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The map label for this gene is pfkA [H]

Identifier: 187934895

GI number: 187934895

Start: 3511339

End: 3512301

Strand: Reverse

Name: pfkA [H]

Synonym: CLL_A3337

Alternate gene names: 187934895

Gene position: 3512301-3511339 (Counterclockwise)

Preceding gene: 187935605

Following gene: 187933683

Centisome position: 92.42

GC content: 34.99

Gene sequence:

>963_bases
ATGAAGAAAATAGCTATTTTAACAAGTGGTGGAGATGCCCCAGGAATGAACGCAGCTATAAGAGCAGTAGTAAGAACAGC
CATTGACAAAGGACTTGAAGTTATGGGAGTTCAAAGAGGCTACAGTGGATTACTTAATGGTGAACTATTTGCAATGAATA
GAACATCAGTATCTGATATTATTCAAAGAGGTGGAACAATCCTAAGAACTGCTAGATGCCCAGAGTTCAAAGACGAAGAG
GTAAGAAAAAGAGCAGTTAAGATATTAAATGCTTATGGTGTAGATGCTTTAGTTGTTATTGGTGGAGATGGATCTTTTAT
GGGAGCCAAACTACTATCAAAACTAGGAATCAAAACTATAGGATTACCAGGAACAATAGATAATGATTTAGCTTATACTG
ATTTCACAATAGGATTTGATACAGCATTAAATACTATTGTTGATGCAATAAATAAAATAAGAGATACATCAACTTCTCAT
GAAAGAGTTTCAATAATTGAAGTTATGGGAAGAGATTGTGGAGACTTAGCTCTTCATGCTGGTATATCAAGTGGAGCAGA
AGCTATAATAGTTCCAGAAATGGGTGAATTCGATAGAGATGCACTTTGCAGAACTATATTAGATGGTAAAAACCATGGAA
AAACTCATAGCATAGTTATTCTTGCAGAAGGAATTGGTGGAGCAGAAGAACTATCAAAATATGTTCAAGAATTAACAGGA
ATAGAAGCAAGAGCTACAATATTAGGTCATATCCAAAGAGGTGGAGCACCATCTGCATCAGATAGAGTTTTAGCTTCAAG
ATTGGGAGCTAGAGCAGTAGAAGTTCTTTTACAAGGAGAAACTTCAAGAGTAATAGGAATTAGAGACAATCAAATTGTAG
ACCAAGATATAGATGAAGCATTAGCTATAGAAAGTAAGTTTGACTTAGATCTATATAATGTAGCAGAAGTATTATCACGC
TAA

Upstream 100 bases:

>100_bases
TATTTTTATGTACAATTATTAGTGGTTAATTAAGACATGCATAAACTATGCGCAGGTAGGACTTTGGGTGTCCTAGTTGT
TGTTTAAGGAGGAATATATT

Downstream 100 bases:

>100_bases
TAAATATTTATTTATATAAATTTTTATAAACTAATTAGAATAAAGAGGAGTGTACTTACATGAGAAAGACTAAAATGATT
TGTACTATTGGACCAGCAAG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKKIAILTSGGDAPGMNAAIRAVVRTAIDKGLEVMGVQRGYSGLLNGELFAMNRTSVSDIIQRGGTILRTARCPEFKDEE
VRKRAVKILNAYGVDALVVIGGDGSFMGAKLLSKLGIKTIGLPGTIDNDLAYTDFTIGFDTALNTIVDAINKIRDTSTSH
ERVSIIEVMGRDCGDLALHAGISSGAEAIIVPEMGEFDRDALCRTILDGKNHGKTHSIVILAEGIGGAEELSKYVQELTG
IEARATILGHIQRGGAPSASDRVLASRLGARAVEVLLQGETSRVIGIRDNQIVDQDIDEALAIESKFDLDLYNVAEVLSR

Sequences:

>Translated_320_residues
MKKIAILTSGGDAPGMNAAIRAVVRTAIDKGLEVMGVQRGYSGLLNGELFAMNRTSVSDIIQRGGTILRTARCPEFKDEE
VRKRAVKILNAYGVDALVVIGGDGSFMGAKLLSKLGIKTIGLPGTIDNDLAYTDFTIGFDTALNTIVDAINKIRDTSTSH
ERVSIIEVMGRDCGDLALHAGISSGAEAIIVPEMGEFDRDALCRTILDGKNHGKTHSIVILAEGIGGAEELSKYVQELTG
IEARATILGHIQRGGAPSASDRVLASRLGARAVEVLLQGETSRVIGIRDNQIVDQDIDEALAIESKFDLDLYNVAEVLSR
>Mature_320_residues
MKKIAILTSGGDAPGMNAAIRAVVRTAIDKGLEVMGVQRGYSGLLNGELFAMNRTSVSDIIQRGGTILRTARCPEFKDEE
VRKRAVKILNAYGVDALVVIGGDGSFMGAKLLSKLGIKTIGLPGTIDNDLAYTDFTIGFDTALNTIVDAINKIRDTSTSH
ERVSIIEVMGRDCGDLALHAGISSGAEAIIVPEMGEFDRDALCRTILDGKNHGKTHSIVILAEGIGGAEELSKYVQELTG
IEARATILGHIQRGGAPSASDRVLASRLGARAVEVLLQGETSRVIGIRDNQIVDQDIDEALAIESKFDLDLYNVAEVLSR

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=353, Percent_Identity=40.2266288951841, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI266453748, Length=353, Percent_Identity=40.2266288951841, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI4505749, Length=353, Percent_Identity=40.2266288951841, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI266453619, Length=353, Percent_Identity=40.2266288951841, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI48762920, Length=339, Percent_Identity=39.8230088495575, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI11321601, Length=311, Percent_Identity=40.5144694533762, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1790350, Length=319, Percent_Identity=53.6050156739812, Blast_Score=339, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=41.8006430868167, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17558788, Length=312, Percent_Identity=36.2179487179487, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI72003601, Length=315, Percent_Identity=29.5238095238095, Blast_Score=127, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6321679, Length=355, Percent_Identity=40.5633802816901, Blast_Score=206, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6323861, Length=342, Percent_Identity=38.0116959064327, Blast_Score=202, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24652337, Length=329, Percent_Identity=41.9452887537994, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI17647809, Length=329, Percent_Identity=41.9452887537994, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI28573326, Length=329, Percent_Identity=40.7294832826748, Blast_Score=218, Evalue=4e-57,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34089; Mature: 34089

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAILTSGGDAPGMNAAIRAVVRTAIDKGLEVMGVQRGYSGLLNGELFAMNRTSVSDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEEHHHCCCCCCCCEEEEECCCCHHHH
IQRGGTILRTARCPEFKDEEVRKRAVKILNAYGVDALVVIGGDGSFMGAKLLSKLGIKTI
HHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEE
GLPGTIDNDLAYTDFTIGFDTALNTIVDAINKIRDTSTSHERVSIIEVMGRDCGDLALHA
CCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHC
GISSGAEAIIVPEMGEFDRDALCRTILDGKNHGKTHSIVILAEGIGGAEELSKYVQELTG
CCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHC
IEARATILGHIQRGGAPSASDRVLASRLGARAVEVLLQGETSRVIGIRDNQIVDQDIDEA
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHH
LAIESKFDLDLYNVAEVLSR
HHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKIAILTSGGDAPGMNAAIRAVVRTAIDKGLEVMGVQRGYSGLLNGELFAMNRTSVSDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEEHHHCCCCCCCCEEEEECCCCHHHH
IQRGGTILRTARCPEFKDEEVRKRAVKILNAYGVDALVVIGGDGSFMGAKLLSKLGIKTI
HHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEE
GLPGTIDNDLAYTDFTIGFDTALNTIVDAINKIRDTSTSHERVSIIEVMGRDCGDLALHA
CCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHC
GISSGAEAIIVPEMGEFDRDALCRTILDGKNHGKTHSIVILAEGIGGAEELSKYVQELTG
CCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHC
IEARATILGHIQRGGAPSASDRVLASRLGARAVEVLLQGETSRVIGIRDNQIVDQDIDEA
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHH
LAIESKFDLDLYNVAEVLSR
HHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA