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The map label for this gene is pflB [H]

Identifier: 187933107

GI number: 187933107

Start: 2681737

End: 2683965

Strand: Reverse

Name: pflB [H]

Synonym: CLL_A2573

Alternate gene names: 187933107

Gene position: 2683965-2681737 (Counterclockwise)

Preceding gene: 187934364

Following gene: 187935671

Centisome position: 70.62

GC content: 30.87

Gene sequence:

>2229_bases
ATGTTTAAACAATGGGAAGGTTTTAAAAATGGTACTTGGCAAGAAGGAATTGATGTAAGAAACTTTATACAAAAGAATTA
TAATGTGTATGAAGGAGATTCAAGCTTCTTAGAAGGTATAAGTGAAAAGACATCAAGAGTATGGGACAAAGCATACGAAT
TAATTGTTGAAGAAGTTAAAAAAGGAATAATAGATATTGCAACCGACAGAGTTTCAGGAATAAATAACTATGAAGCAGGA
TATATAGATAAAGATAATGAAGTAATTGTAGGACTTCAAACTGATGCTCCACTTAAAAGAATTGTAAATCCATTTGGTGG
ATTTAGAATGGTACAAACTTCATTAAAAGAATATGGATATGAATTAGATAAAGATATAGAAAAACATTTTTCAAGATACA
GAAAGACTCACAATGAAGGAGTTTTTGATGGCTATACAAAAGAAATAAGACTTGCAAGAACTGCAGGACTTTTAACAGGA
CTTCCAGATGCTTATGGTAGAGGAAGAATAATAGGTGATTATAGAAGAGTAGCTCTTTATGGTATAGATTATTTAATAGA
AGAAAAGAAAAATGATCTTGATAATCTTCAAGGGGACATGCTTGATGAATTAGTAAGAAAAAGAGAAGAAGTTTCTATGC
AAATTAGAGCTTTAGGAGAAATAAAAGAAATGGCAGCTAAATATGGCTGTGATATATCTAAACCAGCATCAAATGCTAAA
GAAGCTGTACAAGCTTTATACTTTGGATATTTAGCTGGAATTAAAGAAAATAATGGAGCAGCAACTTCATTTGGTAGAAC
TTCAACTTTCTTAGATATATACATTGAAAGAGATTTAGAAACAGGATTAATAACTGAAAAAGAAGCTCAAGAATTAGTAG
ATCAACTTATAATAAAATTAAGATTAGTAAGACATTTAAGAACTCCAGAATACAACGATTTATTTGGTGGAGATCCAACA
TGGGTAACTGAATCAATTGGCGGAATGGGCATAAATGGGAAATCATTAGTAACAAAGAACTCATTTAGATATTTACATAC
ATTGATAAATCTAGGTGCTTCAGCAGAACCAAACTTAACAGTTTTATGGTCAGATAAATTACCAGAAAACTTTAAAAAAT
ATTGTGCAGAAATATCAATAAAGACAGATGCAGTTCAATATGAAAGTGATGAAGTAATGAGACCAATTTATGGTGATGAT
TATGCAATAGCTTGTTGTGTATCAGCTATGAAGGTTGGTAAACAAATGCAATTCTTTGGAGCAAGATGTAATATTGCAAA
ATCACTTTTATATGCAATAAATGGTGGTTGTGATGAATTAAAAGGTATGAAAGTAATACCAGGAATTGAAGTAATGAATG
ATGAAGTTTTAGATTTCAATAAAGTAAAAGAAAATTACTTCAAAGTTTTAGAATATGTTGCTAAAATTTATGTGGATACA
ATGAATATAATACATCATATGCATGATAAATATGCTTATGAAGCAGGACAAATGGCTTTACATGATACTATGGTAGATAG
ATTAATGGCATTTGGTATTGCAGGTCTTTCAGTTGCAATAGATTCATTATCAGCTATTAAATATGCAAAAGTTAAACCAA
TAAGAAATGAAGAAGGATTAGCTATTGACTTTGAAGTTCAAGGAGATTTTCCTAAATACGGAAATGATGATGATAGAGCA
GATGATTTAGGAGTAGAATTAGTAACTAAGTTCTCAAATGAACTTAAGAGACATCCTTTATATAGAGATGCTAAACATAC
ATTATCAGCCCTTACAATTACATCTAATGTTATGTATGGTAAGAAGACTGGCACAACACCAGATGGAAGAAAGAAAGGTG
AACCGTTAGCACCAGGAGCTAATCCAATGCACGGAAGAGATGTAAATGGAGCATTGGCATCACTAAATTCAGTTGCTAAA
ATACCTTACAATGAAGTTTGTCAAGATGGTGTTTCAAACACATTCTCAATTGTACCCGATGCACTTGGAAAGAGTGAAGA
ACAAAGAATATCTAATTTAGTTTCAATTTTAGACGGATATTTTGTTCAAGGAGCACATCATTTAAATGTTAATGTTCTTA
ACAGGGAAACATTAATAGATGCTATGGAAAATCCAGATAAATATCCAACATTAACAATTAGAGTTTCTGGATATGCTGTT
AACTTTAGCAGATTATCAAAAGAACAACAATTAGAAGTTATAAGCAGAACTTTCCACGAAAGTATCTAA

Upstream 100 bases:

>100_bases
ATAGAAAAAGCTTTAAATTTAAGATAAGATATATTATAATGAGTTTGAGAAAGATTTAACAAACTTTCTAATGGTATATA
TTAAAAAGGAGGCAAAATTT

Downstream 100 bases:

>100_bases
GATTAAATAATTAAAGCTATATTTTAAATACCTGTTGATATATGCCACTTTACCTATTGCATATATCAACATAATCAATA
AAAGTATATATACTATAAAT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MFKQWEGFKNGTWQEGIDVRNFIQKNYNVYEGDSSFLEGISEKTSRVWDKAYELIVEEVKKGIIDIATDRVSGINNYEAG
YIDKDNEVIVGLQTDAPLKRIVNPFGGFRMVQTSLKEYGYELDKDIEKHFSRYRKTHNEGVFDGYTKEIRLARTAGLLTG
LPDAYGRGRIIGDYRRVALYGIDYLIEEKKNDLDNLQGDMLDELVRKREEVSMQIRALGEIKEMAAKYGCDISKPASNAK
EAVQALYFGYLAGIKENNGAATSFGRTSTFLDIYIERDLETGLITEKEAQELVDQLIIKLRLVRHLRTPEYNDLFGGDPT
WVTESIGGMGINGKSLVTKNSFRYLHTLINLGASAEPNLTVLWSDKLPENFKKYCAEISIKTDAVQYESDEVMRPIYGDD
YAIACCVSAMKVGKQMQFFGARCNIAKSLLYAINGGCDELKGMKVIPGIEVMNDEVLDFNKVKENYFKVLEYVAKIYVDT
MNIIHHMHDKYAYEAGQMALHDTMVDRLMAFGIAGLSVAIDSLSAIKYAKVKPIRNEEGLAIDFEVQGDFPKYGNDDDRA
DDLGVELVTKFSNELKRHPLYRDAKHTLSALTITSNVMYGKKTGTTPDGRKKGEPLAPGANPMHGRDVNGALASLNSVAK
IPYNEVCQDGVSNTFSIVPDALGKSEEQRISNLVSILDGYFVQGAHHLNVNVLNRETLIDAMENPDKYPTLTIRVSGYAV
NFSRLSKEQQLEVISRTFHESI

Sequences:

>Translated_742_residues
MFKQWEGFKNGTWQEGIDVRNFIQKNYNVYEGDSSFLEGISEKTSRVWDKAYELIVEEVKKGIIDIATDRVSGINNYEAG
YIDKDNEVIVGLQTDAPLKRIVNPFGGFRMVQTSLKEYGYELDKDIEKHFSRYRKTHNEGVFDGYTKEIRLARTAGLLTG
LPDAYGRGRIIGDYRRVALYGIDYLIEEKKNDLDNLQGDMLDELVRKREEVSMQIRALGEIKEMAAKYGCDISKPASNAK
EAVQALYFGYLAGIKENNGAATSFGRTSTFLDIYIERDLETGLITEKEAQELVDQLIIKLRLVRHLRTPEYNDLFGGDPT
WVTESIGGMGINGKSLVTKNSFRYLHTLINLGASAEPNLTVLWSDKLPENFKKYCAEISIKTDAVQYESDEVMRPIYGDD
YAIACCVSAMKVGKQMQFFGARCNIAKSLLYAINGGCDELKGMKVIPGIEVMNDEVLDFNKVKENYFKVLEYVAKIYVDT
MNIIHHMHDKYAYEAGQMALHDTMVDRLMAFGIAGLSVAIDSLSAIKYAKVKPIRNEEGLAIDFEVQGDFPKYGNDDDRA
DDLGVELVTKFSNELKRHPLYRDAKHTLSALTITSNVMYGKKTGTTPDGRKKGEPLAPGANPMHGRDVNGALASLNSVAK
IPYNEVCQDGVSNTFSIVPDALGKSEEQRISNLVSILDGYFVQGAHHLNVNVLNRETLIDAMENPDKYPTLTIRVSGYAV
NFSRLSKEQQLEVISRTFHESI
>Mature_742_residues
MFKQWEGFKNGTWQEGIDVRNFIQKNYNVYEGDSSFLEGISEKTSRVWDKAYELIVEEVKKGIIDIATDRVSGINNYEAG
YIDKDNEVIVGLQTDAPLKRIVNPFGGFRMVQTSLKEYGYELDKDIEKHFSRYRKTHNEGVFDGYTKEIRLARTAGLLTG
LPDAYGRGRIIGDYRRVALYGIDYLIEEKKNDLDNLQGDMLDELVRKREEVSMQIRALGEIKEMAAKYGCDISKPASNAK
EAVQALYFGYLAGIKENNGAATSFGRTSTFLDIYIERDLETGLITEKEAQELVDQLIIKLRLVRHLRTPEYNDLFGGDPT
WVTESIGGMGINGKSLVTKNSFRYLHTLINLGASAEPNLTVLWSDKLPENFKKYCAEISIKTDAVQYESDEVMRPIYGDD
YAIACCVSAMKVGKQMQFFGARCNIAKSLLYAINGGCDELKGMKVIPGIEVMNDEVLDFNKVKENYFKVLEYVAKIYVDT
MNIIHHMHDKYAYEAGQMALHDTMVDRLMAFGIAGLSVAIDSLSAIKYAKVKPIRNEEGLAIDFEVQGDFPKYGNDDDRA
DDLGVELVTKFSNELKRHPLYRDAKHTLSALTITSNVMYGKKTGTTPDGRKKGEPLAPGANPMHGRDVNGALASLNSVAK
IPYNEVCQDGVSNTFSIVPDALGKSEEQRISNLVSILDGYFVQGAHHLNVNVLNRETLIDAMENPDKYPTLTIRVSGYAV
NFSRLSKEQQLEVISRTFHESI

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=750, Percent_Identity=61.4666666666667, Blast_Score=957, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=750, Percent_Identity=57.6, Blast_Score=899, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=597, Percent_Identity=28.9782244556114, Blast_Score=187, Evalue=3e-48,
Organism=Escherichia coli, GI1790388, Length=727, Percent_Identity=24.6217331499312, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1788933, Length=61, Percent_Identity=67.2131147540984, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 83353; Mature: 83353

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKQWEGFKNGTWQEGIDVRNFIQKNYNVYEGDSSFLEGISEKTSRVWDKAYELIVEEVK
CCCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGIIDIATDRVSGINNYEAGYIDKDNEVIVGLQTDAPLKRIVNPFGGFRMVQTSLKEYGY
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHCC
ELDKDIEKHFSRYRKTHNEGVFDGYTKEIRLARTAGLLTGLPDAYGRGRIIGDYRRVALY
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHH
GIDYLIEEKKNDLDNLQGDMLDELVRKREEVSMQIRALGEIKEMAAKYGCDISKPASNAK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
EAVQALYFGYLAGIKENNGAATSFGRTSTFLDIYIERDLETGLITEKEAQELVDQLIIKL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
RLVRHLRTPEYNDLFGGDPTWVTESIGGMGINGKSLVTKNSFRYLHTLINLGASAEPNLT
HHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCCEE
VLWSDKLPENFKKYCAEISIKTDAVQYESDEVMRPIYGDDYAIACCVSAMKVGKQMQFFG
EEECCCCCHHHHHHHHHCCEECCCEEECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARCNIAKSLLYAINGGCDELKGMKVIPGIEVMNDEVLDFNKVKENYFKVLEYVAKIYVDT
HHHHHHHHHHHHHCCCHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MNIIHHMHDKYAYEAGQMALHDTMVDRLMAFGIAGLSVAIDSLSAIKYAKVKPIRNEEGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AIDFEVQGDFPKYGNDDDRADDLGVELVTKFSNELKRHPLYRDAKHTLSALTITSNVMYG
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCEEEC
KKTGTTPDGRKKGEPLAPGANPMHGRDVNGALASLNSVAKIPYNEVCQDGVSNTFSIVPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHCCH
ALGKSEEQRISNLVSILDGYFVQGAHHLNVNVLNRETLIDAMENPDKYPTLTIRVSGYAV
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHCCCCCCCEEEEEEECEEE
NFSRLSKEQQLEVISRTFHESI
EHHHHCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFKQWEGFKNGTWQEGIDVRNFIQKNYNVYEGDSSFLEGISEKTSRVWDKAYELIVEEVK
CCCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGIIDIATDRVSGINNYEAGYIDKDNEVIVGLQTDAPLKRIVNPFGGFRMVQTSLKEYGY
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHCC
ELDKDIEKHFSRYRKTHNEGVFDGYTKEIRLARTAGLLTGLPDAYGRGRIIGDYRRVALY
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHH
GIDYLIEEKKNDLDNLQGDMLDELVRKREEVSMQIRALGEIKEMAAKYGCDISKPASNAK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
EAVQALYFGYLAGIKENNGAATSFGRTSTFLDIYIERDLETGLITEKEAQELVDQLIIKL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
RLVRHLRTPEYNDLFGGDPTWVTESIGGMGINGKSLVTKNSFRYLHTLINLGASAEPNLT
HHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCCEE
VLWSDKLPENFKKYCAEISIKTDAVQYESDEVMRPIYGDDYAIACCVSAMKVGKQMQFFG
EEECCCCCHHHHHHHHHCCEECCCEEECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARCNIAKSLLYAINGGCDELKGMKVIPGIEVMNDEVLDFNKVKENYFKVLEYVAKIYVDT
HHHHHHHHHHHHHCCCHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MNIIHHMHDKYAYEAGQMALHDTMVDRLMAFGIAGLSVAIDSLSAIKYAKVKPIRNEEGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AIDFEVQGDFPKYGNDDDRADDLGVELVTKFSNELKRHPLYRDAKHTLSALTITSNVMYG
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCEEEC
KKTGTTPDGRKKGEPLAPGANPMHGRDVNGALASLNSVAKIPYNEVCQDGVSNTFSIVPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHCCH
ALGKSEEQRISNLVSILDGYFVQGAHHLNVNVLNRETLIDAMENPDKYPTLTIRVSGYAV
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHCCCCCCCEEEEEEECEEE
NFSRLSKEQQLEVISRTFHESI
EHHHHCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]