Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 188584721

GI number: 188584721

Start: 95138

End: 96667

Strand: Direct

Name: tilS [H]

Synonym: Nther_0079

Alternate gene names: 188584721

Gene position: 95138-96667 (Clockwise)

Preceding gene: 188584720

Following gene: 188584722

Centisome position: 3.01

GC content: 33.27

Gene sequence:

>1530_bases
ATGAAAGAAAAAGTTTATCAAAACATTTTTGAAAAAGGGTTAATAAAATCTGGAGACAAGGTATTAGTAGGAGTTTCTGG
AGGGCCTGATTCAGTAGCCCTCCTTCATGTGTTATATGAACTAAGGCAAGAACTTCAGTTCAAATTAGTAGTTTCACATT
TAAATCATGGTTTTAGGGATAAAAGTGCTGAAGAAGATGCTGATTTTGTAAAAAAACTGGCCGAAGATTTGGAATTACCG
ATAATTTACGACAAATGGCAGGTAAAAGATTATATTAATTACTATAAACTTTCACCACAACAAGGGGCCCGAATTCAAAG
GTATAAATTTTTTGAACAAGCTGCAAAAGATTTCGGTGCAACTAAATTAGCTCTGGGCCATCATATAGATGATCAAGTGG
AAACCTTTTTTTTACGCTTGATAAGAGGTGCTGGAACTCAAGGCCTTTCAGGTATGCAATTTATAAGAAAGTTAAATAAT
GATCTGCTGCTAATCAGGCCGTTGTTAGAGGTGTCAAAAAGTGAAATTAAAGAGTATTTACAAACTAATAAACTAAAATA
TAAAATAGATCCAACAAATCAAGAGACCAATTATCTTAGGAATAAGGTGAGACATGAAATTTTACCTGTCTTTCAGGATA
TAAACCCCAATATTAATGAAAGACTTTTAGAAACAATGGAGTTGTTAAAAGAAGAAAATGACTACTTAAACAAGGCAGCT
AACGAGCTATATCAAAAGTCACAAGTTGATACTGGACGGAGTCAAAATAATACAAAACCCCTGGCTTACCAAGGACAAGA
AAGAGAACTATTATTTTGCCTTAAAACATTACAAAAATCGCCTAAAGTGTTGTTACGCAGAATCTTATTAACAGCAGTTT
ATTCTTTTATTTCTAAAACTGACCAAGAGGACAGGCATCTAAATTCAGAAAATGTTTCAAAAAAACATATAGATGCCGTG
GCGAAGATGATTGAGTTAGGCTTAACAGGCAAAGAAATAACATTACCAGGAAAAATCTACACTTTTATAGATACTCGAAT
GATTTCAGGTCAGTCAAAACAAGTGATGGTAATTTCAGATCAAAATTTAACATCATCATATGCTATTCAAGGGACTAAAC
AGGCAGAATTAGAAGTCCCCGGGTGGACTTACTGGCCTCTATCAAATATGTGGCTTTATAGTTATATAAAAGATCACAAA
TCAGAGCAATCGAGTCATTATCACAAAACAATTAACAGAAATTCTTTTGAAGCTACATTAGATTATGATAAGATTGATTT
ACCAGTAATAATAAGAAATAGAAAGGTGGGGGATCGGTTTGTTCCATTGGGAATGAATTCCCCCAAAAAATTAAAAGACT
TTTTTATTGATGAAAAAGTACCCAGAAATTATCGTGACAAAATTCCTTTGTTAGTTAGCGGCCGAGGTGAAATATTGTGG
GTGATAGGATATCGCATAAATGAACAGTATAAAGTTACTAAGGATACTAGAAGATATTTTTGCATTGAAGCTCAACTATC
GGAGTATTAA

Upstream 100 bases:

>100_bases
GGGATTAGATATATGGGAAAAGATAAATTAATGAAAGCAGGAATTAAATAAAAAATGACGAATTAACGTATATATGAAAG
GATAGTATGAGGGGATTACT

Downstream 100 bases:

>100_bases
TTTAAAAATTCAGGTAATGAATAAAAGGAGGGTATACTGTGGAAAGGGCTTATGACAAAGATGCTTTTAAACAATTAATT
AGTGAAGAGGAAATAAAAGA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKEKVYQNIFEKGLIKSGDKVLVGVSGGPDSVALLHVLYELRQELQFKLVVSHLNHGFRDKSAEEDADFVKKLAEDLELP
IIYDKWQVKDYINYYKLSPQQGARIQRYKFFEQAAKDFGATKLALGHHIDDQVETFFLRLIRGAGTQGLSGMQFIRKLNN
DLLLIRPLLEVSKSEIKEYLQTNKLKYKIDPTNQETNYLRNKVRHEILPVFQDINPNINERLLETMELLKEENDYLNKAA
NELYQKSQVDTGRSQNNTKPLAYQGQERELLFCLKTLQKSPKVLLRRILLTAVYSFISKTDQEDRHLNSENVSKKHIDAV
AKMIELGLTGKEITLPGKIYTFIDTRMISGQSKQVMVISDQNLTSSYAIQGTKQAELEVPGWTYWPLSNMWLYSYIKDHK
SEQSSHYHKTINRNSFEATLDYDKIDLPVIIRNRKVGDRFVPLGMNSPKKLKDFFIDEKVPRNYRDKIPLLVSGRGEILW
VIGYRINEQYKVTKDTRRYFCIEAQLSEY

Sequences:

>Translated_509_residues
MKEKVYQNIFEKGLIKSGDKVLVGVSGGPDSVALLHVLYELRQELQFKLVVSHLNHGFRDKSAEEDADFVKKLAEDLELP
IIYDKWQVKDYINYYKLSPQQGARIQRYKFFEQAAKDFGATKLALGHHIDDQVETFFLRLIRGAGTQGLSGMQFIRKLNN
DLLLIRPLLEVSKSEIKEYLQTNKLKYKIDPTNQETNYLRNKVRHEILPVFQDINPNINERLLETMELLKEENDYLNKAA
NELYQKSQVDTGRSQNNTKPLAYQGQERELLFCLKTLQKSPKVLLRRILLTAVYSFISKTDQEDRHLNSENVSKKHIDAV
AKMIELGLTGKEITLPGKIYTFIDTRMISGQSKQVMVISDQNLTSSYAIQGTKQAELEVPGWTYWPLSNMWLYSYIKDHK
SEQSSHYHKTINRNSFEATLDYDKIDLPVIIRNRKVGDRFVPLGMNSPKKLKDFFIDEKVPRNYRDKIPLLVSGRGEILW
VIGYRINEQYKVTKDTRRYFCIEAQLSEY
>Mature_509_residues
MKEKVYQNIFEKGLIKSGDKVLVGVSGGPDSVALLHVLYELRQELQFKLVVSHLNHGFRDKSAEEDADFVKKLAEDLELP
IIYDKWQVKDYINYYKLSPQQGARIQRYKFFEQAAKDFGATKLALGHHIDDQVETFFLRLIRGAGTQGLSGMQFIRKLNN
DLLLIRPLLEVSKSEIKEYLQTNKLKYKIDPTNQETNYLRNKVRHEILPVFQDINPNINERLLETMELLKEENDYLNKAA
NELYQKSQVDTGRSQNNTKPLAYQGQERELLFCLKTLQKSPKVLLRRILLTAVYSFISKTDQEDRHLNSENVSKKHIDAV
AKMIELGLTGKEITLPGKIYTFIDTRMISGQSKQVMVISDQNLTSSYAIQGTKQAELEVPGWTYWPLSNMWLYSYIKDHK
SEQSSHYHKTINRNSFEATLDYDKIDLPVIIRNRKVGDRFVPLGMNSPKKLKDFFIDEKVPRNYRDKIPLLVSGRGEILW
VIGYRINEQYKVTKDTRRYFCIEAQLSEY

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=218, Percent_Identity=31.1926605504587, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787606, Length=201, Percent_Identity=28.8557213930348, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 59267; Mature: 59267

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKVYQNIFEKGLIKSGDKVLVGVSGGPDSVALLHVLYELRQELQFKLVVSHLNHGFRD
CCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KSAEEDADFVKKLAEDLELPIIYDKWQVKDYINYYKLSPQQGARIQRYKFFEQAAKDFGA
CCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
TKLALGHHIDDQVETFFLRLIRGAGTQGLSGMQFIRKLNNDLLLIRPLLEVSKSEIKEYL
EEEHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
QTNKLKYKIDPTNQETNYLRNKVRHEILPVFQDINPNINERLLETMELLKEENDYLNKAA
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NELYQKSQVDTGRSQNNTKPLAYQGQERELLFCLKTLQKSPKVLLRRILLTAVYSFISKT
HHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
DQEDRHLNSENVSKKHIDAVAKMIELGLTGKEITLPGKIYTFIDTRMISGQSKQVMVISD
CCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEHHHEECCCCCEEEEEEC
QNLTSSYAIQGTKQAELEVPGWTYWPLSNMWLYSYIKDHKSEQSSHYHKTINRNSFEATL
CCCCCCCEECCCCCCEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
DYDKIDLPVIIRNRKVGDRFVPLGMNSPKKLKDFFIDEKVPRNYRDKIPLLVSGRGEILW
CCCCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHCCCCCCCCHHCCCCEEEECCCCEEE
VIGYRINEQYKVTKDTRRYFCIEAQLSEY
EEEEEECCCEEECCCCCEEEEEEECCCCC
>Mature Secondary Structure
MKEKVYQNIFEKGLIKSGDKVLVGVSGGPDSVALLHVLYELRQELQFKLVVSHLNHGFRD
CCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KSAEEDADFVKKLAEDLELPIIYDKWQVKDYINYYKLSPQQGARIQRYKFFEQAAKDFGA
CCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
TKLALGHHIDDQVETFFLRLIRGAGTQGLSGMQFIRKLNNDLLLIRPLLEVSKSEIKEYL
EEEHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
QTNKLKYKIDPTNQETNYLRNKVRHEILPVFQDINPNINERLLETMELLKEENDYLNKAA
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NELYQKSQVDTGRSQNNTKPLAYQGQERELLFCLKTLQKSPKVLLRRILLTAVYSFISKT
HHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
DQEDRHLNSENVSKKHIDAVAKMIELGLTGKEITLPGKIYTFIDTRMISGQSKQVMVISD
CCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEHHHEECCCCCEEEEEEC
QNLTSSYAIQGTKQAELEVPGWTYWPLSNMWLYSYIKDHKSEQSSHYHKTINRNSFEATL
CCCCCCCEECCCCCCEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
DYDKIDLPVIIRNRKVGDRFVPLGMNSPKKLKDFFIDEKVPRNYRDKIPLLVSGRGEILW
CCCCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHCCCCCCCCHHCCCCEEEECCCCEEE
VIGYRINEQYKVTKDTRRYFCIEAQLSEY
EEEEEECCCEEECCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA