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The map label for this gene is pyk [H]

Identifier: 187933683

GI number: 187933683

Start: 3509864

End: 3511279

Strand: Reverse

Name: pyk [H]

Synonym: CLL_A3336

Alternate gene names: 187933683

Gene position: 3511279-3509864 (Counterclockwise)

Preceding gene: 187934895

Following gene: 187932917

Centisome position: 92.39

GC content: 34.46

Gene sequence:

>1416_bases
ATGAGAAAGACTAAAATGATTTGTACTATTGGACCAGCAAGTGAAGACAGAGAAATATTAGAACAAGTAATGTTAGCAGG
AATGAATGCTTCAAGACATAACTTTTCACATGGTGATCACGAAGAACATAGAGGAAGAATAGAAAAGGTTAAAGAATTAT
CAAAGAAACTTGATAAAGAAATAGCTATCATCCTTGATACAAAAGGACCAGAAATAAGAACTGGAAAATTCGAACCAAAC
AAAGTTGAATTAGTTAAAGGTACAGAATTTACTGTATACGCAGGAGATATGTCAGTAGTTGGAGACACTACAAAGTGTTC
AGTTACTTACGAAGGATTAGCTAATGATGTTAAAGCAGGAAACACAATCTTAATAGATGATGGTTTAGTAGGATTAACAG
TTAAATCAGTAAAAGGAAACGCAGTTATCTGTGAAGTTCAAAATACTGGATTAGTTGGAACTCATAAAGGCGTTAACGTA
CCAGGAGTTTCAATCCAATTACCAGCTCTTACAGACAAAGATAAATCAGATTTAATATTTGGTTGTGAAATGGGAGTTAC
TATGATAGCAGCTTCATTCGTAAGAAAAGCAGCTGACGTAGTAGCTATAAGAAAAGTTCTTGATGAAAATGGTGGAAAGA
ACATCTTAATCTGCCCTAAAATCGAAAACCAAGAAGGTGTTGATAACATCGATTCAATATTAGAAATCTCAGATGCTATA
ATGGTAGCTAGAGGAGATCTAGGTGTTGAAATTCCAATAGAACAAGTACCAGCAGTTCAAAAAATGATCATTCAAAAATG
TAATGCAGCTGGAAAGCCAGTTGTTACTGCAACTCAAATGTTAGATTCTATGATCAGAAACCCAAGACCAACAAGAGCAG
AAGTTTCAGATGTAGCTAACGCTATATTAGATGGTACTGATGCAATAATGTTATCAGGAGAAAGTGCTAACGGAACTTAC
CCAGTAGAAGCTGTTAGAACTATGGCTAAAATTGCTGAAGAAACTGAAAAGCAATTAGCTCATAAAGTTGCTTACTCAAA
TGATAAATCTTCAATATCTGAAGTAATTTCAAGAGCAGCATGTAATGCAGCTAATGAATTAGAAGCAACTGCTATAATTT
CTTCAACTCAAACAGGAGCTACTGCTAAGAGAATATCTAAGTCTAGACCAGATTGTACAATCATAGCTGTTACTCCAGAT
GAAGTAGTAGCAAAACAATTAGCATTCTCATGGGGAGTTTACCCAATAGTTGCTGATAAAATGTGTTCAACTGACGAAAT
GATGATAAAATCAGTTGAAATAGCAAAAGAACATAACTATGTTAAAAATGGTGATACAGTAGTTTTAGCTGCTGGAGTTC
CAGTTGATAAAACAGGAACAACTAACCTATTAAAAGTTAGCGTAGTAGGCGAATAA

Upstream 100 bases:

>100_bases
ACTTAGATCTATATAATGTAGCAGAAGTATTATCACGCTAATAAATATTTATTTATATAAATTTTTATAAACTAATTAGA
ATAAAGAGGAGTGTACTTAC

Downstream 100 bases:

>100_bases
TAAACTTATAATAAAAAAAGCTTTCGATTTTATTCGAGAGCTTTTTTATTCTTTTTATTAACTATACTACTAATAATTGA
GTTGAATAATTAAATATAAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MRKTKMICTIGPASEDREILEQVMLAGMNASRHNFSHGDHEEHRGRIEKVKELSKKLDKEIAIILDTKGPEIRTGKFEPN
KVELVKGTEFTVYAGDMSVVGDTTKCSVTYEGLANDVKAGNTILIDDGLVGLTVKSVKGNAVICEVQNTGLVGTHKGVNV
PGVSIQLPALTDKDKSDLIFGCEMGVTMIAASFVRKAADVVAIRKVLDENGGKNILICPKIENQEGVDNIDSILEISDAI
MVARGDLGVEIPIEQVPAVQKMIIQKCNAAGKPVVTATQMLDSMIRNPRPTRAEVSDVANAILDGTDAIMLSGESANGTY
PVEAVRTMAKIAEETEKQLAHKVAYSNDKSSISEVISRAACNAANELEATAIISSTQTGATAKRISKSRPDCTIIAVTPD
EVVAKQLAFSWGVYPIVADKMCSTDEMMIKSVEIAKEHNYVKNGDTVVLAAGVPVDKTGTTNLLKVSVVGE

Sequences:

>Translated_471_residues
MRKTKMICTIGPASEDREILEQVMLAGMNASRHNFSHGDHEEHRGRIEKVKELSKKLDKEIAIILDTKGPEIRTGKFEPN
KVELVKGTEFTVYAGDMSVVGDTTKCSVTYEGLANDVKAGNTILIDDGLVGLTVKSVKGNAVICEVQNTGLVGTHKGVNV
PGVSIQLPALTDKDKSDLIFGCEMGVTMIAASFVRKAADVVAIRKVLDENGGKNILICPKIENQEGVDNIDSILEISDAI
MVARGDLGVEIPIEQVPAVQKMIIQKCNAAGKPVVTATQMLDSMIRNPRPTRAEVSDVANAILDGTDAIMLSGESANGTY
PVEAVRTMAKIAEETEKQLAHKVAYSNDKSSISEVISRAACNAANELEATAIISSTQTGATAKRISKSRPDCTIIAVTPD
EVVAKQLAFSWGVYPIVADKMCSTDEMMIKSVEIAKEHNYVKNGDTVVLAAGVPVDKTGTTNLLKVSVVGE
>Mature_471_residues
MRKTKMICTIGPASEDREILEQVMLAGMNASRHNFSHGDHEEHRGRIEKVKELSKKLDKEIAIILDTKGPEIRTGKFEPN
KVELVKGTEFTVYAGDMSVVGDTTKCSVTYEGLANDVKAGNTILIDDGLVGLTVKSVKGNAVICEVQNTGLVGTHKGVNV
PGVSIQLPALTDKDKSDLIFGCEMGVTMIAASFVRKAADVVAIRKVLDENGGKNILICPKIENQEGVDNIDSILEISDAI
MVARGDLGVEIPIEQVPAVQKMIIQKCNAAGKPVVTATQMLDSMIRNPRPTRAEVSDVANAILDGTDAIMLSGESANGTY
PVEAVRTMAKIAEETEKQLAHKVAYSNDKSSISEVISRAACNAANELEATAIISSTQTGATAKRISKSRPDCTIIAVTPD
EVVAKQLAFSWGVYPIVADKMCSTDEMMIKSVEIAKEHNYVKNGDTVVLAAGVPVDKTGTTNLLKVSVVGE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=487, Percent_Identity=44.1478439425051, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI32967597, Length=487, Percent_Identity=44.1478439425051, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=43.1211498973306, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=43.1211498973306, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=43.3264887063655, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=47.5694444444444, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=47.5694444444444, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=44.0677966101695, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=44.0677966101695, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI310128738, Length=212, Percent_Identity=43.3962264150943, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=53.3898305084746, Blast_Score=490, Evalue=1e-140,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=38.9121338912134, Blast_Score=302, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17544584, Length=482, Percent_Identity=44.6058091286307, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=44.4214876033058, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=44.4214876033058, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=44.4214876033058, Blast_Score=359, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=44.4214876033058, Blast_Score=359, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=43.4156378600823, Blast_Score=345, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6324923, Length=488, Percent_Identity=41.1885245901639, Blast_Score=335, Evalue=7e-93,
Organism=Drosophila melanogaster, GI24648964, Length=498, Percent_Identity=45.5823293172691, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI28571814, Length=498, Percent_Identity=45.5823293172691, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24648966, Length=412, Percent_Identity=44.9029126213592, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24581235, Length=484, Percent_Identity=34.504132231405, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24646914, Length=383, Percent_Identity=37.0757180156658, Blast_Score=206, Evalue=3e-53,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50349; Mature: 50349

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKMICTIGPASEDREILEQVMLAGMNASRHNFSHGDHEEHRGRIEKVKELSKKLDKE
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCE
IAIILDTKGPEIRTGKFEPNKVELVKGTEFTVYAGDMSVVGDTTKCSVTYEGLANDVKAG
EEEEEECCCCCEECCCCCCCEEEEEECCEEEEEECCEEEECCCEEEEEECCCCCCHHCCC
NTILIDDGLVGLTVKSVKGNAVICEVQNTGLVGTHKGVNVPGVSIQLPALTDKDKSDLIF
CEEEEECCEEEEEEEECCCCEEEEEEECCCEEECCCCCCCCCCEEEECCCCCCCCCCEEE
GCEMGVTMIAASFVRKAADVVAIRKVLDENGGKNILICPKIENQEGVDNIDSILEISDAI
ECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCEE
MVARGDLGVEIPIEQVPAVQKMIIQKCNAAGKPVVTATQMLDSMIRNPRPTRAEVSDVAN
EEEECCCCEECCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
AILDGTDAIMLSGESANGTYPVEAVRTMAKIAEETEKQLAHKVAYSNDKSSISEVISRAA
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CNAANELEATAIISSTQTGATAKRISKSRPDCTIIAVTPDEVVAKQLAFSWGVYPIVADK
CCCCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCHHHHHHH
MCSTDEMMIKSVEIAKEHNYVKNGDTVVLAAGVPVDKTGTTNLLKVSVVGE
HCCCHHHHHHHHHHHHHCCEECCCCEEEEEECCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MRKTKMICTIGPASEDREILEQVMLAGMNASRHNFSHGDHEEHRGRIEKVKELSKKLDKE
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCE
IAIILDTKGPEIRTGKFEPNKVELVKGTEFTVYAGDMSVVGDTTKCSVTYEGLANDVKAG
EEEEEECCCCCEECCCCCCCEEEEEECCEEEEEECCEEEECCCEEEEEECCCCCCHHCCC
NTILIDDGLVGLTVKSVKGNAVICEVQNTGLVGTHKGVNVPGVSIQLPALTDKDKSDLIF
CEEEEECCEEEEEEEECCCCEEEEEEECCCEEECCCCCCCCCCEEEECCCCCCCCCCEEE
GCEMGVTMIAASFVRKAADVVAIRKVLDENGGKNILICPKIENQEGVDNIDSILEISDAI
ECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCEE
MVARGDLGVEIPIEQVPAVQKMIIQKCNAAGKPVVTATQMLDSMIRNPRPTRAEVSDVAN
EEEECCCCEECCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
AILDGTDAIMLSGESANGTYPVEAVRTMAKIAEETEKQLAHKVAYSNDKSSISEVISRAA
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CNAANELEATAIISSTQTGATAKRISKSRPDCTIIAVTPDEVVAKQLAFSWGVYPIVADK
CCCCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCHHHHHHH
MCSTDEMMIKSVEIAKEHNYVKNGDTVVLAAGVPVDKTGTTNLLKVSVVGE
HCCCHHHHHHHHHHHHHCCEECCCCEEEEEECCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286; 9625784 [H]