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The map label for this gene is dnaN [H]

Identifier: 188584644

GI number: 188584644

Start: 1780

End: 2892

Strand: Direct

Name: dnaN [H]

Synonym: Nther_0002

Alternate gene names: 188584644

Gene position: 1780-2892 (Clockwise)

Preceding gene: 188584643

Following gene: 188584645

Centisome position: 0.06

GC content: 31.36

Gene sequence:

>1113_bases
ATGAAAATCATTTTTCAAAAAGATGAACTTACTGAAGCTTTGCAAGCAACGCAAAGAGGTATTTCAAATAAAAATACAAT
TCCCCATTTAAATGGCTTTTTTATTCAGACCCATAGCGATAATACTCTCTCTATCATTACGTATGACTTAGAAATGGGTA
TAAACTACTATTTATCAACAGAAATTTTAGAACAAGGTTCCGTTGTTGTACCTACTAAGTTTTTTGATATAATAAAAAAG
CTGCCGGAAGATCAAATTACTTTTAGTGTTGATCAGGAAAATTATTTAATCACTATTGAAAGCGGACAAGTTTACTATGA
AATCAAAGGTCTTGATCCTGAAGAATACCCCAAATTACCAGAAATTGAAGAAGGGAAATCTTTTTCTATTCCGGAAATCT
TATTGAAAAAAATGATTCATCAAACCAGTTTTGCAGTTAGCAAAGAAGAGACTAAACCTGCATTTACAGGAGTTCTTTGT
AAATTTGATTCAGATAATAAACTTGATATGGTTGCAACTGATAGTTATAGATTAGCTTGGAGAAAAGGTAAAATTTCTAA
TGATAGTCAAATATCAGGAGAGTATATAATACCGCAGAAAGCTATTGGTCAAGTACAGAGAATTATAAGCGAAGAGGATA
AATTAATTAACTTGTATTTAGCAAACGGATATTTCTTGTGTGAAACTGAAAAAGTAAGGCTATTTAGTAAGTTAATTGAC
GAGGAATTTCCCAATCTGGATCAAGTAATACCAAAAAATTATCAAACCAAAGTAATGATTGACAGAAAATTCCTGCAAGA
TTCTATGGAAAGAGCTTCTCTTTTAGCCAGTGAAGGTGCAAATAACATTGTTAAATTAAACATTTCAGAAGAAAATATGG
AAATTACATCTAATTCCCCTCATACGGGTAAATTAAAAGAAGATTTACAATTATATAAAGAAGGAGATAACATAAGTATC
GCCCTTAATGCCCGATTTATCATTGAAGTTTTAAAGGTCATTGAAGAAGATCAAGTAGAATTAGAATTTACTGGCTCTTA
CAGCCCTATGATCATCCGTCCTAGGGAAAATGATCAGTACTTTCATCTCATACTGCCAGTAAGAGCTTTTTAA

Upstream 100 bases:

>100_bases
GGGTCTATTTTCCACTTATCCACAGGCCTTACTGTTAAGATGACTGTATTTACTAAGTATATTAATTATATAAAGATAAT
ATGACAAAGGAGTGAAACAA

Downstream 100 bases:

>100_bases
ACTGTTTAGGAGTGATATTATGAGTAAGCGTAAAGTTGAGATTAATAGTGAATATATCACCTTAGGGCAATTGTTGAAGT
ATGTAAACATTGTTGCTACC

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKIIFQKDELTEALQATQRGISNKNTIPHLNGFFIQTHSDNTLSIITYDLEMGINYYLSTEILEQGSVVVPTKFFDIIKK
LPEDQITFSVDQENYLITIESGQVYYEIKGLDPEEYPKLPEIEEGKSFSIPEILLKKMIHQTSFAVSKEETKPAFTGVLC
KFDSDNKLDMVATDSYRLAWRKGKISNDSQISGEYIIPQKAIGQVQRIISEEDKLINLYLANGYFLCETEKVRLFSKLID
EEFPNLDQVIPKNYQTKVMIDRKFLQDSMERASLLASEGANNIVKLNISEENMEITSNSPHTGKLKEDLQLYKEGDNISI
ALNARFIIEVLKVIEEDQVELEFTGSYSPMIIRPRENDQYFHLILPVRAF

Sequences:

>Translated_370_residues
MKIIFQKDELTEALQATQRGISNKNTIPHLNGFFIQTHSDNTLSIITYDLEMGINYYLSTEILEQGSVVVPTKFFDIIKK
LPEDQITFSVDQENYLITIESGQVYYEIKGLDPEEYPKLPEIEEGKSFSIPEILLKKMIHQTSFAVSKEETKPAFTGVLC
KFDSDNKLDMVATDSYRLAWRKGKISNDSQISGEYIIPQKAIGQVQRIISEEDKLINLYLANGYFLCETEKVRLFSKLID
EEFPNLDQVIPKNYQTKVMIDRKFLQDSMERASLLASEGANNIVKLNISEENMEITSNSPHTGKLKEDLQLYKEGDNISI
ALNARFIIEVLKVIEEDQVELEFTGSYSPMIIRPRENDQYFHLILPVRAF
>Mature_370_residues
MKIIFQKDELTEALQATQRGISNKNTIPHLNGFFIQTHSDNTLSIITYDLEMGINYYLSTEILEQGSVVVPTKFFDIIKK
LPEDQITFSVDQENYLITIESGQVYYEIKGLDPEEYPKLPEIEEGKSFSIPEILLKKMIHQTSFAVSKEETKPAFTGVLC
KFDSDNKLDMVATDSYRLAWRKGKISNDSQISGEYIIPQKAIGQVQRIISEEDKLINLYLANGYFLCETEKVRLFSKLID
EEFPNLDQVIPKNYQTKVMIDRKFLQDSMERASLLASEGANNIVKLNISEENMEITSNSPHTGKLKEDLQLYKEGDNISI
ALNARFIIEVLKVIEEDQVELEFTGSYSPMIIRPRENDQYFHLILPVRAF

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=370, Percent_Identity=26.2162162162162, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42511; Mature: 42511

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIFQKDELTEALQATQRGISNKNTIPHLNGFFIQTHSDNTLSIITYDLEMGINYYLST
CEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEEEEECCCEEEEEH
EILEQGSVVVPTKFFDIIKKLPEDQITFSVDQENYLITIESGQVYYEIKGLDPEEYPKLP
HHHHCCCEEECHHHHHHHHHCCCCCEEEEECCCCEEEEEECCEEEEEEECCCHHHCCCCC
EIEEGKSFSIPEILLKKMIHQTSFAVSKEETKPAFTGVLCKFDSDNKLDMVATDSYRLAW
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEEECCEEEEE
RKGKISNDSQISGEYIIPQKAIGQVQRIISEEDKLINLYLANGYFLCETEKVRLFSKLID
ECCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCEEEEEEEECCEEEEECHHHHHHHHHHH
EEFPNLDQVIPKNYQTKVMIDRKFLQDSMERASLLASEGANNIVKLNISEENMEITSNSP
HHCCCHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCC
HTGKLKEDLQLYKEGDNISIALNARFIIEVLKVIEEDQVELEFTGSYSPMIIRPRENDQY
CCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCE
FHLILPVRAF
EEEEEEEECC
>Mature Secondary Structure
MKIIFQKDELTEALQATQRGISNKNTIPHLNGFFIQTHSDNTLSIITYDLEMGINYYLST
CEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEEEEECCCEEEEEH
EILEQGSVVVPTKFFDIIKKLPEDQITFSVDQENYLITIESGQVYYEIKGLDPEEYPKLP
HHHHCCCEEECHHHHHHHHHCCCCCEEEEECCCCEEEEEECCEEEEEEECCCHHHCCCCC
EIEEGKSFSIPEILLKKMIHQTSFAVSKEETKPAFTGVLCKFDSDNKLDMVATDSYRLAW
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEEECCEEEEE
RKGKISNDSQISGEYIIPQKAIGQVQRIISEEDKLINLYLANGYFLCETEKVRLFSKLID
ECCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCEEEEEEEECCEEEEECHHHHHHHHHHH
EEFPNLDQVIPKNYQTKVMIDRKFLQDSMERASLLASEGANNIVKLNISEENMEITSNSP
HHCCCHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCC
HTGKLKEDLQLYKEGDNISIALNARFIIEVLKVIEEDQVELEFTGSYSPMIIRPRENDQY
CCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCE
FHLILPVRAF
EEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]