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The map label for this gene is rluF [H]

Identifier: 187935235

GI number: 187935235

Start: 2607621

End: 2608301

Strand: Reverse

Name: rluF [H]

Synonym: CLL_A2511

Alternate gene names: 187935235

Gene position: 2608301-2607621 (Counterclockwise)

Preceding gene: 187933470

Following gene: 187934085

Centisome position: 68.63

GC content: 25.84

Gene sequence:

>681_bases
ATGAGAATAAATAAACTTTTTAGTAATTTAGGTATTTGTTCAAGAAAAGATACTAGTAAACTTATTGAAGAAAATAGAAT
AATAGTTAATGGATTTCCTTGCATACAAGGGCAGTGGGTTGAATTAGAGGATGAAATTCTTCTTGACAATAAACCTCTTC
CAATAAAGAAAAAAATATATATAGTTTTAAATAAGCCTATAGGAATAACATGTACTGCAGCAGATGAGGTTGAAAATAAT
ATAATAAATTTTATGAATTATCCTGAATATATATTTCCAGTTGGAAGATTAGATAAAGCATCTCAAGGGTTAATTTTATT
AACTAATGATGGAGATTTAGCTAATAAAATTTTAGAGTCGGAAAATAAACATGAAAAAGAATATATAGTTACTTTAGATA
AACCATTTAATGATGAATTTATTTTAGGGATGACAAGTGGTGTTGAAATATGTAATGTAAAAACAAGGCAATGCAAGGTT
AGAAGAATTTCAGAAGATACATTTAATATTGTTTTAACGCAAGGACTTAATAAGCAAATTAGAAGAATGAGTAAAACATT
TGGTTATACTGTTAAAAAATTAGAAAGAATACGAATTGTTAATATTGAACTTGGAGATTTAGAATATGGTAAATGGCGTT
ATTTATTGGAAGAAGAGTTAGCAGTATTAAGAAAATTCTAA

Upstream 100 bases:

>100_bases
AAAGTAGAATTTATTAGTGGAGTGTTATTAAATTATACATAATTTAATGTAGTATGTTTTTAGGTGTTAAAGTTAAACTT
TTAAAGTTGGGAGACAAGAT

Downstream 100 bases:

>100_bases
GTAATAAAAAATTACCAAGTAGACTTTTAGAATATTATATGTACATATAGGTTTATATTTTATTAAAATGGTTATATATA
CATGAACAAGGTGAATGTAT

Product: ribosomal large subunit pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MRINKLFSNLGICSRKDTSKLIEENRIIVNGFPCIQGQWVELEDEILLDNKPLPIKKKIYIVLNKPIGITCTAADEVENN
IINFMNYPEYIFPVGRLDKASQGLILLTNDGDLANKILESENKHEKEYIVTLDKPFNDEFILGMTSGVEICNVKTRQCKV
RRISEDTFNIVLTQGLNKQIRRMSKTFGYTVKKLERIRIVNIELGDLEYGKWRYLLEEELAVLRKF

Sequences:

>Translated_226_residues
MRINKLFSNLGICSRKDTSKLIEENRIIVNGFPCIQGQWVELEDEILLDNKPLPIKKKIYIVLNKPIGITCTAADEVENN
IINFMNYPEYIFPVGRLDKASQGLILLTNDGDLANKILESENKHEKEYIVTLDKPFNDEFILGMTSGVEICNVKTRQCKV
RRISEDTFNIVLTQGLNKQIRRMSKTFGYTVKKLERIRIVNIELGDLEYGKWRYLLEEELAVLRKF
>Mature_226_residues
MRINKLFSNLGICSRKDTSKLIEENRIIVNGFPCIQGQWVELEDEILLDNKPLPIKKKIYIVLNKPIGITCTAADEVENN
IINFMNYPEYIFPVGRLDKASQGLILLTNDGDLANKILESENKHEKEYIVTLDKPFNDEFILGMTSGVEICNVKTRQCKV
RRISEDTFNIVLTQGLNKQIRRMSKTFGYTVKKLERIRIVNIELGDLEYGKWRYLLEEELAVLRKF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=229, Percent_Identity=47.1615720524017, Blast_Score=202, Evalue=1e-53,
Organism=Escherichia coli, GI1788510, Length=228, Percent_Identity=31.140350877193, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787524, Length=227, Percent_Identity=27.7533039647577, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87081838, Length=172, Percent_Identity=23.8372093023256, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26203; Mature: 26203

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINKLFSNLGICSRKDTSKLIEENRIIVNGFPCIQGQWVELEDEILLDNKPLPIKKKIY
CCHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCCCCEEEEECCEEEECCCCCCCCEEEE
IVLNKPIGITCTAADEVENNIINFMNYPEYIFPVGRLDKASQGLILLTNDGDLANKILES
EEEECCCCEEEEEHHHHHHHHHHHCCCCHHEEECCCCCCCCCCEEEEECCCHHHHHHHHC
ENKHEKEYIVTLDKPFNDEFILGMTSGVEICNVKTRQCKVRRISEDTFNIVLTQGLNKQI
CCCCCCEEEEEECCCCCCCEEEEECCCCEEECCCHHHEEEEEECCCCEEEEEECCCCHHH
RRMSKTFGYTVKKLERIRIVNIELGDLEYGKWRYLLEEELAVLRKF
HHHHHHHCHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRINKLFSNLGICSRKDTSKLIEENRIIVNGFPCIQGQWVELEDEILLDNKPLPIKKKIY
CCHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCCCCEEEEECCEEEECCCCCCCCEEEE
IVLNKPIGITCTAADEVENNIINFMNYPEYIFPVGRLDKASQGLILLTNDGDLANKILES
EEEECCCCEEEEEHHHHHHHHHHHCCCCHHEEECCCCCCCCCCEEEEECCCHHHHHHHHC
ENKHEKEYIVTLDKPFNDEFILGMTSGVEICNVKTRQCKVRRISEDTFNIVLTQGLNKQI
CCCCCCEEEEEECCCCCCCEEEEECCCCEEECCCHHHEEEEEECCCCEEEEEECCCCHHH
RRMSKTFGYTVKKLERIRIVNIELGDLEYGKWRYLLEEELAVLRKF
HHHHHHHCHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]