELGY
>Mature_239_residues
MKVGGNLFTGIVEEVGKVLSVNQGPNSISLNIQAEKIMDDIKLGDSISTNGVCLTVSKVGTNNFVADVMPETLRRSNLRY
LTTNSWVNLERALRLSDRLGGHFVSGHVDGVAKVISRKHEGNAEIFNFQVVEENERNIQKYLVEKGSIAVDGISLTIVAC
EGDGFSVSIVPHSKENTILETRQVGNYVNIEVDIIGKYVEKLLFQEKQDNGSASTTGTSDKKAKIDSKIDINRLRELGY

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=191, Percent_Identity=38.2198952879581, Blast_Score=131, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319733, Length=225, Percent_Identity=43.1111111111111, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 26214; Mature: 26214

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGGNLFTGIVEEVGKVLSVNQGPNSISLNIQAEKIMDDIKLGDSISTNGVCLTVSKVG
CCCCCHHHHHHHHHHHHHEECCCCCCEEEEEEEHHHHHHHHHCCCCCCCCCEEEEEEECC
TNNFVADVMPETLRRSNLRYLTTNSWVNLERALRLSDRLGGHFVSGHVDGVAKVISRKHE
CCCCHHHHHHHHHHHCCCEEEEECCEECHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCC
GNAEIFNFQVVEENERNIQKYLVEKGSIAVDGISLTIVACEGDGFSVSIVPHSKENTILE
CCCEEEEEEEEECCHHHHHHHHHHCCCEEEECEEEEEEEECCCCEEEEEEECCCCCCEEE
TRQVGNYVNIEVDIIGKYVEKLLFQEKQDNGSASTTGTSDKKAKIDSKIDINRLRELGY
HHHCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MKVGGNLFTGIVEEVGKVLSVNQGPNSISLNIQAEKIMDDIKLGDSISTNGVCLTVSKVG
CCCCCHHHHHHHHHHHHHEECCCCCCEEEEEEEHHHHHHHHHCCCCCCCCCEEEEEEECC
TNNFVADVMPETLRRSNLRYLTTNSWVNLERALRLSDRLGGHFVSGHVDGVAKVISRKHE
CCCCHHHHHHHHHHHCCCEEEEECCEECHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCC
GNAEIFNFQVVEENERNIQKYLVEKGSIAVDGISLTIVACEGDGFSVSIVPHSKENTILE
CCCEEEEEEEEECCHHHHHHHHHHCCCEEEECEEEEEEEECCCCEEEEEEECCCCCCEEE
TRQVGNYVNIEVDIIGKYVEKLLFQEKQDNGSASTTGTSDKKAKIDSKIDINRLRELGY
HHHCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]