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The map label for this gene is folK [H]

Identifier: 188527220

GI number: 188527220

Start: 406953

End: 407441

Strand: Direct

Name: folK [H]

Synonym: HPSH_02135

Alternate gene names: 188527220

Gene position: 406953-407441 (Clockwise)

Preceding gene: 188527219

Following gene: 188527221

Centisome position: 25.3

GC content: 35.38

Gene sequence:

>489_bases
ATGCGAGAGATCCTTACTAGCCGCTTTTTCCCTAGCCTTTTTAAAAAAAGGCTTGATTTTTCTAACAGGGTGGTTTTAGG
GCTAGGATCTAATCTTAAAAATCCTTTGAAAATATTAAAAAATTGTTTTTTATATTTTAAAAATCATAGTAAAATCGGGA
AAATTCTTTCTTCGCCTGTTTATATCAACCCGCCTTTTGGTTACACGAAGCAGCCTAACTTTTATAACGCTACGATTATC
CTTAAAACATCTTTAAGTTTGCACCATTTTTTCGCTCTGGTTTTTTATATAGAAAGGCGTTTTGGGCGCCAAAGGAAGCG
CGATTTTAAAGACGCTCCAAGAACTTTAGACATTGATATTATCGCTTTCAATCAAGTCATTTTAAGACAGAACGATTTGA
CTTTACCTCATCCTAAATGGAGTGAAAGGGATTCGGTGTTAGTGCCGTTGGCTTTGCAACAAATTCTTTTTAAAAAAGGG
GAGTGGTGA

Upstream 100 bases:

>100_bases
TGGTGTTTTCTGTAGAGCCTGGGATTTATATCCCTGGGTTTTTTGGGGTGCGCATTGAAGATTTAGTGGTGATCAAAAAT
TCTAGGGCTGAGCTTTTGTG

Downstream 100 bases:

>100_bases
AATTCTATACCTATAGTGGGGAGACGGCCGCTGAAGCTTTAAAGATCGCTCAAAGCCACCATGGGGTGGATACGCTGGTG
TTTAAAACCCAAGAAATCCG

Product: 7, 8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (folK)

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MREILTSRFFPSLFKKRLDFSNRVVLGLGSNLKNPLKILKNCFLYFKNHSKIGKILSSPVYINPPFGYTKQPNFYNATII
LKTSLSLHHFFALVFYIERRFGRQRKRDFKDAPRTLDIDIIAFNQVILRQNDLTLPHPKWSERDSVLVPLALQQILFKKG
EW

Sequences:

>Translated_162_residues
MREILTSRFFPSLFKKRLDFSNRVVLGLGSNLKNPLKILKNCFLYFKNHSKIGKILSSPVYINPPFGYTKQPNFYNATII
LKTSLSLHHFFALVFYIERRFGRQRKRDFKDAPRTLDIDIIAFNQVILRQNDLTLPHPKWSERDSVLVPLALQQILFKKG
EW
>Mature_162_residues
MREILTSRFFPSLFKKRLDFSNRVVLGLGSNLKNPLKILKNCFLYFKNHSKIGKILSSPVYINPPFGYTKQPNFYNATII
LKTSLSLHHFFALVFYIERRFGRQRKRDFKDAPRTLDIDIIAFNQVILRQNDLTLPHPKWSERDSVLVPLALQQILFKKG
EW

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=138, Percent_Identity=31.8840579710145, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 19114; Mature: 19114

Theoretical pI: Translated: 11.00; Mature: 11.00

Prosite motif: PS00092 N6_MTASE ; PS00794 HPPK ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREILTSRFFPSLFKKRLDFSNRVVLGLGSNLKNPLKILKNCFLYFKNHSKIGKILSSPV
CHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCE
YINPPFGYTKQPNFYNATIILKTSLSLHHFFALVFYIERRFGRQRKRDFKDAPRTLDIDI
EECCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
IAFNQVILRQNDLTLPHPKWSERDSVLVPLALQQILFKKGEW
EEHHHHEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCC
>Mature Secondary Structure
MREILTSRFFPSLFKKRLDFSNRVVLGLGSNLKNPLKILKNCFLYFKNHSKIGKILSSPV
CHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCE
YINPPFGYTKQPNFYNATIILKTSLSLHHFFALVFYIERRFGRQRKRDFKDAPRTLDIDI
EECCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
IAFNQVILRQNDLTLPHPKWSERDSVLVPLALQQILFKKGEW
EEHHHHEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]