Click here to switch to the map view.

The map label for this gene is pflA [H]

Identifier: 187935671

GI number: 187935671

Start: 2680856

End: 2681566

Strand: Reverse

Name: pflA [H]

Synonym: CLL_A2572

Alternate gene names: 187935671

Gene position: 2681566-2680856 (Counterclockwise)

Preceding gene: 187933107

Following gene: 187934898

Centisome position: 70.56

GC content: 28.27

Gene sequence:

>711_bases
ATGGTTAAAGGAAGAATTCATTCAATAGAGACAATGGGACTAGTAGATGGACCTGGAATTAGAGTGGTTGTATTTTTTCA
AGGATGTTCATTAAGATGTAAGTACTGTCATAATCCAGATACGTGGACATATGATGGAGGAGATGAATATACTCCAGAAG
AACTAGTGAAAAAAATAGAGAGATATAAAACGTATTTTAAAAGTTCTAATGGCGGAGTTACATTTTCAGGAGGAGAACCT
CTAAGACAACCAGAATTTCTTCTAGAAGTATTAAAATTATGTAAAGAAAAAGGAATACACACTTGTTTAGATACTTCGGG
ATTTGGTATAGGAGATTATAATGAAATATTAAAATGTGTAGATTTAGTTTTATTTGATGTAAAACATTATAATAAAGAAG
GCTATAAAAATGTAACTTACATGGATATAAATAAATCATTAAAGTTTTTAAATTCAGTTCAACAATTTAAGATACCTATT
TGGATTAGACATGTTGTTGTTCCAGGATTAACAGATGGTGAAGAACATATAAGAAATTTAAAAAAATACATATCCAATAT
AGATGGAGTTGAGAAAGTAGAATTACTGCCATATCATTTGTTAGGGAAAAACAAGTATGATGTTTTAAAATTTAAATATC
CATTAGAAGGAGTTCCTGCTATGGATAAAGAAATAACTAAAAAGTATCAAAGAATTATTGAAGATAAATAG

Upstream 100 bases:

>100_bases
ATAATCAATAAAAGTATATATACTATAAATTCATAAACATACTTAATTATACCCCTGTATTAAATGACAGGGGTGTAGTT
TTCTCTAGGAGGATAAAAAT

Downstream 100 bases:

>100_bases
AAATAAGGAGCTTTTAAAATAAAAAGCTCCTTATTTTTTTCTTTAGAAAATTATATAATCTTAAAATTTATAGATAACTC
ATAGAATACTATATTTTAGA

Product: pyruvate formate-lyase 1-activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MVKGRIHSIETMGLVDGPGIRVVVFFQGCSLRCKYCHNPDTWTYDGGDEYTPEELVKKIERYKTYFKSSNGGVTFSGGEP
LRQPEFLLEVLKLCKEKGIHTCLDTSGFGIGDYNEILKCVDLVLFDVKHYNKEGYKNVTYMDINKSLKFLNSVQQFKIPI
WIRHVVVPGLTDGEEHIRNLKKYISNIDGVEKVELLPYHLLGKNKYDVLKFKYPLEGVPAMDKEITKKYQRIIEDK

Sequences:

>Translated_236_residues
MVKGRIHSIETMGLVDGPGIRVVVFFQGCSLRCKYCHNPDTWTYDGGDEYTPEELVKKIERYKTYFKSSNGGVTFSGGEP
LRQPEFLLEVLKLCKEKGIHTCLDTSGFGIGDYNEILKCVDLVLFDVKHYNKEGYKNVTYMDINKSLKFLNSVQQFKIPI
WIRHVVVPGLTDGEEHIRNLKKYISNIDGVEKVELLPYHLLGKNKYDVLKFKYPLEGVPAMDKEITKKYQRIIEDK
>Mature_236_residues
MVKGRIHSIETMGLVDGPGIRVVVFFQGCSLRCKYCHNPDTWTYDGGDEYTPEELVKKIERYKTYFKSSNGGVTFSGGEP
LRQPEFLLEVLKLCKEKGIHTCLDTSGFGIGDYNEILKCVDLVLFDVKHYNKEGYKNVTYMDINKSLKFLNSVQQFKIPI
WIRHVVVPGLTDGEEHIRNLKKYISNIDGVEKVELLPYHLLGKNKYDVLKFKYPLEGVPAMDKEITKKYQRIIEDK

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=237, Percent_Identity=44.3037974683544, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1790389, Length=248, Percent_Identity=27.0161290322581, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI226510931, Length=168, Percent_Identity=31.547619047619, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27210; Mature: 27210

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKGRIHSIETMGLVDGPGIRVVVFFQGCSLRCKYCHNPDTWTYDGGDEYTPEELVKKIE
CCCCCCCCEEEECCCCCCCCEEEEEEECCCEEEEECCCCCCEEECCCCCCCHHHHHHHHH
RYKTYFKSSNGGVTFSGGEPLRQPEFLLEVLKLCKEKGIHTCLDTSGFGIGDYNEILKCV
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHH
DLVLFDVKHYNKEGYKNVTYMDINKSLKFLNSVQQFKIPIWIRHVVVPGLTDGEEHIRNL
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHH
KKYISNIDGVEKVELLPYHLLGKNKYDVLKFKYPLEGVPAMDKEITKKYQRIIEDK
HHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKGRIHSIETMGLVDGPGIRVVVFFQGCSLRCKYCHNPDTWTYDGGDEYTPEELVKKIE
CCCCCCCCEEEECCCCCCCCEEEEEEECCCEEEEECCCCCCEEECCCCCCCHHHHHHHHH
RYKTYFKSSNGGVTFSGGEPLRQPEFLLEVLKLCKEKGIHTCLDTSGFGIGDYNEILKCV
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHH
DLVLFDVKHYNKEGYKNVTYMDINKSLKFLNSVQQFKIPIWIRHVVVPGLTDGEEHIRNL
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHH
KKYISNIDGVEKVELLPYHLLGKNKYDVLKFKYPLEGVPAMDKEITKKYQRIIEDK
HHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]